Semi-Polar Fraction of Soft Coral Nepthea Sp.: Chemical Profile, Antioxidant and Cytotoxic Potencies Sahidin1, Baru Sadarun2, Nur Syifa Rahmatika3, Agung Wibawa Mahatva Yodha4, Adryan Fristiohady1
1Faculty of Pharmacy, Universitas Halu Oleo, Kendari, INDONESIA
2Faculty of Marine Sciences and Fisheries, Universitas Halu oleo, Kendari, INDONESIA
3Faculty of Medicine, Universitas Halu Oleo, Kendari, INDONESIA
4Health Polytechnics of Bina Husada, Kendari, INDONESIA
Abstract
The propose of this research is to study on chemical and pharmaceutical aspects of soft coral Nepthea sp. The sample was extracted by ethylacetate (EAE) and then fractionated using vacuum liquid chromatrography (VLC). The chemical content was analyzed by phytochemical screening, LC-MS/MS analysis, Total Phenolics Content (TPC) and Total Flavonoids Contents (TFC). Antioxidant potency was evaluated by DPPH and ABTS methods. Cytotoxicity was analyzed by MTT assays. The result showed that the fractionation produced six fractions (1-6). Fraction 3 and 4 were semi-polar compounds, which contained terpenoids, phenolics and alkaloids. Based on LC-MS/MS data, the identified semi-polar compounds consist of 3-acetyl-3,4-dihydro-5,6-dimethoxy-2(1)H-benzopyrone and oxyphyllenone B from Fraction 3 and 1-(4-Hydroxy-3-methoxy)-phenyl-1,2,3-propanetriol, digiprolactone, petasitolone, δ--Humulene, rengyolester, and piperolactam-C9:1(8E) for Fraction 4. The antioxidant potency of fraction 4 was better than Fraction 3, which is supported by TPC Fraction 4>Fraction 3 with active antioxidant category for Fraction 4 and Fraction 3 is moderatery active antioxidant category. Based on IC50 value, Fraction 3 is active category and Fraction 4 is moderately active category against Breast Cancer cell lines MCF-7. The semi-polar fraction of Nepthea sp. can be developed as raw material for antioxidant and anticancer.
