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151	Sustainable Aquaculture and Fisheries	ABS-47
The Effects of Dietary Lipid Level on The Growth Performance, Body Composition and Feed Utilization of Juvenile Kelabau (Osteochilus Melanoplueura) Adi Susanto (a*), Heru Kusdianto(a), Andi Nikhlani(a), Komsanah Sukarti(a), Kurdiansyah(a), Dani Febrian(a), Akhmad Syafei Sidik(b), Ismail Fahmy Almadi(c) a) Laboratory of Fish Nutrition, Departement of Aquaculture, Faculty of Fisheries and Marine Science, Mulawarman University, Samarinda, East Kalimantan, Indonesia *adisusanto[at]fpik.unmul.ac.id b) Laboratory of System and Technology Aquaculture, Departement of Aquaculture, Faculty of Fisheries and Marine Science, Mulawarman University, Samarinda, East Kalimantan, Indonesia c) Intregrated Laboratory, Departement of Aquaculture, Faculty of Fisheries and Marine Science, Mulawarman University, Samarinda, East Kalimantan, Indonesia Abstract This study was conducted to evaluate the effect of feeding with different lipid content on growth performance and body composition in Kelabau (Ostechilus melanopleura Bleeker). Four experimental diets isonitrogen, isoCHO and different lipid levels. A, B, C and D feeds contained 5%, 7%, 9% and 11% lipid levels respectively. Kelabau obtained from the cultivation in Balai Benih Air Tawar Mandiangin with an average weight of 3.18 g with a density of 20 individuals maintained in plastic tubes measuring 54.3 cm x 38 cm x 31.5 cm and filled 40 liters of water. Kelabau fed 2 times a day on at satiation for 60 days. The result showed that fish fed diet B (7%) had higher growth performance and feed utilization compare the other groups (C,D and A) (P <0.05). The increase in lipid administration in Kelabau from 5% to 7% have an effect protein, lipid and energy retention. Keywords: Growth Performance, Feed Utilization, Retention, Osteochilus melanopleura Share Link | Plain Format | Corresponding Author (Adi Susanto)

152	Sustainable Aquaculture and Fisheries	ABS-49
Komposisi Hasil Tangkapan dan Biologi Rajungan (Portunus pelagicus) pada Alat Penangkapan Ikan Jaring Insang di Ujungpangkah, Gresik, Jawa Timur Muhammad Arif Rahman1,2, Ledhyane Ika Harlyan1,2, Mihrobi Khulwatu Rihmi1,3, Wiaam Fat Al-fatih1 1Pemanfaatan Sumberdaya Perikanan, Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya 2MEXMA Research Group, Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya 3IM FISHER Research Group, Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya Abstract Jaring insang (gill net) merupakan salah satu alat tangkap pasif yang digunakan oleh nelayan untuk menangkap rajungan. Bersama dengan lobster dan kepiting, rajungan menjadi sumberdaya yang penangkapannya diatur oleh Peraturan Menteri. Penelitian ini memiliki tujuan untuk mengetahui komposisi hasil tangkapan jaring insang, biologi rajungan yang tertangkap, serta ketersesuaiannya dengan peraturan Menteri yang berlaku. Hasil penelitian menunjukkan bahwa terdapat 12 jenis sumberdaya ikan yang tertangkap, dengan 7 persen dari hasil tangkapan adalah rajungan. Dari 1647 ekor sampel, didapatkan bahwa hubungan Panjang berat rajungan adalah alometrik negatif, nisbah kelamin jantan:betina adalah 1:1. Rajungan betina (877 ekor) memiliki persentase Tingkat Kematangan Gonad (TKG) 1, 2, dan 3 sebesar 49%, 34%, dan 17%. Sementara itu, merujuk pada Peraturan Menteri Kelautan dan Perikanan (PERMEN KP) Nomor 16 Tahun 2022, didapatkan hasil bahwa 72% rajungan yang ditangkap, memenuhi ketentuan ditangkap dengan memenuhi ketentuan yang diperbolehkan dalam peraturan tersebut. Keywords: WPP 712, perairan utara jawa, gillnet. Share Link | Plain Format | Corresponding Author (Muhammad Arif Rahman)

153	Sustainable Aquaculture and Fisheries	ABS-52
Preliminary Study of onboard handling of the Blue Swimming Crabs in Fisheries Fleet Zarochman (a*), Suparman Sasmita (a), Zainal Wassahua (a), S.S Sukoraharjo (a) (a)National Research and Innovation Agency, Republic of Indonesia, Jl. Raya Bogor, KM. 46, Cibinong, Nanggewer Mekar, Bogor, East Java, 16912, Indonesia *Email: zaro001[at]brin.go.id Abstract The testing program is the beginning of the assessment phase of the blue swimming crab (BSC) fishing fleet with on board handling. Fishing gear for blue swimming crabs uses bubu (Traps). The fleet operation has been a one-day trip, so the handling of BSC and steaming are done on land. The target fishing ground is in waters > 20 meters deep. Engineering activities through assessment and application with immediate steaming handling at pasteurization temperature and storage of crab in a cold system on board. Program is attempting to achieve the target of optimal operational effectiveness of BSC fleet by producing innovations: The fleet components that are feasible and suitable for deeper waters with a 3- to 10-day for one trip. Studies are carried out on the suitability of ship, the design of mapping the fishing operation area, the steaming work order, and BSC of the ship^s storage with a rack arrangement. Storage can ensure the maintenance of quality of BSC with a larger size, greater yield content of crab meat, increased catch capacity, and high economic value. Information system of mapped fishing grounds as a material for analyzing the formation of fishing grounds in WPPNRI 712. Keywords: fleets, blue swimming crabs, onboard handling, bubu Share Link | Plain Format | Corresponding Author (Zarochman Zarochman)

154	Sustainable Aquaculture and Fisheries	ABS-53
Nutrient Content of Various Water-Based Alternative Protein Sources as Candidates for Raw Material Feed in Aquaculture S Saputra1, S Antoro1, SB Dhoe1, MAL Rajamuddin2, M Setiawati3, MA Suprayudi3 1Research Center for Fishery, National Research and Innovation Agency, Indonesia 2Pangkep State Polytechnic of Agriculture, Indonesia 3Aquaculture Department. Fisheries and Marine Science, Bogor Agricultural University, Indonesia Abstract In aquaculture, fish meal is the primary feed ingredient. The high growth of the aquaculture industry causes the need for fish meal to increase, so that the price of fish meal increases which in turn the operational costs of aquaculture also increasing. The purpose of this research is to evaluate water-based alternative protein sources as candidates for raw materials feed in aquaculture. Microalga (Tetraselmis chuii, Spirulina platensis, and Chaetoceros calcitrans), biofloc, and klekap were evaluated using proximate analysis, amino acid analysis and fatty acid analysis. The proximate analysis revealed that the microalga T. chuii meal (TcM), S. platensis meal (SpM), and biofloc meal (BFM) had a nutrient protein content greater than 20%, with an amino acid content comparable to fish meal and soybean meal. BFM and C. calcitrans meal (CcM) contain essential fatty acids required by both freshwater (linoleic fatty acid) and marine fish (EPA and DHA). Meanwhile, TcM, which was the only raw material, contained linolenic acid but no DHA, whereas SpM and klekap meal (KM) contained only linoleic fatty acid. Keywords: nutrient, protein, biofloc, klekap, microalgae Share Link | Plain Format | Corresponding Author (Suryadi Saputra)

155	Sustainable Aquaculture and Fisheries	ABS-54
Analysis of the immune parameters of shrimp fed diets supplemented with Phaeodactylum tricornutum. Ince Ayu K.Kadriah*1 Muliani1, Nurbaya1,Kamaruddin1, Rafly Ian Saharani2, Patang2 and Andi Sukainah2 1 Fisheries Research Center. National Research and Innovation Agency of Republic Indonesia. Cibinong West Java. 2 Faculty of Engineering, Makassar State University Jl. Dg. Tata Raya Parangtambung Makassar Abstract Currently, one of the approaches that is widely used in efforts to prevent shrimp disease is to increase shrimp immunity. The use of several natural ingredients has been tried to improve the performance of shrimp immun parameters, including the use of mangrove leaf extract and microalgae extract. In this study, supernatant extract and ethyl acetate extract from the microalgae Phaeodactylum sp will be used as immunostimulator to improve the immune system function of shrimp. Microalgae supernatants and ethyl acetate extracts are mixed into the feed formula as much as 35% of the feed weight. The test animal used was a 35-day-old shrimp juvenile. Supplemented feeding is carried out for 30 days before the challenge test. During the 30-day maintenance process, haemolimph sampling was carried out every 5 days to determine the THC value and Pro-PO activity. After the infection is carried out, haemolimph sampling is carried out once every 3 days for 7 days of maintenance. The calculation of shrimp survival after infection is carried out daily. The results showed that the addition of Phaeodactylum sp supernatant extract to shrimp feed was able to stabilize and maintain shrimp immune parameters when pathogenic bacterial infection occurred. Keywords: Phaeodactylum tricornutum, shrimp disease, Vibriosis, Immune system Share Link | Plain Format | Corresponding Author (Ince Ayu Khairana Kadriah)

156	Sustainable Aquaculture and Fisheries	ABS-55
Fish Stock Assesment of Fish Resources Common Ponyfish (Leiognathus equulus) Caught Traditional Set Net Fishing gear in Awangpone Waters Bone Regency Fijwal Patangngari, Musbir Musbir, Siti Khadijah Srioktoviana Hasanuddin University, Makassar, Indonesia Faculty of Marine Science and Fisheries, Capture Fisheries Study Program Abstract Common Ponyfish is the dominant fish in the Awangpone Waters, exploited throughout the year so it is feared that its population will decline. This study aims to analyze aspects of populist dynamics including age group, population growth, mortality, exploitation rate, yield per recruitment. Data on the total length of fish is collected through measurement of catches from February, March 2023. Age groups were analyzed using the Battacharya method, L&#8734- and K allegedly using the Ford and Walford methods, the Pauly Empirical M method, Z, F and E the Beverton and Holt methods. Data processing with FISAT-II and Microsoft Excel software. The results of the study that common ponyfish have a total length range of 4 to17.6 cm, the size of the most catches is in the middle of the class of 10 to12 cm and an average length of 13.06 to 2.15 cm. The population consists of one age group, the presumptive values L: 20 cm, K:1 per year and t0: -0.17777 years. Conjectures Z, M and F amounted to 4.32 per year, 2.06 per year and 2.27 per year, respectively. The exploitation rate was 0.52 per year and the actual, optimal Y/R were 0.0205 gr/recruitment and 0.0203 gr/recruitment, respectively. Keywords: Common ponyfish, growth, mortality, exploitation, recruitment Share Link | Plain Format | Corresponding Author (Musbir Musbir)

157	Sustainable Aquaculture and Fisheries	ABS-61
TECHNICAL REVIEW OF ENCIRCLING RINGED GILL NET FOR GILLNET STANDARDIZATION IN INDONESIA Zarochman(a*), S.S Sukoraharjo(a), Zainal Wassahua(a), Suparman Sasmita(a) (a)National Research and Innovation Agency, Republic of Indonesia, Jl. Raya Bogor, KM. 46, Cibinong, Nanggewer Mekar, Bogor, East Java, 16912, Indonesia *Email: zaro001[at]brin.go.id Abstract In proposed for fishing gear standardization based on fishing method is carried out the application of the theoretical formula to calculate fishing gear design for preparing fishing gear standardization in Indonesia. The result of the identification of encircling ringed gillnet belonging the local fishermen associated with the size of black pomfret as fished target is used for preparation of fishing gear standardization materials for the concerned by adjusting the size of fish target by measuring a fish length of about 150-250 mm or a fish body circumference of about 300-400 mm. As a result, the encircling ringed gillnet is made of nylon monofilament diameter 0,35 mm, and PE 380 d/6 mesh size 76,2 mm, Mesh length (ML) 2.879, and Mesh depth (MD) = 218 mesh with a head rope length of 120,65 m shorter than the ground rope 142,6 m. This gill net has a buoyancy of 173,6 grf/m and a sinking power of 124,7 grf/m or a buoyancy ratio of 1.39 sinking power. The gill net is trapezium shaped, operated surrounding of the net to fish schooling closed around FAD nearby in the waters of more than 20 m deep with an attached net height of 13.30 m. Keywords: standardization, fishing gear, encircling ringed gillnet Share Link | Plain Format | Corresponding Author (Zarochman Zarochman)

158	Sustainable Aquaculture and Fisheries	ABS-62
EFFECT OF LIMB AUTOTOMY ON THE INTERNAL PHYSIOLOGY AND BIOCHEMISTRY PARAMETERS, AND THE MOULTING PROCESS OF THE ORANGE MUD CRAB, Scylla olivacea. Rusydi Rozaimi, Ghazali Azmie, Muhammad Achdiat, Hanafiah Fazhan, Khor Waiho Abstract Limb autotomy is one of the behavioural adaptations of mud crabs, which is to shed off their limbs when stressed or threatened by predators. The orange mud crab, Scylla olivacea, is the most abundant mud crab in Malaysia. They are one of the high valued seafood delicacies and have commercially important values in the fisheries and aquaculture sector. Moulting in mud crabs is directly related to growth and maturation, and is an important process in its culture and production. In this study, we evaluated the moulting process of S. olivacea at the intermoult stage following different degrees of limb autotomy. The physiology of the mud crab was affected by limb autotomy, such as its eating behaviour, growth rate, regeneration rate, and moulting. Limb autotomy affected feeding negatively but promoted growth and regeneration rate, especially for bilateral claw ablation followed by full limb autotomy. Also, limb autotomy will induce ecdysteroid production as ecdysteroid promotes the moulting process. Understanding how limb autotomy induces moulting will provide important basis for future development of artificial moult induction strategies. Keywords: Autotomy- Portunids- Orange mud crab- Scylla olivacea Share Link | Plain Format | Corresponding Author (Rusydi Rozaimi)

159	Sustainable Aquaculture and Fisheries	ABS-66
Length Structure and Gonad Maturity of coastal trevally (Carangoides coeruleopinnatus) caught around seaweed area Musbir Musbir 1), Najamuddin 2), Muhammad Kurnia 3). Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Abstract Marine coastal in southern part of Bulukumba South Sulawesi is one of important area for the seaweeds culture and fishing activity especially small scale fishery. One of the fishes species caught in this area was a coastal trevally, Carangoides coeruleopinnatus. This study describes for the length structure and gonad maturity of C. coeruleopinnatus. caught from mcoastal in southern part of Bulukumba South Sulawesi. Monthly sample collection from March 2022 to Pebruary 2023 was done. A total of 376 individuals comprise of 195 males (51.86 %) ranged from 6.1 cm to 26.3 cm and 181 females (48.14%) ranged from 7.2 cm to 26.1 cm were observed. the sample population of sex ratio was not significantly different. In this study, stage III and stages IV were considered mature. The standard length at maturity ranged from 13.4 cm to 26.3 cm for males, and 11.2 cm-26.1 cm for females. The percentage of maturity fishes caught 28 % - 49 %. Keywords: coastal trevally, standard length, maturity fishes, seaweed area Share Link | Plain Format | Corresponding Author (Musbir Musbir)

160	Sustainable Aquaculture and Fisheries	ABS-67
SEBARAN PANJANG DAN KELAYAKAN TANGKAP IKAN BIJI NANGKA (Upeneus sulphureus) YANG TERTANGKAP DISEKITAR AREA RUMPUT LAUT Musbir Musbir 1), Najamuddin 2), Muhammad Kurnia Departemen Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin, Makassar, Indonesia Abstract Ikan biji Nangka (Upeneus sulphureus) adalah jenis ikan demersal dan merupakan salah satu ikan yang sering tertangka disekitar area rumput laut. Penelitian mengenai ikan biji nangka yang tertangkap disekitar area rumput laut belum banyak dilakukan. Penelitian ini bertujuan untuk mengetahui sebaran ukuran dan persentasi layak tangkap ikan biji nangka yang tertangkap disekitar area rumput laut. Penangkapan ikan dilakukan dengan mengunakan jaring insang dasar. Hasil penelitian menunjukkan bahwa ukuran ikan yang tertangkap berada pada kisaran 70-210 mm dengan hasil tangkapan terbanyak pada kisaran panjang 10-11 cm. Jumlah ikan yang tidak layak tangkap tetapi tertangkap adalah 45 %. Goatfish (Upeneus sulphureus) is a demersal fish and one of the caught fishes ikn area seaweed area. The study of goatfish caught in around seaweed area is very limit. More over the purpose of this study was to analysis the distribution of total length and percentage allowable catch of goat fish caught in around sea weed area. The fishes caught by using bottom gillnet. The result of study show that the distribution of fish total length ranged 70-210 mm with highest catch ranged 100-110 mm. The percentage of unfeasible catch of fish caught was 45 %. Keywords: goat fish, total length, allowable catch, seaweeds area Share Link | Plain Format | Corresponding Author (Musbir Musbir)

161	Sustainable Aquaculture and Fisheries	ABS-71
Evaluation of Integrated Periphyton System (IPS) aquaculture of Mixed-sex Giant Freshwater Prawn (Macrobrachium rosenbergii) David Marioni1*, Nor Azman Kasan1, Mhd Ikhwanuddin1,* 1 Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Malaysia Abstract An Aquamat-like media in an Integrated Periphyton System (IPS) was applied to mixed-sex Giant Freshwater Prawn (Macrobrachium rosenbergii) culture with outputs compared to Recirculating Aquaculture System (RAS) culture, stocked in triplicate at initial density of 1,000 m-2, and where carbon source was used to support heterotrophic biomass. In nursery (up to 32 days) the body weight and survival rate were similar. Growth performance was also similar when a 60 % subset of the produced nursery prawns of approximately less than 1 g body weight were stocked to a second post-nursery stage utilizing the two systems, identically configured to the prior stage. A second 40 % subset of the nursery prawns of approximately greater than 1 g body weight was stocked to a semi-commercial IPS tank. The two post-nursery runs (from 33 to 90 days) were harvested with gender identification, and performance was compared across gender groupings and male morphotypes at harvest. Evaluation of the statistical events supported observations regarding differential in performance from nursery to post-nursery for M. rosenbergii. The technique of size separation of nursery stock ahead of grow out was evaluated. Evidence from prior experimentation is presented in discussion of water quality analysis, the application of inferential statistics to the program, and the validity of outputs. Keywords: Evaluation of Integrated Periphyton System (IPS) aquaculture of mixed-sex Giant Freshwater Prawn, Macrobrachium rosenbergii (De Man, 1879) Share Link | Plain Format | Corresponding Author (DAVID MARIONI DAVID MARIONI)

162	Sustainable Aquaculture and Fisheries	ABS-73
The utilization of kopasanda (Chromolaena odorata) leaf flour to inhibit bacterial population and increase the growth of tiger shrimp Penaeus monodon in the pond Harlina Harlina, Rosmiati Rosmiati, Andi Hamdillah Universitas Muslim Indonesia Abstract One of the bacterial diseases faced by the cultivation of tiger shrimp is vibriosis. This study investigated the efficiency of using Chromolaena odorata leaf flour as a natural antibacterial agent and promoter for tiger shrimp Penaeus monodon growth. Shrimp was given artificial feed for 120 days with one of two feeds- diet without kopasanda leaf flour (control) or diet with leaf flour kopasanda. After 30 days of maintenance, there was an increase in vibrio population (105 CFU/ mL) on maintenance water in control ponds. By comparison, the number of Vibrio sp. in the experimental pond water was relatively constant at 102 CFU/ mL, and there were no drastic changes until the end of maintenance. Artificially fed shrimp enriched with kopasanda leaf flour showed a 1.08 times higher increase in specific growth rate (SGR) than the control group after 30 days of maintenance. After 120 days of maintenance, fed shrimp enriched with kopasanda leaf flour also showed an average specific growth rate of 5.92% with an average growth of 0.20 g day-1. The utilization of feed enriched with kopasanda leaf flour provides benefits to reduce Vibrio sp. and improve shrimp growth performance. Keywords: Feed conversion ratio- Medicinal plant- Vibriosis- Vibrio sp- Water quality Share Link | Plain Format | Corresponding Author (Harlina Harlina)

163	Sustainable Aquaculture and Fisheries	ABS-79
Biologically Sustainable Fishery Management of Pahang State Commercial Fishery in East Coast of Peninsular Malaysia: Surplus Production Modelling Approach Moe Shwe Sin, Lavaniya Gopabala Krishanan, Tai Shzee Yew Faculty of Business, Economics and Social Development, Universiti Malaysia Terengganu Abstract The management of commercial fishing industry in Malaysia need more efficient policies towards biologically and economically sustainable fishery. Overcapacity and overexploitation in the fishery would degrade and perhaps deplete fisheries populations in the case of open access. The Malaysian east coast economic region (ECER) includes the states of Kelantan, Terengganu, Pahang, and the Johor district of Mersing, and, in Pahang area, the number of licenses fishing vessels exceeds above the quota since the late 1980s. In Pahang state, there are two different type of fishery: Traditional fishery and Commercial fishery. In commercial fishery, Trawler and Purse seine are two major fishing gear types and overcapacity is the major issue in these two fishing gears. The focus of the research includes to evaluate the exploitation status of the fisheries resources and to assess the biologically sustainable condition of the commercial fishery in Pahang State and to propose sustainable fishery management policies. The Gordon Schaefer surplus production modelling is used with the timeseries catch and effort data retrieved from Department of Fisheries Malaysia, Annual Fisheries Statistics data. The four models of surplus production functions are being used in the study: (1) Schaefer^s Model, (2) Fox Model, (3) Schnute Model and (4) The Clark, Yoshimoto, and Pooley (CY&P) model to determine the best-fit surplus production model for estimating the theoretical catch or landing. The results indicate that the exploitation status of the fisheries resources in Pahang state is over the biologically sustainable conditions of MSY (Maximum Sustainable Yield) and the fishing effort applied in the fishing industry is over the EMSY (Effort at MSY) level. The effort control policy and limiting overcapacity and overcapitalization of the commercial fishing industry in Pahang state is crucially needed to apply in the nearest future to sustain the fishery in biologically sustainable perception. Keywords: sustainable fishery management, commercial fishery, surplus production model, Malaysia Share Link | Plain Format | Corresponding Author (Moe Shwe Sin Sin)

164	Sustainable Aquaculture and Fisheries	ABS-80
Catch and Length-weight relationships of Silky shark (Carcharhinus falciformis) Prayudi Budi Utomo1, Arief Reza Fahlevi2, Dian Novianto3, Muhammad Taufik3, Umi Chodrijah3 1 Directorate General of Marine and Fisheries Product Competitivenes, Ministry of Marine Affairs and Fisheries 2 Coastal and Marine Resource Management Unit of Sorong, Directorate General of Management of Marine Spatial Planning, Ministry of Marine Affairs and Fisheries 3 Research Center for Fishery, National Research and Innovation Agency E-mail: dian.novianto[at]brin.go.id Abstract Shark fishing is a kind of fishery that is quite beneficial on Biak Island. The significant market demand for shark fishery goods implies that fishermen are still actively catching sharks as fishing targets. We present length-weight (LWR) relationships of the silky shark (n=128) caught by the commercial drift longline in the Biak Island part of South West Pacific between February to September 2021 and describe the supply chains of sharks in this fisheries. The type of longline employed is a surface longline with bullet tuna, skipjack tuna, yellowfin tuna and shark meat as bait with a maximum catch rate of 39 individuals and an average catch of 13 per setting, dominated by silky sharks. The total length ranged of silky shark between 140 - 234 cm for males and 173-225 cm for females, were predominantly caught at lengths between 196 and 210 cm TL. Furthermore, the relationships of length-weight relationships were significant with the coefficient of determination (r2) of around 0.74 with allometric positive growth patterns. A system of predetermined quotas governs the marketing of shark fisheries products. While shark meat is normally processed into salted fish that is traded in the Biak island and some of which is sold out from island, shark fins are typically dried and then exported. Keywords : Silky shark, length-weight relationships, drift longline Keywords: Silky shark, length-weight relationships, drift longline Share Link | Plain Format | Corresponding Author (Prayudi Budi Utomo)

165	Sustainable Aquaculture and Fisheries	ABS-82
Ecosystem Approach to Fisheries Management to Assess the Fisheries Management State of Singkarak Lake Resources, Indonesia Husnah Husnah (a*) husnahsamhudi@gmail.com, husn008@brin.go.id., Renny Puspasari, Setiya Triharyuni, Arianto(a), Syahroma Husni Nasution, Muhammad Suhaemi Syawal (b), Hafrizal Syandri (c), Sonny Koeshendrajana^ (d), Yosmaniar (e), Yoga Candra Ditya (f) a) Research Center for Fisheries, National Research, and Innovation Agency, Indonesia b) Research Center for Limnology and Water Resource, National Research and Innovation Agency, Indonesia c) Faculty of Fisheries, University of Bung Hatta, Indonesia d) Research Center for Secular and Economy Behavior, National Research and Innovation Agency, Indonesia e) Research Center for Conservation of Marine, Inland, and Small Island Conservation , National Research and Innovation Indonesia f) Research Center for Biotechnology, National Research, and Innovation Indonesia Abstract Lake Singkarak has been designated as one of Indonesia^s 15 critical lakes. At least 3.9 million people use the aquatic resources of Lake Singkarak as a source of life and livelihood. Fisheries is one of the sectors contribute to the food security, especially protein. Various anthropogenic activities degraded the ecosystem and decreased fisheries production and biodiversity. There is no information on the status of management, especially fisheries resources. The current management status is an initial description of potential and existing problems that will be used to develop management plans and strategies for the future. Ecosystem Approach for Fisheries Management (EAFM) is one approach to determining the management status of a resource-based that has been adopted in Indonesia but is still limited to marine fisheries resources. As for inland fishery resources, it is still limited. The study was to assess the status of fisheries resource management in Lake Singkarak. The study was conducted using a rapid approach through structured interviews with decision makers and beneficiaries in Tanah Datar and Solok Regencies. The management component is divided into the main domains, and indicators. Indicator assessment is carried out by multicriteria analysis. The results showed that fisheries resource management in both districts was classified as being in implementing EAFM. Fishing technology, social and governance are three issues in Solok Regency, while in Tanah Datar Regency, the main issues are the environment of fish resources, economy, and groups of fish species managed. Keywords: Fisheries management, tectonic lake, priority lake, endemic fish, anthropogenic, multicriteria lease Just Try to Submit This Sample Abstract Share Link | Plain Format | Corresponding Author (Husnah Husnah)

166	Sustainable Aquaculture and Fisheries	ABS-88
Prevention of White Spot Syndrome Virus in tiger shrimp fingerling by using Sonneratia alba leaf boiling extract Nurhidayah, Muliani, Endang Susianingsih, Ince Ayu Khairana Kadriah and Muharijadi Atmomarsono National Research and Innovation Agency Abstract This study aimed to determine the effect of different immersions in a 1% solution of Sonneratia alba extract on the survival rate of fingerling tiger shrimp after being infected with WSSV. The study was carried out in a completely randomized design with eight treatments, namely A) 12-h immersion- B) 24- C) 36- D) 48- E) 60- F) 72- G) Positive control (infected WSSV but without mangrove extract)- and H) Negative control (without WSSV infection and mangrove extract), with three replications. The stocking density of fingerling was 20 individual/jar. All immersion methods (treatments A to F) were carried out before WSSV infection (concentration 0.1%). During the experiment, fingerling shrimp were fed three times a day as much as 20% of the total biomass per day. Mortality of fingerling was observed every day until eight days. Water quality parameters, including TOM, NH3-N, and NO2-N, were measured at the study^s beginning and end. The results showed that the highest survival rate (85.0%) was obtained by treatment C, while the lowest survival rate (43,33%) was achieved by positive control (treatment G). Soaking the extract of the boiled leaves of Soneratia alba mangrove can increase the resistance of fingerling tiger shrimp from WSSV attack most effectively for 36 hours Keywords: Sonneratia alba, mangrove extract, immersion, WSSV prevention, fingerling tiger shrimp Share Link | Plain Format | Corresponding Author (Nurhidayah Nurhidayah)

167	Sustainable Aquaculture and Fisheries	ABS-89
PERFORMANCE OF GENERATION 2 (G2) MILKFISH (Chanos chanos FORSKAL) SEED NURSERY IN DIFFERENT SALINITIES IN THE REARING CONTROLLED Tony Setia Dharma1, I Wayan Gede Astawa Karang2, Sri Harijati3 Gunawan1, Ananto Setiadi1 1Research Center and Fisheries Research Organizations for Earth Sciences and Maritime National Research and Innovation Agency 2Udayana University Denpasar Bali 3Bogor Institute Agriculture Bogor Abstract ABSTRACT This research aimed to evaluate the effect of salinity on survival and growth of milkfish fry as well as to determine optimum salinity to obtain the highest survival and body energy level. It was research with 5 different salinity treatments (13, 18, 23, 28 and 33 ppt) and 3 replications. Data analyses with ANOVA with level of significance 0.05.The results showed that salinity affected immensely on the survival and growth of milkfish fry (p<0,01). The highest survival was in those treatments 13, 18 and 23 ppt with survival rate 75.87 %, 73.67 % and 70.33 %, respectively. While survival rate of treatments 28 and 33 ppt were 65.87 % and 59.40 %, respectively. Salinity also affected the growth of milkfish fry (p<0.01) both on body length and weight. The highest body length was in the treatment 13 ppt, followed by in those treatments 18, 23, 28 and 33 ppt with 8.75, 7.98, 7.91, 7.77 and 7.06, respectively. The highest body weight was in the treatment 13 ppt, followed by in those treatments 18, 23, 28 and 33 ppt with 8.21, 7.50, 6.62, 6.28 and 6.39 g, respectively. There was also a significant difference (p<0.01) on the body^s energy level of milkfish fry from those treatments. The lowest body energy level was in the treatment 13 ppt followed by in those treatments 18, 23, 28, and 33 ppt with 4075.32, 4260.49, 4475.28, 4510.10, and 4695.21 kcal/g, respectively. It be concluded that optimum salinity for milkfish fry nursery was 13-23 ppt. Keywords: salinity, nursery, generation 2, and milkfish fry Share Link | Plain Format | Corresponding Author (Tony Setia Dharma Dharma)

168	Sustainable Aquaculture and Fisheries	ABS-91
Comparative Sudy on the Use of Two Commercial Diets for Grow-out of Golden Rabbitfish, Siganus guttatus Reared in Sea Cages Agus Nawang(a*), Ramadhan (b), Rosni (c), SRH Mulyaningrum(a), Lideman(a), MC Undu(d), Muhamad Safri(a), Syaiful Ardyansya(a), Tuti Asriani(c), Asda Laining(a) a. Research Center for Fishery, National Research and Innovation Agency, Cibinong, Bogor, Indonesia b Research Institute for Coastal Aquaculture and Fisheries Extension (RICAFE), Ministry of Marine Affairs and Fisheries, Maros 90512, South Sulawesi, Indonesia c. Research Center for Conservation of Marine and Inland Water Resources, National Research and Innovation Agency, Cibinong, Bogor, Indonesia d. Polytechnic of Marine and Fisheries of Jembrana, Ministry of Marine Affairs and Fisheries, Jembrana, Bali, Indonesia Abstract A 2x2x2 Factorial design was arranged to evaluate the effects of two factors which were commercial diet containing different protein levels of 22 and 26%, respectively and weight of golden rabbitfish (small and big) of S.guttatus on growth, feed utilization and proximate content of fillet when reared in floating sea cages. The feeding trial used 8 cages of 2x2x2 m3 stocked with 50 fishes per cage. All cages were fed twice a day to satiation. Monitoring growth was conducted by monthly sampling. After 90 days, survival rate ranged from 90.8 to 97% and no significant differences among all groups. The growth response indicated by weight gain (WG) and specific growth rate (SGR) demonstrated similar trend that only fish weight significanty affected the growth and no interaction effect between type of commercial diet and weight of fish were detected on this parameter. Feed intake was not affected either types of commercial diet or weight of rabbitfish. In contrast, feed concersion rasio (FRC) was significantly influenced by weight of fish, but not affected by types of feed used. Moreover, interaction between two factors was sifnificantly detected on FCR. Lipid content in fillet was significantly affected by the type of diet used and higher in the big fish when fed with diet contain 26% protein level. In conclusion, small fish grew faster than big fish and types of diet had similar effect on two size weights of fish groups. Small fish had better FCR when fed with diet containing 26% crude protein. Keywords: Commercial diet- fish size- grow-out- golden rabbitfish Share Link | Plain Format | Corresponding Author (Agus Nawang)

169	Sustainable Aquaculture and Fisheries	ABS-93
Gonad maturation and spawning of Gamat sea cucumber, Stichopus sp. reared in captivity Ketut Maha Setiawati1), Jhon Harianto Hutapea1), Zeny Widiastuti2), Made Buda1) and I Nyoman Adiasmara Giri1) 1) Research Center for Fisheries, Indonesia National Research and Innovation Agency 2) Brackishwater Aquaculture Development Center-Situbondo Abstract The exploitation rate of sea cucumber (Stichopus sp) has reached over exploitation, resulting in natural population decline. Therefore, a cultivation effort is needed to maintain the Gamat processing business and also as an effort to reduce the exploitation of the natural population. Understanding the spawning profile of sea cucumber in captivity was the main objective in this study. Observation include broodstock growth, spawning pattern, number of eggs produced, eggs diameter and hatching rate. Candidate broodstock is obtained from the Northern Bali Sea and then reared in a fiber glass tank with 4 qubic meters. During rearing, water exchange rate was 400 % per day candidate broodstock were fed with Sargassum sp and Ulva sp at a dose of 2.5 % of the body weight per day. Spawning is carried out from 2020 to 2022. The number of broodstock spawned were 20 individual per month with an average body weight size 0f 260 +/- 90 g. The results showed that sea cucumber broodstock can spawned throughout the year with the number of eggs varying from 105,000-3,000,000 eggs, egg diameter 180-270 micro meter and hatching rate ranging from 38.0-99.5 %. Spawning carried out every new moon for 3consecutive years shows a tendency to decline the frequency of spawning. Keywords: Eggs diameter, hatching rate, number of eggs, spawning profile, Sticophus sp Share Link | Plain Format | Corresponding Author (Ir. M.Sc.)

170	Sustainable Aquaculture and Fisheries	ABS-100
GROWTH AND QUALITY OF SEAWEED CULTURED IN WASTEWATER OF INTENSIVE SHRIMP PONDS 1Muhammad Syahrir, 2Radjuddin Syamsuddin, 2Haryati Tandipayuk, 2Dody Dharmawan Trijuno 1Doctoral Program in Fisheries Science, Graduate School of Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar, South Sulawesi 90245, Indonnesia/ Departemen of Aquacultur Bone Marine and Fisheries Polytecnic-2Department of Aquaculture, Faculty of Fisheries and Marine Science, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar, South Sulawesi 90245, Indonnesia- Abstract Seaweed is the one of the prima donna commodities in the fishery sectore because it has a high economic price and a good export market share. Seaweed can be used for various raw materials in an effort to meet human needs, such as- food needs, medicines and cosmetics. The purpose of the study was to analyze the marked differences between 3 types of grass that have the potential to be cultivated against the use of intensive shrimp pond wastewateras a cultivation medium. The research is carried out on laboratory scale with the method of quantitative research approach with experimental types of research. The ingredients used in this study were 3 types of seaweed (K.alvarezii, G.verrucosa, and E.spinosum). Measured variables include- growth by calculating the absolute weight (final weight-initial weight), carrageenan, chlorophyll, and carotenoid contenst. The design carried out was a complete randomized design (RAL) with 3 repeats with 2 measurement times, namely, the initial measurement (T0) and the final measurement (T1). The data analysis method used is parametric statistical analysis with the Anova (Analysis of variance) method. The result of the analysis obtained that the maintenance (cultivation) of seaweed in water waste media trough intensive shrimp ponds can be used to increase seaweed growth, chlorophyll, and carotenoid content. Keywords: Growth, carrageenan, chlorophyll, carotenoid, ANOVA Share Link | Plain Format | Corresponding Author (Muhammad Syahrir)

171	Sustainable Aquaculture and Fisheries	ABS-101
Endoparasite Infestation of Red Hybrid Tilapia (Oreochromis spp.) in Relation to Water Quality and Fish Farm Types Nur Asha Shahira Zamri (1), Sharifah Raina Manaf (1), Salwati Baharudin (1), Arif Azizi Che Harun (1), Mohd Ihwan Zakariah (2), Gunarto Latama (3) 1 Fakulti Perladangan dan Agroteknologi, Universiti Teknologi Mara UiTM Cawangan Sarawak Kampus Mukah. 2 Higher Institution Centre of Excellence, Institute of Tropical and Aquaculture and Fisheries (AKUATROP), University Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, Malaysia. 3 Universitas Hasanuddin, Makassar, Indonesia Abstract Water quality is one of crucial things in aquaculture. Water quality has varied effects to animals health. One of diseases events that can occur in aquaculture farms is endoparasite infestation. The study investigated the endoparasite affecting farmed red hybrid tilapia, Oreochromis spp. in 4 different locations in Sarawak, Malaysia. Hundred twenty farmed red hybrid Tilapia were sampled out from this study throughout June to December 2022 from four different district (Kuching, Miri, Mukah and Sibu). This study included different types of fish farms, earthen pond, concrete pond, and fish cage. Water quality parameters for four ponds were evaluated in situ using multiprobe and ex situ, in the laboratory. The result shows high concentration of nitrite nitrate, phosphate and ammonium recorded from concrete pond. However out of four farms, the study revealed high number of digenean trematode endoparasite found mostly in stomach and intestine of farmed red hybrid tilapia reared in earthen pond. The finding shows different result for endoparasite infestation in relation to water quality. Though certain condition of water quality parameters could be optimum environment for endoparasite life cycle, controllable environment in fish farm should be utilized to ensure health and the profitability of fish farming. Keywords: Endoparasite, Tilapia, Fish Farm, Water Quality Share Link | Plain Format | Corresponding Author (Nur Asha Shahira Binti Zamri)

172	Sustainable Aquaculture and Fisheries	ABS-104
Legal Protection for the Environment and Coastal Communities Against the Threat of Marine Ecosystem Degradation Naswar, Aminuddin Ilmar, Achmad, Maskun, Amaliyah, M. Zakir, N H AlMukaramah Faculty of Law, Hasanuddin University Abstract Climate change is impacting the global marine environment at unprecedented levels, causing multifaceted implications for marine and coastal ecosystems. This study aims to examine legal instruments, within the framework of Indonesia^s positive law, and provide legal protection for the environment and coastal communities who are facing threats of degradation of marine ecosystems. This study applies the normative-empirical legal research method, which focuses on the study of the harmonization of regulations and policies related to environmental protection and coastal communities. Normative research generally uses several approaches, including conceptual approaches, historical approaches, statutory (conventional) approaches, comparative approaches, and case approaches. This research shows that legal protection for the environment and coastal communities is carried out by applying legal instruments to regulate communities and other stakeholders in preventing the degradation of marine ecosystems. Keywords: Environment- Coastal Society- Marine Ecosystem Degradation Share Link | Plain Format | Corresponding Author (Maskun Maskun)

173	Sustainable Aquaculture and Fisheries	ABS-106
Measuring the fish logistics performance index in the Indonesian fisheries supply chains Prayudi Budi Utomo1, Berny A. Subki1, Harlin1, Usep Suhendar1, Indah Kusharyanti1, Ulfa Khaerawati Hamdan1, Eviet Sri Setiyorini1, Tri Hardi Putra1, Tri Hardi Putra1, Muhammad Hanief Ihsanuddin1, Dian Novianto2, Taryono3 1Directorate General of Marine and Fisheries Product Competitivenes, Ministry of Marine Affairs and Fisheries, Jakarta, Indonesia 2Research Center for Fishery, National Research and Innovation Agency, Cibinong, Indonesia 3IPB University Email : prayudi[at]kkp.go.id Abstract Indonesia is an archipelagic nation and is observed experiencing an unequal distribution in terms of population, economic access and goods distribution including fisheries products between western and eastern part of the country. To tackle such challenges The Indonesian Ministry of Marine Affairs and Fisheries launched the National Fish Logistics System (SLIN) since 2014 which has objective to develop an integrated, effective, and efficient fish and fishery product supply chain management. Some government efforts have been taken in place to achieve SLIN goals by providing cool chain facilities, capacity building of supply chain actors, empowering institution through coordinating private and government and others. Six corridors of implementation along Indonesia fish supply chain from production to distribution centre have been established as priority location. In this study, we analysed a logistics performance approach by measuring the fish logistics performance index (IKLI) in the corridor of Mimika - Surabaya/Jakarta in 2021. The study calculation resulted in an IKLI score of 65.74 which is included in the ^Good^ category. The dimensions of ^benefits^ and ^governance^ have the highest dimension index values of 92.32 and 81.91 (excellent) respectively, while the index value for product procurement management has the lowest dimension index value of 40.15 (poor) and for dimension of ^Efficiency^ and ^Connectivity^ are 53.03 (fair) and 71.59 (good). Keywords: National Fish Logistics System (SLIN), Logistic performance index, fish supply chain Share Link | Plain Format | Corresponding Author (Prayudi Budi Utomo)

174	Sustainable Aquaculture and Fisheries	ABS-109
Analisis Teknis dan Efektivitas Underwater LED Sebagai Alat Pengumpul Ikan pada BaganTancap Berbasis di Pesisir Pantai Pangkaje^ne, Kabupaten Pangkep Ummi Fatma, Muhammad Kurnia, Musbir, M. Abdul Ibnu Hajar, Fahrul Universitas Hasanuddin Abstract Penelitian ini bertujuan untuk mengetahui aspek teknis berupa ketahanan pencahayaan lampu bawah air dan efektivitas lampu celup underwater light emitting diode (LED) serta komposisi jenis dan jumlah hasil Tangkapan bagan tancap menggunakan underwater LED. Penelitian dilaksanakan pada bulan Juni - Agustus 2022 dengan mengikuti satu unit bagan tancap sebanyak 60 hauling. Hasil menunjukkan Underwater LED mampu menarik respon ikan dari berbagai jenis dan ukuran tetapi kurang dalam jumlah (kg). Lampu LED dengan intensitas cahaya 60 watt dalam penggunaanya kurang efektif tetapi ketahanan pencahayaan lampu dalam proses penelitian cukup baik. Komposisi jenis hasil tangkapan selama 60 hauling diperoleh sebanyak 15 spesies dengan jumlah hasil tangkapan sebanyak 106 kg. Kata kunci: Bagan tancap, Hasil tangkapan, Teknis dan Efektivitas LED Keywords: Bagan Tancap, Hasil tangkapan, Teknis dan Efektivitas LED Share Link | Plain Format | Corresponding Author (Ummi Fatma)

175	Sustainable Aquaculture and Fisheries	ABS-110
Comparative study of male and female shark catfish, Pangasius nasutus: analysis of biological indices and nutrients profiles as reproductive indicators Donald Torsabo1,2, Gabriel Arome Ataguba2, Benedict Terkula Iber1,2, Nurizzati Idris1 ,*Noordiyana Mat Noordin1,3, Ivan Chong Chu Koh3, Muhammad Yazed Abduh3, Ambok Bolong Abol-Munafi1 1. Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia 2. Department of Fisheries and Aquaculture, Federal University of Agriculture Makurdi, Makurdi, Benue State, Nigeria 3. Faculty of Fisheries and Food Science Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia Abstract Keywords: Shark catfish- age- gonad development- captive stock- Pangasius nasutus- fatty acid- otoliths Share Link | Plain Format | Corresponding Author (Donald Torsabo)

176	Sustainable Aquaculture and Fisheries	ABS-114
The Effect of Fermented Herbal Extracts on the Histological Appearance of the Intestine and Hepatopancreas in relation to the health of Vannamei Shrimp (Litopenaeus vannamei) Yushinta Fujaya, Dwi Kesuma Sari, Hasri, Andi Fahrul Syahrullah, Muhammad Rusdy Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Tamalanrea, Makassar, South Sulawesi Indonesia. Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar, Indonesia. Faculty of Medicine and Health Sciences, Alauddin State Islamic University, Makassar, 90245, Indonesia Abstract This study aims to compare the histological appearance of the intestine and hepatopancreas of vannamei shrimp given Fermented Herbal Extract (FHE) and without FHE. This research was conducted from December 2021 to April 2022. Vannamei shrimp by administering FHE was carried out in Tarusang Village and those without Vitomolt plus was carried out by cultivating communities in Japing-Japing Village, Labakkang District, Pangkep Regency, each with a maintenance period of 87 days . Preparation and observation of histological preparations were carried out at the Educational Verterinary Pathology Laboratory at Hasanuddin University using the standard Hematoxylin-Eosin method. The results showed that the addition of FHE to the feed had an effect on the histological appearance of the vannamei shrimp intestine and hepatopancreas compared to the control treatment. Supplementation of FHE showed in increasing the length of the villi, the distance between the villi, the number of villi, the number of goblet cells, and the number of lymphocyte cells. The hepatopancreas appears normal with a star-shaped lumen and intact tubules. This histological appearance explains why shrimp that received EHF supplementation were healthier and grew larger than those without EHF Keywords: Herbal Extract, Histology, Intestine, Hepatopancreas, Vannamei Share Link | Plain Format | Corresponding Author (Yushinta Fujaya)

177	Sustainable Aquaculture and Fisheries	ABS-116
Moulting stages in orange mud crab Scylla olivacea Ghazali, A., Fazhan, H.,, Ikhwanuddin, M., Achdiat, M., Rusydi, R., and Waiho, K. Higher Institution Center of Excellent (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu. Abstract Aquaculture is one of the fastest growing food production sectors in the world and plays a major role in global food security, including Malaysia. Mud crabs genus Scylla are among the commercially important seafood product in Malaysia with increasing demand from both domestic and international markets. However, among the major problems in juvenile crab culture and the production of soft-shell crab are the unsynchronised moulting that would lead to high cannibalism rate. Information on the detailed characterisation of the molting proces is limited and not elaborated in details in mud crab genus Scylla. Determination of the moulting stage in mud crab is important in the effort to synchronise the moulting process. Therefore, the present study was designed to identify and characterise in details the moulting stages in mud crab genus Scylla, via setal morphology and the epidermal retraction. Based on both characters, four moulting stages in orange mud crab, S. olivacea were determined and classified as Intermoult, Premoult (early and late premoult), Ecdysis and Postmoult. This study will be the first to provide a simple and practical method in determining the moulting stage (especially the premoult stage), where it is hope to serve as an essential baseline information in improving the aquaculture and fisheries management in general. Keywords: Mud crab, Scylla olivacea, moulting stages, softshell. Share Link | Plain Format | Corresponding Author (Azmie Ghazali)

178	Sustainable Aquaculture and Fisheries	ABS-117
ECO-FRIENDLY ANALYSIS OF GILL NET AND TRAMMEL NET BASED ON CODE OF CONDUCT FOR RESPONSIBLE FISHERIES (CCRF) IN BANGKALAN WATERS MADURA, EAST JAVA Gatut Bintoro*, Fuad, DGR Wiadnya, Mihrobi Khalwatu Rihmi, Tri Djoko Lelono, Syifa Jihan Raimahua Fisheries and Marine Science Faculty Universitas Brawijaya Abstract Java Sea or Fisheries Management Area (FMA) 712 directly adjacent to Bangkalan Regency has been overexploited. Arosbaya Sub-district in Bangkalan Regency makes the capture fisheries become the main income commodities. In Arosbaya gill net and trammel net commonly used by the fishermen. Both gill net and trammel net in Arosbaya have been optimized, but have not been tested for environmental friendliness. To optimize sustainable fisheries, Code of Conduct for Responsible Fisheries (CCRF) was initiated by the Food Agriculture Organization (FAO). CCRF became a global guideline for the implementation of responsible world fisheries. From this, the Ministry of Maritime Affairs and Fisheries of the Republic of Indonesia adapted and produced 9 criteria for environmental friendliness to determine the status of a fishing gear. The purpose of this research is to analyze and compare the level of environmental friendliness of gill net and trammel net in Arosbaya Waters based on CCRF criteria. Data collected in Arosbaya Waters, Bangkalan and used 100 respondents who were trammel net and gill net fishermen. Data obtained from observation, questionnaires and documentation. The data used is data that contains 9 criteria of eco-friendly fishing gear. Based on the results of the CCRF analysis, the trammel net got 28 points. Meanwhile, the gill net got 30 points. Thus, both are included in the category of extremely environmentally friendly fishing gear. Based on these results, gillnet is superior to trammel net in Arosbaya Waters Keywords: Environmental Friendliness, Fishing Gear, Java Sea, Responsible Management, Sustainable Capture fisheries Share Link | Plain Format | Corresponding Author (Gatut Bintoro)

179	Sustainable Aquaculture and Fisheries	ABS-120
Blue Crab (Callinectes sapidus) Spermathecae Performance in Three Tributaries Of The Upper Chesapeake Bay Yushinta Fujaya, Robert Aguilar, Roberta Bardelli, Kimberly D. Richie, Matthew B Ogburn Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar 90245, Indonesia- Smithsonian Environmental Research Center, Edgewater, MD 21037, USA Department of Earth and Marine Sciences, University of Palermo, Via Archirafi, 22, 90123, Palermo, Italy Abstract The blue crab, Callinectes sapidus, is a key species in the Chesapeake Bay that plays an integral ecological, economic, and sociological role. However, reports suggest that there has been a significant decline in the population of these crabs as well as the recruits per spawning female. The objective of this study was to analyze the current state of blue crab spermathecae performance in three tributaries of the upper Chesapeake Bay that vary in male crab exploitation rate. Mature blue crabs were collected from June to October in the Rhode, Choptank, and Patuxent rivers from fishery-independent trawls and commercial trotlines. Female crabs were brought to the Smithsonian Environmental Research Center (SERC) laboratory alive and male crabs were returned to the rivers after being recorded. The female crabs were stored in the freezer (-20oC) until further investigation. There were 180 female crabs used in this study. The parameters observed were the maturity stages of the ovary, spermathecae index, spermathecae fullness, and sperm count. The results indicate that there is variation by tributary. Keywords: Blue Crab, Spermathecae, Chesapeake Bay, Sperm count Share Link | Plain Format | Corresponding Author (Yushinta Fujaya)

180	Sustainable Aquaculture and Fisheries	ABS-123
Performance of Aquatic Plant Bacopa caroliniana as fish diseases prevention treatment for aquaculture in vitro test Media Fitri Isma Nugraha1, Mia Melvina2, Hessy Novita3, Muh Alias L. Rajamuddin4, Wening Enggarini5, Dwi Budiyanto Trisnoharjono6, Berna Elya2 1 Research Centre for Pharmaceutical Ingredients and Traditional Medicine-National Research and Innovation Agency of Indonesia 2 Faculty of Pharmacy, University of Indonesia, Depok 16424 West Java, Indonesia 3 Research Centre for Veterinary -National Research and Innovation Agency of Indonesia, Jakarta Indonesia 4 Pangkep State Polytechnic of Agriculture, South Sulawesi, 90655, Indonesia. 5 Research Center for Genetic Engineering - National Research and Innovation Agency 6 Research Center for Marine and Land Bioindustry- National Research and Innovation Agency of Indonesia Abstract Abstract: The genus Bacopa a type of aquatic plant that has been widely used in medicine Ayurvedic medicine. The performance of its bioactive compounds used as an antibacterial agent was observed to control fish diseases. Objective, This study was to reveal the content of antioxidant and antibacterial active compounds of Bacopa caroliniana and their effectiveness in controlling the growth of Aeromonas hydrophila, Streptococcus agalactiae, Flavobacterium columnare, Edwardsiella ictaluri, using in vitro methods. Materials and Methods, Bioactive compounds was extracted from Bacopacaroliniana by employing Ultrasound-Assisted Extraction. Extracts were screened qualitatively for antioxidant activity and then quantified to measure in vitro antioxidant activities using the 2.2 diphenyl 1 picrylhydrazyl DPPH radical scavenging assay and the ferric-reducing antioxidant power FRAP assay. The evaluation of chemical compounds was conducted using GCMS. Antibacterial activity was determined by the paper disc diffusion method and microdilution. Results, Bacopa caroliniana extracts were found to contain alkaloids, tannins, flavonoids, saponins, terpenoids, and glycosides. GCMS profiles showed that the extract had 13 identified compounds. Bacopa caroliniana herbal extract has strong antioxidant activity with IC50 value50 microgram per mL i.e. 40.37 microgram per mL. While, Bacopa caroliniana extract inhibited the growth of Aeromonas hydrophila, Streptococcus agalactiae, Flavobacterium columnare bacteria, but not for Edwardsiella ictaluri. Conclusion, The significant potential of secondary metabolites from Bacopa carolinianaindicates an opportunity as a new potential candidate for aquatic plants that have high antioxidant, antibacterial, and anti inflammatory activities. Keywords: Bacopa caroliniana, Aeromonas hydrophila, Streptococcus agalactiae, Flavobacterium columnare, Edwardsiella ictaluri. Share Link | Plain Format | Corresponding Author (Media Fitri Isma Nugraha)

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