Identification of Vibrio spp Bacteria from The Environment of Research Station of Tiger Shrimp Hatchery in Barru, South Sulawesi as Candidates for Host Bacteriophages
Muliani, Nurbaya, Endang Susianingsih, Nurhidayah, dan Gunarto

Fisheries Research Center, National Research and Innovation Agency, Cibinong, Bogor, West Java, Indonesia.

Abstract

This study aims to isolate and identify Vibrio spp bacteria as candidates for bacteriophage hosts from the environment of the Research Station of Tiger Shrimp Hatchery in Barru, South Sulawesi. Research is carried out by isolation, morphological identification, and molecular identification of Vibrio sp bacteria as host bacteriophages, as well as isolation of bacteriophages and determination of host range of bacteriophages. The number of Vibrio spp bacteria isolated from the hatchery environment were obtained in 39 isolates, namely, sixteen from shrimp larvae, ten from water media for shrimp larvae, five from water media and hemolimp of mud crab broodstock, two from shrimp hemolymph, two from water in around of cage culture, two from shrimp ponds, and one isolate each from sourcewaters and wastewaters. Seven of the 39 isolates were molecularly identified and four isolates were identified as Vibrio parahaemolyticus with proximity indexes 99.09-99.72%. The three isolates remaining were identified as V. alginolyticus (99.30%), V. sinaloensis (98.26%), and Photobacterium sp (95.07%). Bacteriophage isolation using V. parahaemolyticus as the host was obtained 11 samples containing bacteriophages from 28 samples. It was indicated that water and sediment in the environment of the Research Station for tiger shrimp hatchery were potential as sources of bacteriophages. The host range test showed that V.parahaemolyticus phages were obtained from the Research Station of tiger shrimp hatchery in Barru could infect at least 8 Vibrio spp isolates from the 20 bacterial isolates tested. This indicates that the bacteriophage of V. parahaemolyticus has a fairly high host range

Keywords: Vibrio spp, Vibrio parahemolyticus, Bakteriofage, Host

Topic: Marine and Fisheries Biotechnology