Effect of 6-Benzylaminopurine and Repeated Subculturing for in vitro Shoots Production of Banana (Musa acuminata x balbisiana, ABB) Reni Indrayanti,Sunani, Agus Sutanto, Sudarsono, Adisyahputra
Universitas Negeri Jakarta
Abstract
Banana cv. Kepok (Musa acuminata x balbisiana-ABB) is one of the supporting plants for food security. The production of banana cv. Kepok can be achieved through in vitro culture techniques by increasing the concentration of plant growth regulators in repeated subcultures. The study aims to determine the effect of repeated subcultures with the increasing addition of BAP to produce in vitro banana cv. Kepok clones. This research uses an experimental method with a Completely Randomized Design (CRD). Banana aseptic shoots were grown in Murashige and Skoog media containing 0.175 mg L-1 IAA and different concentrations of BAP. The experiment consisted of 3 BAP treatments with concentrations of 4.50 mg L-1 BAP (I1B1), 6.75 mg L-1 BAP ((I1B2), and 9.00 mgL-1 BAP (I1B3). Each stage of BAP was subcultured 1-2 times with six subcultures for six months. The independent T-test results showed a significant effect on the average number of shoots, leaves, and roots of the Kepok cultivar after being subcultured in stages for six months (p<0.05). The number of shoots per explant after multiplication and subculturing to the Murashige and Skoog media supplemented with 0.175 mg L-1 IAA, and increasing concentration of BAP shows that the average number of shoots increased from 3.5 shoots per explant and became 13.9 shoots per explant. The avarage number of leaves increased from 6.2 leaves per explant and became 14 leaves per explant. In addition, the number of roots increased from 0.4 roots per explant became 6.26 roots per explant. This study showed that subculturing banana explants in Murashige and Skoog medium containing BAP with increasing concentration for six months produced 644 explants of banana, which is increased 43fold from initial explants with the average number of shoots per explant being 13.9 shoots. This study concludes that BAP and repeated subculturing can produce in vitro banana shoots.
Keywords: Indole 3-acetic acid, genom ABB, Kepok Cultivar, mass production, Murashige and Skoog.
