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61	Aquatic Pollution and Ecotoxicology	ABS-56
Karakteristik mikroplastik pada organ ikan Argyrosomus japonicus (Awon awon) dan Scomber australasicus (Selengseng) yang didaratkan di PPN Prigi, Jawa Timur Syarifah Hikmah Julinda Sari(a)-Defri Yona(a*)- Ledhyane Ika Harlyan(a)- Mochammad Arif Zainul Fuad(a)- dan Yuniar Ponco Prananto(b) (a)Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya *defri.yona[at]ub.ac.id (b)Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Brawijaya Abstract Keberadaan mikroplastik memiliki sifat yang sulit terdegradasi berpotensi untuk terakumulasi di berbagai organ ikan. Penelitian ini bertujuan mengevaluasi karakteristik mikroplastik yang ditemukan di organ ikan Argyrosomus japonicus (Awon awon) dan Scomber australasicus (Selengseng) yang didaratkan di PPN Prigi, Jawa Timur. Sebanyak 10 ikan Awon awon dan Selengseng dipilih sebagai sampel, kemudian mikroplastik pada organ insang, saluran pencernaan dan daging diektraksi dengan larutan H2O2 dan Fe(II). Hasil penelitian menunjukkan jenis fiber dan fragmen ditemukan pada kedua ikan dimana jenis fiber lebih tinggi daripada fragmen. Kelimpahan fiber tertinggi di daging, diikuti oleh insang dan paling kecil di saluran pencernaan di ikan Awon awon. Pada ikan Selengseng, kelimpahan mikroplastik jenis fiber tertinggi ditemukan pada insang, daging dan saluran. Penelitian ini menemukan keberadaan mikroplastik jenis fiber di daging ikan, sehingga penelitian lanjutan tentang dampak konsumsi ikan yang mengandung mikroplastik pada manusia perlu dilakukan. Keywords: mikroplastik-ikan komersial-organ-fiber Share Link | Plain Format | Corresponding Author (Syarifah Hikmah Julinda Sari)

62	Aquatic Pollution and Ecotoxicology	ABS-65
SEPARATION, IDENTIFICATION AND CHARACTERIZATION OF MICROPLASTICS FOUND IN THE SURFACE WATER OF LANGAT RIVER, MALAYSIA Ahmad Ammarluddin Ali (a*), Sabiqah Tuan Anuar (b), Mohd Kalkausar Ku Yusof (b), Wan Mohd Afiq Wan Mohd Khalik (b), Maisarah Jaafar (b), Yusof Shuaib Ibrahim (b) a) Faculty, Department of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. b) Microplastics Research Interest Group (MRIG), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia. Abstract Microplastics (MPs) are found across the world, including in aquatic habitats. While several studies on MPs have been conducted in marine environments, there are relatively few investigations in freshwater habitats. In this research, the occurrence of MPs on surface water from Langat River, Malaysia, was investigated. The water surface was collected at six different points: coastal, estuary, and freshwater. Samples were analysed physically, chemically and statistically. Study found the abundance of MPs was 1820 \pm 1320 items/L and were majority by small in size (0.1-0.01 mm), transparent in color and fragment in shape. Other than that, the surface texture and elemental composition of MPs including cracks, pit holes, and texture and adherence particles were examined using a Scanning Electron Microscope (SEM). Fourier transform infrared (FTIR) has corroborated more than 80% polymeric composition of polyethylene and polypropylene for large microplastics (LMPs). Principal component analysis (PCA) concludes turbidity has a significant effect on the MPs abundance due to the urbanization and high population density. Keywords: Langat, Malaysia, microplastic, surface water, turbidity Share Link | Plain Format | Corresponding Author (Ammarluddin Ali)

63	Aquatic Pollution and Ecotoxicology	ABS-72
Analytical Pyrolysis for monitoring and characterization of microplastics in environmental matrices Sabiqah Tuan Anuar, Ammarluddin Ali, Nurul Izzati Abul Razak, Yusof Shuaib Ibrahim, Wan Mohd Afiq Wan Mohd Khalik, Ku Mohd Kalkausar Ku Yusof, Maisarah Jaafar, Lee Bai Qin, Chin Teen Teen Microplastic Research Interest Group (MRIG), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Abstract The increasing human population has made the demands for resources and gathering areas which resulted in expansion of terrestrial urban areas, thus now intruding the coastal territory. The introduction of municipal waste, and unregulated management of trash not only brought ecological changes, but also, negatively affect many organisms living therein especially through pollution and food scarcity. Microplastics, at sizes less than 5 mm, can affect the further up the food web through bioaccumulation, bioavailability, and biomagnification. Traditional qualitative method for microplastics analysis alone is inadequate without precise polymer identification. This study emphasized the use of the thermal degradation method of pyrolysis-GC/MS as characterization technique to identify the type and concentration of microplastic in analytical volume of sample matrices. Pyrolysis-GC/MS measurement were carried out at 600 oC (pyrolyzer temperature) and an optimized selective ion monitoring (SIM) mode for MS was used to analyze selected polymer standards. Individual standard calibration was performed before the method was applied to real sample matrices. The samples of environmental matrices (water and biota) were collected around the east coast of peninsular Malaysia (South China Sea) and were subjected to digestion and extraction processes using a wet peroxidation method before microplastics were physically observed and analyzed. A detail thermal degradation profile using an optimized pyrolysis-GC/MS method indicates that pyrolytic products for polypropylene, polyethylene, PET, Nylon, PMMA and chlorinated polyethylene were successfully detected. The technique gives additional information about the chemical constituent and suggested that the sample can be coming from complex polymer component or different polymer materials in one single sample. This study provides an insight of the level of microplastic pollution in biota samples found in Malaysia and discusses the need of appropriate characterization methods for complex sample matrix. Keywords: microplastics, plastic pollution, Malaysia, fish, polychaete, surface water Share Link | Plain Format | Corresponding Author (Sabiqah T. Anuar)

64	Aquatic Pollution and Ecotoxicology	ABS-75
The occurrence and distribution of microplastics in Malaysia riverine systems utilizing SEM EDS, micro FTIR and pyrolysis GC/MS Yusof Shuaib Ibrahim (a), Sabiqah Tuan Anuar (a), Nor Salmi Abdullah (b), Nasehir Khan E.M Yahya (b), Teen Teen Chin (c), Ku Mohd Kalkausar Ku Yusoff (a), Yuzwan Mohamad (a), Alyza Azzura Azmi (a), Maisarah Jaafar (a), Noorlin Mohamad (a), Wan Mohd Afiq Wan Mohd Khalik (a) a) Microplastic Research Interest Group (MRIG), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia b) Water Quality Laboratory, National Water Research Institute of Malaysia (NAHRIM), Lot 5377, Jalan Putra Permai, Rizab Melayu Sungai Kuyoh, 43300 Seri Kembangan, Selangor, Malaysia c) ALS Technichem (M) Sdn Bhd., Wisma ALS, No. 21, Jalan Astaka U8/84, Bukit Jelutong, 40150 Shah Alam, Selangor, Malaysia Abstract Microplastics ranging in size from 1 micrometre to 5 millimetre are high concern pollutants that are ubiquitous in the environment. Current studies have shown that most MPs in the seas are mainly from land and river inputs. This study presents the distribution and characteristics of MPs in the surface water of two major river basins of Malaysia, namely Langat and Kelantan rivers. MPs were classified based on sizes, shapes, colours and surface morphology. The average of 179.6 items/L and 1464.8 items/L of MPs had been quantified from both rivers, respectively. Fibre (91.90%) was highly recorded at Kelantan, compared to Langat whereby both fibre (59.21%) and fragment (38.87%) were prevalence. Micro FTIR analysis identified 14 polymers in Kelantan river, whereas 20 polymers were found in Langat river. Polypropylene, polyethylene, polyethylene terephthalate, nylon, phenoxy resins, poly(methyl acrylate), poly(methyl methacrylate), polystyrene, polytetrafluoroethylene, polyurethane and rayon were discovered in both rivers, although only polyethylene was significant (>1ppm) when further analysed using pyrolysis-GC/MS. Correlation analysis and multiple linear regression suggesting only turbidity was positively significant to the microplastic occurrence. This investigation is important for comprehending the abundance of MPs in the riverine system and the significance of in situ environmental parameters. Keywords: Riverine system, Estuary, Surface water, Plastic pollution,Polymers, South China Sea, Straits of Malacca Share Link | Plain Format | Corresponding Author (Yusof Shuaib Ibrahim)

65	Aquatic Pollution and Ecotoxicology	ABS-84
Heart Rate of Oryzias javanicus Embryos as Simple Biomarker to Detect the Effects of Triclosan Andi Dina Hardiana (a), Sri Wahyuni Rahim (a*), Khusnul Yaqin (a), Meimulya (a) a) Faculty of Marine Science and Fisheries, Hasanuddin University. Jalan Perintis Kemerdekaan KM 10, Makassar 90245, South Sulawesi, Indonesia. *yunirahim[at]yahoo.co.id Abstract Triclosan (TCS) is an antimicrobial agent that is widely found in antibacterial soap or disinfectant which has increased since Covid-19 and become a pollutant in aquatic environment. This study was intended to evaluate the effect of TCS with simple biomarker on Oryzias javanicus embryo. The embryos were exposed to TCS 0.2 mg/l from the neurula phase until hatching. The parameter measured was heart rate. The results of measuring the heart rate of embryos exposed to TCS and control group showed a statistically difference (p<0.05). After exposure, it was found that the TCS decreased the heart rate of O. javanicus embryos. This study concluded that TCS induced bradycardia of O. javanicus embryos and heart rate can be used as a simple biomarker to detect the effect of TCS. Keywords: Triclosan- Simple biomarker- Embryo- Oryzias javanicus- Heart rate Share Link | Plain Format | Corresponding Author (Andi Dina Hardiana)

66	Aquatic Pollution and Ecotoxicology	ABS-86
Semi-in situ exposure of Oryzias javanicus embryos (Bleeker, 1854) using heart rate as a biomarker Meimulya (a), Sri Wahyuni Rahim (a*), Khusnul Yaqin (a), Andi Dina Hardiana (a) (a) Faculty of Marine Science and Fisheries, Hasanuddin University. Perintis Kemerdekaan Street, KM 10 Makassar 90245, South Sulawesi, Indonesia. *yunirahim[at]yahoo.co.id Abstract Pollutants in waters originating from industrial and domestic wastes have the potential to harm the life of aquatic biota, especially during the early development phase or the embryonic phase. The presence and interaction between contaminants in water and aquatic biota need to be studied through ecotoxicological tests. The use of embryos in ecotoxicological tests is considered more effective and efficient because they have high sensitivity. This study aims to analyze the use of a simple biomarker, the heart rate of O. javanicus embryos for effects-based biomonitoring purposes. O. javanicus embryos were exposed to Tallo River water. Exposure begins at stadia 17 until the eggs hatch. The parameter measured was heart rate starting from stadia 24 to 37. The results showed that exposure to polluted water from the Tello River significantly caused a decrease in heart rate (bradycardia). This study showed that O. javanicus embryos had high sensitivity so that they could be used as sentinel organisms to detect contaminants semi-in situ. Keywords: Pollutants- sentinel organisms- Oryzias javanicus- heart rate- bradycardia Share Link | Plain Format | Corresponding Author (Meimulya Meimulya)

67	Aquatic Pollution and Ecotoxicology	ABS-92
Identification of Microplastics in Beach Sediments of Turtles Nesting Ground in Terengganu Aina Arifah Khalid (a,b), Maisarah Jaafar (a,b*), Tuan Nurul Sabiqah Tuan Anuar (a,b), Yusuf Shuaib Ibrahim (a.b) a) Microplastic Research Interest Group (MRIG), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, MALAYSIA b) Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia * maisarah[at]umt.edu.my Abstract Microplastics on beaches have the potential to alter the composition of shoreline sand, which could affect turtle incubation habitats. Thus, the aim of this study was to determine the abundance of microplastics in three different nesting grounds for green and hawksbill turtles in Terengganu, Malaysia, namely Chagar Hutang, Ma^ Daerah, and Setiu. The sediment samples were collected from a depth of 0-70 cm (with a depth interval of 10 cm) and chemically extracted using a modified WESTPEC method before being manually sorted using a stereo microscope. The abundance of microplastic in turtle nests was consistently found higher at 60-70 cm depth for all locations, with a range of abundance following the trend- Ma^ Daerah (792.5-1110.63 items/kg) \geq Setiu (602.50-763.13 items/kg) \geq Chagar Hutang (535.65-569.38 items/kg). The main shape of microplastics is fibre and fragment, with black being the dominant color (41.41%) and orange being the least (0.17%). Polymers such as Polyacrylamide, Polytetrafluorethylene, Ethylene vinyl alcohol, Phenol formaldehyde , Poly(vinyl alcohol), and Bisphenol A were identified under \muFTIR, which could be linked to human anthropogenic activities such as fishing and local human activities. Scanning electron microscope images of the microplastic surface show pit holes, cracks, and small dirt particles, indicating that the plastic particles have weathered and possibility for pollutants attachment. The findings from this study could provide a baseline data for future research associated with microplastic pollution in sea turtle nesting habitats, allowing for better protection of these endangered species in Malaysia. Keywords: microplastic, nesting turtle, polymers, sediment, South China Sea Share Link | Plain Format | Corresponding Author (Aina Arifah Khalid)

68	Aquatic Pollution and Ecotoxicology	ABS-119
BIOACCUMULATION OF HEAVY METALS IN BUNGO FISH (Glossogobius giuris Hamilton, 1822) IN LAKE TEMPE, SOUTH SULAWESI, INDONESIA 1Wayan Kantun 2Fatma 4Wilma Moka 1Nuraeni L Rapi and 3Awaluddin 1. Aquatic Resources, Institute of Technology and Maritime Business Balik Diwa 2. Marine Science, Institute of Technology and Maritime Business Balik Diwa 3. Fisheries Product Processing, Institute of Technology and Maritime Business Balik Diwa 4. Aquatic resources Management, Universitas Hasanuddin Abstract Heavy metals are a well-known indicator of water pollutants that can present adverse impact on living things. Of this reason, the study aims to probe the bioaccumulation of mercury and cadmium in Bungo fish that live in Lake Tempe. The study tapped into purposive sampling, i.e., collecting the fish from Lake Tempe situated in Wajo, Sidrap and Soppeng. It was a three-month research, starting from June to August 2021. Samples were tested in detection work by Atomic Absorption Spectrophotometry (AAS). Results of the bioaccumulation of heavy metals in Bungo fish varied according to the sampling locations. The average bioaccumulation of mercury in Wajo, Soppeng and Sidrap was 0,066 plus minus 0,005 mg/kg- 0,048 plus minus 0,006 mg/kg- and 0,059 plus minus 0,002 mg/kg, respectively. The average bioaccumulation of cadmium in Wajo, Soppeng and Sidrap was 0,016 plus minus 0,001 mg/kg- 0,030 plus minus 0,009 mg/kg- and 0,025 plus minus 0,005 mg/kg, respectively. Accordingly, Wajo had the highest degree of mercury bioaccumulation, while Soppeng had the highest degree of cadmium bioaccumulation. In other words, Wajo was the most mercury-polluted area, and Soppeng was the most cadmium-polluted area for Bungo fish. However, the substances in both areas remained below the maximum limits for water pollution. Keywords: Bungo Fish, Lake Tempe Share Link | Plain Format | Corresponding Author (Wilma Moka)

69	Aquatic Pollution and Ecotoxicology	ABS-121
Optimization of Chitosan coagulant from dry legs of Giant Freshwater Prawn, Macrobrachium rosenbergii in aquaculture wastewater treatment using Response Surface Methodology (RSM) Benedict Terkula Iber, Donald Torsabo, Che Engku Noramalina Che Engku Chik, Fachrul Wahab, Siti Rozaimah Sheikh Abdullah, Hassimi Abu Hassan, Nor Azman Kasan* Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Abstract The environmental impacts of aquaculture wastewater and biowaste from shellfish processing have become a major challenge to aquaculture expansion and sustainability. There is need to develop measures for the wastewater treatment and proper recycling of the biowaste. In this study, dry legs of giant freshwater prawn (Macrobrachium rosenbergii) were chemically processed into chitin and chitosan for use as coagulant in the coagulation/flocculation of real aquaculture wastewater. Box- Behnken design in response surface methodology (RSM) was used to establish the optimum operating conditions of chitosan dosage, pH, and settling time for removal of turbidity and colour. Statistical models were generated for the independent variables after which the model was validated. Results showed that dry legs of M. rosenbergii are mainly composed of minerals (76.36%) with low level of chitin (9.29%) and chitosan (7.02%). With 80% solubility and 91% degree of deacetylation (DDA), the smooth and none porous chitosan showed great potential of a good coagulant. This study recorded that dosage (5mg/L), pH (5) and settling time of 11.25 minutes successfully removed 93.84% turbidity while 99.89% colour removal was achieved at 20mg/L at 7.5 pH and 30 minutes settling time. The high level of minerals in the legs was responsible for the low chitin and chitosan yields while high turbidity and colour removals were attributed to the solubility and DDA. Keywords: Chitin, Chitosan, Coagulation/flocculation, Colour, RSM, Turbidity Share Link | Plain Format | Corresponding Author (Benedict Terkula Iber)

70	Aquatic Pollution and Ecotoxicology	ABS-128
Antibiotic Resistance in Vibrio parahaemolyticus Bacteria Isolated from Shrimp: Observation of Shrimp Ponds in South Sulawesi Province Hamzah, Srinawati, Alfa Astiana Afandi, Yuani Mundayana, Habson Batubara, Lideman, Sahabuddin, Akmal, Mochammad Syaichudin, Siti Faridah, Imam Sudrajat, Abdul Gafur. 1 Research Centre of Fishery, Research Organization on Earth Sciences and Maritime, National Research and Innovation Agency, Cibinong, West Java Province, Indonesia. 16911 2 Brackishwater Aquaculture Development Centre, Takalar, South Sulawesi Province, Indonesia. 92254 Abstract Abstract The presence of Vibrio parahemolyticus bacteria in shrimp farming ponds should be a concern because it is included in opportunistic pathogens. Vibrio parahemolyticus can be beneficial by aiding shrimp digestion, but under certain conditions and with which the population increases can adversely affect the condition of shrimp. Even today there has been a warning of the emergence of Vibrio parahemolyticus bacteria that are resistant to antibiotics, because considering the use of antibiotics in aquaculture activities, both in the hatchery stage and in the enlargement stage. The emergence of antibiotic-resistant bacteria is not only the result of the unwise use of antibiotics but can also be due to mutations from fellow bacteria that enter the aquatic environment. Therefore, it is important to ascertain whether there are Vibrio parahemolyticus bacteria that are resistant to antibiotics from shrimp-rearing activities in ponds. Efforts to obtain early detection results will be very important for policymakers related to the use of antibiotics in pond shrimp farming. In addition, it will be information for shrimp farmers about the effectiveness of the use of antibiotics that they have been doing. Observation of antibiotic resistance for Vibrio parahemolyticus bacteria, obtained from ponds in the South Sulawesi Province. Based on observations conducted in four districts, shows that there are no Vibrio parahemolyticus bacteria that are resistant to the 3 types of antibiotics tested. So it can be said that shrimp originating from ponds in South Sulawesi Province are still free from Vibrio parahemolyticus bacteria that are resistant to antibiotics, even so, we all still have to be vigilant and continue to monitor this problem. Keywords: Keywords : Resistance, Antibiotic, Shrimp, Pond Share Link | Plain Format | Corresponding Author (Hamzah -)

71	Aquatic Pollution and Ecotoxicology	ABS-141
Accumulation of microplastics in river basin sediments of Kuala Rompin, Malaysia (a)Nur Izzati Razak, (a)Sabiqah Tuan Anuar, (a)Yusof Shuaib Ibrahim, (a*)Maisarah Jaafar (a) Microplastics Research Interest Group (MRIG), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Malaysia Abstract Kuala Rompin is well-known for hosting annual world-class fishing competitions and for its plentiful supply of freshwater prawns and clams. However, the accumulation of microplastics in freshwater sediment can pose a hazard to aquatic organisms and enter the food chain. Thus, this study was undertaken to investigate the accumulation of microplastics in Rompin river sediment samples collected from 6 stations along a 20-kilometer stretch from the river to the open sea. 24 sediments were collected with a ponar grab and extracted with zinc chloride (1.63 g/cm3) and hydrogen peroxide (30%) before being physically sorted under stereomicroscope for shape, colour, and size identification. Microplastic abundances ranged from 213 to 455 particles/g, with particle sizes ranging from 30 to 300 microns. The most common shape and colour were fibres (90%) and transparent (68%) microplastics, respectively. The surface morphology of selected microplastics was cracked, with pit holes and irregular surface texture, indicating that the microplastic had weathered and may have interacted with other pollutants in the environment. Micro-Fourier Transform Infrared was used to confirm the polymer types of microplastics in selected samples, which can be linked to the local human anthropogenic sources. A high accumulation of microplastics in sediments could potentially affecting aquatic organisms, particularly filter feeders like clams, raising concerns about food safety when consumed. Keywords: Microplastic, Sediment, Kuala Rompin, food, South China Sea Share Link | Plain Format | Corresponding Author (NUR IZZATI BINTI ABD RAZAK)

72	Aquatic Pollution and Ecotoxicology	ABS-156
Microplastics in the Digestive Tract of Demersal and Pelagic Fish in Majene Regency, West Sulawesi Muh. Afdal (a*), Shinta Werorilangi (b,e), Ega Adi Wicaksono (c), Robby Nimzet (d) a) Department of Marine Science, Universitas Lambung Mangkurat, Jl. Jenderal A. Yani Km. 36 Banjarbaru Kalimantan Selatan 70714 , Indonesia *muh.afdal[at]ulm.ac.id b) Department of Marine Science, Universitas Hasanuddin, Jl Perintis Kemerdekaan km 10, Makassar 90245, Indonesia d) Department of Fisheries, Universitas Gadjah Mada, Yogyakarta, Indonesia d) Environmental Science Study Program, Universitas Hasanuddin, Jl Perintis Kemerdekaan km 10, Makassar 90245, Indonesia e) Marine Plastic Research Group, Universitas Hasanuddin, Jl Perintis Kemerdekaan km 10, Makassar 90245, Indonesia Abstract Microplastics are small particles <5 mm in size that originated from plastic waste. Microplastic contamination has spread in Indonesian waters, one of which is in the waters of Majene City, West Sulawesi. However, there has been no research related to the content of microplastics in demersal and pelagic fish in Majene Regency, West Sulawesi. This research aims to analyze microplastic contamination in demersal and pelagic fish and the abundance of microplastics in the digestive tract of demersal and pelagic fish. The research was conducted in Majene District, West Sulawesi. Fish samples were dissected and taken from the digestive tract, then KOH 10% was added 3 times the sample volume for digestive treatment. Analysis and identification were carried out at the Laboratory of Marine Ecotoxicology, Department of Marine Science, Faculty of Marine Science and Fisheries, Universitas Hasanuddin. The results showed that the highest contamination in demersal fish was the species Lethrinus semicinctus (40%), while pelagic fish was the species Decapterus macarellus (80%). The highest abundance of microplastics in the digestive tract of demersal fish was found in the species Lethrinus semicinctus of 1.53 (item/individual), while in pelagic fish it was found in the species Decapterus macarellus of 1.70 (item/individual). Based on the results of statistical tests, the abundance of microplastics in demersal fish was not significantly different, as well as in pelagic fish. Line is the highest form of microplastic found in demersal fish with a percentage of 66-100%, while line forms are found in pelagic fish with a percentage of 60-100%. These results indicated that the waters of Majene Regency have been contaminated with microplastics and have accumulated in demersal and pelagic fish which are mostly consumed by the people of Majene Regency, West Sulawesi. Keywords: Demersal fish- Pelagic fish- Microplastics- Majene Share Link | Plain Format | Corresponding Author (Muh. Afdal)

73	Aquatic Pollution and Ecotoxicology	ABS-157
Microplastic in the consumption fish from Tallo River, Makassar, Indonesia EA Wicaksono1, S Werorilangi2, M Afdal3, A Yusuf4, A Tahir2 1Department of Fisheries, Universitas Gadjah Mada, Yogyakarta, Indonesia 2Department of Marine Science, Universitas Hasanuddin, Makassar, Indonesia 3 Department of Marine Science, Universitas Lambung Mangkurat, Banjarmasin, Indonesia 4Fish Product Technology Study Program, Institut Teknologi Sains dan Bisnis Muhammadiyah Selayar, Selayar, Indonesia Abstract Microplastics have become one of the most studied pollutions in the aquatic environment. The Tallo River is one of the rivers known to be polluted by microplastics. This study aimed to determine the presence of microplastic pollution in fish captured from the Tallo River. Fish Sampling was carried out using a gill and lift net. Microplastic extraction was done by the alkaline digestion method. Six different species of fish were obtained from the Tallo River, and all of these species were contaminated with microplastics with average concentrations ranging from 0.6 - 2.4 items/individual. Based on the non-parametric Kruskal Wallis test, there was no difference in microplastic abundance between species (P=0.054). Most of the microplastics found are in the form line and blue colored. The presence of microplastics in the consumption fish in the Tallo River is an important concern for food safety. Keywords: Pollution, Plastics, Contamination, Fish Share Link | Plain Format | Corresponding Author (Ega Adhi Wicaksono)

74	Aquatic Pollution and Ecotoxicology	ABS-193
Water Quality Assessment and Pollution Status Analysis in Toro Rivers, Bone Regency Anton, Ihwan, Muhammad Syahrir, Yunarty, Diana Putri Renitasari, Ardana Kurniaji Aquaculture Technique Program Study, Polytechnic of Marine and Fisheries Bone, Bone, Indonesia Abstract This study aimed to assess the water quality in Toro River and to analyze the status of pollution using the Storet method according to the regulation on quality standards in Minister of Environment Decree number 115 of 2003 and the quality standards of Minister of Environment Decree No. 179 of 2004. The research was carried out for 3 months from September to November 2022, in the Toro River, Toro Village, Bone Regency. Data was taken directly in the field consisting of water samples at 10 stations and soil sediments at 5 stations randomly. The results showed that the range of water quality parameter values observed were Ammonia 0.12-0.31 mg/L, Phosphate 0.0021-0.0082 mg/L, Nitrate 0.16-2.41 mg/L, Nitrite 0.0055-0.018 mg/L, Cd 0.001-0.013 mg/L, Pb 0 mg/L, Temperature 32.1-34.4oC, DO 2.48-8.14 mg/L, pH 6.33-8.14, salinity 3-25 o/oo, BOD 2-5.12, COD 6.96-81.69 mg/L, BOT 14.26-46.79 mg/L. Based on a comparison of data per parameter with water quality standards from Minister of Environment Decree number 179 of 2004, it shows that the Nitrate and Cadmium (Cd) parameters have exceeded the water quality standard thresholds. Based on the analysis of the Storet value system it was known that the waters of the Toro River are included in the class C category, namely moderately polluted with a score of -28. Pollution control in the Toro River needs to be done in order to maintain environmental conditions that are not polluted. Keywords: Environment, Moderate Polluted, Storet Method, Water Quality Share Link | Plain Format | Corresponding Author (Ardana Kurniaji)

75	Aquatic Pollution and Ecotoxicology	ABS-199
Fractionation of Pb Metal in Marine Sediments as a Tool for Estimating Potential Bioavailability Values in Archipelagic Waters of North Maluku Province Inayah Unkhair University Abstract Determination of the geochemical fractionation of metals is also important to identify sources of metal pollutants in waters, whether they originate from natural processes or originate from human activities. The composition of the fraction in a resistant form indicates a source of metal pollutants originating from natural processes, while the non-resistant fraction components indicate a source of heavy metal pollutants originating from human activities (Teixeira et al. 2003 and Rodrigues & Formoso, 2006). The most important physical and chemical characteristics of sediments are the size of the sediment particles and the organic matter content (Rainbow, 2006). The research was carried out in the North Maluku Waters Region in March 2023 Analysis of concentrations of Pb, Cr and Hg metals in the bioavailable fraction of sediments was carried out at the Chemical Oceanography Laboratory and Fisheries Laboratory, Faculty of Marine and Fisheries Sciences. The results showed that the total value of Pb metal was 6.8518-16.5123, the F1 value = <0.0002 - 11.567. F2 value = <0.0002 - 7.6335, and F3 value = <0.0002-5.1766. Based on the quality standards set by CCME and ANZECC/ARMCANZ Guidelines it is still below the quality standards. Keywords: Fractination, Biovaibility Share Link | Plain Format | Corresponding Author (Inayah -)

76	Aquatic Pollution and Ecotoxicology	ABS-240
A PRELIMINARY STUDY: DISTRIBUTION AND ABUNDANCE OF MICROPLASTIC IN RIVERS OF SOUTH SULAWESI Shinta Werorilangi*1,5, Tri Harianto2, Ahmad Faizal1,5, Wilma Moka3, and Muh. Afdal4 1Department of Marine Science, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Makassar - Indonesia 2Department Civil Engineering, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Makassar - Indonesia 3Aquatic Resource Management Study Program, Department of Fisheriesm Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Makassar - Indonesia 4Department of Marine Science, Lambung Mangkurat University, Banjarmasin - Indonesia 5Marine Plastic Research Group, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Makassar - Indonesia *Corresponding author : shintawk[at]unhas.ac.id ( +628152504202) Abstract The population increase in urban areas and densely populated areas in South Sulawesi has had an effect on decreasing water quality. Anthropogenic activity in the watershed makes the river a route for microplastic (MP) contaminants to enter the open waters. This preliminary research aims to broadly analyze the distribution and abundance of microplastics in watersheds in densely populated areas in South Sulawesi. The sample compartment consists of taking samples of fish and gastropod biota by digestion method with KoH. Water was collected using a Neuston net and sediment samples were collected using a sediment corer. The results showed that samples in three areas, namely Cenrana River (Bone Regency), Maros River (Maros Regency), Kariango River (Pinrang Regency) had varying abundance of MP in fish and gastropod ranging from 0.3-3.2 items/ind. The surface water samples showed MP varying from 0.42 - 0.67 items/liter. Whereas in sediments the MP abundance range is 33.3-100 items/kg. The MP characteristics found were fragments (23%), films (0.6%), foams (0.2%), and lines (76%) with dominant color variations of blue, transparent, and white. MP size is in the range of 0.02-3.9 mm. Keywords: Microplastics, Rivers, South of Sulawesi, Organisms, Sediments, Surface Water Share Link | Plain Format | Corresponding Author (Shinta Werorilangi)

77	Marine and Fisheries Biotechnology	ABS-2
Semi-Polar Fraction of Soft Coral Nepthea Sp.: Chemical Profile, Antioxidant and Cytotoxic Potencies Sahidin1, Baru Sadarun2, Nur Syifa Rahmatika3, Agung Wibawa Mahatva Yodha4, Adryan Fristiohady1 1Faculty of Pharmacy, Universitas Halu Oleo, Kendari, INDONESIA 2Faculty of Marine Sciences and Fisheries, Universitas Halu oleo, Kendari, INDONESIA 3Faculty of Medicine, Universitas Halu Oleo, Kendari, INDONESIA 4Health Polytechnics of Bina Husada, Kendari, INDONESIA Abstract The propose of this research is to study on chemical and pharmaceutical aspects of soft coral Nepthea sp. The sample was extracted by ethylacetate (EAE) and then fractionated using vacuum liquid chromatrography (VLC). The chemical content was analyzed by phytochemical screening, LC-MS/MS analysis, Total Phenolics Content (TPC) and Total Flavonoids Contents (TFC). Antioxidant potency was evaluated by DPPH and ABTS methods. Cytotoxicity was analyzed by MTT assays. The result showed that the fractionation produced six fractions (1-6). Fraction 3 and 4 were semi-polar compounds, which contained terpenoids, phenolics and alkaloids. Based on LC-MS/MS data, the identified semi-polar compounds consist of 3-acetyl-3,4-dihydro-5,6-dimethoxy-2(1)H-benzopyrone and oxyphyllenone B from Fraction 3 and 1-(4-Hydroxy-3-methoxy)-phenyl-1,2,3-propanetriol, digiprolactone, petasitolone, &#948--Humulene, rengyolester, and piperolactam-C9:1(8E) for Fraction 4. The antioxidant potency of fraction 4 was better than Fraction 3, which is supported by TPC Fraction 4>Fraction 3 with active antioxidant category for Fraction 4 and Fraction 3 is moderatery active antioxidant category. Based on IC50 value, Fraction 3 is active category and Fraction 4 is moderately active category against Breast Cancer cell lines MCF-7. The semi-polar fraction of Nepthea sp. can be developed as raw material for antioxidant and anticancer. Keywords: Nepthea sp., Semi-polar fraction, Chemical, Antioxidant, Cytotoxic Share Link | Plain Format | Corresponding Author (I. Sahidin)

78	Marine and Fisheries Biotechnology	ABS-5
Analysis of antivibrio compounds from mangrove sediment and marine sponge-associated bacteria Ifhan Dwinhoven (1), Triyanto(2), Noer Kasanah(2) 1Fish Hatchery Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, Poros Makassar-Pare Pare Km 83, South Sulawesi, Indonesia 2Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia Abstract Vibriosis is a major concern in aquaculture causing rapid large amounts of fish death and economic loose. Thus, the new antibacterial compounds which are more specific and selective for Vibrio sp. are needed through the exploration of marine resources. The aim of this study to determine the chemical profiles of antivibrio compounds by mangrove sediment and marine sponge-associated bacteria. The mangrove sediment originated from the coastal of Situbondo, Cilacap, Rembang, and Palembang, while the sponges were collected from Manado. The secondary metabolites fermentation was carried out in various media, then the supernatants was tested for microtiter bioassay against V. alginolyticus, V. harveyi and V. parahaemolyticus in 96-well plate. Detection of bioactive compounds were done by Thin Layer Chromatography (TLC) with indicators of UV 254 nm and UV 366 nm, and TLC staining using ninhydrin, p-anisaldehyde, and H2SO4 reagents. The screening results showed that all the samples possessed bioactivity against Vibrio sp.. TLC results showed that the CIL-10-2016-01/02 and TPMD-13-01/02 were predicted to contain peptide compounds with pink discoloration on the TLC spot after dyeing with ninhydrin. The results of bioautography assay showed that isolates were active against V. harveyi and V. parahaemolyticus at Rf 0.16 and at Rf 0.57, respectively which indicated that the compound is a peptide molecule. H-NMR spectra indicated that there were peptide compounds in the combination bacterial isolates CIL-10-2016-01/02 and TPMD-13-01/02. Thus , the results showed that the combination bacterial isolates was capable to produce antivibrio compounds Keywords: Antibacterial- Bioactive compounds- Peptide- Vibriosis Share Link | Plain Format | Corresponding Author (Ifhan Dwinhoven)

79	Marine and Fisheries Biotechnology	ABS-14
Identification of Vibrio spp Bacteria from The Environment of Research Station of Tiger Shrimp Hatchery in Barru, South Sulawesi as Candidates for Host Bacteriophages Muliani, Nurbaya, Endang Susianingsih, Nurhidayah, dan Gunarto Fisheries Research Center, National Research and Innovation Agency, Cibinong, Bogor, West Java, Indonesia. Abstract This study aims to isolate and identify Vibrio spp bacteria as candidates for bacteriophage hosts from the environment of the Research Station of Tiger Shrimp Hatchery in Barru, South Sulawesi. Research is carried out by isolation, morphological identification, and molecular identification of Vibrio sp bacteria as host bacteriophages, as well as isolation of bacteriophages and determination of host range of bacteriophages. The number of Vibrio spp bacteria isolated from the hatchery environment were obtained in 39 isolates, namely, sixteen from shrimp larvae, ten from water media for shrimp larvae, five from water media and hemolimp of mud crab broodstock, two from shrimp hemolymph, two from water in around of cage culture, two from shrimp ponds, and one isolate each from sourcewaters and wastewaters. Seven of the 39 isolates were molecularly identified and four isolates were identified as Vibrio parahaemolyticus with proximity indexes 99.09-99.72%. The three isolates remaining were identified as V. alginolyticus (99.30%), V. sinaloensis (98.26%), and Photobacterium sp (95.07%). Bacteriophage isolation using V. parahaemolyticus as the host was obtained 11 samples containing bacteriophages from 28 samples. It was indicated that water and sediment in the environment of the Research Station for tiger shrimp hatchery were potential as sources of bacteriophages. The host range test showed that V.parahaemolyticus phages were obtained from the Research Station of tiger shrimp hatchery in Barru could infect at least 8 Vibrio spp isolates from the 20 bacterial isolates tested. This indicates that the bacteriophage of V. parahaemolyticus has a fairly high host range Keywords: Vibrio spp, Vibrio parahemolyticus, Bakteriofage, Host Share Link | Plain Format | Corresponding Author (Muliani Muliani)

80	Marine and Fisheries Biotechnology	ABS-29
The potential of banana weevil extract (Musa acuminata) in prospective tiger shrimp (Penaeus monodon. fab) broodstock E Suryati 1, S Lante1, A Tenriulo 1, R Rosmiati 1, A Parenrengi 1, H Herlinah 1 Center of Fisheries Research, National Research, and Innovation Agency Abstract Musa Acuminata contains serotonin, a neurotransmitter compound that plays a role in the reproductive process in crustaceans, used to spur the development of reproductive organs in prospective mother tiger shrimp. In this research activity, serotonin isolation from banana weevils was carried out through extraction and maceration techniques as well as purification using acetone and alcohol. The application of banana weevil extract was carried out through injection with concentrations of 0, 25, 50, and 75 ug /g BW, as well as ablation of the eye stalk as a positive control, the study design used a complete randomized design (CRD) with 5 treatments and 3 repeats. Observations include survival, frequency of molting, gonadal maturity level with PCR technique using vitellogenin and LvDMC primers, histology, and total hemocyte. The isolates obtained are serotonin compounds based on purity tests with HPLC, as well as UV and IR spectrum measurements. The results of extract testing on shrimp mothers showed the highest survival in the treatment of 50 and 75 ug /g BW, as well as molting and gonadal development both through PCR, as well as histology, showed a positive response, It is hoped that quality tiger shrimp broodstock candidates can be obtained Keywords: Musa Acuminata ,serotonin, shrimp broodstock Share Link | Plain Format | Corresponding Author (Emma Suryati)

81	Marine and Fisheries Biotechnology	ABS-31
SUPLEMENTASI TEPUNG KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) SEBAGAI PREBIOTIK PADA IKAN NILA (Oreochromis niloticus) Agustina*, Henny Pagoray, Riska Fadhilah Program Studi Akuakultur Departemen Budidaya Perairan Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman * Corresponding email: agustina[at]fpik.unmul.ac.id Abstract Kulit buah naga merah (Hylocereus polyrhizus) merupakan limbah organik yang belum banyak dimanfaatkan dengan kandungan nutrisi berupa serat, karoten, kalsium fosfor dan vitamin B dan Vitamin C sebagai antibakteri dan antioksidan. Penelitian ini dilakukan untuk menganalisis penambahan tepung kulit buah naga merah (H. polyrhizus) sebagai prebiotik bagi ikan nila (O. niloticus). Benih ikan nila dengan ukuran rata-rata 9.5 cm dipelihara sebanyak sepuluh ekor selama 30 hari dalam akuarium bervolume 30 L. Ikan diberi makan sebanyak tiga kali sehari secara at satiation dengan pakan perlakuan yaitu P0 0% TKBN, P1 5% TKBN, P2 10% TKBN, dan 15% TKBN per kg pakan. Parameter yang diamati meliputi jumlah bakteri asam laktat dalam usus ikan, parameter hematologis, tingkat kelangsungan hidup, kinerja pertumbuhan dan pemanfaatan pakan. Hasil penelitian menunjukkan bahwa meningkatnya persentase tepung kulit buah naga berpengaruh nyata dalam meningkatnya jumlah bakteri asam laktat dalam usus ikan, kinerja pertumbuhan serta pemanfaatan pakan. Parameter hematologi menunjukkan kisaran yang normal serta tidak mempengaruhi tingkat kelangsungan hidup ikan nila. Berdasarkan hal ini maka tepung kulit buah naga merah berpotensi sebagai prebiotik pada benih ikan nila merah sampai dosis 15%. Keywords: Tepung kulit buah naga, prebiotik, ikan nila, kinerja pertumbuhan, parameter hematologi Share Link | Plain Format | Corresponding Author (Agustina Agustina)

82	Marine and Fisheries Biotechnology	ABS-43
THE INVESTIGATION OF the Ipomea Pes-Caprae LEAVES EXTRACT AS ANTIMICROBIAL OF Staphylococcus aureus Mohamad Gazali, Chair Rani, Widyastuti Umar, M. Ali S, Shafira Ananda Widya Fadly, Rina Syafitri Department of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University Department of Marine Science, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Deparment of Biology Education, Faculty of Education, Syiah Kuala University Student of Marine Science, Faculty of Fisheries and Marine Science Teuku Umar University Department of Agribusiness, Faculty of Agriculture, Teuku Umar University Abstract One of the health issues confronting Indonesia, particularly Aceh Province, is infectious disease. The bacteria Staphylococcus aureus is one of the causes of the infection. A frequent species on the beach of Labuhan Haji in South Aceh, Ipomoea pes-caprae (L.) Sweet (Convolvulaceae), is regarded as a potential source of bioactive chemicals. The purpose of this study is to compare I. pes-caprae to S. aureus microorganisms. Disk diffusion agar methods can be used to test antibacterial activity. The positive control in this study was ampicillin, whereas the negative control was aquadest. The experimental group and the control group were tested on S. aureus, and the treatments for the experimental group were repeated three times. The biggest inhibitory zone of I. pes-caprae was discovered at 100% concentration level (15.8 mm), followed by 80% concentration (12.5 mm), 60% (8.63 mm), 40% (2.3 mm), 20% (0 mm), and 10% concentration (2.2 mm). Also, in 80% concentration (11.4 mm), aquades extract had considerable inhibitory effect, whereas methanol extract had no inhibitory activity at any concentration level. It appears that ethyl acetate extract has the greatest ability to combat S. aureus bacteria. As a result, it is possible to conclude that I. pes-caprae leaf extract exhibits antibacterial properties against the growth of S. aureus. Keywords: Antimicrobial, Ipomea pes-caprae, Staphylococcus aureus Share Link | Plain Format | Corresponding Author (MOHAMAD GAZALI)

83	Marine and Fisheries Biotechnology	ABS-44
The Study of Potential Bioactive Compound of Enhalus acoroides as Antioxidant Agent from Pabisi Island Aceh Singkil Mohamad Gazali, Widyastuti Umar, Chair Rani, Rina Syafitri Department of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University, West Aceh, Indonesia Department of Marine Science, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Department of Agribusiness, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia Abstract Enhalus acoroides is the monotypic marine genus Enhalus in the family Hydrocharitaceae that distributed widely in the intertidal zone of Pabisi Island Aceh Singkil. E. acoroides is traditionally utilized by the local community for cake and biopharmca product. The objective of this study is to screen the potential bioactive compound of E. acoroides extracts as an antioxidant from Banyak Island, Aceh Singkil. The sample of E. acoroides was collected from Pulau Banyak, Aceh Singkil. The research method of these experiments includes phytochemical analysis and antioxidant assay. The research result showed the highest rendement of E. acoroides extract was yielded from n-hexane extract as much as 1.39%. The lowest rendement from ethanol is as much as 0.470%. According to phytochemical analysis showed triterpenoid and phenol compounds are contained in E. acoroides extract. The antioxidant activity in CUPRAC method showed that the ethanol extract was 65.40 mol trolox/g extract. Moreover, the ethyl acetate extract was 21.41 mol Trolox/g extract, and n-hexane extract yield 22.11 mol Trolox/g extract. In FRAP analysis also exhibit ethanol extract (679.50 mol Trolox/g extract), ethyl acetate (589.50 mol Trolox/g extract) and n-hexane extract (346.50 mol Trolox/g extract). Therefore, the discovery of bioactive compound has an important role in nature. Keywords: Aceh, Enhalus acoroides, marine, seagrasses Share Link | Plain Format | Corresponding Author (MOHAMAD GAZALI)

84	Marine and Fisheries Biotechnology	ABS-59
Fishing Ground and Composition of Catches of Large Pelagic Fish Using Hand line Tuna in FADs Area Arham Rumpa (a), Muh. Soghirun (a*), Ani Leilani (a), Nurdin Kasim (a), Rafi Ohorella (a), A. Riza Baroqi (b), Putra Satria Timur (b) a) Lecturer of Polytechnic of Marine and Fisheries Bone, 92718, Bone Regency, South Sulawesi, Indonesia * oghisupm[at]gmail.com b) Indonesian Fisheries and Society Foundation (MDPI), Denpasar, 80223, Bali, Indonesia Abstract FADs have been used by hand line tuna fishermen in Bone bay. Information on the characteristics of catching hand line tuna in the FAD area is very limited as material for studying tuna fishing strategies and policies. The research aims to understand the dynamics of fishing ground FADs and the composition of tuna catches. The data used is the result of observations using four ships based at PPI Lonrae, collecting data on 185 trips over 3 years (2020, 2021, and 2022). The results showed that the conditions at the installation location for FADs were very irregular and it was seen that hand line Tuna fishermen caught two WPPs, namely 713 and 714. There were concerns that fishermen would make arrests at 714 in the FAD area. The catch was dominated yellow fin tuna (Thunnus albacares) 66.25%, big eye tuna (Thunnus obesus) 19.14%, skipjack tuna (Katsuwonus pelamis) 1.13% and discard 7.48%. The size of yellow fin tuna caught was 69.67% between 41-51 cm in length and 45.36% for big eye tuna between 41-51 cm, while skipjack tuna was 46.48% between 31-40 cm and 52.88% in 41-50 cm. For an average catch rate of 806.4 kg/trip with a trip length of 10-15 days. These findings show that for 3 years, composition of hand line tuna catches in FADs still dominated by small tuna. It is necessary to regulate FAD management and hand line fishermen need to be directed to only catch fish that are worth catching in the FAD area. Keywords: Fishermen, hand line, large pelagic, management, FADs Share Link | Plain Format | Corresponding Author (Muh. Soghirun)

85	Marine and Fisheries Biotechnology	ABS-68
Chemical Characteristics of Cultivated Green Seaweed Caulerpa racemosa from Sinjai, South Sulawesi, Indonesia A Daud (a,b), Metusalach (c), Kasmiati (c*), and Nursinah Amir (c) a) Graduate School of Fishery Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia. b) Biochemistry Laboratory, Department of Fishery Product Processing Technology, The Pangkep State Agricultural Polytechnic, Pangkajene Kepulauan, South Sulawesi Indonesia c) Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, South Sulawesi, Indonesia. *Corresponding author: kasmiati[at]unhas.ac.id Abstract Several studies have described the nutraceutical properties of Caulerpa racemosa. However, the study related to the primary metabolite content of cultivated C. racemosa in Indonesia is scanty. Therefore, this study aimed to analyze the proximate compositions of the C. racemosa in two different forms - fresh and dried forms obtained from Sinjai, South Sulawesi. Results showed that fresh C. racemosa contained 89.58% moisture, 1.23% ash, 2.75% protein, 0.67% lipid, and 5.58% carbohydrate, while dried C. racemosa contained 11.38% moisture, 21.59% ash, 6.75% protein, 1.49% lipid, and 58.57% carbohydrate. This study suggests that postharvest handling of C. racemosa is vital to determine its quality as a nutritious and functional food source, as well as for value-added product development. Keywords: Caulerpa racemosa, proximate analysis, seaweed, sea grapes Share Link | Plain Format | Corresponding Author (AHMAD DAUD)

86	Marine and Fisheries Biotechnology	ABS-81
OPTIMASI PRODUKSI HIDROLISAT PROTEIN IKAN PATIN MENGGUNAKAN ENZIM ALCALASE Rahmi Nurdiani, Taufiq Rizki Abdurrahman, Muhamad Firdaus, Muhammad Rayhansyah Jati, Syaravina Ifilah M, Elfriede Rositta Debataraja Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya Malang Abstract Hasil samping pengolahan hasil perikanan merupakan sisa selama proses produksi yang dianggap tidak dapat diolah lebih lanjut walau sebenarnya memiliki nilai ekonomis yang tinggi jika diolah dengan baik. Hasil samping trimming ikan Patin (Pangasius sp.) memiliki kandungan protein yang tinggi sehingga sangat potensial diproduksi menjadi hidrolisat protein ikan (HPI). Penggunaan enzim merupakan metode yang efektif untuk produksi HPI. Enzim yang dipilih pada penelitian ini adalah alcalase karena dinilai mampu menghidrolisis protein bahkan yang telah terdenaturasi. Tujuan penelitian ini adalah untuk optimasi konsentrasi enzim alcalase dan lama inkubasi dalam proses produksi hidrolisat protein dari trimming ikan patin (Pangasius sp.). Penelitian ini menggunakan metode eksperimen dengan bantuan software Design Expert v13 dengan Response Surface Method model Central Composite Design untuk mengetahui kondisi produksi hidrolisat protein yang optimal dengan derajat hidrolisis, pH, dan antioksidan dengan uji nilai %inhibisi sebagai response. Hasil penelitian menunjukkan konsentrasi alcalase dan lama inkubasi yang berbeda berpengaruh nyata terhadap respon derajat hidrolisis, respon antioksidan yaitu nilai %inhibisi, dan respon nilai pH. Konsentrasi alcalase dan lama inkubasi yang paling optimum adalah sebesar 0,002 AU/g selama 143,649 menit dengan prediksi nilai derajat hidrolisis sebesar 48,056%, pH dengan nilai 7,41, dan nilai antioksidan sebesar 45,986% dengan nilai desirability untuk solusi pada formulasi hidrolisat protein dari trimming ikan patin adalah 0,926. HPI dari trimming ikan Patin berpotensi untuk diproduksi berkelanjutan karena memiliki bioaktivitas antioksidan yang tinggi. Keywords: Hidrolisat protein ikan, trimming ikan patin, optimasi Share Link | Plain Format | Corresponding Author (Rahmi Nurdiani)

87	Marine and Fisheries Biotechnology	ABS-87
Sargassum aquifolium juice induces IL-4 expression in DM 2 rats Muhamad Firdaus1*, Yoga Dwi Jatmiko2, Retno Tri Astuti1, Herlina Pratiwi3, and Ahmad Faris Priambodo1 1Faculty of Fisheries and Marine Sciences, Universitas Brawijaya, Malang, Indonesia 2Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia 3Faculty of Veterinary, Universitas Brawijaya, Malang, Indonesia Abstract Hyperglycemia decreases the expression of anti-inflammatory cytokines. Sargassum sp contains compounds that their bioactivity as anti-hyperglycemia. This study evaluated the Sargassum aquifolium extract to express anti-inflammation in Diabetes Mellitus (DM) 2 rats model. S. aquifolium was obtained from Ekas Bay, Lombok Island. A Male (Rattus norvegicus) was fed an atherogenic diet for 16 weeks and then injected peritoneally with streptozotocin (20 mg/dL). This study was grouped into healthy, diabetes + aquades, diabetes + 1,5 mL of S. aquifolium juice, diabetes + 3,0 mL of S. aquifolium juice, and diabetes + 4,5 mL of S. aquifolium juice. The parameter of this study was IL-4 expression. The relative percentage of IL-4 from Peripheral Blood Mononuclear Cells (PBMC) was measured using flow cytometry. The result showed that IL-4 expression in healthy groups was significantly higher than in diabetic groups. IL-4 expression in the diabetic group administered with 3 mL of S. aquifolium juice was significantly higher than in the diabetic group administered with aquadest or other volumes of S. aquifolium juice. Prevention of increased blood glucose by S. aquifolium juice reduces inflammation of diabetes. In conclusion that 1,5 mL of S. aquifolium juice increased IL-4 expression and prevented inflammation in diabetes. Keywords: S. aquifolium juice, diabetes mellitus type 2, inflammation, Interleukin-4 Share Link | Plain Format | Corresponding Author (Muhamad Firdaus)

88	Marine and Fisheries Biotechnology	ABS-90
The Effect of Different Carbohydrate Sources and Salt Concentration on Microbiology and Chemical Umami Characteristics of Bekasam from Tilapia (Oreochromis niloticus) Anis Miftachurrochmah, Wenny Bekti Sunarharum, Kiki Fibrianto Department of Food Science and Technology, Faculty of Agricultural Technology, Universitas Brawijaya Abstract Bekasam is a fermented fish product that is found in several regions of Indonesia, especially in South Sumatra, South Borneo, and North Sulawesi. Bekasam fermentation is a spontaneous fermentation that utilizes the activity of lactic acid bacteria using carbohydrates and salt for seven days of fermentation. Bekasam is generally made using freshwater fish, one of which is tilapia (Oreochromis niloticus). It has a sweet, sour, and delicious taste allowing it to be added as a flavoring to dishes or chili sauce. The synergistic combination of fermented compounds, such as amino and organic acids, can produce a savory or umami taste. Previously somebody knew that chemical and microbiological characteristics could affect the intensity of umami taste in fermented products such as crab sauce, rusip, etc. This study aims to analyze the microbiological and chemical characteristics of bekasam products from tilapia with different carbohydrate sources (A1: white rice, A2: white rice + brown sugar) and salt concentration (B1: 10%, B2: 12.5%, B3: 15%). The results showed that differences in carbohydrate sources and salt concentrations could produce significant differences (p<0.05) in the total number of lactic acid bacteria (6.61-7.47 log CFU/ml), pH value (5.36-6.13), total acid (0.021-0.035% ), water content (65.58-67.85%), protein (2.80-5.24%), and composition of total amino acids (especially glutamic acid and aspartate). Keywords: bekasam, tilapia (Oreochromis niloticus), umami characteristics Share Link | Plain Format | Corresponding Author (Anis Miftachurrochmah)

89	Marine and Fisheries Biotechnology	ABS-103
GROWTH AND QUALITY OF SEAWEED Eucheuma spinosum AND Kappaphycusalvarezii AT DIFFERENT LOCATIONS AND SEASONS Muhammad Syahrir Politeknik Kelautan dan Perikanan Bone, Jl. Sungai Musi KM 9 Pallete, Tanete Riattang Timur, Kabupaten Bone Abstract Seaweed is a macroalgae that has the potential to be developed in coastal areas. K. alvarezii and E.spinosum are international producers of keraginineextract. The limiting factors that affect the growth and quality of seaweed are season and location. The purpose of this study was to analyze the growth and quality of two species of seaweed at different locations and seasons. This research was conducted from January to December 2022. The weight of the seeds seaweed used is 50 grams, which is planted at a distance of 100 meters to 1 km and more than 1 km from the coast in different seasons. This research was conducted in Pallete Waters. The results of water quality studies such as temperature, pH, salinity did not differ by location or season, while current velocity, CO2 and NO3 were very different from planting locations and seasons. Distances of more than 1 km from the coast have lower absolute growth than distances of less than 1 km. During the rainy season the growth is best for both types of seaweed. Viscosity content planted at a location distance of more than 1 km is higher than 100 to 1 km from the coast. Carotenoids have higher levels at a distance of 100 m to 1 km compared to a distance of 1 km from the coast. Keywords: E.spinosum, K. alvarezii, Growth , Viscosity, Water Quality Share Link | Plain Format | Corresponding Author (Muhammad Syahrir)

90	Marine and Fisheries Biotechnology	ABS-108
PRELIMINARY GENETICS ASSESSMENT OF OYSTER CRASSOSTREA SPP FROM NORTHERN PENINSULAR MALAYSIA Syamim Balqis Shamsul Bahari (a), Rabi Atun Adawiah Abdullah (a), Mohamad Akib Baharom (b), Nik Muhammad Firdhaus (b), Norainy Mohd Husin (a*) a) Institute of Tropical Aquaculture and Fisheries (AKUATROP), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. *norainyhusin[at]umt.edu.my b) Lot 664 & 665, Tanjung Kuala, Kampung Pulau Gajah, 16100 Kota Bharu, Kelantan. Abstract Crassostrea oyster is one of the well-known oyster in Malaysia. There are various kind of oysters that can be found in Malaysia and among them are Crassostrea iredalei, Crassostrea belcheri, Saccostrea cucullata, Ostrea folium, and Hyotissa hyotis. The high demand of Crassostrea oyster for commercial purposes is the main reason for establishment of its aquaculture and this will reduce the threat to its natural populations. This study used the sample of Crassostrea oyster from Kelantan and Kedah, Malaysia. The objective of the study is to identify the exact Crassostrea species that can be found in Kelantan and Kedah by using mitochondrial cytochrome oxidase subunit I (COI) gene. The study also identifies and compare the population genetics of Crassostrea from the two locations. The BLAST results showed that the sample from Kelantan are Crassostrea iredalei and Crassostrea belcheri while from Kedah are Crassostrea iredalei and Crassostrea saidii. The results showed low to moderate nucleotide diversity (0.0006-0.007) and moderate to high haplotype diversity (0.4-0.9). The phylogenetic neighbour-joining tree analysis shows better understanding between species and populations. The results of this study have significant potentials for Malaysia aquaculture industry, population control and monitoring as well as wild oyster species protection in the future. Keywords: Crassostrea sp., Population genetics, CO1, Phylogenetic tree, Aquaculture Share Link | Plain Format | Corresponding Author (SYAMIM BALQIS SHAMSUL BAHARI)

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