The Study of Potential Bioactive Compound of Enhalus acoroides as Antioxidant Agent from Pabisi Island Aceh Singkil Mohamad Gazali, Widyastuti Umar, Chair Rani, Rina Syafitri
Department of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University, West Aceh, Indonesia
Department of Marine Science, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia
Department of Agribusiness, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia
Abstract
Enhalus acoroides is the monotypic marine genus Enhalus in the family Hydrocharitaceae that distributed widely in the intertidal zone of Pabisi Island Aceh Singkil. E. acoroides is traditionally utilized by the local community for cake and biopharmca product. The objective of this study is to screen the potential bioactive compound of E. acoroides extracts as an antioxidant from Banyak Island, Aceh Singkil. The sample of E. acoroides was collected from Pulau Banyak, Aceh Singkil. The research method of these experiments includes phytochemical analysis and antioxidant assay. The research result showed the highest rendement of E. acoroides extract was yielded from n-hexane extract as much as 1.39%. The lowest rendement from ethanol is as much as 0.470%. According to phytochemical analysis showed triterpenoid and phenol compounds are contained in E. acoroides extract. The antioxidant activity in CUPRAC method showed that the ethanol extract was 65.40 mol trolox/g extract. Moreover, the ethyl acetate extract was 21.41 mol Trolox/g extract, and n-hexane extract yield 22.11 mol Trolox/g extract. In FRAP analysis also exhibit ethanol extract (679.50 mol Trolox/g extract), ethyl acetate (589.50 mol Trolox/g extract) and n-hexane extract (346.50 mol Trolox/g extract). Therefore, the discovery of bioactive compound has an important role in nature.
