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211	Sustainable Aquaculture and Fisheries	ABS-203
Determination of carrying capacity of waters for the development of intensive technology vaname shrimp ponds in Bulukumba Regency, South Sulawesi Province, Indonesiat Mudian Paena, Ruzkiah Asaf, Admi Athirah, Kamaria, Erna Ratnawati, Tarunamulia, Akhmad Mustafa dan Imam Tauhid National Research and Innovation Agency Abstract Intensive technology for vaname shrimp (Litopenaeus vannamei) ponds is an alternative technology to increase national shrimp production in addition to other technologies. Vaname shrimp production in ponds with intensive technology can generally reach 50 tons per hectare which is equivalent to shrimp production for 60 years using traditional technology. The application of intensive technology for sustainable vannamei shrimp pond cultivation can only be realized if expansion planning is based on environmental carrying capacity. Based on this, research has been carried out which aims to determine the carrying capacity of sea waters for the development of intensive vannamei shrimp ponds in Bulukumba Regency (Gantarang and Bontobahari Districts). Carrying capacity is calculated based on the content of oxygen (O) and nitrogen (N) in seawater. The results showed that in Gantarang District, intensive pond development could still be carried out based on the carrying capacity of oxygen and nitrogen, respectively 56.5 ha and 110 ha. Meanwhile, in Bontobahari District, intensive pond development can still be carried out based on the carrying capacity of oxygen and nitrogen of 65.5 ha and 118 ha, respectively. Keywords: Carrying capacity, shrimp farming, intensive technology Share Link | Plain Format | Corresponding Author (Mudian Paena)

212	Sustainable Aquaculture and Fisheries	ABS-205
Factors Affecting the Willingness to Adopt the Good Aquaculture Practices (GAPs) in Traditional Shrimp Farming in Pinrang Regency, Indonesia - Extension of the Theory of Planned Behavior within a Structural Equation Modeling Tarunamulia (a*), Erna Ratnawati (a), Kamariah (a), Ruzkiah Asaf (b), Admi Athirah (b) a) Research Centre for Fishery, National Research and Innovation Agency, Cibinong 16911, West Java, Indonesia *tarunamulia[at]brin.go.id b) Research Center for Conservation of Marine and Inland Water Resources, National Research and Innovation Agency, Cibinong 16911, West Java, Indonesia Abstract This study explores the factors influencing shrimp farmers^ adoption of Good Aquaculture Practices (GAPs) in traditionally managed aquaculture ponds cluster in Pinrang Regency, South Sulawesi Province, Indonesia. The factors influencing shrimp farmers^ decision to apply GAPs to traditional ponds in Pinrang Regency were analyzed using the Theory of Planned Behavior (TPB) with structural equation modeling (SEM). The analysis results showed that shrimp farmers^ willingness to adopt GAPs is strongly influenced by their attitude that GAPs can increase their production or income, improve a positive reputation in society and contribute to improving the aquaculture environment. Meanwhile, the main impetus for adopting GAPs came from exporters or local entrepreneurs and directives and assistance from the relevant government, universities, and academics. The shrimp farmers believe that to maximize the level of application and the adoption of GAPs, improving pond engineering and managing environmental limiting factors are of high priority. The decline in aquaculture pond water quality is closely related to the condition of ponds with problems exchanging water, particularly disposing of aquaculture waste after the operation. Keywords: shrimp culture, GAPs, adoption level Share Link | Plain Format | Corresponding Author (Tarunamulia Tarunamulia)

213	Sustainable Aquaculture and Fisheries	ABS-207
Application of Organic Fertilizer Originated from Shrimp Pond Solid Waste Using Different Bioactivators in Milkfish Nursery Chanos chanos (Forsskal, 1775) Hidayat Suryanto Suwoyo (a*) Sri Redjeki Hesti Mulyaningrum (a), Unggul Adi Utama (b), Subari Yanto (b), Amirah Mustarin (b), Makmur(a) and Andi Sahrijanna(a) (a) Research Center for Fishery, National Research and Innovation Agency (b) Agricultural Technology Education Study Program, Faculty of Engineering, Makassar State University Abstract Pond sediment was rich in total nitrogen, phosphor, potassium, organic matter, and other macro and micronutrients. Pond solid waste can be used as organic fertilizer through the composting process. The composting process could be run faster with the assistance of bioactivators. This study aimed to evaluate the effect of organic fertilizer application originating from shrimp pond solid waste using different bio activators on milkfish nursery, Chanos chanos (Forsskal, 1775). The study used a completely randomized design (CRD) with five treatments with 3 replications of respectively. The treatments combination of organic fertilizer composed of 2,000 kg/ha of pond solid waste+200 kg/ha of Urea + 100 kg/ha of SP-36 using five different bioactivators, namely: BIOTAN-28 (A), TR-04 (B), EM-4 (C), ISO (D), and organic fertilizer without bioactivator as control (E). Milkfish was reared in 30 days, observed variables were the composition and abundance of plankton, growth and survival rate of milkfish, and water quality. The results showed that the application of organic fertilizer originating from shrimp pond solid waste using different bio activators did not significantly different on the growth of natural feed, the growth and survival rate of milkfish (P>0.05) between treatment. The growth of milkfish obtained 0.35-0.78 g/ind with a survival rate ranging from 93.33-98.89%. The water quality obtained during the study still supports milkfish^s growth and survival rate. Keywords: bio activator, milkfish, nursery, pond shrimp solid waste, organic fertilizer Share Link | Plain Format | Corresponding Author (HIDAYAT SURYANTO SUWOYO)

214	Sustainable Aquaculture and Fisheries	ABS-210
Utilization of chicken feather meal for fish feed by hydrolysis and bacterial fermentation Desy Putri Handayani, Alim Isnansetyo Universitas Gadjah Mada Abstract Chicken feathers have enormous potential as a source of fish feed ingredients. The protein content of chicken feathers is very high, reaching 80-90% so that it can be used as an alternative to replace fish meal. This study aims to utilize chicken feathers as fish feed ingredients by hydrolysis and fermentation. The study used an experimental method with a completely randomized design (CRD) with 4 treatments, 3 replications, namely P0: chicken feather meal (control)- P1: chicken feather meal + 10% HCL- P2: chicken feather meal + 10% NaOH- P3: Fermentation with IW probiotics. Parameters observed were proximate test results including crude protein content, moisture content, ash content, crude fat content, crude fibre content, carbohydrates and supporting parameters (organoleptic test), in the form of physical properties of chicken feather flour, including odour, colour and texture. Data of proximate were analysed using ANOVA and Duncan Multiple Range Test (DMRT) with a test level of 5%, while for organoleptic results data were analysed descriptively and qualitatively. The results showed that the crude protein content of control, fermented with IW probiotic, hydrolysed using 10% HCL, and 10% NaOH, respectively were 63.94, 78.11, 71.86, and 56.28 %. Fermentation using IW probiotics can increase the crude protein content. The P3 treatment was the most effective treatment because it produced the highest protein, namely 78.11%, with a change in physical properties to a light brown colour, soft texture, and less typical sting. Keywords: chicken feather meal, crude protein, fermentation, fish feed ingredients, hydrolysis Share Link | Plain Format | Corresponding Author (Desy Putri Handayani)

215	Sustainable Aquaculture and Fisheries	ABS-212
The Inclusion Criteria Marine Tourism Based on Sustainability Fisheries Resources (Case Study in Nusa Penida, Indonesia) Atikah Nurhayati 1, Wahyuniar Pamungkas 2, Asep Agus Handaka 3, Nora Akbarsyah 4 Faculty of Fisheries and Marine Science, Universitas Padjadjaran Abstract The potential for developing marine tourism by optimizing fisheries resources to attract tourists requires sustainable governance. Marine tourism has particular potential for promoting inclusion and contributing to marine sustainable development. This research aims to analyses the inclusion criteria marine tourism based on sustainability fisheries resources (case study in Nusa Penida, Indonesia). The research method uses a quantitative description. Conducting research from June to October 2022. This research used primary and secondary data. The technique for taking respondents used purposive sampling, as many as 30 respondents. The analytical tool used is logistics regression. Based on the results of the study, it is necessary to manage multi-sectoral and multi-sectoral marine tourism areas involvement of the local community, developing gender equality in the management of marine tourism areas, providing education and concern from tourists towards conservation areas for fisheries and marine resources. Promoting inclusion in marine tourism is an effective way to maintain the balance of the ecosystem of fisheries resources with tourism activities, such as the attraction of manta pari diving tourism. Making sure women^s participation in these marine tourism works as well as possible is therefore very important for improving development outcomes in the coastal area. Keywords: coastal, fisheries, inclusion, tourism, gender. sustainability Share Link | Plain Format | Corresponding Author (Atikah Nurhayati)

216	Sustainable Aquaculture and Fisheries	ABS-216
Effect of microbubble aerator innovation on dissolved oxygen, carrying capacity and performance of red tilapia (Orechromis sp.) in the RAS system Rustadi (a*), Eny Heriyati (b), Alim Isnansetyo (a), Bambang Triyatmo (a), Indah Istiqomah (a), Deendarlianto (c) and Wiratni (d) (a)Department of Fisheries, Faculty of Agriculture, UGM, Yogyakarta Indonesia. (b)Marine Science Program study, Kutai Timur Agricultural College School Indonesia (c)Department of Mechanical Engineering, Faculty of Engineering, UGM, Yogyakarta, Indonesia. (d)Department of Chemical Engineering, Faculty of Engineering, UGM, Yogyakarta, Indonesia. Abstract This research was conducted to examine microbubble aerator compared to conventional aeration (blower) in formation of dissolved oxygen (DO), to measure standard oxygen transfer rate (SOTR) and standard aeration efficiency (SAE) as well as to consider the oxygen consumption of red tilapia in order to predict the optimum stocking density. The results showed that microbubble aerator produced DO 6.58 ppm higher (p<0.05) than that of blower with DO value of 5.59 ppm under air discharge of 3 L/min. The values of SOTR and SAE on microbubble aerator were 0.007 kg O2/h and 0.09 kg O2/KW/h, respectively, higher than those of SOTR and SAE on blower approximately 0.002 kgO2/h and 0.02 kgO2/KW/h respectively. This experiment also tested DO value on recirculation medium with air discharge of 0.03 L/sec, in which DO value of microbubble aerator was 7.44 ppm higher (p<0.05) than those of blower aerator of 6.51, and no aeration as control of 5.35 ppm. Microbubble aerator was capable of increasing 62% higher stocking density than blower aerator in recirculation system. It was concluded that microbubble aerator had higher DO value, transferred much oxygen and more saved the energy compared to blower aerator, and the prediction of stocking density using microbubble aerator was 62% higher than blower. Fish biomass was higher in microbubble aerator, with high survival and growth rate, and a lower FCR value, those indicating good culture performance. Keywords: DO, microbubble aerator, RAS, red tilapia, stocking density Share Link | Plain Format | Corresponding Author (Rustadi Rustadi)

217	Sustainable Aquaculture and Fisheries	ABS-217
Application of whole cell microalgae as an alternative to prevent vibriosis E. Susianingsih1, I.A.K. Kadriah1 Nurhidayah1 National Research and Innovation Agency Abstract Abstract.The more intensive aquaculture activities make the development of the disease increase. One of them is vibriosis, a disease caused by the pathogenic Vibrio bacteria. Several alternative disease prevention has been carried out. One of them is by using microalgae as biocontrol to control the growth of pathogenic bacteria. Naturally, bacteria and plankton (phytoplankton and zooplankton) interact with each other in aquatic ecosystems. In clean water conditions, good bacteria and plankton can grow in abundance. However, if the water quality getting worst, the pathogenic bacteria have the opportunity to grow better. This study aims to determine the effect of using whole cell microalgae on the growth of pathogenic bacteria. Using 5 types of microalgae namely: Melosira sp, Phaeodactylum sp, Nannochloropsis sp, Porphyridium aerugenum and Porphyridium S with a density of 105 cells/mL. Vibrio harveyi for challenge test was used with a density of 107 CFU/mL. In vitro challenge test was carried out using a completely randomized design with 6 treatments. Whereas in vivo it was carried out using PL 16 tiger shrimp at a density of 20 individuals/20 L volume. The results showed that in vitro administration of whole cell microalgae types P.aerugineum, Nannochloropsis sp and Melosira sp could inhibit the growth of pathogenic Vibrio bacteria, whereas in vivo administration of microalgae Porphyridium aerugineum and Phaeodactylum sp was able to inhibit the growth of pathogenic Vibrio bacteria better than other types of microalgae and control. Keywords: vibriosis, microalgae, challenge test, pathogenic vibrio, Share Link | Plain Format | Corresponding Author (Endang Susianingsih)

218	Sustainable Aquaculture and Fisheries	ABS-218
Identification of Pathogenic Bacteria on Giant Gourami (Osphronemus goramy Lac.) from Kulon Progo Regency of Yogyakarta Rigen Pawartika, Murwantoko Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada Abstract Giant gourami (Osphronemus goramy Lac.) is one of the important economic fish that has been widely cultured in Indonesia because it has many advantages. Bacteria are important pathogens especially in the tropics, given that bacteria can survive well in aquatic environments. The objective of this study is to identify the bacteria causing diseases on giant gourami from which cultured in the Regency of Kulon Progo, Yogyakarta. The diseased fishes were collected from the pond and observed for their external and internal clinical signs. Several organs were fixed and proceededfor histology. The bacteria were isolated from liver and kidney on the Tryticase Soy Agar medium, and followed by subculture to achieve pure culture bacteria. The pathogenic capability of bacteria isolates were proven by Koch^s postulate on juvenile gourami. The identification of the bacteria was conducted on the based of biochemical characteristic as well as 16S rRNA sequences. Isolate SW2H (Pc1)1.2 and SW2G.KC (Pc3)2 were confirmed as the bacterial pathogen in giant gourami as indicated by the similarity of the symptoms between natural infected and reinfected specimens, such as hemorrhage on the body surface, damaged fin, along with necrosis, congestion, and hemorrhage on the tissues. Biochemical characteristic Identification showed that SW2H (Pc1)1.2 and SW2G.KC (Pc3)2 isolates are Aeromonas veronii, which are Gram-negative, motile, catalase and oxidase bacteria. Analysis on 16S rRNA sequences supported that SW2H (Pc1)1.2 and SW2G.KC (Pc3)2 isolates are Aeromonas veronii with the identity with data in Genbank are 99.38% and 99.44% respectively. Keywords: Aeromonas veronii, hemorrhage, Koch^s postulate, 16S rRNA Share Link | Plain Format | Corresponding Author (Murwantoko Murwantoko)

219	Sustainable Aquaculture and Fisheries	ABS-219
Seaweeds and Blue economy in Indonesia: current state, opportunities, and challenges La Ode M. Aslan, Abd. Rahman Nurdin, Wa Ode Sifatu, L O Aslin Department of Aquaculture, Faculty of Fisheries and marine Science, Halu Oleo University Abstract Seaweed has been used widely in many sectors for food, cosmetics, nutraceuticals, pharmaceuticals and bioenergy. In these recent days, seaweed is a main element for blue growth and is expected to contribute significantly to the development of the growing Indonesian blue economy. Several R & D programs and Innovation projects in Indonesia result in the establishment and development of a seaweed farming in the most Indonesian coastal areas. However, seaweed cultivation and bioprocessing is still limited in the farming area, particularly in eastern Indonesia where seaweeds are abundant on its coast. Ongoing projects from different research institutes demonstrate that seaweeds from the Indonesian coasts are a source of valuable compounds and that they can be used as a natural renewable resource suitable for many industrial applications. Furthermore, specific research activities on seaweed cultivation have been conducted particularly for Kappaphycus and Gracillaria. In this paper, we highlight the potential of the seaweed sector in Indonesia in terms of biodiversity, cultivation, and bioprocessing and discuss the challenges in various aspects, i.e., biology, building capacity, technology, or policy, that currently hinder the expansion of a sustainable Indonesian seaweed industry. Keywords: Seaweed, cultivation, bioprocessing and the challenges Share Link | Plain Format | Corresponding Author (La Ode M. Aslan)

220	Sustainable Aquaculture and Fisheries	ABS-222
Extensive vannamei shrimp culture using Bacillus subtilis, Bacillus licheniformis, and Pseudoalteromonas sp Edeep-1 M Atmomarsono, E Susianingsih, Nurhidayah, Nurbaya, IAK Kadriah Research Center for Fishery, The National Research and Innovation Agency Cibinong, West Java, Indonesia Abstract Abstract. To see if three bacteria probiotics of Bacillus subtilis, B. licheniformis, and Pseudoalteromonas sp Edeep-1 can be used for vannamei culture in the extensive pond, twelve ponds located in Suppa district of Pinrang regency were used. Each pond of about 0.5 ha was stocked with 30,000 pcs of vannamei shrimp postlarvae. Applications tested here were A) The standard probiotics (Brevibacillus laterosporus BT951, Serratia marcescens MY1112, and Pseudoalteromonas sp Edeep-1 BL542- B) The replacement probiotics (Bacillus subtilis BM12, B. licheniformis BM58, and Pseudoalteromonas sp Edeep-1 BL542)- and C) Single use of Brevibacillus laterosporus BT951. There were four replications in each treatment with a culture period was three months. Pond water quality parameters were monitored every two weeks and analyzed at the Research Institute for Coastal Aquaculture and Fisheries Extension Laboratory in Maros, South Sulawesi. The results showed that the survival rate of the shrimp in treatment B was significantly better (P<0.05) than the others. However, shrimp production was not quite different (P>0.05) between A and B treatments. It is concluded that replacement probiotics could be used if the standard probiotic is not available. Keywords: extensive, probiotics, replacement, standard, vannamei shrimp Share Link | Plain Format | Corresponding Author (Muharijadi Atmomarsono)

221	Sustainable Aquaculture and Fisheries	ABS-223
Partial replacement of commercial fish meal with Pterygoplichthys pardalis meal in diets for Oreochromis niloticus and Cyprinus carpio Hasnidar (a*) and Andi Tamsil (a) (a) Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Muslim Indonesia, Jl. Urip Sumoharjo Km. 05 Makassar 90231, Indonesia *hasnidar.yasin[at]umi.ac.id Abstract Amazon sailfin catfish, Pterygoplichthys pardalis is an invasive fish that has affected native fish in several inland waters in Indonesia. This fish is not widely consumed, and its utilization is still very limited, so the population is increasing as well. One effort to utilize it is to process it into fish meal and then as a source of animal protein. The aim of this study was to analyze the use of P. pardalis meal as a substitute for the commercial fish meal on growth performance and feed utilization in tilapia, Oreochromis niloticus and carp, Cyprinus carpio aquaculture. Tilapia and carp, respectively, had total lengths of 4.5 cm and 5.5 cm and body weights of 3.2 g and 2.9 g. The treatments consisted of five, namely diet of P. pardalis meal and commercial fish meal, namely treatment A 0 and 100 %, B 25 and 75 %, C 50 and 50 %, D 75 and 25 % and E 100 and 0 % were maintained for 60 days. The results showed that the treatment of the five diets had a significant effect on body weight gain, daily growth rate, specific growth rate, feed conversion, feed efficiency, and protein efficiency ratio of tilapia and carp. The commersial meal diet was 100 % higher but not different from the P. pardalis meal 25 and 50 % diets. P. pardalis meal can be used as a raw material for artificial feed to replace commercial fish meal up to 50 %, but its use of up to 100 % does not affect the survival rate of tilapia and carp. Keywords: P. pardalis meal- commercial fishmeal- growth performance, Oreochromis niloticus, Cyprinus carpio Share Link | Plain Format | Corresponding Author (Hasnidar Hasnidar)

222	Sustainable Aquaculture and Fisheries	ABS-229
Produksi Padi dan Ikan Mas pada Berbagai Pengelolaan Air, Varietas dan Dosis Pakan pada Sistem Minapadi Makmur1, Amir Yassi2, dan Edison Saade3 1Mahasiswa Pascasarjana Program Studi Sistem-Sistem Pertanian Universitas Hasanuddin, Makassar 2Departement Agronomi, Fakultas Pertanian Universitas Hasanuddin, Makassar 3Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin, Makassar Abstract Makmur. Produksi Padi dan Ikan Mas pada Berbagai Pengelolaan Air, Varietas dan Dosis Pakan pada Sistem Minapadi (dibimbing Amir Yassi dan Edison Saade) Penelitian bertujuan mengetahui metode pengelolaan air, varietas dan dosis pakan serta interaksi ketiganya yang memberikan produksi tinggi padi dan ikan mas pada sistem minapadi. Penelitian dilaksanakan di Mamasa pada Januari - Juni 2022. Rancangan yang digunakan adalah Rancangan Petak-Petak Terpisah, pengelolaan air sebagai petak utama, varietas sebagai anak petak dan dosis pakan ikan mas sebagai anak-anak petak. Pengelolaan air sebagai petak utama yaitu pengairan basah kering (P1) dan pengairan tergenang (P2). Anak petak adalah varietas padi yaitu varietas lokal mamasa (V1) dan Inpari 30 (V2). Anak-anak petak adalah dosis pakan ikan yaitu 1 % bobot ikan (D1), 3 % bobot ikan (D2) dan 5 % bobot ikan (D3). Hasil penelitian menunjukkan intraksi P2D3 memberikan hasil terbaik pada pertumbuhan bobot mutlak (86,00 g) dan laju pertumbuhan spesifik (1,63 %). Interaksi V2D3 memberikan hasil terbaik pada jumlah anakan produktif (13,33 batang), pertumbuhan bobot mutlak (84,33 g) dan laju pertumbuhan spesifik (1,61 %). Interaksi P2V2D3 memberikan hasil terbaik pada pertumbuhan bobot mutlak (103,33 g) dan laju pertumbuhan spesifik (1,78 %). Keywords: Minapadi, pengelolaan air, dosis pakan Share Link | Plain Format | Corresponding Author (Makmur -)

223	Sustainable Aquaculture and Fisheries	ABS-230
Tepung Maggot Hermetia illucens sebagai Sumber Nutrisi dan Pensubtitusi Tepung Ikan pada Pakan Akuakultur Ardiansyah Rusmadi (a), Edison Saade (b*), Haryati Tandipayuk (c) Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin Makassar *edison03081963[at]gmail.com Abstract Maggot atau belatung merupakan larva yang dihasilkan oleh lalat Black Soldier Fly, Hermetia illucens merupakan salah satu pakan yang sangat penting bagi dunia akuakultur. Tepung maggot memiliki kandungan nutrisi yang setara dengan tepung ikan sehingga memiliki potensi sebagai pensubtitusi tepung ikan pada pakan beberapa jenis ikan akuakultur. Maggot mengandung makro dan mikro nutrisi yang cukup tinggi. Kandungan makronutrisi tepung maggot yaitu protein 42-61%, lipid 29-32% lipid, karbohidrat kurang dari 0,05% dan abu 8-11%, sedangkan kandungan mikro-nutrisi maggot berupa asam amino esensial: metionin 0,83%, lysin 2,21%, leusin 2,61%, isoleusin 1,51%, histidine 0,96%, fenilalanin 1,49%, valin 2,23%, arginine 1,77%, treonin 1,41%, dan triptopan 0,59%- asam lemak esensial: C12:0 49,56%, C14:0 7,90%, C16:0 15,17%, C16:1n-7 1,72%, C18:0 1,55%, C18:1 8,32%, C18:2n-6 14,90% dan C18:3n-3 0,88%- mineral P 0,88%, K 1,16%, Ca 5,36%, Mg 0,44%, Mn 348 ppm, Fe 776 ppm, Zn 271 ppm. Pemanfaatan tepung maggot pada pakan ikan akuakultur mampu meningkatkan kecernaan nutrisi, efisiensi pakan, retensi nutrisi dan pertumbuhan yang signifikan bagi ikan lele, nila, bawal, mas, patin bandeng dan mujair. Berdasarkan hal tersebut tepung maggot mampu berpengaruh sangat signifikan bagi usaha budidaya ikan akukaultur. Keywords: maggot, asam amino esensial, asam lemak esensial, mineral, performa pertumbuhan Share Link | Plain Format | Corresponding Author (Ardiansyah Rusmadi)

224	Sustainable Aquaculture and Fisheries	ABS-233
Influence of fresh feed on growth performance snakehead fish (Channa striata) Zainuddin, M I Djawad, M Y Karim, S Aslamyah Faculty of Marine Science and Fisheries, Hasanuddin University Abstract Snakehead fish (Channa striata) is a type of freshwater fish that has an important economic value because its price in the market tends to increase. This type of fish has an albumin content of about 21% and complete essential amino acids. The aim of the research was to determine the best type of fresh feed for optimal snakehead fish growth performance. The research method used was an experimental design with a 4x3 Completely Randomized Design (CRD). The treatments tested were various types of fresh feed (A. Snakeskin gourami Trichopodus pectoralis- B. Silver barb Barbonymus gonionotus- C. The Nile tilapia Oreochromis niloticus and D. pellets (control). The treatments were given three repetitions each. The snakehead fish seeds used were 6- 8 cm stocked at a density of 40 fish/m2 in a tarpaulin pond. The frequency of feeding was done twice a day with a feeding rate of 10% of body weight. The research parameters tested were the glycogen content, the body chemical composition, survival and growth of the test fish. The results showed that the treatment of various types of fresh feed and the control had a significant effect on the body glycogen content of the snakehead fish. The Tukey test results showed that the treatment of fresh Snakeskin gourami feed was significantly lower than that of fresh Silver barb, the Nile tilapia and pellets. Meanwhile, Silver barb feed, the Nile tilapia and pellets were no different. The glycogen content ranged from 32.50-36.21%. Furthermore, various types of fresh feed had the same effect on body chemical composition, survival and growth of snakehead fish. The body protein content ranges from 67.82-69.49%, fat 13.53-14.47%, Nitrogen Free Extract (NFE) 1.83-3.05% and energy 5728.60-5807.04 kcal/kg. The daily growth rate of snakehead fish ranges from 8.95-25.63% per day with survival rates ranging from 35.8% -71.3%. Keywords: Snakehead fish, fresh feed, glycogen, growth, survival rate Share Link | Plain Format | Corresponding Author (Zainuddin Zainuddin)

225	Sustainable Aquaculture and Fisheries	ABS-235
A comparative growth analysis of two different seaweed seeds for cultivation in South Sulawesi Randi Kurniawan (a), Muhammad Maula Razak (b), Agus Jaenuddin (c), Wahyu Saputra (d), Alin Halimatussadiah (e), Jamaluddin Fitrah Alam (f*) a) Faculty of Economy and Business, Hasanuddin University b) LOGOV Celebes c) Yayasan Blue Seed Indonesia (YBSI) d) South Sulawesi Provincial Marine Affairs and Fisheries Department e) Faculty of Economics, University of Indonesia f) Faculty of Marine Science and Fisheries, Hasanuddin University *alamjamaluddin[at]unhas.ac.id Abstract Seaweed plays an important role in the economy of South Sulawesi and Indonesia. To improve the productivity of seaweed in South Sulawesi, the Ministry of Marine Affairs and Fisheries through the Directorate General of Aquaculture Fisheries distributed assistance packages of tissue culture seaweed seeds in seaweed cultivation centers. In this study, the use of tissue culture seaweed seeds was compared with the growth of seeds obtained by the vegetative method by the farmers. This study used primary data obtained from a survey with respondents from seaweed farmers in Pangkep, Bantaeng, and Takalar Regencies. The study results show that farmers who used tissue culture seaweed seeds from the assistance program have higher growth compared to vegetative seeds and lead to a better productivity. Comparing regions, there are also variations of growth, with higher growth for certain regions. This result confirms the tissue culture seaweed seeds assistance program is a success and can further improve the profitability of the farmers. Keywords: Seaweed- Tissue culture- Growth- Seaweed seeds assistance program Share Link | Plain Format | Corresponding Author (Jamaluddin Fitrah Alam)

226	Sustainable Aquaculture and Fisheries	ABS-239
Please Probiotic Bacillus sp. Improved Growth, Survival, and Stress Resistance of Nile tilapia larvae (Oreochromis niloticus Linnaeus,1758)Just Try to Submit This Sample Abstract Irfan Ambas(a*), Dody Dharmawan (a), Trijuno (a), and Rostia (a) Aquaculture Study Program, Department of Fishery, Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia 90245 Abstract Probiotics have been widely used in aquaculture, as an alternative to antibiotics and chemicals. This study aimed to determine the effect of probiotics on the growth, survival, and stress resistance of Nile tilapia larvae. This study examined various probiotics as treatments namely Bacillus sp. (B), Lactobacillus sp. Saccharomyces sp. (C), Lactobacillus casei strain Shirota (D), and a control diet (A). Twenty-five g of commercial fish feed was refined and mixed with 0.5 mL of each probiotic, then air-dried. The experimental organisms were 4-day-old larvae (0.019 g and 1 cm). The larvae were stocked into 3 L jars at a density of 15 ind 2 L and then reared for 30 days. During the rearing period, the feed was given at 5% of biomass body weight 3 times per day. The results indicated that the addition of probiotics significantly (P<0.05) improved the growth and survival of the larvae. Of the three types of probiotics, Bacillus sp. (B) showed higher growth and survival compared to other probiotic treatments (C and D). The highest daily specific growth rate (0.161 %/day) and the highest survival rate (66.67%) were found in treatment (B). In addition, the results of the stress resistance test (salinity stress and starvation) tests, showed that Nile tilapia larvae fed a feed containing the probiotic Bacillus sp (B) had a higher stress resistance. In brief, the probiotics evaluated in this study proved to increase growth, survival, and stress resistance, where Bacillus sp. is the best probiotic for tilapia larvae Keywords: Nile tilapia (Oreochromis niloticus), probiotics, growth, survival, stress resistance Share Link | Plain Format | Corresponding Author (Irfan Ambas)

227	Sustainable Aquaculture and Fisheries	ABS-241
Please JusMUTU IKAN KEMBUNG LELAKI (RASTRELLIGER KANAGURTA) SEGAR YANG DIPASARKAN DI PANGKALAN PENDARATAN IKAN PAOTERE BERDASARKAN PARAMETER KIMIAWIt Try to Submit This Sample Abstract Andi Fadhil Al Farid (a), Fahrul (a*), Nursinah Amir(a) Program Studi Pemanfaatan Sumberdaya Perikanan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin Abstract Penelitian ini bertujuan untuk mengetahui mutu ikan Kembung Lelaki (Rastrelliger kanagurta) segar yang dipasarkan di pangkalan pendaratan ikan Paotere berdasarkan parameter Kimiawi. Pengujian parameter kimiawi yang meliputi pH (derajat keasaman), Histamin, dan Total Volatile Base (TVB)., pengamatan organoleptik, dan pengujian suhu. Data hasil pengujian yang telah didapatkan kemudian dijelaskan secara deskriptif dan ditampilkan dalam bentuk tabel dan grafik. Hasil pengujian yang dilakukan menunjukkan adanya penurunan mutu ikan yang diuji seiring waktu sampling. Pada Sampling 1, nilai rata rata yang dipatkan pada parameter kimiawi yaitu pH 6,99, Histamin 17,45 mg.100g-1, dan TVB 63,08 Mg-N.100g-1. serta rata rata nilai Organoleptik pada jam 10 yaitu 6,83, pada jam 12 yaitu 6, dan pada jam 2 yaitu 4,17. Dan nilai rata rata suhu adalah 23,47 celcius. selanjutnya Pada Sampling 2, nilai rata rata yang dipatkan pada parameter kimiawi yaitu pH 6,7, Histamin 14,24 mg.100g-1, dan TVB 74,22 Mg-N.100g-1. serta rata rata nilai Organoleptik pada jam 10 yaitu 6,75, pada jam 12 yaitu 5,83, dan pada jam 2 yaitu 4,16. Dan nilai rata rata suhu adalah 24,42 celcius. Dan Pada Sampling 3, nilai rata rata yang dipatkan pada parameter kimiawi yaitu pH 6,6, Histamin 14,21 mg.100g-1, dan TVB 66,7 Mg- N.100g-1. serta rata rata nilai Organoleptik pada jam 10 yaitu 6,67, pada jam 12 yaitu 6, dan pada jam 2 yaitu 4,5. Dan nilai rata rata suhu adalah 28,05 celcius. sehingga dapat disimpulkan bahwa mutu ikan kembung lelaki yang didaratkan di PPI kurang baik Keywords: Ikan Kembung Lelaki, TVB, Histamin, dan pH Share Link | Plain Format | Corresponding Author (Fahrul Fahrul)

228	Sustainable Aquaculture and Fisheries	ABS-242
QUALITY OF FRESH MALE MACKEREL (RASTRELLIGER KANAGURTA) MARKETED AT THE PAOTERE FISH LANDING BASE IN MAKASSAR CITY BASED ON MICROBIOLOGICAL PARAMETERS A. Muh. Alief Rafli Abdullah1), Fahrul1), Nursinah Amir1) Fisheries Department, Faculty of Marine Science and Fisheries, Universitas hasanuddin Abstract Research on the quality of fresh male mackerel (Rastrelliger kanagurta) marketed at the Paotere fish landing base in Makassar City on microbiological parameters. The purpose of this study was to determine the quality of fresh male mackerel (Rastrelliger kanagurta) marketed at the Paotere fish landing base in Makassar City by microbiology. Microbiological test parameters include Total Plate Number (ALT) and coliform as well as measuring supporting data, namely organoleptic, temperature and pH. The results showed that the Total Plate Number at week 1 obtained an ALT value of 14.000.000 colonies.g-1 and increased to 2.000.000 colonies.g-1. In week 2 the ALT value was 4000.000 colonies.g-1 and continued to increase up to 10.000.000 colonies.g-1. And in week 3 the ALT value was 11.000.000 colonies.g-1 and continued to increase up to 20.000.000 colonies.g-1. For coliform bacteria in week 1,2, and 3, respectively the values were 93-460 APM.g-1, 36-1100 APM.g-1, and 29-460 APM.g-1. From the results of the study it can be concluded that there is an influence on microbiological quality which continues to increase with increasing hours. From the two tests conducted, it can be seen that male mackerel at 10 to 14 hours is no longer safe for consumption because it exceeds the maximum SNI limit. Keywords: ikan kembung lelaki, ALT, bakteri coliform Share Link | Plain Format | Corresponding Author (Fahrul Fahrul)

229	Sustainable Aquaculture and Fisheries	ABS-247
Performance of Growth and Survival Rate Silver Pompano (Trachynotus blochii) Seed after Additional Amino Acid Lysine in Feed H Minjoyo1, S Saputra1, AR Rivaie1, S Antoro1, SB Dhoe1, A Prihaningrum2, MA Suprayudi3 1Research Center for Fishery, National Research and Innovation Agency, Indonesia 2Lampung Main Center of Marine Aquaculture - Ministry of Marine Affairs and Fisheries Indonesia, Indonesia 3Aquaculture Department. Fisheries and Marine Science, Bogor Aqricultural University, Indonesia Abstract Lysine is one of the essential amino acids and is important factor in the feed that affects the growth of fish. The objective of the present study is to determine the optimum percentage of lysine dietary in feed that able to produce maximum growth performance of silver pompano seed. These studies were done with four different treatments of additional lysine levels, ie- treatment A: 0.15%, treatment B: 0.35 %, treatment C: 0.55 % and K 0 % for control. The study design was completely randomized design with 4 treatments, each consisting of 3 replications. The results showed that feeding with different levels of lysine had no significant effect on body weight gain, feed consumption, absolute weight, feed conversion ratio or survival rate except for specific growth rate and lysine retention, which had a significant effect on fish fed treatment A (0.15%) or containing 3.39% lysine in feed. Keywords: Silver Pompano, Lysine, Feed, Growth Share Link | Plain Format | Corresponding Author (Suryadi Saputra)

230	Sustainable Aquaculture and Fisheries	ABS-248
Growth Performance and Survival Rate of Silver Pompano, Trachinotus blochii (Lacepede, 1801) Seed after Additional Amino Acid Lysine in Feed H Minjoyo1, S Saputra1, AR Rivaie1, S Antoro1, SB Dhoe1, A Prihaningrum2, MA Suprayudi3 1Research Center for Fishery, National Research and Innovation Agency, Indonesia 2Lampung Main Center of Marine Aquaculture - Ministry of Marine Affairs and Fisheries Indonesia, Indonesia 3Aquaculture Department. Fisheries and Marine Science, Bogor Agricultural University, Indonesia Abstract Abstract. Lysine is one of the essential amino acids and is important factor in the feed that affects the growth of fish. The objective of the present study is to determine the optimum percentage of lysine dietary in feed that able to produce maximum growth performance of silver pompano seed. These studies were done with four different treatments of additional lysine levels, ie- treatment A: 0.15%, treatment B: 0.35 %, treatment C: 0.55 % and K 0 % for control. The study design was completely randomized design with 4 treatments, each consisting of 3 replications. The results showed that feeding with different levels of lysine had no significant effect on body weight gain, feed consumption, absolute weight, feed conversion ratio or survival rate except for specific growth rate and lysine retention, which had a significant effect on fish fed treatment A (0.15%) or containing 3.39% lysine in feed. Keywords: Silver Pompano, Lysine, Feed, Growth Share Link | Plain Format | Corresponding Author (Herno Minjoyo)

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