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31	Biology	ABS-186
Antioxidant Capacity of Combined Extract from Galangal, Curcuma and Aromatic Ginger Based on DPPH, ABTS and FRAP Sri Rahayu, Novita Rahma Mujayani, Tri Handayani Kurniati, Sri Sofiati Umami, Jungshan Chang Universitas Negeri Jakarta Abstract Galangal, curcuma and ginger has been long used as traditional jamu. This study Reveal the antioxidant capacity of Galangal, curcuma and ginger based on DPPH, ABTS and FRAP. Method used was complete randomized design with 5 concentration variation of 10,50,100,150 and 200 ppm and 3 combination of 1:1:1, 1:2:1 and 2:1:2 of the extract. Data was analyzed with Anova followed by DMRT. Findings of the study exhibit that all extract consist of flavonoid from the type of Flavonon dan dihidroflavonol. Combination of 2:1:2 of the extract qualitatively showed the strongest phenol and Flavonoid content. Concentration of 200 ppm has most promising inhibition against DPPH, ABTS and FRAP. All samples also showed high IC50 of more that 1.000 and considered to be safely used. Result suggest that combination of the extracts have good antioxidant capacity and can be used as potential candidate for pgarmaceutical products. Keywords: Galangal, curcuma, ginger, radicals, antioxidant Share Link | Plain Format | Corresponding Author (Sri Rahayu)

32	Biology	ABS-194
Effect of 6-Benzylaminopurine and Repeated Subculturing for in vitro Shoots Production of Banana (Musa acuminata x balbisiana, ABB) Reni Indrayanti,Sunani, Agus Sutanto, Sudarsono, Adisyahputra Universitas Negeri Jakarta Abstract Banana cv. Kepok (Musa acuminata x balbisiana-ABB) is one of the supporting plants for food security. The production of banana cv. Kepok can be achieved through in vitro culture techniques by increasing the concentration of plant growth regulators in repeated subcultures. The study aims to determine the effect of repeated subcultures with the increasing addition of BAP to produce in vitro banana cv. Kepok clones. This research uses an experimental method with a Completely Randomized Design (CRD). Banana aseptic shoots were grown in Murashige and Skoog media containing 0.175 mg L-1 IAA and different concentrations of BAP. The experiment consisted of 3 BAP treatments with concentrations of 4.50 mg L-1 BAP (I1B1), 6.75 mg L-1 BAP ((I1B2), and 9.00 mgL-1 BAP (I1B3). Each stage of BAP was subcultured 1-2 times with six subcultures for six months. The independent T-test results showed a significant effect on the average number of shoots, leaves, and roots of the Kepok cultivar after being subcultured in stages for six months (p<0.05). The number of shoots per explant after multiplication and subculturing to the Murashige and Skoog media supplemented with 0.175 mg L-1 IAA, and increasing concentration of BAP shows that the average number of shoots increased from 3.5 shoots per explant and became 13.9 shoots per explant. The avarage number of leaves increased from 6.2 leaves per explant and became 14 leaves per explant. In addition, the number of roots increased from 0.4 roots per explant became 6.26 roots per explant. This study showed that subculturing banana explants in Murashige and Skoog medium containing BAP with increasing concentration for six months produced 644 explants of banana, which is increased 43fold from initial explants with the average number of shoots per explant being 13.9 shoots. This study concludes that BAP and repeated subculturing can produce in vitro banana shoots. Keywords: Indole 3-acetic acid, genom ABB, Kepok Cultivar, mass production, Murashige and Skoog. Share Link | Plain Format | Corresponding Author (Reni Indrayanti)

33	Biology	ABS-195
MORPHOLOGY IDENTIFICATION AND DESCRIPTION OF BANANA (Musa spp) IN KALIKUNING AND FOREST AREA WITH SPECIAL PURPOSE OF KALIURANG Reni Indrayanti, Septiani Abmilasari, Alma Fahira, Indina Rifdayani, Okta Latifa Zaqiah, Rizal Koen Asharo Universitas Negeri Jakarta Abstract Banana (Musa spp.) is a native Indonesian plant with the characteristics of a large herbaceous plant. The height of a banana varies between 1-4 meters, with broad and long leaves. Wild bananas and cultivated bananas can be found on the island of Java, including in Kalikuning and the Kaliurang Special Purpose Forest Area (Kawasan Hutan Dengan Tujuan Khusus - KHDTK) Kaliurang - Gunung Merapi National Park. There are 325 banana cultivars recorded in Indonesia, and the diversity of banana species needs to be investigated further because it is a source of germplasm, especially in the Kaliurang research forest area. The research method is descriptive with field observations, identifying the types of wild and cultivated bananas found in Gunung Merapi National Park. Parameters observed were morphological characters of banana plants. The results of the field observations found that there are 12 clumps of banana plants in the Kalikuning area and three clumps of banana plants in the KHDTK area of Kaliurang. The morphological identification of banana species based on banana descriptors from the International Plant Genetic Resources Institute (IPGRI) - INIBAP showed that most of the bananas found have Musa acuminata characteristics, except for Musa spp (sample number 6), which showed a characteristic of wild banana Musa balbisiana. Bananas in the Kalikuning area were predicted as Pisang Raja (Musa x paradisiaca AAB). Morphology characteristics of banana Pisang Raja are dull (waxy) pseudostem appearance and erect leaf habit. Pseudostem color is green-lighting, watery sap color, petiole canal leaf III is open with margin spreading, petiole margin color is green to red. The shape of the leaf blade base is both sides pointed. The other banana clumps plant still can not identify because of the lack of some generative phase characteristics. Keywords: . Gunung Merapi National Park, Pisang. Raja, Musa acuminata, Musa balbisiana Share Link | Plain Format | Corresponding Author (Reni Indrayanti)

34	Biology	ABS-196
Growth Rate and Survival of Hawksbill Turtle (Eretmochelys imbricata) in Captivity Sabira Island M. Isnin Noer(a*), Ratna Komala(a), Mega Putri(a), Anandhita Arnelya(b), Dwi Ayu Komsiatun(b) a) Jl. Rawamangun Muka, RT.11/RW.14, Rawamangun, Pulo Gadung, Kota Jakarta Timur, Daerah Khusus Ibukota Jakarta 13220 b) Faculty of Mathematics and Natural Sciences Abstract Hawksbill turtles (Eretmochelys imbricata) are categorized with critically endangered status in the IUCN red list. This status explains that now the population of sea turtles in nature is declining. The decline in population is caused by various threats such as the presence of predators, wild capture, taking of turtle body parts, and the influence of environmental factors. This study aims to determine the growth rate and survival of hawksbill turtle hatchlings in captivity, as well as to determine the environmental factors that affect. The method used is descriptive with observation techniques. The data were analyzed with Principle Component Analysis (PCA) using the SPSS (Statistical Product and Service Solutions) program Version 20.0. The stages of research consist of, observation of hatchling growth, observation of hatchling survival, observation of environmental factors of water quality. The results of the study on the growth rate and survival of hatchlings resulted in a growth rate value (SGR) of 3.23% and survival (SR) of 48%. With an increase in the length of the carapace by 20%, the width of the carapace by 20%, as well as the body weight of the hatchling by 35%. The results of the study showed that the growth rate and survival of hawksbill turtle hatchlings in Sabira Island breeding are still relatively low in management in captivity, because there are still hatchlings that die during the study. Based on the results of the PCA analysis, the factors that most influence the growth rate and survival of hawksbill turtle hatchlings are feed and water salinity. Keywords: Hawksbill turtle- Growth rate- Survival- Captivity Share Link | Plain Format | Corresponding Author (Mega Putri)

35	Biology	ABS-197
Assessment of a Poly&#64258-uorinated and Platinum-Based Analogue against Triple-Negative Breast Cancer Tzu Chun Kan (1), Sonjid Orchirbat (2), Jungshan Chang (1,2,3*) 1) Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan. 2) International Master/Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. 3) International Ph.D. Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. * Correspondence Abstract Breast cancer is the most common cause of deaths in women suffered from cancers in the 20th century. Especially in patients with triple-negative breast cancer (TNBC), they displayed higher recurrence rate and more aggressive stage after diagnosis. DNA-damaging agents, such as cisplatin, served as a successful approach against breast cancer. However, drug resistance has been reported in clinical practice after a long-term course of treatments. To investigate a new medicine with the improved therapeutic efficacy and declined side effects, we performed a new platinum-based compound with polyfluorinated modification and bipyridine structure in this study. The anti-cancer effects of the potential candidate, dichloro [4,4^-bis(2,2,3,3-tetrafluoropropoxy) methyl)-2,2^-bipryridine] platinum (TFBPC), were majorly determined in MDA-MB-231 human breast cancer cells and xenograft models. Results demonstrated that TFBPC targeting to calf-thymus DNA and circular plasmids resulted in the formation of DNA crosslinks, DNA degradation and cell death program through PARP/Bax/Bcl-2 apoptosis and LC3-related autophagy pathway. In cell line-derived xenograft models, TFBPC significantly inhibited tumor growth without the induction of pathological changes in hematological and biochemistry tests. TFBPC also decreased the expressions of P53, Ki-67 and PD-L1 and increased cleaved caspase 3 expressions in tumors. These results revealed a new achievement to overcome cisplatin resistance and to be a potential strategy in patients with triple-negative breast cancer. For further investigations, details in drug-DNA interaction and pharmacokinetics could be conducted. Keywords: cisplatin- xenograft model- triple-negative breast cancers- apoptosis- autophagy Share Link | Plain Format | Corresponding Author (Tzu-Chun Kan)

36	Biology	ABS-198
Alpha 9-Ncotinic Acetylcholine Receptors mediate angiogenesis in triple negative breast cancers Sonjid Ochirbat (1), Tzu-Hsuan Huang (2), Tzu-Chun Kan (2), Jungshan Chang (1,2,3*) 1) International Master/Ph.D Program in Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan 2) Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan 3) International Ph.D Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan Abstract Triple-negative breast cancer (TNBC) remains a significant clinical challenge because of its high vascularity and metastatic rates. Due to lack of surface targeted molecules, chemotherapy is the major strategy for treatments of TNBC patients, contributing to adversary side effects. Previous epidemiological cohort studies demonstrated that both active and passive smoking are associated with risk factors for developing breast cancer. Recently, several studies found that nicotinic acetylcholine receptors &#593-9 (&#593-9-nAChRs) is highly expressed on breast tumors, especially tumors derived from patients diagnosed at advanced stage. In vitro studies, it demonstrated that activation of &#593-9-nAChRs promote carcinogenic behavior including prolonging cell survival, proliferation, increased angiogenesis and metastasis. Therefore, we hypothesized that anti-&#593-9-nAChRs antibodies can be a putative therapeutic drug for TNBC treatment. In this study, we evaluated therapeutic effect of new bispecific antibody with two antigen-binding sites against &#593-9-nAChR and methoxy-polyethylene glycol (mPEG), which provides the linkage to commercial chemotherapy drug, liposomal doxorubicin (Lipo-Dox). Our results showed that anti-&#945-9 and anti-mPEG (&#945--&#945-9-&#945-PEG) bispecific antibody decreased nicotine-induced angiogenic ability on both HUVECs and cancer cells. To characterize molecular mechanism in cancer cells, &#945--&#945-9-&#945-PEG antibody inhibits nicotine induced angiogenesis through downregulating VEGF-A and its receptors (VEGFR2) expression. As well as, the impact of &#593--&#593-9-&#593-PEG bispecific antibody was assessed on cancer cell motility. The nicotine-induced MDA-MB-231 cell migration and invasion were significantly decreased in a dose dependent manner by both transwell chamber assay and wound healing assay. Taken together, results showed that &#593--&#593-9-&#593-PEG bispecific antibody inhibits TNBC progression through suppressing the cancer cell migration, invasion and angiogenesis. We suggest that &#593--&#593-9-&#593-PEG bispecific antibody as Keywords: TNBC- &#593-9-nAChRs- angiogenesis- bispecific antibody Share Link | Plain Format | Corresponding Author (Sonjid Ochirbat)

37	Biology	ABS-199
Analysis of Cellular Immune Response after SARS-CoV-2 Vaccination as An Alternative for Evaluation of Vaccination Success Khariri (1,2), Telly Purnamasari Agus (2*), Tonang Dwi Ardyanto (3), Amin Subandrio (4) 1) Doctoral Program of Biomedical Science, Faculty of Medicine, Indonesia University, Indonesia 2) National Research and Innovation Agency, Indonesia 3) Clinical Pathology Department, Faculty of Medicine, Sebelas Maret University, Indonesia 4) Clinical Microbiology Department, Faculty of Medicine, Indonesia University, Indonesia *Corresponding author: telly2pramadi Abstract Coronavirus Disease (Covid-19) was first discovered in December 2019 in Wuhan and has spread to almost all countries, making it an international health problem. On March 11, 2020, the World Health Organization (WHO) declared Covid-19 as a pandemic. In Indonesia, the first case was announced to the public on March 2, 2020. So far, there is no effective treatment for Covid-19 patients. One of the efforts to prevent Covid-19 infection can be done by vaccination. With vaccination, the body is trained to form an immune response to infection by introducing specific substances that can be recognized by the body. The Covid-19 vaccination needs to be given repeatedly because the humoral immune response only lasts a short time. Adaptive immunity to the longer-lasting Covid-19 vaccine is cellularly mediated by memory T cells. With the prediction that antibody titers will decrease with time since vaccination, an appropriate vaccine administration strategy is needed and the vaccination rate continues to increase in order to achieve this target. Time is a very important factor when using antibody titers as a guide in developing a vaccination program. The analysis is an interesting thing to do to see whether T cells produced from vaccination can also provide longer adaptive immunity against viral infections so that they can be used as an alternative to evaluate the success of vaccination. Keywords: antibodies- immunity- SARS-CoV-2- memory cells- vaccination Share Link | Plain Format | Corresponding Author (Khariri Khariri)

38	Biology	ABS-200
Properties of Bioactive Ingredient in Jellyfish Collagen Siti Maryam Jasman (1), Nur Izyan Wan Azelee (1,2*), Norhayati Mohamed Noora (1), Lim Hui Chingc (3) 1) Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor Bahru, Johor Darul Takzim, Malaysia 2) School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia. 3) MM Cosmetic Sdn. Bhd. No. 4, Jalan Persiaran Maju Cemerlang, Taman Perindustrian Maju Cemerlang, 81100 Ulu Tiram, Johor. *Corresponding author: nur.izyan[at]utm.ibd.my Abstract Keywords: collagen, jellyfish, bioactive ingredient Share Link | Plain Format | Corresponding Author (Siti Maryam Jasman)

39	Biology	ABS-203
Isolation of Pathogenic Bacteria and Initial Resistance Assay with Detached Fruit Assay Method on Orange Fruits (Citrus sp.) Rizal Koen Asharo, Nabilah Destiyana, Rahmah Aulia Azzahrah, Noer Syahbani, Riska Azzahra, Imam Safir Alwan Nurza, Reni Indrayanti Biology Study Program, Universitas Negeri Jakarta Abstract Orange fruit (Citrus sp.) is a horticultural crop which is a leading commodity in Indonesia. Pontianak oranges (Citrus nobilis Lour.) and squeezed oranges (Citrus x sinensis) are oranges that have many consumers. Pontianak oranges are widely known and recognized for their distinctive taste, thin skin, and sweet, slightly sour taste. Citrus consumption for households during the 2016-2020 period is projected to increase with an average growth of 2.74% per year. Diseases that attack citrus fruits are caused by bacteria. The purpose of this study was to isolate and identify disease-causing bacteria in orange fruits which were proven by using Koch^s postulates and their morphological characteristics. The method we use is Fruit detach assay with descriptive analysis. The results of the study showed that the isolates obtained from symptomatic citrus fruits were JMPT-1 isolates with a slimy coccus form. JMPT-1 isolate was able to infect citrus fruits with 50% disease severity and 9% infection rate. Orange fruits were positively infected with citrus pathogenic bacteria due to the presence of black spots, brown spots, soft texture, slimy and unpleasant odor similar to the symptoms of the disease like early squeezed oranges. Keywords: Detached Fruit Assay, disease severity, Koch^s Postulates, orange fruit, pathogenic bacteria Share Link | Plain Format | Corresponding Author (Rizal Koen Asharo)

40	Biology	ABS-204
The Diversity of Moss in Tamiajeng Climbing Trail, Mount Penanggungan, East Java Rizal Koen Asharo, Winda Nurul Fajriah, Muthiah Rahmah Ilahi, Abdul Hakim, Nur Wahyu Fathulhuda, Pinta Omas Pasaribu Biology Study Program, Universitas Negeri Jakarta Abstract Indonesia is a country with a variety of ecosystems, including tropical rainforest areas. The tropical rainforest area is the area where various types of mosses are most often found compared to other areas. Moss is a low-level plant and a group of epiphytic plants that are commonly found on tree trunks, dead wood, weathered wood, soil, and rocks. Moss grows in wet and humid environmental conditions, with sufficient light intensity, and can live well in the lowlands and highlands. Many biodiversity studies have been carried out, but most cases are reported from West Java or Central Java, and a few from East Java. Mount Penanggungan is one of the mountains in East Java with vegetation dominated by mountain forest which has a character like a tropical rain forest. The Tamiajeng trail is the most popular route for climbers, starting from Tamiajeng Village, Trawas, Mojokerto Regency, which is on the southwest side of the mountain. This study was conducted to determine the diversity of mosses on the Tamiajeng hiking trail, Mount Penanggungan, East Java. This study uses an exploratory descriptive method. Moss samples were taken by plotting 30 x 30 m2 on the right and left of the hiking trail for each type of terrestrial and arboreal moss. The results of the study found 12 tribes of mosses with 16 different types of mosses. The value of the Shannon-Wiener Diversity Index is 2.58 in the medium category. The Morishita Index value is between 0.83-1.23 with a uniform and clumped distribution pattern. The Simpson Dominance Index shows that there are 8 dominant species of moss, 7 subdominant species, and 1 non-dominant species. Keywords: distribution pattern, diversity, domination, moss, Mount Penanggungan Share Link | Plain Format | Corresponding Author (Rizal Koen Asharo)

41	Biology	ABS-205
The Role of Tumor Necrosis Factor Alpha (TNF-&#945-), Transforming growth factor-b (TGF-b), and T-cells on Immunity and Tissue Damage Hasta Handayani Idrus (1*), Ratih Rinendyaputri (2), Sunarno (3), Syamsu Rijal (4), Yusriani Mangarengi (5), Masriadi (6), Rasfayanah (7), Hermiaty Nasruddin (8) 1,2,3) The National Research and Innovation Agency, Research Organization For Health,Center For Biomedical Research 1) Department of Microbiology Faculty of Medicine, Universitas Muslim Indonesia, Indonesia 1) Department of Phatology Anatomy, Faculty of Medicine, Universitas Muslim Indonesia, Indonesia 2) Department of Microbiology Faculty of Medicine, Universitas Muslim Indonesia, Indonesia 3) Department of Public Health Faculty of Public Health, Universitas Muslim Indonesia, Indonesia 4) Department Biochemistry Faculty of Medicine, Universitas Muslim Indonesia, Indonesia 5) Department Public Health Faculty of Medicine, Universitas Muslim Indonesia, Indonesia * Corresponding author: hastahandayani[at]umi.ac.id- hasta.handayani.idrus[at]brin.go.id Abstract Tumor necrosis factor alpha (TNF-&#945-) is a pleiotropic cytokine that plays a role in the inflammatory process, initiating polymorphonuclear (PMN) and activating it so that PMNs can reach the site of infection. Tumor necrosis factor alpha (TNF-&#945-) is a major cytokine in the acute inflammatory response to Gram-negative bacteria and other microbes. Severe infections can trigger the production of large amounts of TNF- causing systemic reactions. TNF- has several functions in the inflammatory process, which can increase the pro-thrombotic role and stimulate adhesion molecules from leukocyte cells and induce endothelial cells, play a role in regulating macrophage activity and immune responses in tissues by stimulating growth factors and other cytokines, functioning as a regulator of hematopoietic and comitogen for T cells and B cells as well as the activity of neutrophil cells and macrophages. Transforming growth factor-b (TGF-b) is a pleiotropic cytokine involved in suppressive and inflammatory immune responses. TGF-b alters immunity under various conditions. TGF-b also regulates thymic T-cell selection and maintains homeostasis of the naive T-cell pool. Another role of TGF-b is to inhibit cytotoxic T lymphocyte (CTL), Th1-, and Th2 cell differentiation while promoting peripheral (p)Treg-, Th17-, Th9-, and Tfh cell generation, and tissue residency of T cells in response. to immune challenge. T cells arise from the bone marrow - derived precursors that traffic to the thymus, where the process of development is completed. Within the thymus, T cell precursors are exposed to a variety of extrinsic signals, for example, peptides presented by major histocompatibility complexes (MHC), costimulators, and cytokines, which stimulate molecular changes leading to differentiation into T cell lineages. T-cell precursors require appropriate TCR signaling to trigger their survival and maturation, a process called positive selection. ^Too little^ signaling causes the death of developing T c Keywords: 3 tumor necrosis factor alpha, . transforming growth factor-b, t-cell, immunity, tissue damage Share Link | Plain Format | Corresponding Author (Hasta Handayani Idrus)

42	Biology	ABS-206
Microalgae Diversity in Bogor Botanical Gardens Rizky Priambodo, Salsabila Qisthina Yasmine, Marlina Azzahra, Alviska Rizkikha Hafsyari, Debriyanti Lydia Charismata, Zalfa Nurul Zahirah Biology Study Program, Universitas Negeri Jakarta Abstract Currently as many as 35,000 microalgae have been identified in the world. One of the identification of microalgae diversity can be found in the Bogor Botanical Gardens (KRB). The purpose of this study was to determine the diversity index at the microalgae class level and the microalgae diversity index at each station in the Bogor Botanical Gardens. This research was also carried out to update information on the diversity of microalgae in the Bogor Botanical Gardens which will be useful in maintaining and managing ponds in the Bogor Botanical Gardens tourist area. This research was conducted at the Bogor Botanical Gardens, then observations and identification were carried out at the Zoology Laboratory, Univeritas Negeri Jakarta. The research sampling method used purposive sampling at 8 stations with 2 replications. Water samples were taken using a 10 liter bucket at the periphery of the pond and flowed into the plankton net. The samples were then prepared with the addition of 10% Lugol^s iodine. Abiotic factors such as temperature, pH, and light intensity were also measured as data. Then, the diversity index analysis was carried out using the Shannon-Wiener index equation. The results showed that the diversity of the microalgae class was in the medium category, with the highest diversity index of the microalgae class being in the Bacillariophyceae class (2.29). Meanwhile, the microalgae diversity index number included in the medium category was shown at station 1 (2.5) and the lowest microalgae diversity index number was shown at station 8 (0.97). Keywords: Microalgae, diversity, Bogor Botanical Gardens Share Link | Plain Format | Corresponding Author (Rizky Priambodo)

43	Biology	ABS-207
Gamma Iradiation Effect on Growth and Development of Foxtail Millet (Setaria italica (L.) Buru Merah and Polman Kuning Accession Rizky Priambodo, Reni Indrayanti, Adisyahputra, Ivana Bertha Sabella Biology Study Program, Universitas Negeri Jakarta Abstract Foxtail millet (Setaria italica (L.) P. Beauv is a cereal plant originating from China and has been cultivating in several parts of Asia. The use of foxtail millet in Indonesia is not optimal due to its small seed size and low yields. One way to overcome this problem is by increasing the genetic potential of seed quality commodities through mutation induction. The purpose of this study was to determine the effect of gamma irradiation on the phenotypic characters of Polman Kuning and Buru Merah and the optimum dose to increase the genetic diversity of Polman Kuning and Buru Merah accessions. This research was conducted at the Greenhouse, Jakarta State University Biology Study Program and took place from September to December 2020. The method used was an experimental method using a completely randomized design (CRD) with two factors, namely the gamma irradiation dose (0, 25, 50, 75, 100, 125 Gy) and two accessions of foxtail millet (Polman Kuning and Buru Merah). The results showed that irradiation (0, 25, 50, 75, 100, 125 Gy) caused 50% plant mortality at a dose of 99 Gy on Buru Merah and 127 Gy on Polman Kuning. Buru Merah accessions treated with irradiation at a dose of 50 Gy were more effective in inducing plant height, leaf length, leaf width, leaf number, and stomata density. Polman Kuning accession treated with irradiation at a dose of 25 Gy was more effective in inducing plant height, leaf length, leaf width, the number of leaves, and stomata density. Irradiation treatment of Buru Merah and Polman Kuning plants did not show any difference in their phenotypic characters. The application of gamma-ray irradiation to optimize the seed weight of Buru Merah accession was the treatment dose of 50 Gy, while to optimize the seed weight of Polman Kuning was the treatment dose of 25 Gy. Keywords: foxtail millet, induced mutation, gamma irradiation Share Link | Plain Format | Corresponding Author (Rizky Priambodo)

44	Biology	ABS-213
Fabrication and Characterization of Cellulose Acetate Nanofiber Derived from Rice Husk as a Potential Wound Dressing Material Aryanny Nasir, Saiful Izwan Bin Abd Razak and Siti Pauliena Mohd Bohari Department of Bioscience, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, 81310, Johor, Malaysia School of Biomedical Engineering and Health Sciences, Faculty of Engineering, Universiti Teknologi Malaysia, Johor Bahru, 81310, Johor, Malaysia. Cosmetic and Fragrance Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia. Abstract Electrospun nanofibrous mat have been fabricated from a variety of natural and synthetic polymers and ideal for wound dressing applications. Due to their appealing characteristics, natural polymers have attracted a lot of attention. Hence, the use of cellulose derived from agricultural waste becomes crucial as a green alternative to produce electrospun wound dressing. The feasibility of preparing cellulose acetate (CA) nanofiber derived from rice husk (RH) through electrospinning process and investigating its wettability for potential application as wound dressing was reported. Different CA concentrations of 15 percent, 16 percent and 20 percent (w/v) were fabricated and labelled as CA15, CA16, and CA20 respectively. The morphology and wettability of the prepared CA nanofiber was studied using scanning electron microscopy and static contact angle measurements. The scanning electron microscope (SEM) images showed continuous fiber with diameter ranging from 100-600 nm with good adhering of the human dermal fibroblast (HDF) cells onto the CA nanofiber. The results also showed that the electrospun membrane gave a contact angle of 127.2o, 125.8o, 108.6o for CA15, CA16, and CA20, respectively. Higher concentrations of CA resulted in bead-free fibres with higher porosity. CA20 has excellent porosity of 76.09\pm1.57, which is essential for scaffoldings. Based on the findings, it could be concluded that the fabricated electrospun mat derived from RH could be potentially applied as wound dressing for successful wound healing. Keywords: electrospun, nanofiber, cellulose, rice husk, wound dressing Share Link | Plain Format | Corresponding Author (Aryanny Binti Nasir)

45	Biology	ABS-214
Orthosiphon aristatus ethanolic extract accelerate wound healing process via in vitro study Hafedh Ahmed Al-moalemi, Chua Lee Suan, Siti Pauliena Mohd Bohari a) Department of Bioscience, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia b) N22, Institute of Bioproduct Development, University Technology Malaysia, 81310, Skudai, Johor, Malaysia c) Cosmetic and Fragrance Laboratory, Institute of Bioproduct Development, Universiti Teknologi Malaysia. Abstract Orthosiphon aristatus is a herbal plant from the family of Lamiaceae. The plant especially the leaves is widely used to treat diseases such as diabetes mellitus, urinary disorders, rheumatism and hypertension. Additionally, numerous pharmacological research has shown that this plant has the ability to exhibit antioxidant, anti-inflammatory, and antibacterial activity. However, there are not enough studies supporting its role in wound healing mechanisms. Therefore, the purpose of this study was to investigate the wound healing activity of O. aristatus ethanolic extract toward human skin fibroblast cells HSF using the migration assay. Our preliminary study indicated that the IC50 value of the O. aristatus ethanolic extract from the cytotoxicity study was 512.8 microg per ml. Furthermore, the extract of O. aristatus at a concentration of 20 microg per ml, which was lower than the IC50 value, had successfully enhanced wound closure by 93.6 percent within 18 hours of incubation compared to the control group 89.1 percent. Based on the findings, further study using an animal model is required to enlighten the proper mechanism of this plant^s ability to be employed as a wound-healing regime. Keywords: Orthosiphon aristatus- Wound healing- Cell viability Share Link | Plain Format | Corresponding Author (Hafedh Ahmed Al-moalemi)

46	Biology Education	ABS-6
EFFECT OF VIRTUAL FIELD TRIP INSTRUCTIONAL STRATEGY ON ACQUISITION SCIENCE PROCESS SKILLS AND PERFORMANCE AMONG SCIENCE EDUCATION STUDENTS IN KUST DR. SAUDAT SHEHU BALA and DR. SURAJO ISA GAYA KANO UNIVERSITY OF SCIENCE AND TECHNOLOGY EDUCATION, WUDIL, KANO STATE, NIGERIA Abstract The research is intended to find the Effect of Virtual Field Trip Instructional Strategy on Performance and Science Process Skills Acquisition among Science Education Students in Kano University of Science and Technology, Wudil. Two objectives, two research questions and two null hypotheses were formulated. The population of the study is made up of all the science education students in Kano University of Science and Technology, Wudil. Quasi experimental research design was adopted for the purpose of the study- employing two groups (experimental group and control group). The instruments used for data collection are Science Education Performance Test (SEPT) and Science Process Skills Acquisition Test Questionnaire (SPSATQ). The sample of this study is composed of four subject combinations. The sample size is made up of seventy seven (77) students for the experimental group and seventy four (74) students for the control group. The data was analyzed using t-test for testing the stated hypotheses at 0.05% level of significance. The result showed that virtual field trip instructional strategy improved performance as well as the acquisition of science process skills among science education students in KUST, Wudil. It is therefore recommended that workshops, seminars and conferences should be organized for science education lecturers on the use of virtual field trip instructional strategy. In conclusion the study has shown that virtual field trip Instructional Strategy is an effective teaching technique in science education classrooms. Keywords: Virtual Field Trip, Performance, Science Process Skills Introduction Share Link | Plain Format | Corresponding Author (Saudat Shehu Bala)

47	Biology Education	ABS-10
DEVELOPMENT AND VALIDATION OF TPACK-BASED MICROTEACHING ASSESSMENT INSTRUMENTS Eka Putri Azrai (a), Daniar Setyo Rini (a*), Ade Suryanda (a) a) Biology Education Study Program, Universitas Negeri Jakarta Jalan Rawamangun Muka, Jakarta, Indonesia *daniarsetyorini[at]unj.ac.id Abstract Microteaching is a teaching method currently used by many universities in Indonesia to provide real-world experiences and practice basic teaching skills. Microteaching can provide optimal results if accompanied by observation, assessment, and optimal feedback to students. Observation, assessment, and feedback activities will run well if adequate assessment instruments support them, meet the needs of the times, and are valid. This study aims to develop and validate an instrument for assessing basic teaching skills in implementing microteaching. The instrument was developed by adding skills in using technology in TPACK-based learning following the demands of teacher competence in the 21st century. This research is development research. The sample involved in this study was 53 students. The instrument that has been developed can be used for the implementation of microteaching either online or offline. A total of 78 statement items were generated to assess eight basic teaching skills. Three validators carried out expert validation and stated that 92.4% of the instrument was feasible to use. The reliability test with Cronbach^s alpha is 0.9 (very reliable). The item validity test resulted in 56 valid items, and for the evaluation survey, student responses as instrument users gave good results. Keywords: Microteaching, Research and Development, TPACK Share Link | Plain Format | Corresponding Author (Daniar Setyo Rini)

48	Biology Education	ABS-12
PERSEPSI DAN MOTIVASI BELAJAR BIOLOGI SISWA SELAMA KEGIATAN BELAJAR DARI RUMAH Ade Suryanda,1* Eka Putri Azrai,1 Nurlaela Widyasari1 1Pendidikan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Jakarta Abstract Masa darurat penyebaran Covid 19 di Indonesia membuat pemerintah menerapkan kebijakan pendidikan yakni pelaksanaan kegiatan belajar dari rumah. Kebijakan ini membuat siswa harus beradaptasi agar kegiatan belajar tetap berjalan optimal. Dalam proses belajar biologi, motivasi sangat dibutuhkan untuk mencapai tujuan pembelajaran. Motivasi belajar dipengaruhi oleh beberapa faktor, salah satunya adalah persepsi siswa terhadap kegiatan belajar dari rumah. Penelitian ini bertujuan untuk mengetahui hubungan antara persepsi siswa terhadap kegiatan belajar dari rumah dengan motivasi belajar siswa pada mata pelajaran biologi. Penelitian ini menggunakan metode deskriptif kuantitatif dengan studi korelasional. Populasi penelitian ini adalah Siswa SMA Negeri 48 Jakarta dengan jumlah sampel sebanyak 125 siswa kelas XI MIPA yang dipilih melalui teknik Simple Random Sampling. Hasil analisis menunjukkan terdapat hubungan positif antara persepsi siswa terhadap kegiatan belajar dari rumah dengan motivasi belajar siswa pada mata pelajaran biologi. Tingkat hubungan termasuk dalam kriteria rendah, oleh karena itu untuk meningkatkan motivasi belajar siswa tidak hanya dengan meningkatkan persepsi siswa melainkan banyak faktor lain yang mempengaruhi, karena motivasi belajar dapat timbul dari dalam diri individu (intrinsik) dan dari luar individu (ekstrinsik). Keywords: belajar dari rumah, covid 19, motivasi belajar, pandemi, persepsi siswa Share Link | Plain Format | Corresponding Author (Nurlaela Widyasari)

49	Biology Education	ABS-15
Exploration Experiential Model Affects Science Process Skills (SPS): Student^s Plant Identification Ability Increases? Diana Vivanti Sigit1(a*), Ratna Dewi Wulaningsih(b), Alma Tasya Yuanisa(b) (a*)State University of Jakarta , Jakarta (b) State University of Jakarta , Jakarta (c) State University of Jakarta , Jakarta Abstract Science process skills (SPS) are skills that need to be developed in biology learning. The application of learning models that are less varied in each lesson causes student boredom, so students can only observe learning objects through pictures or videos. Exploration experiential model is a model that can build student learning experiences through exploration of the natural surroundings. This research aims to determine the effect of the exploration experiential model on students^ SPS in identifying plants in spermatophyte submaterial compare with the discovery learning model. This research was conducted in the even semester of the 2021/2022 academic year at SMA Negeri 18 Bekasi. The method used is quantitative quasi-experimental and pretest-posttest non-equivalent control-group-design, and the sample is 58 students. The results of the t-test showed that there was a significant difference between the exploration experiential and discovery learning models toward the SPS. Futhermore, the exploration experiential model had an effect on students^ SPS in identifying plants. This was evidenced by the increase in students^ SPS in the experimental class which were categorized as highly skilled, while less skilled in the control class. Thus, exploration experiential model can be used as a reference to improve students^ SPS in learning biology. Keywords: exploration experiential model, identifying plants, science process skills (SPS), spermatophyte. Share Link | Plain Format | Corresponding Author (Alma Tasya Yuanisa)

50	Biology Education	ABS-16
The Relationship Between Immune System Knowledge and Attitude of Using Herbs as Immunomodulator among High School Students Yulilina Retno Dewahrani, Rusdi, Sheilla Safitri, Budi Rahayu Universitas Negeri Jakarta, Indonesia Public Senior High School 61 Jakarta, Indonesia Abstract Knowledge about immune system is an important thing to have in order to maintain health and increase body immunity. Knowledge about immune system taught to students not only serves as information, but is expected to promote positive attitudes, one of which is the attitude of using herbs as immunomodulators. This is because knowledge has an important role in the formation of an attitude in an individual. It is important for high school students to have a positive attitude in using herbs so that they can improve their immune system and avoid various diseases. This study aims to determine the relationship between knowledge about immune system with the attitude of using herbs as immunomodulators in high school students. This research method is correlational descriptive. The independent variable in this study is knowledge about immune system and the dependent variable in this study is the attitude of using herbs as immunomodulators. Sampling was done by purposive sampling technique and continued with simple random sampling until 80 respondents were obtained. Testing the correlation coefficient of the two variables was carried out using the Pearson Product Moment formula. Based on the calculations obtained a significance value of 0.014 < 0.05 and a correlation coefficient of 0.274. It can be concluded that there is a weak and positive relationship between knowledge about immune system and the attitude of using herbs. Keywords: Attitude, Herbs Use, Immune System, Immunomodulator, Knowledge Share Link | Plain Format | Corresponding Author (Sheilla Safitri)

51	Biology Education	ABS-19
The effect of Augmented Reality Respiratory System (AR Sinaps) Learning Media on High School Students^ Biology Learning Outcomes Daniar Setyo Rini, Eka Putri Azrai, Anidya Annisa Khansa Bachelor Degree of Biology Education Study Program, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia Abstract Learning outcomes are the grades achieved by a student in a class. The current condition shows the low student learning outcomes, especially in subjects that require a high understanding of concepts such as biology, one of which is the respiratory system material. Therefore, students need innovative, engaging, interactive, and fun media that can help them understand biological material by visualizing the shape, structure, and location of the organs that make up the body system. This study aims to determine the effect of the application of learning media augmented reality respiratory system (AR Sinaps) in learning biology on the learning outcomes of high school students. This research was conducted in February-March at SMA Negeri 18 Bekasi. This research method is a quasi-experiment with a posttest-only control group design. The sample of this study amounted to 60 students, with each class totaling 30 students. The data analysis technique used is the Mann-Whitney test with a significant level (&#120572-) = 0.05. The average value of learning outcomes obtained by the experimental class is 90.60, and the control class is 76.07. Based on the Mann-Whitney test, this research showed the effect of Augmented Reality Respiratory System (AR Sinaps) Learning Media significantly on High school students^ learning outcomes. Keywords: augmented reality, learning media, learning outcomes, respiratory system Share Link | Plain Format | Corresponding Author (anidya khansa)

52	Biology Education	ABS-33
Learning Loss Analysis on Science Literacy for Elementary School Students in the Covid-19 Pandemic Ika Lestari (a*), Yurniwati (a), Imaningtyas (a), Arita Marini (a), Diah Merrita (b) a) Universitas Negeri Jakarta Jalan Rawamangun Muka, Jakarta Timur, 13220, Indonesia *ikalestari[at]unj.ac.id b) Sekolah Tinggi Bahasa Asing, LIA Jl. Pengadegan Timur Raya No.3, RT.5/RW.2, Pengadegan, Kec. Pancoran, Kota Jakarta Selatan, Daerah Khusus Ibukota Jakarta 12770 Abstract The Covid-19 pandemic has caused learning loss at all levels of education, including scientific literacy in the elementary school level. The purpose of this study was to identify and analyze scientific literacy indicators (from Sasseron & Carvalho (2008), Bybee (1997)) which were in the undeveloped category when learning loss occurred. The research method used is descriptive quantitative. The research sample was taken using simple random sampling technique. Data was collected by distributing scientific literacy tests and questionnaires to students for fourth grade in two schools, namely the Karawaci Baru 3 Public Elementary School Tangerang and the Al-Kautsar Integrated Islamic Elementary School, Cikarang as many as 65 students. Then, the data is analyzed using Descriptive Statistics with reference to the categorization norm. The results showed that the scientific literacy ability of elementary school students was still not developed because the learning model used by the teacher during distance learning was limited to the mastery of technology by the teacher, the participation of parents, and the infrastructure facilities owned by the students. The conclusion of this study is that the acquisition of scientific literacy skills in students is also influenced by the uniqueness of the school in internalizing science values in learning and learning activities when distance learning is carried out. The results of this study can be recommended for further research in developing a learning model that is able to overcome learning loss in scientific literacy, one of which is through an integrated mobile learning system. Keywords: learning loss, science literacy, elementary school students Share Link | Plain Format | Corresponding Author (Ika Lestari)

53	Biology Education	ABS-35
STUDENTS^ PERCEPTION CONCERNING E-LEARNING BASED ON MOODLE PLATFORM: A STUDY IN HIGHER EDUCATION Lesy Luzyawati(a*), Ali Miftakhu Rosyad(b), Lissa(a) a) Biology Educational Department, Universitas Wiralodra, b) Islamic Education Department, Universitas Wiralodra, Indonesia Jl. Ir. H. Juanda Km. 3, Indramayu, West Java, Indonesia *lesy.luzyawati[at]unwir.ac.id Abstract Online learning (e-learning) has become an alternative method teaching and learning in higher education. Many applications used on virtual learning, one of them is Moodle. This study aims to investigate the perception of students to carry out e-learning based on Moodle platform especially in higher education. The study conducted quantitative and qualitative approach with a survey method using questionnaire among the undergraduate students. The result showed that the students expressed in high category to respond Moodle platform as e-learning. It was successfully implementing of e-learning because half of the students^ agreed 47 percent, strongly agreed 6 percent, doubtful 35 percent, and disagreed 13 percent. The Moodle platform also could increase student learning outcomes with medium category. The implication is positive perception of student is needed to enhance learning transformation. Keywords: students^ perception- Moodle platform- virtual classroom- learning outcome Share Link | Plain Format | Corresponding Author (Lesy Luzyawati)

54	Biology Education	ABS-55
Design of Conceptual Framework on Integrated Mobile Learning System in Developing Science Literature Elementary School Students Ika Lestari (a*), Diah Merrita (b), Yurniwati (a), Arita Marini (a) a) Jl. Rawamangun Muka Raya No.11, RT.11/RW.14, Rawamangun, Kec. Pulo Gadung, Kota Jakarta Timur, Daerah Khusus Ibukota Jakarta 13220 *ikalestari[at]unj.ac.id b) Sekolah Tinggi Bahasa Asing LIA Jl. Pengadegan Timur Raya No.3, RT.5/RW.2, Pengadegan, Kec. Pancoran, Kota Jakarta Selatan, Daerah Khusus Ibukota Jakarta 12770 Abstract Scientific literacy in elementary school students shows undeveloped results, this is expanded by the covid-19 pandemic which causes loss learning. The purpose of this study was to design a conceptual framework for an integrated mobile learning system (combining formal and informal learning) in developing scientific literacy in elementary school students to excel in the covid-19 pandemic. The research method is descriptive qualitative with data collecting techniques such as interviews to material experts, media, and instructional design as validation of the conceptual framework that has been made. The time of the research was carried out during 2022. The results show that the integrated mobile learning system has a theoretical basis, namely learning design for media (combining formal and informal learning), elementary school student development, and scientific literacy that are made in one comprehensive framework so that it can be used as base in the manufacture of an integrated mobile learning system. The next research recommendation is that the results of the conceptual framework can be developed into an integrated mobile learning system to be tested on elementary school students related to scientific literacy. Keywords: conceptual framework, integrated mobile learning system, scientific literacy, formal learning, informal learning Share Link | Plain Format | Corresponding Author (Ika Lestari)

55	Biology Education	ABS-62
Analysis of Gastropods Abundance and The Relationship with Mangrove Density in Pulau Rambut Wildlife Reserve Ade Suryanda1, a), Eka Putri Azrai1, b), Avarha Mahaputri1, c), Agung Satiya1), Annisa Fitriani1), Cynthia Loka1), Khafifah Nurhasanah1), Warsa Jaya2) 1) Program Studi Pendidikan Matematika, FMIPA, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta Timur 13220, Indonesia a) asuryanda[at]unj.ac.id, b) ekaputri[at]unj.ac.id, c) avarhamahaputri[at]gmail.com 2) Balai Konservasi Sumber Daya Alam DKI Jakarta, Indonesia Abstract The mangrove forest ecosystem has a significant contribution to the biota that live in the surrounding waters as a food source, one of which is the gastropod class. The purpose of this study is to determine mangrove density, gastropod abundance, and the correlation between mangrove density and gastropod abundance. This research was conducted in March to June 2022 in the Pulau Rambut Wildlife Reserve. The method used in this research is a survey method in the field by drawing a line 50 meters long, then dividing the line into 3 plots, measuring 10 x 10 meters with a distance between plots of 10 meters. In order to determine the sampling point to take gastropods is by using purposive sampling technique. The data is categorized based on the variables. Mangrove density as the variable X, and the abundance of gastropods as the variable Y. The results showed that the mangrove density in the North, East, and Southwest of the Pulau Rambut Wildlife Reserve was included in the very good category with a value of 33,400 ind/ha. The highest abundance of gastropods was dominated by the species Aclis sp. with a total of 4,089 individuals and the lowest was Monodonta sp. totaling 6 individuals. Mangrove substrate and organic matter content in the area determine the abundance of gastropods. The correlation between mangrove density and gastropod abundance was calculated using simple linear regression analysis with correlation coefficient of 0.148, while the value of a determination coefficient was 0.022 which means it is positively correlated. Keywords: Correlation- gastropods abundance- mangroves density- Pulau Rambut Wildlife Reserve. Share Link | Plain Format | Corresponding Author (Avarha Mahaputri Jasmine Novarman)

56	Biology Education	ABS-66
Tourists^ Attitudes towards Conservation Efforts on the Sepanjang Beach Yogyakarta Diana Vivanti Sigit, Mieke Miarsyah, Ana Syafrotul Karomah, Felia Nurjihan Pratiska, Indah Praminingsih Magister Pendidikan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Jakarta. Abstract Indonesia has an abundant diversity of biological resources. This can affect the tourism sector which is an attraction for tourists to visit coastal areas in Indonesia, both domestic and foreign tourists. Therefore, the contribution of the community and tourists is needed in an effort to maintain and carry out conservation in the coastal ecosystem area. The involvement of the community and tourists in conservation efforts is expected to increase a sense of responsibility so that environmental care is maintained. This study aims to describe the attitudes of tourists, identify the attitudes of tourists, and determine the participation of tourists in conservation efforts at Pantai Panjang, Yogyakarta. The type of research carried out is descriptive research with survey methods, through filling out validated questionnaires and literature studies. The sample in this study amounted to 50 tourists who were in the area of Panjang Beach, Yogyakarta with accidental sampling technique. The data analysis technique used is descriptive analysis to analyze tourist attitudes towards conservation efforts. Based on the results obtained, it is known that most of the tourists on the Panjang Beach, Yogyakarta meet the attitude criteria that are seen based on the dimensions of cognition, affection, and conation in conservation efforts. The attitude of tourists as a whole is included in the very good criteria. Keywords: attitudes, conservation, sepanjang beach, tourists. Share Link | Plain Format | Corresponding Author (Indah Praminingsih)

57	Biology Education	ABS-74
THE DEVELOPMENT OF WEB-BASED AUGMENTED REALITY (AR) TECHNOLOGY AS A LEARNING MEDIA ON THE NERVOUS SYSTEM MATERIAL Refirman Djamahar2, Mahrawi Mahrawi1, Ninda Ayu Ningtiyas1, Rizhal Hendi Ristanto2, Mila Ermila Hendriyani1, Rida Oktorida Khastini1, Choirul Huda3, Yulilina Retno Dewahrani2 1Biology Education Department, Faculty of Teacher Training and Education, Sultan Ageng Tirtayasa University, Cilegon/Serang, Indonesia 2 Biology Education Department, Faculty of Mathemathic and Science, State University of Jakarta, DKI Jakarta, Indonesia 3Industrial Engineering Department, Faculty of Engineering, Serang Raya University, Serang, Indonesia *) Corresponding Authors: mahrawi[at]untirta.ac.id.1, ninda.ayuningtiyas[at]gmail.com.1 Abstract This research aimed to develop a web-based AR learning media on the nervous system subject, as well as to determine the feasibility of media from experts and samples. This research is a development research (R&D). Research samples were 20 students of 11th grader of science class. The process of SarafAR development is done by analyzed various problems and potential innovations obtained from schools and literature studies, collected initial data to support the development process, designed media products by built a system architecture designs, storyboards, and visualized system designs. Applications used include Blender, Sketchfab.com, Microsoft PowerPoint, WebAR Zappar, and Wixsite.com. The results of the expert test analysis showed that the mean value of the material expert team was 84.2% and the media expert team was 88.3% as a very good media. as a very good media. The results from the student response test obtained a media feasibility interpretation score from the use of the SUS instrument of 81.3 so that it met the scoring interpretation criteria of more than 80.3 which means that SarafAR is a good learning media and well accepted to be used by students and to help students understand the nervous system subject. Keywords: augmented reality (ar), web-based media, nervous system subject Share Link | Plain Format | Corresponding Author (Refirman Djamahar)

58	Biology Education	ABS-75
The Critical Thinking Skill of High School Students on The Topics of Virus and Bacteria Yenny Anwar1, Adeng Slamet2, Niara Gustini3 Universitas Sriwijaya Abstract This study aims to determine the critical thinking skills of students on viruses and bacteria in high school, Muara Enim sub-district. This is a descriptive study with a survey method. The sample was determined using a cluster sampling technique followed by a stratified random sampling technique to determine the number of samples for each school. This research was conducted at four high school in Muara Enim District. The results showed that the critical thinking skills of were mostly categorized as very low. This can be seen from the percentage values, namely the high category of 2.46% of students, medium category of 4.93% of students, low category of 27.59 students, and very low 65.02% of students. The highest critical thinking ability indicator is the assumption of 51.48%, while the lowest is in interpretation and drawing conclusions with a percentage of 24.96%. The achievement of test results that are categorized as very low is evident in the questionnaire statement item responses to students^ perceptions of critical thinking skills in the statement of ability to solve questions where only 32.51% of students do not experience difficulties in answering questions. Keywords: Critical Thinking Skills, Viruses and Bacteria. Share Link | Plain Format | Corresponding Author (Yenny Anwar)

59	Biology Education	ABS-86
Development of Digital Comic based on Biology Literacy as Learning Media in the Topic of Immune System Yulilina Retno D., Nurmasari Sartono, Halimah Syifa F. Faculty of Mathematics and Natural Sciences, Jakarta State University, Jalan Rawamangun Muka, Jakarta 13320, Indonesia Abstract The development of technology in 21st century demands students to own specific skills, one of which is biology literacy. Unfortunately, students scientific literacy rate in Indonesia is still categorized as low. One of the ways to increase biology literacy is to utilize appropriate learning media for the learning process. Based on the explanation before, the aim of this study is to develop digital comic based on biology literacy as learning media on the topic of immune system. The development model used in this research is Borg & Gall model which modified into seven steps. The product went through validity test on media, concept and languange aspects by two validator on each aspect. Validity test resulted as the following- 93,88% on media aspect, 90% on concept aspect, and 93% on languange aspect, which indicates digital comic based on biology literacy is valid to be tested on a larger scale. Small group trial test then conducted on 11th grade students of SMAN 4 Bekasi. Test results showed overall score of 90%, which indicates that digital comic based on biology literacy can be used as a valid learning media in the topic of immune systems. Keywords: Development research, Learning media, Digital Comic, Biology Literacy Share Link | Plain Format | Corresponding Author (Halimah Syifa Febrina)

60	Biology Education	ABS-87
Sign Vocabulary to Support Biology Education for Deaf And Hard Of Hearing Students Dra. Yulilina Retno Dewahari, M.Biomed, Dr. Mieke Miarsyah, M.Si, Denada Kinanti Mahapranawati (*) Faculty of Mathematics and Science, Jakarta State University, Jl. R.Mangun Muka Raya No.11, Jakarta, Indonesia, 13220 * denadakinanti[at]gmail.com Abstract One of the Sustainability Development Goals (SDGs) target is Quality Education which emphasize in quality education as well as ensure fair and inclusive education. The COVID-19 pandemic hinders the achievement of SDGs targets. One of the most vulnerable groups to be affected by the pandemic is deaf and Hard of Hearing (HoH) people, who had difficulty communicating since online learning was applied. The purpose of this study was to develop sign vocabulary to support Biology education for deaf and HoH students. This research and development (R&D) study used ADDIE Model. There are 5 steps in ADDIE Model, which includes Analyze, Design, Development, Implementation, and Evaluation. The final product of this study is a demonstration video containing sign vocabulary to support Biology education. This study was conducted from March to July 2022. The developed sign vocabulary^s eligibility was tested by media and material experts. The test resulted in 78% percentage of media eligibility and 79% percentage of material eligibility, both aspects interpretated that the vocabulary is eligible to be used. Further test was conducted on Science teacher and grade X students of SMALB Negeri 1 Jakarta. The overall percentage score of the developed sign vocabulary for deaf and HoH students was 78.5%. Based on the results from the media and material eligibility test as well as from the respondent testimonies, it can be concluded that the developed sign vocabulary is valid and eligible to be used in biology learning process for deaf and HoH students. Keywords: Sign Vocabulary- deaf and Hard of Hearing (HoH)- Research and Development- Biology Education Share Link | Plain Format | Corresponding Author (Denada Kinanti Mahapranawati)

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