Analysis of antivibrio compounds from mangrove sediment and marine sponge-associated bacteria Ifhan Dwinhoven (1), Triyanto(2), Noer Kasanah(2)
1Fish Hatchery Technology Study Program, Department of Aquaculture, Pangkep State Polytechnic of Agriculture, Poros Makassar-Pare Pare Km 83, South Sulawesi, Indonesia
2Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Abstract
Vibriosis is a major concern in aquaculture causing rapid large amounts of fish death and economic loose. Thus, the new antibacterial compounds which are more specific and selective for Vibrio sp. are needed through the exploration of marine resources. The aim of this study to determine the chemical profiles of antivibrio compounds by mangrove sediment and marine sponge-associated bacteria. The mangrove sediment originated from the coastal of Situbondo, Cilacap, Rembang, and Palembang, while the sponges were collected from Manado. The secondary metabolites fermentation was carried out in various media, then the supernatants was tested for microtiter bioassay against V. alginolyticus, V. harveyi and V. parahaemolyticus in 96-well plate. Detection of bioactive compounds were done by Thin Layer Chromatography (TLC) with indicators of UV 254 nm and UV 366 nm, and TLC staining using ninhydrin, p-anisaldehyde, and H2SO4 reagents. The screening results showed that all the samples possessed bioactivity against Vibrio sp.. TLC results showed that the CIL-10-2016-01/02 and TPMD-13-01/02 were predicted to contain peptide compounds with pink discoloration on the TLC spot after dyeing with ninhydrin. The results of bioautography assay showed that isolates were active against V. harveyi and V. parahaemolyticus at Rf 0.16 and at Rf 0.57, respectively which indicated that the compound is a peptide molecule. H-NMR spectra indicated that there were peptide compounds in the combination bacterial isolates CIL-10-2016-01/02 and TPMD-13-01/02. Thus , the results showed that the combination bacterial isolates was capable to produce antivibrio compounds
