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181	Sustainable Aquaculture and Fisheries	ABS-126
Comparison of Minnow Trap Catches Using Peperek Fish Bait and Broiler Chicken Skin in the Seagrass Field Area of Parak Village, Bontomanai District, Selayar Islands Regency Andi Assir Marimba (*), Alfa Filep Nelwan, Mahfud Palo, Najamuddin and Nur Wahda Departement of Fisheries, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar, South Sulawesi, Indonesia 90245 *andiassirmarimba[at]gmail.com Abstract This study aims to obtain the type of bait that is effectively used in the operation of minnow traps, especially those used in seagrass beds. The feed criteria must correspond to the characteristics of the feed commonly used, namely aroma, durability and economic value. This research was conducted in October 2021-January 2022 located on the coastline of Parak Village, Bonto Manai District, Selayar Islands Regency. The method used in this study is a case study. Primary data collection is obtained directly by following, recording, identifying from the results of observations on the composition of types and comparisons of catches. During the study obtained 30 data. The data collected is the number of catches when using peperek fish bait and when using boiler chicken skin bait. The total number of catches when using peperek fish bait was 148 and the total number of catches when using boiler chicken skin bait was 123 consisting of 3 groups of catches, namely gastropods, fish and crustaceans. The composition of the most types when using peperek fish baits is Nassarius crematus as much as 31% and Nassarius coronatus 28% while the highest composition when using boiler chicken skin baits is in Nassarius crematus as much as 25% and Nassarius coronatus 25%. There is a significant comparison of minnow trap catches when using peperek fish bait and when using boiler chicken skin bait. Keywords: Catch- Minnow trap- Peperek fish and chicken skin baits- Selayar Islands Share Link | Plain Format | Corresponding Author (Andi Assir Marimba)

182	Sustainable Aquaculture and Fisheries	ABS-130
Growth performance of mud crab juveniles Scylla olivacea and S. tranquebarica in the brackishwater pond and their molting increment from immature to mature stages in captivity Muhammad N. Syafaat, Khor Waiho, Gunarto, Mhd Ikhwanuddin 1Higher Institution Center of Excelence (HiCOE) Institute of Tropical Aquaculture (AKUATROP), University Malaysia Terengganu (UMT), Kuala Nerus, Terengganu 2National Research and Innovation Agency (BRIN), Jakarta, Indonesia Abstract Mud crabs, Scylla olivacea (SO) and S.tranquebarica (ST) are frequently found in the same habitat in the river of estuaries, so both are viable candidates for cultivation in brackishwater ponds. The current study findings are expected to serve as the foundation for identifying species that are better suited for pond rearing based on growth performance criteria. This study tried to compare the growth performance of SO and ST crablet (carapace width 1cm) in the earthen pond and their female molting increment from immature to mature stages in captivity. The results showed ST had a better growth rate than SO based on the results of growth sampling in the first, second, and third months (P<0.05). The carapace width and average weight obtained in the third month for SO and ST were 82.82mm/103.46g and 90.57mm/131.03g respectively. The averages of the molting increment from immature to mature stages of SO and ST were 15.95% SD2.36 and 14.40% SD3.11 respectively, and the results did not show a significant difference (P>0.05). The results of this study can be considered when choosing a candidate species of mud crab that has a better growth performance in the brackishwater pond, namely S. tranquebarica although the percentage of molting increment of both species from immature to mature stages is same. Keywords: Mud crab juveniles, Growout, Aquaculture, Brackishwater pond, Molting increment Share Link | Plain Format | Corresponding Author (Muhammad Nur Syafaat)

183	Sustainable Aquaculture and Fisheries	ABS-131
Development and characterization of bio-degradable Annona-based film inclusion with chitosan Nur Shafinaz Abu Bakar (a), Kamariah Bakar (b), Nurul Ulfah Karim (a) (a) Institute of Tropical Aquaculture and Fisheries (b) Institute of Marine Biotechnology Abstract Biofilm is a biodegradable and sustainable packaging material. In contrast, synthetic packaging has raised serious environmental concerns by their non-biodegradability. The aim of this study is to develop a new natural-based biofilm made of Annona extract reinforced with chitosan. Annona-based biofilms were prepared using different concentrations of chitosan (0, 1, 2 and 4%). The effect from inclusion of different concentration of chitosan were evaluated using physical, mechanical, chemical and optical properties of the film. Thickness values for all biofilms showed significant difference (p<0.05) to each other. The film thickness was significant increase with increasing chitosan concentration due to compacity of film network. Biofilm without chitosan showed significant lower (p<0.05) moisture and elongation at break (EAB) compared to biofilm with 1% chitosan. Meanwhile, the opacity values of biofilm without chitosan showed significant lower (p<0.05) compared to biofilm with 4% chitosan. The films with higher chitosan were less transparent compared than the control due to the presence of more compounds in the film. Increasing chitosan concentration also resulted in lighter brown-yellowish color films as compared to deep brown control films. Overall, biofilm inclusion with Annona extract and chitosan has potential for natural biodegradable packaging material. Keywords: Annona, Chitosan, Biofilm Share Link | Plain Format | Corresponding Author (Nur Shafinaz Abu Bakar)

184	Sustainable Aquaculture and Fisheries	ABS-134
Impact of Covid-19 Pandemic on Tuna Small Scale Fishing in Indonesian Archipelagic Waters (IFMA 713, 714 and 715) Ignatius Trihargiyatno, Lilis Sadiyah, Fayakun Satria, Dian Novianto, Mahiswara and Agustinus Anung Widodo Research Center for Fishery, National Research and Innovation Agency Abstract COVID-19 pandemic has been believed to disrupt most economic sectors, including fishing industry. However, there are few information related to impact of the COVID-19 to tuna small scale fisheries in the Indonesia^s archipelagic waters (IAW) (Fisheries Management Areas 713, 714 and 715). In order to identify the impact of COVID-19 pandemic on tuna small scale fishing activities (pole and line, purse seine and Handline-Troll line) in the IAW, a study was conducted in two main tuna fishing ports in the area, i.e. Kendari (Southeast Sulawesi) and Bitung (North Sulawesi). The key aspects to be investigated are number of active vessels, number of landing or fishing trips, day at sea (DAS) or day of fishing (DOF), catch and nominal catch per unit effort (CPUE) for each tuna fishing gear which were collected for the period of 2019-2020. The results show that there is no clear pattern showing the changes on fishing operations of small scale tuna fisheries before and during COVID-19, suggesting there is no or little impact of COVID-19 on small scale tuna fishing operations in IAW. Keywords: Tuna, small scale, archipelagic Share Link | Plain Format | Corresponding Author (Lilis Sadiyah)

185	Sustainable Aquaculture and Fisheries	ABS-135
Survival, growth and feed efficiency of nile tilapia (Oreochromis niloticus) reared in groundwater containing high iron concentration of different salinitiesubmit This Sample Abstract Uras Tantulo University of Palangka Raya Abstract Keywords: Keywords: nile tilapia, salinity, groundwater, iron Share Link | Plain Format | Corresponding Author (Uras Tantulo)

186	Sustainable Aquaculture and Fisheries	ABS-139
Bottom Longline^s Productivity Operated in The Waters of Makarangana Island, Pangkep Regency Muhlis M (a), Alfa Nelwan (a*), Muhammad Kurnia(a) (a) Faculty of Marine and Fisheries sciences Jln. Perintis Kemerdekaan Km 10 Tamalanrea, Makassar, Indonesia Abstract The purpose of this study was to determine the species composition of the bottom longline^s catch and describe the frequency of occurrence of fish species based on fishing trips of the bottom longline. This research was conducted from February to March 2019 on Makarangana Island, Sabalana Village, Liukang Tangaya District, Pangkep Regency, South Sulawesi Province. The data collection method is used in the case study method by directly following the fishing operations of the bottom longline for 30 fishing trips. The results showed the composition of the type of fish dominantly caught with a longline that operates in the waters of Makarangana Island was giant trevally (Caranx ignobilis) 88.71 kg that was 20%, Crimson Snapper Fish (Lutjanus erythropterus) 71.91 kg that was 16%, Snout Lustam Fish (Snout Fish) (20%) Lethrinus microdon) 59.82 kg which was 13%. However, when viewed from the catch aspect, the production of fish species of the Letrinidae and Lutjanidae families were more than that of the giant trevally (Caranx ignobilis) species. The frequency of emergence of catch fish that was dominantly caught with a longline that operated in the waters of Makarangana Island was a snout fish species (Letrinus microdon) 53.3%, Crimson Snapper fish (Lutjanus erythropterus) 43.3% and Red Snapper (Lutjanus malabaricus) 40.0 %. The highest fishing productivity of the bottom longline operating in the waters of Makarangana Island was 0.13 fish/hook. Meanwhile, the lowest fishing productivity was 0.03 fish/hook. Keywords: bottom longline- species composition- frequency of occurrence- capture productivity Share Link | Plain Format | Corresponding Author (Alfa Nelwan)

187	Sustainable Aquaculture and Fisheries	ABS-140
Characterizing gut microbiota of adult and juvenile giant freshwater prawn Macrobrachium rosenbergii in Nyatoh River, Malaysia Sairatul Dahlianis Ishak (a*), Meng-Kiat Kuah (b), Victor Tosin Okomoda (a), Hon-Jung Liew (a), Rabi Atun Adawiyah Abdullah (a), Norainy Md Husin (a), Md Ikhwanuddin (a,c) (a) Higher Institution Centre of Excellence (HICOE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia (b) Lab-Ind Resource Sdn Bhd, 48300 Bandar Bukit Beruntung, Selangor, Malaysia (c) STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, Guangdong, China Abstract A survey was conducted at Nyatoh River, in the state of Terengganu, Malaysia, to evaluate the gut microbiome profile of the giant freshwater prawn (Macrobrachium rosenbergii) between two life stages, juvenile and adult. Sampling was done in a 5-day expedition and gut samples were collected and microbial communities were characterized using 16S rRNA amplicon sequencing on the Illumina platform. The difference in life stages had physiologically influenced gut microbiome. Moreso, the changes in external environment between locations might have also directly introduced environment-associated microbes, which indirectly interfered with the composition of indigenous gut bacteria. Keywords: aquaculture, freshwater prawn, gut microbes, health management, inland fisheries, Terengganu. Share Link | Plain Format | Corresponding Author (Sairatul Dahlianis Ishak)

188	Sustainable Aquaculture and Fisheries	ABS-142
Comparative degradability of polypropylene (PP) microplastics by two efficient floc-forming bacteria, Bacillus cereus and Bacillus infantis, isolated from a commercial aquafarm Shahadat Hossain1, Zuhayra Nasrin Ahmad Shukri1, Khor Waiho1, Yusof Shuaib Ibrahim2 Amyra Suryatie Kamaruzzan1, Ahmad Ideris Abdul Rahim1, Zahidul Islam3, Nor Azman Kasan*1,2 1Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. 2Microplastic Research Interest Group (MRIG), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 3Marine Fisheries and Technology Station, Bangladesh Fisheries Research Institute, Cox^s Bazar Sadar-4700, Cox^s Bazar, Bangladesh. *Corresponding author: Nor Azman Kasan, Higher Institution Centre of Excellent (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia. Email: norazman[at]umt.edu.my Abstract Microplastics pollution in aquaculture has got recent attention due to the wide usage trends of plastics in aquaculture operations. Microplastics especially polypropylene (PP), are one of the most commonly found MPs in the aquaculture environment in recent studies. A popular technology called biofloc technology (BFT) uses floc forming microorganisms to alleviate water pollutants in the aquaculture environment. The potentiality of the floc-forming bacteria in treating the MPs particles in water is rarely studied. This study aimed to investigate the comparative potentialities of two effective floc forming bacteria, Bacillus cereus, and B. infantis in degrading PP microplastics. The bacteria were isolated from a commercial aquafarm, and identified by using 16s rRNA sequencing. Floc forming ability was measured in kaolin clay suspension. After the incubation of the identified bacteria in mineral salt media for 60 days, the biodegradation ability was evaluated by measuring the weight loss of microplastics, optical bacterial growth, bacteria colony growth, microplastics surface characterization, and detecting alterations in chemical compositions by FTIR spectroscopy. B. cereus showed a higher flocculation activity, 93.66% than B. infantis (88.64%). Overall, B. cereus showed significantly higher degradability by showing higher bacterial colony growth, weight loss (4.61%), optical bacteria growth, microplastic surface changes, and changes in chemical composition. In conclusion, the study showed floc forming bacteria can degrade PP microplastics and B. cereus can be an effective microplastics degrader that can ensure a sustainable pollution free aquaculture system. Keywords: Microplastics pollution, polypropylene, biofloc technology, biodegradation ability, weight loss Share Link | Plain Format | Corresponding Author (MD. SHAHADAT HOSSAIN)

189	Sustainable Aquaculture and Fisheries	ABS-144
PPendugaan Allowable Biological Catch (ABC) dan Allowable Biological Effort (ABE) dengan aplikasi feedback harvest control rule (FHCR) Pada ikan demersal di wilayah pengelolaan perikanan replubik Indonesia (WPP-NRI)712lease Just Try to Submit This Sample Abstract PleTri Djoko Lelono, Gatut Bintoro, Ledhyane Ika Harlyan Sabrina Vanezza Putri2ase Just Try to Submit This Sample Abstract FPIK Universitas Brawijaya Abstract Perikanan demersal merupakan spesies yang paling diminati dan memiliki nilai jual yang tinggi serta diharapkan mampu memberikan pemasukan yang besar dibandingkan dengan jenis ikan lainnya Wilayah pengelolaan perikanan negara republik Indonesia (WPP-NRI) 712, khususnya pada perikanan demersal saat ini telah mengalami tangkap lebih (overfishing). Hal yang mendasari masalah tersebut ialah pada sistem pengelolaannya. Selama ini pendekatan yang digunakan dalam pengelolaan perikanan ialah model surplus produksi Schaefer. Model tersebut kurang tepat digunakan karena dirancang untuk perikanan spesies tunggal (single species). Oleh karena itu, strategi penangkapan Feedback Harvest Control Rule (HCR) dapat dijadikan alternatif dalam pengelolaan perikanan di Indonesia karena dapat digunakan bagi perikanan multispecies. Penelitian ini dilakukan untuk mengetahui perbandingan hasil dari kedua metode yaitu, model surplus produksi Schaefer dan Feedback Harvest Control Rule (HCR). Hasil analisis yang didapat, pada metode HCR didapatkan nilai ABC atau YJTB dan nilai ABE atau FJTB pada spesies ikan swanggi (Priacanthus tayenus) dan ikan kuniran (Upeneus sulphureus) lebih tinggi dibandingkan model surplus produksi Schaefer. Sedangkan metode HCR pada spesies ikan sebelah (Psettodes erumei) nilai ABC atau YJTB lebih rendah dari model surplus produksi Schaefer dan nilai ABE atau FJTB lebih tinggi dari model surplus produksi Schaefer. Keywords: Plikan kuniran, ikan swanggi (Priacanthus tayenus) , perikanan demersal, schaefer, HCRease Just Try to Submit This Sample Abstract Share Link | Plain Format | Corresponding Author (Tri Djoko Lelono)

190	Sustainable Aquaculture and Fisheries	ABS-149
The Utilization Different Size of Feed on Growth and Survival in Marine Worms (Nereis sp) Cultivation Sitti Faridah(a*), H Batubara (a), M Syaichudin (a), A Gafur (a), Hamzah (a), Akmal (a), Y Mundayana (a), I Sudrajat (a), Lideman (a), Haruna(b), N Hartanto (c) aResearch Center for Fishery, Research Organization for Earth Sciences and Maritime, National Research and Innovation Agency, Cibinong, West Java, Indonesia. bBrackishwater Aquaculture Development Centre, Takalar, South Sulawesi Province, 92254, Indonesia. cDirectorate General of Aquaculture, Ministry of Marine Affairs and Fisheries Republic of Indonesia. *sitt006[at]brin.go.id Abstract Sea worms have jaws equipped with teeth so that they can function to pick up and catch their food. Based on this, we consider it necessary to engineer several feed sizes used in the cultivation of marine worms (Nereis sp). This engineering aims to determine the growth and survival rate of sea worms (Nereis sp) reared by giving different feed sizes and to knowing the right size of feed for the maintenance of sea worms. The test animals used in this engineering are sea worms, which are used as much as 50 g in each engineering container. There are 3 sizes of feed used, namely feed size 0.1-0.9 mm = A, feed size 1.0-2.0 mm = B, and feed 2.1-3.0 mm = C with 3 repetitions. Engineering results show that the feed size is 2.1-3.0 mm better than the other feed sizes. Keywords: Feed, Nereis sp, Growth, and Survival Share Link | Plain Format | Corresponding Author (Sitti Faridah)

191	Sustainable Aquaculture and Fisheries	ABS-152
Temperature and Stocking Density at Early Developmental Stages Affects Growth, Gonadal Development, and Cannibalism Rate in The Juvenile Snakehead Munti Sarida (a,b*), Puput Ayu Nurvadlila (a), Aryanti Rafika Sari (a), Wawan Abdullah Setiawan(c,d), Muhammad Irsyad (e) a) Faculty of Agriculture, University of Lampung b) Postgraduate Program, Lampung University, c) Faculty of Mathematics and Science, University of Lampung d) Technical Services Unit Integrated Laboratory and Central Innovation Technology, University of Lampung e) Faculty of Engineering, University of Lampung Jl. Prof. Dr. Ir. Sumantri Brojonegoro No. 1, Rajabasa, Bandar Lampung, Lampung Abstract Slow growth, high mortality, and the unclear status of the reproductive pattern were several problems faced during the culture of snakehead. These problems could be influenced by environmental factors such as water temperature and stocking density. The purposes of this study were to evaluate those factors on the growth, cannibalism, sex ratio, survival rate, and hormonal level during an early stage of snakehead. The fishes were reared for 63 days at the interaction between temperatures of 24-25C, 28-29C, and 31-32C and stocking densities of 2 and 16 fish/l with two replication each. On rearing days 0, 7, 21, 35, 49, and 63, fish weight and length were measured to determine the absolute weight growth (AWG), specific growth rate (SGR), cannibalism, sex ratio, survival rate, and hormonal level. The results showed that the temperature of 28-29C was significantly different on the specific growth rate of 10.82%/day and blood glucose levels of snakehead juveniles were 119.45 mg/dL (P<0,05). The effect of stocking density of 16 fish/l was significantly different on blood glucose levels of snakehead juveniles 102.66 mg/dL (P<0,05). Further, there is no significantly different effect of treatments on both cannibalism and the survival rate of juvenile snakehead fish. On the other hand, there was a significantly different sex ratio in the percentage of males of juvenile snakeheads affected by the treatment. Moreover, there was positive interaction between temperature and stocking density on the specific growth rate which influenced snakehead juveniles. Keywords: Temperature and Stocking Density at Early Developmental Stages Affects Growth, Gonadal Development, and Cannibalism Rate in The Juvenile Snakehead Share Link | Plain Format | Corresponding Author (Munti Sarida Sarida)

192	Sustainable Aquaculture and Fisheries	ABS-155
Use of Garlic Vine, Mansoa alliacea (Lam.) Leaf Extract as Potential Antibacterial to Control Pathogenic Bacteria in White Leg Shrimp, Litopenaeus vannamei Arief Taslihan*, Endah Sutanti*, Suryati*, Zaenal Arifin*, Ahmad Fairus Mai Soni*, Sugeng Joko Purnomo* National Research and Innovation Agency Abstract The application of drugs in aquaculture has received serious attention from the WHO organization because it is feared that improper use will pollute the environment and cause bacterial resistance to antibiotics (AMR). It is necessary to do an alternative treatment using antimicrobial agents that are environmentally friendly and do not cause bacterial resistance. In this study, an effort looking for organic antimicrobials was from Garlic vine purple leaves. Garlic vine purple is known to have a bioactive substance such as aniline and other ingredients that function as antibacterial and fungal. The purpose of this study is to find out the effect of garlic vine purple leaf extract to kill pathogenic bacteria (Minimum Bactericidal Concentration, MBC) in shrimp. Extraction is done by harvesting garlic vine leaves, thinly sliced , then dried. Finely ground and tested for Minimum inhibitory concentration (MIC). Testing the effectiveness of the garlic vine leaf extract was carried out by treating shrimp with feed containing 2,5% and 5% garlic vine leaf extract. It can be concluded that garlic vine leaf extract has a MIC of 12.5% against Vibrio harveyi, V. parahaemolyticus originated from shrimp with White feces syndrome and Acute hepatopancreatic and necrosis disease. Further test, feed containing 5% extract of garlic vine leaf inhibited the growth of V. parahaemolyticus bacteria. The treatment of the purple garlic vine extract did not have a negative impact on shrimp survival in the 5% treatment and even gave better growth in length and biomass compared to no treatment with the purple garlic vine extract. Suggestions, further testing of the extract preparation system is needed, using nanotechnology. Treatment studies need to be carried out on a mass scale for the treatment of shrimp with WFS and AHPND at the shrimp farming scale. Keywords: garlic vine leaf extract, antimicrobial, white feces syndrome, acute hepatopancreatic necrosis disease Share Link | Plain Format | Corresponding Author (Arief Taslihan)

193	Sustainable Aquaculture and Fisheries	ABS-158
Evaluasi reduksi limbah pada feeding rate berbeda oleh organisme suspension feeder dan deposit feeder dalam sistem resirkulasi akuakultur multi-trofik Heriansah(a*), Radjuddin Syamsuddin(b) Najamuddin(c), Syafiuddin(d) 1 Institut Teknologi dan Bisnis Maritim Balik Diwa, Makassar, Indonesia * heriansah.itbm.bd#gmail.com 2 Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin, Makassar, Indonesia Abstract Limbah pakan monokultur intensif mengancam keberlanjutan produktivitas perairran. Mengurangi dampak tersebut menjadi tantangan utama dalam akuakultur. Tiga feeding rate (5%, 7,5%, dan 10%) ikan baronang dicobakan untuk mengetahui kemampuan reduksi limbah oleh suspension feeder (kerang hijau) dan deposit feeder (landak laut) di dalam sistem resirkulasi akuakultur multitrofik skala laboratorium. Pakan diaplikasikan empat kali sehari selama 90 hari pemeliharaan, Air media pemeliharaan pada setiap wadah berputar selama dua jam. Organisme suspension feeder dan deposit feeder berkontribusi mengurangi limbah nutrien dengan efisien. Limbah nutrien (NH4-N, NO3-N, dan PO4-P) yang meningkat oleh peningkatan feeding rate diredukasi oleh kemampuan kerang hijau dan landak laut memanfaatkan limbah tersebut. Konsentrasi NH4-N, NO3-N, dan PO4-P air media pemeliharaan kerang hijau tereduksi 51,21-97,61%, 82,97-87,30%, dan 51,21-79,92%, masing-masing pada feeding rate 5%, 7,5%, dan 10%). Sementara itu, reduksi oleh landak laut masing-masing 47,99-75,94%, 75,68-90,15%, dan 96,85-98,18% Keywords: feeding rate- kerang hijau- landak laut- reduksi limbah- resirkulasi- akuakultur multi-trofik Share Link | Plain Format | Corresponding Author (Heriansah Heriansah)

194	Sustainable Aquaculture and Fisheries	ABS-159
Nutrient waste removal efficiency of Seaweed Kappaphycus alvarezii by vertically method under different feeding rates in Integrated Multi-Trophic Aquaculture System Heriansah(a*), Radjuddin Syamsuddin(b) Najamuddin(b), Syafiuddin(b) a) Institute of Maritime Technology and Business, Balik Diwa, Makassar, Indonesia * heriansah.itbm.bd[at]gmail.com b) Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Abstract A study of three feeding rates (5%, 7.5%, and 10% day-1) for rabbit fish, Siganus guttatus) was tried in a multitropic aquacuture method with a closed water recirculation system in laboratory scale involving green mussels Perna viridis, sea urchins Diadema cytosum , and Kappaphycus alvarezii seaweed. aims to determine the ability of seaweed K. alvarezii in reducing the concentrations of ammonium (NH4-), nitrate (NO3-), and orthophosphate (PO4-) produced from feed waste released at each feeding rate that was dissolved in the seaweed water culture media. During the 90 day experiment the capacity of K. alvarezii cultivated by the vertical method reduced the concentration of nitrogen and phosphorus compounds was quite good, showing that this algae was effectively utilized in the management of fish feed waste in a multitrophic aquaculture system. The concentrations of ammonium (NH4-N), nitrate (NO3-N), and orthophosphate (PO4-P), reduced in the culture media of K. alvarezii at feeding rates of 5%, 7.5%, and 10% were 84.28-89.12% ammonium, 86.88-89.07% nitrate, 98.31- 98.74% phosphate- 82.70-90.69% ammonium, 87.19-92.50% nitrate dan 98.12-99.23% phosphate- and 87.85-92.25% ammonium, 99.31-99.23% nitrate , and 98.71-99.86% phosphates, respectively Keywords: feeding rates- Kappaphycus alvarezii- integrated multi-trophic- nutrient removal- recirculating system Share Link | Plain Format | Corresponding Author (Heriansah Heriansah)

195	Sustainable Aquaculture and Fisheries	ABS-161
The utilization of chamfer in fattening mangrove crab, Scylla olivacea consuming feed GEL containing various shrimp paste origins as attractants with system of vertical crab house and RAS Edison Saade, Zainuddin, Abustang and Reski Wahyuni Sukardi* UNIVERSITAS HASANUDDIN Abstract The price for mud crab cultivation with apartment technology or system of Vertical Crab House (VCH) is very expensive. One of the innovations to overcome this is to use chamfer. GEL feed is an artificial feed type semiwet containing sea weed, K. alvarezii as a thickening agent and a source of nutrition made by steaming. Shrimp paste can increase the attractiveness, feed consumption and weight gain of mangrove crabs. This research is an initial study to determine survival rate, feed consumption, feed efficiency, weight gain and condition factors for mud crabs reared in cahmfer with the Recirculating Aquaculture System (RAS) which are fed GEL from various different origins as an attractant with the VCH System or the system of apartment. The research was conducted at the Mini Hatchery of the Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, South Sulawesi. The test animals used were male mangrove crabs with an average weight of 108.45 10.23 g. Mud crabs are kept in a chamfer (size 100 x 13 x 11 cm) which is placed on a three-story iron rack. Each chamfer is partitioned in four using a wire net and each partition is filled with one crab. The study was designed using a completely randomized design (CRD) consisting of 4 treatments with 3 replications each, namely homemade shrimp paste called UNHAS product (treatment A), Malili shrimp paste (B), Selayar shrimp paste (C), and Kendari shrimp paste (D). Parameters measured were survival rate, feed consumption, daily relative growth rate, condition factor and feed efficiency. The results of the analysis of variance showed that the use of different shrimp paste sources had no significant effect (p>0.05) on survival, feed consumption, growth, feed efficiency and condition factors for mud crabs. Based on the results of this study it was concluded that all shrimp paste as an attractant and a source of nutrition could be supplemented in GEL feed for fattening mud crabs in chamfers with VCHS and RAS. Keywords: Attractant, fattening, gel feed, mangrove crab, Recirculating Aquaculture System (RAS) Share Link | Plain Format | Corresponding Author (RESKI WAHYUNI SUKARDI)

196	Sustainable Aquaculture and Fisheries	ABS-164
Apparent Digestibility of Distillery Yeast Sludge Meal for Juvenile of Red Tilapia (Oreochromis niloticus) Jacob L.A. Uktolseja1, Frendy Kurniawan1, Budhi Prasetyo2 1.Faculty of Biology, Satya Wacana Christian University, Salatiga, Indonesia 2. Faculty of Medicine and Health Sciences, Satya Wacana Christian University, Salatiga, Indonesia Abstract For sustainable aquaculture, distillery yeast sludge meal (DYSM) derived from molasses fermented by Saccharomyces contains nutrition that can replace fishmeal (FM). Therefore, this study evaluated the apparent digestibility coefficients (ADCs) of nutrients of red tilapia (Oreochromis niloticus) juveniles with DYSM as an ingredient of feed. Fish was fed four experimental diets, which contained DYSM and FM respectively of 0 and 30% (D1) as the positive control, 5 and 15% (D2), 10 and 15% (D3), 15 and 15% (D4)- 0 and 15% (D5) as the negative control, during 90 days. The results show there were increasing trends of ADCs of protein, lipid, ash, and dry weight as the level of DYSM increased from 5 to 15%. ADCs of the protein of D4 did not significantly differ (P>0.05) from that of D5. ADCs of lipid, carbohydrate, and dry weight of D4 were not significantly different from D1. ADCs of ash of D4 level were significantly higher (P<0.05) than all other diets. It is concluded that the addition of DYSM in the ingredients of red tilapia feed does not hamper the digestibility of nutrients- therefore, DYSM can be used as a standard ingredient of fish feed to replace partially fishmeal. Keywords: distillery yeast sludge meal, apparent digestibility, red tilapia Share Link | Plain Format | Corresponding Author (Jacob Lucas Alexander Uktolseja)

197	Sustainable Aquaculture and Fisheries	ABS-166
Application of Surplus Production Model to the Tuna (Thunnus Sp.) in Makassar Strait waters Muh. Aldhy Hatmar(a), Alfa Nelwan(a*), Musbir (a) (a) Jln. Perintis Kemerdekaan Km 10, Faculty of Marine and fisheries Sciences, Universitas of Hasanuddin, Makassar City, South Sulawesi Abstract Tuna (Thunnus Sp.) is one of pelagic fish that has high potential and economic value in Makassar strait. Utilization of this resource is using Handline tuna units, with the number of handline continuously increasing. Therefore, management needs to be done so that optimal productivity can be maintained. This study discusses the estimation of catch and effort at maximum sustainable yield (MSY) of Tuna (yellowfin and Big Eye) based on catch per unit effort (CPUE) and handline production in Majene Regency, West Sulawesi Province during 2007-2022. Mathematical analysis was carried out using the equilibrium approach with the Schaefer model. The highest catch of Tuna reached 143.47 ton in July 2022 (yellowfin tuna reached 84.67 ton and Big Eye Tuna reached 58.8 ton). The average CPUE for tuna was 4.7 tons/unit with CMSY of 1113.99942 tons/year and EMSY of 128 unit/year. From 2014,2015,2019 and 2020, the trend of biomass continued to decline and overfishing occurred during this period. Keywords: CPUE, MSY, Tuna, Surplus Production Model Share Link | Plain Format | Corresponding Author (Alfa Nelwan)

198	Sustainable Aquaculture and Fisheries	ABS-167
The Effect of Immersion Extract Viscera of Sea Cucumber (Holothuria sp) on Growth, Consumption Level and Feed Efficiency in Tilapia Larvae (Oreochromis niloticus) Andi Aliah Hidayani1*, Marlina Achmad1, Alifah Nurul Jannati2, Atira Rewa2, Archangela Ghiriani Gareso2, A. Dyar Fadya Auliah2, Jitima Suwanmala3 1Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Tamalanrea, Makassar, South Sulawesi Indonesia. 2Aquaculture study program students, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, South Sulawesi 90245, Indonesia. 3Faculty of Sciences and Technology, Prince of Songkla University, Pattani Campus, Thailand Abstract The use of synthetic steroid hormones to produce quality male tilapia has a negative impact on both the environment and society, so alternative natural ingredients are needed such as sea cucumber innards. The purpose of this study was to determine the effect of sea cucumber innards extract on growth, consumption and feed efficiency of tilapia larvae. This research was carried out in September - November 2022. There were 360 larvaes of tilapia used in this study. Tilapia rearing was carried out in in each container containing 30 larvae with a larval weight of 0.013 gr and a larval length of 7 mm. The tilapia used were 5 to 7 days old, then larval rearing was carried out for 60 days. The experimental method used in this study was a Completely Randomized Design (CRD) with five treatments and three replications. The treatment used was the immersion of sea cucumber viscera with 5 treatments consisting of 0 ml, 1 ml for 24 hours, 3 ml for 24 hours, 5 ml for 24 hours, and 0,5 mg/l 17&#945--MT for 12 hours as positive control and 3 replicates. Parameters observed were growth performance, feed consumption, feed efficiency level and water quality. The results showed that soaking sea cucumber viscera at various doses for 24 hours in tilapia larvae has the potential to increase feed consumption which will ultimately affect growth. Doses of 1 ml and 3 ml are doses that change the absolute length growth results and feed consumption rates. Keywords: Sea cucumber viscera, Growth performance, Feed consumption, Feed efficiency level, larvae of tilapia Share Link | Plain Format | Corresponding Author (Andi Aliah Hidayani)

199	Sustainable Aquaculture and Fisheries	ABS-168
Innate Immunity Activation of Tilapia (Oreochromis niloticus) Against Vibriosis Disease Based On Protein Adoption of Sago Caterpillar Marlina Achmad1,2*, Andi Aliah Hidayani1, Sriwulan Sriwulan1, Adnan Teddy Syach2, Andi Muh. Fajrin R.F.2, Dewi Dian Anggraini2, Dedy Muhammad Iqbal2, Muhammad Fariz Zahir Ali3 1 Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University. Jl. Perintis Kemerdekaan KM. 10, Makassar, South Sulawesi, 90245,Indonesia 2 Vocational Faculty, Hasanuddin University. Jl. Perintis Kemerdekaan KM. 10, Makassar, South Sulawesi, 90245,Indonesia 3 Bachelor of Aquaculture Programme, Faculty of Marine Science and Fisheries, Hasanuddin University, South Sulawesi, 90245,Indonesia 3 Graduate School of Agriculture, Ehime University, 3-5-7, Tarumi, Matsuyama, Ehime, 790-8566, Japan Abstract Vibrio sp. is a widely distributed bacteria species responsible for vibriosis and a high mortality rate in fish. Therefore, it is necessary to prevent bacterial infection by enhancing the fish innate immune system response through the dietary inclusion of immunostimulants. One of the alternative immunostimulants from animal extracts is sago caterpillar meal (Rhynchophorus ferrugineus). This study analyzes the effects of dietary sago caterpillar meal on growth performance and innate immune response of tilapia (Oreochromis niloticus) after being infected with Vibrio harveyi. It was carried out using a completely randomized design with 4 treatments and 3 repetitions. Tilapia were fed diets containing sago caterpillar at a concentration of 0% (control), 10%, 20%, and 30%, respectively, for 30 days. Furthermore, a challenge test was conducted, followed by an immune response analysis by counting the total leukocytes and bacteria concentration found in fish gills. Growth performance analysis was also carried out by calculating daily weight and length gain, specific growth rate, feed conversion ratio, and fish survival. The results indicated the dietary inclusion of sago caterpillar meal affects total leukocyte count, over gill concentration, daily weight growth, specific growth rate, and survival. However, it did not affect daily length growth and feed conversion ratio in tilapia. The final conclusion is then obtained that dietary sago caterpillar meal at a concentration of 20% has the ability to increase the immune system against bacterial attack and induce the highest growth performance in tilapia. Keywords: Oreochromis niloticus, sago caterpillar, immune response, vibriosis, growth performance Share Link | Plain Format | Corresponding Author (Andi Aliah Hidayani)

200	Sustainable Aquaculture and Fisheries	ABS-169
Optimizing the Potential of Seaweed as Economic Strengthening for Coastal Communities in Majene Regency N I S Arbit(1*), F Syamsudin(2), M Thabrani(3), S R Carong(1), Erwin(1), D Lestari(1), D C Pratama(4) 1)Sulawesi Barat University, Majene, Indonesian *indaharbit[at]unsulbar.ac.id 2)National Research and Innovation Agency, Jakarta, Indonesian 3)Marine and Fisheries Office Majene Regency, Majene, Indonesian 4)Conservation Strategy Fund (CSF), Jakarta, Indonesian Abstract Seaweed is one of the leading fishery products with economic value and very promising business opportunities that can improve the welfare of coastal communities. The title of this research is Optimizing the Potential of Seaweed as Economic Strengthening for Coastal Communities in Majene Regency. The purpose of this study is to examine the potential of seaweed in Majene Regency, to analyze the suitability of cultivation land in Majene Regency, to analyze the success factors for seaweed cultivators around Majene Regency. Methods of data collection with geospatial data and information, mapping of seaweed cultivation, direct surveys and interviews. Data analysis used water quality analysis, land suitability analysis and AHP analysis. The results of measuring water quality parameters obtained were an average temperature of 30.25 0C, salinity 31.57 ppt, pH 5.81, dissolved oxygen 5.05 ppm, current speed 0.06 m/s, wave height 49.96 cm, ammonia 0.0011 ppm, nitrate 0.0236 ppm, nitrite 0.0278 ppm , and fofat 0.0252 ppm., Natural seaweed that grows in these waters. The quality of the water obtained supports the viability of seaweed. Results AHP farmers choose technology, AHP Government chooses human resources. Keywords: Economy, Majene, Seaweed, Water Quality Share Link | Plain Format | Corresponding Author (Nur Indah Sari Arbit)

201	Sustainable Aquaculture and Fisheries	ABS-171
Fisheries Status and Strategic Management Plan of Mud Crab (Scylla sp.) Based on the Ecosystem Approach to Fisheries Management Method in the Indonesian Fisheries Management Area Muhammad Syahdan (a), Muhammad Ahsin Rifa^i (a), Yushinta Fujaya (b), Nursalam (a*), Ira Puspita Dewi (a), Muhammad Lukman (b), Aris Budiarto (c), Muhammad Anas (c) a) Faculty of Fisheries and Marine, University of Lambung Mangkurat, Banjarbaru, Indonesia Jl. Jend. A. Yani Km. 36 Banjarbaru, Indonesia *nursalam[at]ulm.ac.id b) Faculty of Marine Science and Fisheries University of Hasanuddin, Makassar, Indonesia Jl. Perintis Kemerdekaan Km. 10 Tamalanrea Makassar, Indonesia c) Directorate General of Capture Fisheries, Ministry of Maritime Affairs and Fisheries of the Republic of Indonesia, Jakarta, Indonesia Jl. Medan Merdeka Timur No. 16 Jakarta Pusat, Indonesia Abstract The existence of mangrove crabs as a fish resource with crucial economic value and conditions that tend to experience ecological pressure requires a strategic basis for management. Based on the EAFM method, the measurement parameters consist of four management components, namely fish resources, environment, social economy and policy. Data collection to determine the status of mud crabs was carried out through a questionnaire method, searching fisheries databases from relevant agencies and literature studies. Strategic recommendations are formulated through Focus Group Discussions (FGD) of fisheries stakeholders from all provinces in Indonesia and in-depth interviews with related elements at the Ministry of Maritime Affairs and Fisheries-Republic of Indonesia (KKP-RI). The results showed that the production of mud crabs in 2017 - 2021 tends to decrease, and the category of utilization rate in WPP RI is divided into three status groups: moderate, fully exploited and overexploited. The relationship between mangrove density conditions and mangrove crab production shows that medium-density levels are more likely to produce high production than dense or rare densities. The economic impact of mangrove crab resources has a significant value in improving the welfare of fishermen, as indicated by the average fisherman exchange rate, which tends to be above the Provincial Minimum Wage in each province in Indonesia. Mud crab exports have also increased for the period 2019 - 2021. Strategic recommendations for management are focused on 4 (four) main issues, namely setting appropriate quotas and sizes for fishing mud crabs, conservation of mangrove habitat, coordination in monitoring marketing at local and export levels mud crabs, and institutional strengthening of stakeholder actors managing mud crabs. Keywords: mud crab- EAFM method- fisheries status- strategic management plan Share Link | Plain Format | Corresponding Author (Muhammad Syahdan)

202	Sustainable Aquaculture and Fisheries	ABS-174
HEA Visual Analysis for the Mangrove Rehabilitation Compensation Area in Bunati Village, Tanah Bumbu Regency Nursalam (a*), Ira Puspita Dewi (a), Siti Fatimah (a) a) Faculty of Fisheries and Marine, University of Lambung Mangkurat, Banjarbaru, Indonesia Jl. Jend. A. Yani Km. 36 Banjarbaru, Indonesia *nursalam[at]ulm.ac.id Abstract Mangrove is a forest that grows on the coast, generally found in protected estuaries, river banks, and lagoons in subtropical and tropical regions. Mangroves describe ecosystems and plant families that can adapt to an environment influenced by tides. The condition of the mangroves in Bunati Village is quite apprehensive due to the conversion of mangrove land to other land uses such as oil palm plantations, mining industrial excavations and others, seen from Google Earth images for 2013 and 2022, causing mangrove land cover in Bunati Village to decrease. Therefore it is necessary to analyze the compensation for mangrove rehabilitation areas in Bunati Village, Angsana District, Tanah Bumbu Regency, South Kalimantan Province. This study uses Sentinel 2 Satellite Imagery data for 2022, SPOT 7 for 2017, and SPOT 4 for 2012. The data analysis carried out overlays and Visual Habitat Equivalency Analysis. The area of mangroves that have decreased in Bunati Village is 73.78 hectares. Based on the total area, it is estimated that the compensation area for mangrove rehabilitation in Bunati Village with a 15-year rehabilitation scenario is 97.275 hectares. Keywords: mangrove- compensation area- rehabilitation- Bunati Village Share Link | Plain Format | Corresponding Author (Nursalam Nursalam)

203	Sustainable Aquaculture and Fisheries	ABS-175
The use of Cypress and Pine Flowers as Biofilter Media for Degrading Ammonia, Nitrites, and Nitrates in Comet Fish Rearing Badraeni, Syafiuddin and A. Ikhwan Department of Fisheries, Faculty of Marine Sciences and Fisheries, Hasanuddin University Abstract Abstract. The comet Carassius auratus is a type of fish that is quite popular as an aquarium ornamental fish at home and can be spawned naturally. To get good comet fish fingerlings, it is necessary to feed it with high protein, but it has an impact on increasing the concentration of ammonia in the rearing medium. Application of recirculation systems and biofilters can degrade ammonia. This study aims to analyze the utilization of Pine Flowers and Cypress Flowers as biofilter media to degrade ammonia, nitrate and nitrite in Comet C. Auratus fish farming. The research was carried out on a laboratory scale using plastic bottles as filtration media containers containing Pine Flowers, Cypress Winds and Bioballs, as well as cotton filters to filter leftover feed and faeces before entering the reactor. Giving pellet feed with a dose of 5% of the weight of the biomass. The results showed that the Pine Flower Biofilter was the best biofilter of other biofilters, only separating 0.049 ppm NH3, 0.162 ppm NO2 and 3.309 ppm NO3 in cultivation media compared to 0.101 ppm NH3 bioball, 0.487 ppm NO2 and 3.107 ppm NO3 and 0.110 ppm NH3 pine wind. NO2 0.426 ppm and NO3 3.149 ppm. The biofilter used showed no difference in growth, survival and NO3 content. Keywords: Biofilter Media, Comet Fish (C. auratus), Pine Flowers, Pine Flowers, NH3, NO2, NO3 Share Link | Plain Format | Corresponding Author (Badraeni Badraeni)

204	Sustainable Aquaculture and Fisheries	ABS-182
The Utilization of cassava dregs fermentation to accelerate biofloc formation in the white shrimp (Litopenaeus vannamei) culture M Syaichudin (a*), Rahmi (b), Akmal (a), H Batubara (a), Y Mundayana (a), A Gafur (a), Hamzah (a), Faridah S (a), Lideman (a), Jumriadi (c), Supito (d), Sadat (c), S Sujaka (c), I Sudrajat (a), Arfandi (a) (a) Research Center for Fishery, Research Organization for Earth Sciences and Maritime, National Research and Innovation Agency, Cibinong, Bogor 16911, Indonesia. (b) Aquaculture Study Program, Faculty of Agriculture, University of Muhammadiyah Makassar, Makassar 90221, Indonesia (c) Brackishwater Aquaculture Development Centre, Takalar 92254, Indonesia (d) The Main Centre For Brackishwater Aquaculture Development, Jepara 59418, Indonesia Abstract Cassava dregs is a byproduct of processing cassava into tapioca, through fermentation the carbohydrate structure will be simpler and possible as an essential carbon source. This resaerch aims to examine the utilization of cassava dregs fermentation/CDF in accelerating of the biofloc formation in white shrimp (Litopenaeus vannamei) culture. This research was carried out in two plastic ponds with 900 m2, where in treatment A with CDF and 200 indv/m2 and B without CDF and 300 indv/m2 for 60 days observation. Result of this research, the CDF in treatment A can accelerate the formation of biofloc at DOC 26 meanwhile in treatmen B at DOC 38. The weight growth at treatment A averaged 6.21g/indv while B averaged 6.21 g/indv. The Survival Rate/SR was significantly different, where in A was 99.1% and B was 75.3%. The SR level in A is thought to be due to the faster formation of biofloc. Feed convertion ratio respectively in treatment A and B were 1.4 and 1.49, with a total biomass of A 1,153 kg and B 1.263 kg. The final conclusion can be stated that the application of CDF in Biofloc Technology can increase the shrimp culture productivity. Keywords: cassava dregs fermentation, biofloc technology, shrimp culture Share Link | Plain Format | Corresponding Author (Mohammad Syaichudin Syaichudin)

205	Sustainable Aquaculture and Fisheries	ABS-183
Effects of fermented rain tree seed flour in feed, on chemical composition, glycogen content of the body and the growth of juvenile white shrimp Litopenaeus vannamei Asni Anwar(a,c*),Zainuddin(b), Muhammad Iqbal Djawad(b) dan Siti Aslamyah(b) a,c)Doctoral Study Program in Fisheries Science, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, South Sulawesi, Indonesia a,c* : asni[at]unismuh.ac.id b)Aquaculture Study Program, Department of Fisheries Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar 90245 Indonesia c)Aquaculture Study Program Faculty of Agriculture, University of Muhammadiyah Makassar, South Sulawesi, Makassar 90211, Indonesia Abstract The research aims to determine the level of soybean flour (SBM) substitution with rain tree seed flour (RTSF) fermented with mixed microorganisms in feed on body chemical composition, glycogen content, and growth of white shrimp. White shrimp with an initial weight of 0.08 g/shrimp are kept with a density of 40 heads in an aquarium measuring 50x30x35 cm3, 40 l in volume, containing 25 ppt salinity water and equipped with a recirculation system. The study was designed in a complete randomized design, with five treatments repeated three times, with different substitution rates, namely 0, 25, 50, 75, and 100%. During the 60 days of rearing, shrimp were given experimental feed 4 times a day at 06.00, 12.00, 18.00 and 21.00 in an amount of 10% of body weight. The results of the analysis showed that the treatment had a significant effect on crude protein levels, crude fat, Nitrogen Free Extract (NFE), glycogen content, and absolute growth of white shrimp, except for ash and crude fiber content. The highest protein content, fat, NFE, shrimp body glycogen content and absolute growth were obtained at the 50% substitution rate treatment and the lowest at the 0%. The crude fiber content ash were highest at the 0% substitution rate, and crude fiber ash were lowest at the 50% substitution rate. The substitution rate of soybean flour with fermented rain tree seed flour is well-tared in feed, to support the sustainability of white shrimp farming, with a ratio of 50% SBM: 50% RTSF Keywords: Feed, Litopenaeus vannamei ,Rain tree, Substitution. Share Link | Plain Format | Corresponding Author (Asni Anwar)

206	Sustainable Aquaculture and Fisheries	ABS-184
The Effect of Yellow and Blue Light Colors on the Size of Dominant Fish Catch of Fixed Lift Net in Pangkep Waters Muhammad Fadhli Tawil (a,b), Muhammad Kurnia (a*), Mukti Zainuddin (a), Ilham Jaya (a), Muhammad Ikhsan Amir (a), Adam (c) a) Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, 90245, Indonesia *kurniamuhammad[at]fisheries.unhas.ac.id b) Agriculture and Animal Husbandry Agency, North Kolaka, 93911, Indonesia c) Pangkep State Polytechnic of Agriculture, Pangkajene and Islands, 90655, Indonesia Abstract Fixed lift net has developed a lot, fishermen have begun to apply their fishing gear with different colors of lights. However, to determine the effect of color of the lamp, it is seen the size of one of the dominant catch species, namely Sardine (Sardinella sp). The study was conducted from August to November 2021. Experimental fishing research method, using T Test, Paired-samples T Test to determine the effect of lamp light color on fish size. The observation parameter is the size of the length of the fish crow. The total number of measurement samples was 1,440 Sardine with a total number of 32 trips of fishing. The results showed the heaviest total catch at yellow light rather than blue light. The average length of the Sardine obtained, the yellow light is 10.48 cm while the blue light is 8.70 cm. Yellow light gets the size of the fish larger than blue light. The test results stated the influence of lamp color on fish size, it was known that the value of Sig. (2 tailed) was 0.000 < 0.05, it was concluded that there was a real difference between the capture of yellow and blue lights. Keywords: Fixed lift net- light- color- length- sardine Share Link | Plain Format | Corresponding Author (Muh Fadhli Tawil)

207	Sustainable Aquaculture and Fisheries	ABS-187
Analisis Diameter dan Tipe Pertumbuhan Diadema setosum di Pulau Kodingareng Lompo, Makassar 1 Sharifuddin A. Omar 1 Wilma Moka 1Muh. Iqram Effendy 1 Basse S Parawansa 1 Suwarni 1) Aquatic Resource Management Study Program, Fac. of Marine Science and Fisheries, Universitas Hasanuddin Abstract Analysis of Diameter and Growth Type of Diadema setosum on Kodingareng Lompo Island, Makassar Wilma Moka 1Muh. Iqram Effendy 1 Basse S Parawansa 1 Suwarni 1) Aquatic Resource Management Study Program, Fac. of Marine Science and Fisheries, Hasanuddin University Abstract This study aims to analyze the diameter, size and growth type of sea urchins (Diadema setosum) on the islands of Kodingareng Lompo and Kodingareng Keke, Makassar City, South Sulawesi Province. This research was conducted for two months, from August to September 2022 on Kodingareng Lompo and Kodingareng Keke Islands, Makassar City, South Sulawesi Province. The number of sea urchins caught during the study was 1206 in August and 1066 in September. Sample analysis of sea urchins was carried out in situ on the island. Data analysis to determine the size distribution was carried out using a quantitative descriptive approach, including body diameter measurements. Based on the time of sampling in August, 12 classes were obtained with a mean size of 37.28 mm and a standard deviation of 14.34 mm with a total catch of 1206 individuals. In September, 11 classes were obtained with a mean size of 35.14 mm and a standard deviation of 13.62 mm with a total catch of 1066 individuals. The growth pattern of D.setosum sea urchins based on sampling time and station on Kodingareng Keke and Kodingareng Lompo Islands is negative allometric, namely the increase in shell diameter is faster than the increase in weight. (Approx. 191 words) Keywords: Growth Types, Diadema setosum, Kodingareng Lompo Topic: Sustainable Aquaculture and Fisheries Keywords: Growth Type, Diadema setosum, Kodingareng Lompo Share Link | Plain Format | Corresponding Author (Wilma Moka)

208	Sustainable Aquaculture and Fisheries	ABS-188
Effectiveness of Red Fruit Oil Against Resistance of Tilapia Exposed to Streptococcus Bacteria Marlina Achmad (a*), Andi Aliah Hidayani (a), Bunga Rante Tampangallo (b), Kamaruddin (b), Badraeni (a), Firdha Annisa Darmawan (c), A. Dyar Fadya Auliyah (c), Nursyamsi (c) (a)Fisheries Department, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, South Sulawesi, 90245. Indonesia *marlina.achmad[at]unhas.ac.id (b) National Research and Innovation Agency (c) Aquaculture Study Program, Fisheries Department, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, South Sulawesi, 90245. Indonesia Abstract Tilapia (Oreochromis niloticus) is a fishery commodity that has high value compared to other types of fish. The problem that often arises in the cultivation process is the presence of bacterial or pathogenic infections that can inhibit fish growth, reduce body resistance, thereby reducing tilapia production. One of the bacteria that often attacks tilapia is Streptococcus bacteria, with clinical symptoms that fish often cause are slowed fish movements, changes in fish skin color and death. Red fruit contains several active compounds with quite high levels, including tocopherols. Tocopherol content has activity as an antioxidant that can fight bacteria. The purpose of this study was to determine the optimum dosage of bitter melon oil to increase the resistance of tilapia to Streptococcus bacteria. The research will begin by making fruit oil extract treatments namely 0%, 1%, 3%, 5% and 10%. Furthermore, each dose was added to the test feed, and given to the test animals, namely tilapia measuring 10.5-16.5 g at a density of 10 fish/aquarium. Fish kept for 30 days. Next, the tilapia was exposed by immersing it for 30 minutes in a medium that had been treated with Streptococcus 107 cfu/ml. After that, observed the behavior of fish that had been exposed to bacteria for 4 days after immersion. Parameters observed were survival, daily growth rate, feed efficiency, fish hematology, erythrocyte count, hemoglobin count, hematocrit count, leukocyte count, leukocyte differential, phagocytic index, and water quality. The results showed that the leukocyte differentiation of tilapia after exposure to bacteria ranged from 1-14%, and treatment E produced the highest differential value. Furthermore, for leukocytes and erythrocytes, the highest were shown in treatment E, namely 43,650 cells/m3 and 38,200 cells/ml3, and treatment D, namely 4.51x106 cells/mm3 and 3.06x106 cells/mm3. Keywords: tilapia, Red fruit, bacterial disease, streptococcus Share Link | Plain Format | Corresponding Author (Marlina Achmad)

209	Sustainable Aquaculture and Fisheries	ABS-189
PREVALENCE AND INTENSITY OF ECTOPARASITES ON FARMED TILAPIA IN SARAWAK REGION, MALAYSIA, AND THEIR RELATIONSHIP WITH THE WATER QUALITY STATUS Salwati Baharudin (1), Sharifah Raina Manaf (1), Arif Azizi Che Harun (1), Nur Asha Shahira Zamri (1), Mohd Ihwan Zakariah (2), Wahidah Wahab (2), Jamaluddin Fitrah Alam (3), Kurniati Umrah Nur (3) (1) Faculty of Plantation and Agrotechnology, Universiti Teknologi Mara UiTM Cawangan Sarawak Kampus Mukah (2) Higher Institution Centre of Excellence, Institute of Tropical and Aquaculture and Fisheries (AKUATROP), University Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, Malaysia. (3) Faculty of Marine Science and Fisheries, Universitas Hasanuddin (UNHAS), Jl. Perintis Kemerdekaan, Km.10 Tamalanrea, Makassar, Sulawesi Selatan, Indonesia. Abstract A parasitological analysis on farmed tilapia (Oreochromis sp.) from the Sarawak region, involving Sri Aman, Miri, Mukah, and Sibu districts, was performed in 2022 and 2023. The prevalence and mean intensity were investigated for a total of 120 tilapia obtained from four distinct farms. In this study, all the tilapia specimens were infected with at least one ectoparasite species, showing a 100% prevalence rate of ectoparasite infestation. Six species of protozoans (Trichodina sp., Ichthyopthirius multifiliis, Ichthyobodo sp., Oodonium sp., Epistylis sp., and Ciliates sp.) and two species of monogeneans (Dactylogyrus sp. and Gyrodactylus sp.) were observed. The most intense ectoparasites recorded were Ciliates sp., Trichodina sp., and Dactylogyrus sp. Trichodina sp. and Dactylogyrus sp. were recorded higher in tilapia collected from Sri Aman district, while Ciliates sp. infected the tilapia from Miri district the most. The lowest intensity rates were recorded for Ichthyobodo sp. and Oodinium sp. where Ichthyobodo sp. was only observed on tilapia from Mukah district while Oodinium sp. was only recorded in fish from Miri district. These findings showed that the presence of parasites on fish is significantly influenced by the quality of the water. The information provided might be useful to the fish farmers in understanding the dynamics of tilapia parasites in the relevant area. Keywords: prevalence, intensity, ectoparasites, farmed tilapia, protozoans, monogeneans, water quality Share Link | Plain Format | Corresponding Author (Salwati Baharudin)

210	Sustainable Aquaculture and Fisheries	ABS-195
Absorption of nutrients on thallus of Kappaphycus alvarezii which is attached by epiphyte (Enteromorpha clathrata) Alimusra, Salwiyah, M Kasim* Faculty of Fisheries and Marine Science, Halu Oleo University Abstract Epiphyte attachment can cause competition in the absorption of nutrients for the seaweed thallus. This study aims to determine the relationship between growth rate and absorption of nutrients (N and P) in the thallus of Kappaphycus alvarezii attached by epiphyte (Enteromorpha clathrata). Nutrients have an important role for K. alvarezii. The research method used is data collection, including- daily growth of K. alvarezii, nitrogen uptake by the thallus of K. alvarezii and water quality parameters. Collecting nitrogen absorption data using the Kjedhal method. Environmental parameters were measured at each station together with the collection of K. alvarezii samples. The results showed that the growth of K. alvarezii which was attached by epiphyte (E. clathrata) had the highest value occurring on the 30th day with an average of 2.908-2.943%/day. The growth of the thallus that was not attached by the epiphyte showed the highest growth rate on the 30th day with an average of 3.502-3.522%/day. The total nitrogen uptake of K. alvarezii which was attached to the epiphyte (E. clathrata) reached 1.884% while that which was not attached reached 1.866%. The growth of K. alvarezii which was attached by Epiphyte (E. clathrata) was slow, while the thallus which was not attached by epiphyte experienced very good growth. Nitrogen absorption by K. alvarezi thalus showed no significant difference between the thalus attached and not attached to E. clathrata. Keywords: seaweed, growth rate, Nitrate, Phosphate Share Link | Plain Format | Corresponding Author (Maruf Kasim)

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