Sargassum aquifolium juice induces IL-4 expression in DM 2 rats
Muhamad Firdaus1*, Yoga Dwi Jatmiko2, Retno Tri Astuti1, Herlina Pratiwi3, and Ahmad Faris Priambodo1

1Faculty of Fisheries and Marine Sciences, Universitas Brawijaya, Malang, Indonesia
2Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia
3Faculty of Veterinary, Universitas Brawijaya, Malang, Indonesia

Abstract

Hyperglycemia decreases the expression of anti-inflammatory cytokines. Sargassum sp contains compounds that their bioactivity as anti-hyperglycemia. This study evaluated the Sargassum aquifolium extract to express anti-inflammation in Diabetes Mellitus (DM) 2 rats model. S. aquifolium was obtained from Ekas Bay, Lombok Island. A Male (Rattus norvegicus) was fed an atherogenic diet for 16 weeks and then injected peritoneally with streptozotocin (20 mg/dL). This study was grouped into healthy, diabetes + aquades, diabetes + 1,5 mL of S. aquifolium juice, diabetes + 3,0 mL of S. aquifolium juice, and diabetes + 4,5 mL of S. aquifolium juice. The parameter of this study was IL-4 expression. The relative percentage of IL-4 from Peripheral Blood Mononuclear Cells (PBMC) was measured using flow cytometry. The result showed that IL-4 expression in healthy groups was significantly higher than in diabetic groups. IL-4 expression in the diabetic group administered with 3 mL of S. aquifolium juice was significantly higher than in the diabetic group administered with aquadest or other volumes of S. aquifolium juice. Prevention of increased blood glucose by S. aquifolium juice reduces inflammation of diabetes. In conclusion that 1,5 mL of S. aquifolium juice increased IL-4 expression and prevented inflammation in diabetes.

Keywords: S. aquifolium juice, diabetes mellitus type 2, inflammation, Interleukin-4

Topic: Marine and Fisheries Biotechnology