The potential of banana weevil extract (Musa acuminata) in prospective tiger shrimp (Penaeus monodon. fab) broodstock E Suryati 1, S Lante1, A Tenriulo 1, R Rosmiati 1, A Parenrengi 1, H Herlinah 1
Center of Fisheries Research, National Research, and Innovation Agency
Abstract
Musa Acuminata contains serotonin, a neurotransmitter compound that plays a role in the reproductive process in crustaceans, used to spur the development of reproductive organs in prospective mother tiger shrimp. In this research activity, serotonin isolation from banana weevils was carried out through extraction and maceration techniques as well as purification using acetone and alcohol. The application of banana weevil extract was carried out through injection with concentrations of 0, 25, 50, and 75 ug /g BW, as well as ablation of the eye stalk as a positive control, the study design used a complete randomized design (CRD) with 5 treatments and 3 repeats. Observations include survival, frequency of molting, gonadal maturity level with PCR technique using vitellogenin and LvDMC primers, histology, and total hemocyte. The isolates obtained are serotonin compounds based on purity tests with HPLC, as well as UV and IR spectrum measurements. The results of extract testing on shrimp mothers showed the highest survival in the treatment of 50 and 75 ug /g BW, as well as molting and gonadal development both through PCR, as well as histology, showed a positive response, It is hoped that quality tiger shrimp broodstock candidates can be obtained
Keywords: Musa Acuminata ,serotonin, shrimp broodstock
