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121	Marine and Fisheries Biotechnology (International)	ABS-22
Application of 17alpha-methyltestosterone to enhance broodstock maturation of the domesticated black tiger shrimp (Penaeus monodon): A comparative study of injection method and oral method R Rosmiati, S Lante, A Parenrengi, E Suryati, A Tenriulo, and A Nawang 1Research Institute for Brackishwater Aquaculture and Fisheries Extension, Maros, South Sulawesi, Indonesia 2National Research and Innovation Agency, Maros, South Sulawesi, Indonesia Abstract The effects of 17alpha-methyltestosterone on spermatophore quality of tiger shrimp Penaeus monodon were investigated using injection and oral method. The experiment was designed to compare the oral administration group and injection group with two replications at 38-day experimental period. Captive male broodstock of tiger shrimp weighing 64.8-71.5 g collected and stored in a 10,000 m3 concrete tank (20 shrimp/tank). Male were injected and oral administrated with 17alpha-methyltestosterone at the dose of 300 ng/100 g body weight. The injection of 17alpha-methyltestosterone shows the percentage of mature broodstock and the percentage of normal spermatozoa count 1.07 and 1.38 higher and significantly differs from oral administration through mixing in feed at the maturation stage. However, at the time of the 1st re-maturation and 2nd re-maturation of both the methods, the number of mature broodstock, the weight of the spermatophores, the number of spermatozoa and the percentage of the normal spermatozoa did not show any difference. Based on the comparison of the two methods used, it is obtained that the administration of the hormone 17alpha-methyltestosterone can be done both through mixing in feed and through injection, but the administration of hormones through injection gives better results that the broodstock matures first simultaneously. Keywords: Captive tiger shrimp, 17alpha-methyltestosterone, Spermatophore quality, mature broodstock, Normal spermatozoa Share Link | Plain Format | Corresponding Author (Rosmiati Rosmiati)

122	Marine and Fisheries Biotechnology (International)	ABS-286
Molecular docking analysis of selected natural products from Halymenias sp. and Laurencia sp seaweeds against Plasmepsins as antimalarials. Asmi Citra Malina A.R Tasakka*1, Israini Wiyulanda Iskandar2 , Sulfahri3, Eko Agus Suyono4, Eko nurcahya dewi5, Mochammad Yuwono6, Kasmiati1 , Elmi Nurhaidah Zainuddin1, Marlina Achmad1, &#8234-Muhammad Iqbal Djawad1, Jamaluddin Fitrah Alam1&#8236-&#8236-, Widyastuti Umar&#8236-&#8236-1, and Andi Alya Yusriyyah2&#8236-&#8236-&#8236-&#8236-&#8236-&#8236- 1. Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia 2. Center for Excellence in Science and Technology of the Development and Utilization of Seaweed (PUI-P2RL) 3. Faculty of Mathematics and Natural Science, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia 4. Faculty of Biology, Universitas Gadjah Mada 5. Faculty of Fisheries and Marine Sciences, Diponegoro University 6. Faculty of Pharmacy, Airlangga University *corresponding author : citra[at]unhas.ac.id Abstract Malaria is one of the most important public health problems worldwide, with nearly half of the global population exposed to the risk of contamination. The disease is found in 91 countries, mostly in the tropics and subtropics of the planet. There are several previous research that identifies Plasmepsins as a potential target to develop novel antimalarial drugs from the malaria parasite Plasmodium that play a role in the breakdown of globin into amino acids. Given the above, it is important to find novel and effective drugs that can decrease this disease, especially from natural products such as medicine. Seaweed is a potential source of bioactive compounds to be used as antimalarials such as species from the genera Laurencia and Halymenia. This recent study has studied the molecular docking approach to identify the potential of Halymenias sp. and Laurencia sp against Plasmepsin by using PyRx 0.8 software. It showed that the compounds in Halymenias sp. and Laurencia sp were able to react and inhibit the action of plasmepsin, seen from the binding affinity value which was quite small. ,3, this value is higher than the two bioactive compounds in seaweed, namely Stigmasterol and p-hydroxybenzaldehyde which have binding affinity values of -8.5 and 6.5, respectively. Judging from this, the compounds contained in Laurencia and Halymenia have potential as candidates for antimalarial drugs. Keywords: Halymenias sp., Laurencia sp., Malaria, Natural Products, Plasmepsins Share Link | Plain Format | Corresponding Author (Israini Wiyulanda Iskandar)

123	Marine and Fisheries Biotechnology (International)	ABS-31
Chemical profile, toxicity, cytotoxicity and antioxidant potencies of ethylacetate extract of soft coral Lobophytum sp. growing in South East Sualwesi sea Sahidin I1, Adryan Fristiohady1, Muhammad Hajrul Malaka1, Baru Sadarun2, Nur Syifa Rahmatika3, Agung Wibawa Mahatva Yodha1,4, Andini Sundowo5 1Faculty of Pharmacy, Universitas Halu Oleo, Kendari, INDONESIA 2Faculty of Marine Sciences and Fisheries, Universitas Halu oleo, Kendari, INDONESIA 3Faculty of Medicine, Universitas Halu Oleo, Kendari, INDONESIA 4Health Polytechnics of Bina Husada, Kendari, INDONESIA 5Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten, INDONESIA Abstract Soft coral of Lobophytum sp. grows a lot in the seas of South East Sulawesi, Indonesia. However, no information has been found regarding the study of chemical and pharmaceutical aspects of this genus fom this area. Therefore, the purpose of this article is to present the results of a general study on the pharmaceutical and chemical aspects of Lobophytum sp. The sample collected from the water of Saponda Island, than extracted by ethylacetate. The chemical content was analyzed using phytochemical tests and LCMSMS. Antioxidant potency was evaluated by DPPH radicals and ABTS methods, cytotoxicity towards MCF7 cell line using MTT assays, and toxicity by BSLT. The results showed that the ethylacetate extract has potential as antioxidant and cytotoxic against MCF7 cell lines which indicated by IC50 value. The antioxidant potential was revealed by IC50 (g/L) 84.99 (DPPH) and 67.99 (ABTS). In addition, the cytotoxicity presented by IC50 (g/L) 37.35 and 50.07 for BSLT. Those activities supported by the qualitative phytochemical screening that exhibited the extract contains terpenoids, steroids, alkaloids and phenolics compounds. LCMSMS data indicated that ethylacetate extract has arachidonic acid, edultin, fragransol D, neociwujiaphenol, pluviatelol and some unidentified compounds with molecular formulas C21H26O8, C23H47N3O, C32H51NO7, C31H47NO3, C33H51NO3. Keywords: Lobophytum sp., ethylacetate extract, chemical profile, antioxidant, toxicity Share Link | Plain Format | Corresponding Author (I. Sahidin)

124	Marine and Fisheries Biotechnology (International)	ABS-52
TEST THE EFFECTIVENESS of Porphyridium aerugineum AS VIBRIOSIS CONTROLLER IN SHRIMP PONDS Ince Ayu K.Kadriah*1, Endang Susianingsih1, Nurhidayah1, Nurbaya1 and Herlinda3 1National Research and Innovation Agency 2Technic Faculty, Makassar State University, Makassar, Indonesia Abstract Bacterial disease control in shrimp ponds is currently taking a more environmentally friendly approach. This study aims to find out the effectiveness of Porphyridium aerugineum as a controller of Vibriosis in ponds. This research was carried out at with two treatments with three replications each. The first treatment is the administration of whole-cell microalgae P. aerugineum and the second treatment is Control without microalgae administration. Tigers shrimp was stocked at the stadia PL 12 (density of 8 shrimp fry/m2). The study used 6 ponds measuring 10m x 25m with a water depth of 80cm. Microalgae is given once a week. A sampling of pond water and sediment is carried out once in two weeks. The results showed that the addition of wholecell microalgae P.aerugineum in ponds can reduce the ratio of Total Vibrio bacteria and Total plate count in ponds water but not in pond sediments. In ponds fed microalgae P.aerugineum, shrimp survival rate and production were higher than those of in controls. The results of this study showed that the administration of microalgae as an additional supplement in ponds gave better results compared to the control. Keywords: Anti Quorum Sensing, Anti Vibrio, Diatom, Porphyridium aerugineum, Tiger shrimp Share Link | Plain Format | Corresponding Author (Ince ayu Kadriah)

125	Marine and Fisheries Biotechnology (International)	ABS-73
UV absorbing substances in the Indonesian starfish Archaster typicus Delfly B Abdjul (1,2*), Magie M Kapojos (3), Hiroyuki Yamazaki (2), Michio Namikoshi (2)2 (1) North Sulawesi Research and Development Agency, Manado, Indonesia *booby_abdjul[at]yahoo.com (2) Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, Japan (3) Faculty of Science and Technology, Prisma University, Manado, Indonesia Abstract Marine organisms living in the shallow water ecosystems are potentially exposed to the highest and harmful levels of UV radiation. The starfish Archaster typicus lives in the shallow water habitat and, therefore, is exposed to the highest level of UV radiation, suggesting that this starfish contains UV absorbing compounds to diminish the direct and indirect damaging effects of environmental UV radiation. This study aimed to identify the UV absorbing compounds in the Indonesian starfish A. typicus. Two UV absorbing compounds, palythine (1) and asterina-330 (2), were isolated from A. typicus collected at Tasik Ria, North Sulawesi, Indonesia. The structures of these compounds were assigned on the basis of their spectroscopic data and confirmed by comparing these data with those for the reported values. This is the first report of isolation UV absorbing compounds from A. typicus. Keywords: UV absorbing- Archaster typicus- Palythine- Asterina-330- North Sulawesi-Indonesia Share Link | Plain Format | Corresponding Author (Delfly Abdjul)

126	Marine and Fisheries Biotechnology (International)	ABS-102
Application of dsRNA VP24 WSSV by Oral Administration at Different Larval Stages of Tiger Shrimp Penaeus monodon A. Tenriulo(a*), A. Parenrengi(a), S. Lante(a), E. Suryati(a,b), R.Rosmiati(a), and Agus Nawang(a) (a)Research Institute for Brackishwater Aquaculture and Fisheries Extension, Maros, Sulawesi Selatan, Indonesia *Corresponding author: anditenriulo[at]yahoo.com (b)National Research and Innovation Agency, Maros, Sulawesi Selatan, Indonesia Abstract This study aimed to evaluate the effect of dsRNA VP24 application on different stages of larvae. The cloned recombinant bacteria carrying the construct of promoter T7VP24 grew in LB media for mass production. The bacteria were inactivated using the heat-killed bacteria method by immersion at 80o C for 5 minutes. The inactivated bacteria were mixed with larval commercial feed. The nauplii were produced from broodstock from Aceh waters and reared until postlarva 12 (PL12). The feed containing the dsRNA vaccine was applied to a different stage of larva, i.e: starting from zoea 1 (A), mysis 1 (B), PL 1 to the PL 12 (C), and control without dsRNA (D). The PL 12 were challenged with WSSV by immersion method and morphological characters were assessed. Results showed that inactivated bacteria were effectively done by immersion at 80o C for 5 minutes without damaging of dsRNA construct in the plasmid. The survival rate was significantly influenced by different stages of larvae (P 0.05), in which the highest survival (26.0%) was obtained from mysis. The highest value of morphological characters (92.3) was also inhibited in the mysis. The results suggested that the dsRNA vaccine for larvae could be started to be applied in the mysis stage. Keywords: dsRNA, larval stage, P.monodon, VP24-WSSV Share Link | Plain Format | Corresponding Author (Andi Tenriulo)

127	Marine and Fisheries Biotechnology (International)	ABS-144
Prevention of Vibriosis using wholecell microalgae in tiger shrimp hatcheries Nurbaya1, Ince Ayu K.Kadriah*1, Muharijadi Atmomarsono1, Muliani1 and Hilal Anshary2 1Nasional Research and Innovation Agency 2Faculty of Fisheries and Marine Science, Hasanuddin University Abstract Vibriosis is the main cause of mass mortality in a tiger shrimp hatchery.To see whether whole-cell microalgae Porphyridiumsp and Porphyridiumaerugineumcan be used for preventingvibriosis in tiger shrimp larvae, this study uses a completely randomized design with three treatments and three replications each was carried out in the RIBAFE hatchery in Awarange, Barru. Three treatments tested here were A) Control (without additional microalgae), B)Porphyridiumaerugineum, and C)Porphyridium sp. These two microalgae with a stocking density of about105cells/mLwere administered in the rearing tank at the shrimp stage of Zoea1, Mysis1, PL2, PL6, and PL10.The density of microalgae was observed every day from Zoea-2 to PL10, whilethe density of bacteria was measured at the above stages. At the end of the fry rearing period (PL12), a challenge test was carried out using the bacteria Vibrioparahaemolyticus with a density of 106 CFU/mL.The results showed that the administration of P. aerugineum could suppress the population of Vibrio bacteria and the ratio of Vibrio bacteria to common bacteria.However, the best survival rate of tiger shrimp postlarvaewas obtained by treatment C(Porphyridiumsp). Keywords: Mikroalgae, Porphyridium sp, Porphyridium aerugineum, Vibriosis Share Link | Plain Format | Corresponding Author (Nurbaya Nurbaya)

128	Marine and Fisheries Biotechnology (International)	ABS-152
Utilizing Pterygoplichtis pardalis fish bone as material of bone graft for bone remodelling in periodontitis Nursyamsi Djamaluddin, Nurlindah Hamrun, Nursinah Amir, Muhammad Nur Amir, Andi Apriliiqa Megumi Adhila Larasati, Rahma Sania Syahrir, Shaffati Shaffa Universities Hasanuddin Abstract Periodontitis is a disease that results in tooth loss due to alveolar bone resorption. Bone grafting can be used to restore damage to the periodontal tissues of the teeth. The calcium, phosphate, and hydroxyapatite concentration of plecos catfish (Pterygoplichtis pardalis) is over 70%. This has the potential to be developed into a bone transplant material that is an alternative to hydroxyapatite. Aim: The purpose of this study was to determine the quality of HA of P. pardalis fish bone as a bone graft material during the bone remodeling stage, as well as the effect of P. pardalis fish bone as a bone graft material during the bone formation phase on the bone remodeling process. Method: This is an experimental study using a pre and post test only group design, with four groups of test animals: group one received no treatment- group two received 100 percent HA fish P. pardalis hydrothermal process- group three received 100 percent HA beef/bovine bone- and group four received 50 percent HA fish P. pardalis hydrothermal method and 50 percent HA beef/bovine bone. After that, the samples were subjected to an ELISA test, a radiography test, and a histopathological test. Results: The HA powder of P. pardalis fishbones is non-toxic, high in PO43- , CO32- and OH-, increasing of TNF-&#945- shown as inflammation condition of periodontal tissue disease, defect is not covered perfectly by radiograph, and HA of P. pardalis bonefish can stimulate amount of osteoblast cells. Conclusion: P. pardalis fish bone HA has the potential to be used as a bone graft. Keywords: bone remodelling, bone resorption, hydroxyapatite, periodontitis, plecos catfish bone Share Link | Plain Format | Corresponding Author (Nursyamsi Djamaluddin)

129	Marine and Fisheries Biotechnology (International)	ABS-154
Use of Sonneratia alba leaf extract solution for White Spot Syndrome Virus prevention in tiger shrimp postlarvae Nurhidayah, Muliani, Nurbaya, Muharijadi Atmomarsono National Research and Innovation Agency Abstract Abstract. White Spot Syndrome Virus is one of the leading causes of mass mortality in tiger shrimp culture. This study aimed to determine the effect of different immersion times in 1% Sonneratia alba extract on the survival of tiger shrimp Postlarvae after infection with WSSV. The study used a completely randomized design with treatments, namely A) 12 hours of immersion- B) 24 hours- C) 36 hours- D) 48 hours- E) 60 hours- F) 72 hours- G) Positive control (infected with WSSV without mangrove extract)- and H) Negative control (without WSSV infection and mangrove extract), with three replications each treatment. Stocking density of tiger prawn postlarvae 20 pcs/jar. All immersion methods were performed before WSSV infection (concentration 0.1%). They were feeding three times a day, 20% of the total biomass per day. Post-larvae mortality of tiger prawns was observed every day for eight days. Water quality parameters, including Total Organic Matter, NH3-N, and NO2-N, were measured at the beginning and end of the study. The results showed the highest survival rate (85.0%) in treatment F, while the lowest (0%) was positive control (treatment G). The more extended immersion in the herbal extracts increased the shrimp^s protection against WSSV. Keywords: Sonneratia alba, mangrove extract, immersion, WSSV prevention, tiger shrimp Share Link | Plain Format | Corresponding Author (Nurhidayah Nurhidayah)

130	Marine and Fisheries Biotechnology (International)	ABS-159
Reproductive performance of male tiger prawns (Penaeus monodon) fed trash fish and fermented sea cucumber (Holothuriadae) offal- study of gonadal morphology and histology Andi Aliah Hidayani (a), Yushinta Fujaya (a*), Achmad Radhifan Imam Wahyudi Duni (a), Muh Darhan Hibaturrahman (a), Waiho Khor (b), Hanafiah Fazhan (b), Benny Diah Madusari (c), Haruna (d) a) Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Tamalanrea, Makassar, South Sulawesi Indonesia. b) Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, 21030, Terengganu, Malaysia c) Fisheries Faculty, Pekalongan University, Jl. Sriwijaya 3, Bendan, Pekalongan, Central Java Indonesia d) Takalar Brackish Water Cultivation Fisheries Center, Mappakalompo Village, Galesong District, Takalar Regency, South Sulawesi Indonesia Abstract This study aimed to compare the effect of various doses of fermented sea cucumber (Holothuridae) offal in trash fish on the development of gonads and sperm of tiger prawns (Penaeus monodon). There were 36 adult male tiger prawns with an average weight of 83.61 g used in this study. Completely Randomized Design (CRD) with 4 treatments and 3 replications used in this study. treatment A was feeding trash fish without fermented sea cucumber as a control, treatment B was feeding trash fish mixed with 25 ml fermented sea cucumber innards, treatment C was giving trash fish feed mixed with 50ml fermented sea cucumber, and treatment D was feeding trash fish mixed with 75ml fermented sea cucumber. The development of gonads and spermatozoa was observed morphologically and histologically. The results showed that feeding trash fish mixed with fermented sea cucumber offal with different doses had no significant effect (p>0.05) on the gonadal maturity index of male tiger prawns but had a significant effect (p, <0.05) on the number of spermatozoa. The highest number of spermatozoa in terminal ampule was obtained in treatment C, namely 114.83 x 106, and the lowest yield was obtained in treatment A that is 30.75 x 106. Keywords: sea cucumber, Penaeus monodon, gonad, spermatozoa, histology, morphology. Share Link | Plain Format | Corresponding Author (Andi Aliah Hidayani)

131	Marine and Fisheries Biotechnology (International)	ABS-168
REGENERATION OF KAPPAPHYCUS ALVAREZII SEAWEED RESULTING FROM THE TRANSFORMATION OF THE PACS GENE (Citrate synthase) TO INCREASE RESISTANCE TO ENVIRONMENTAL CONGESTION IN FLOATING NET CAGES IN PANGKEP WATER Emma Suryati1,2), Andi Tenriulo1,2)Rosmiati1,2) , Andi Parenrengi1,2), and Sri Rejeki HM1,2 1) Research Institute for Brackhiswater Aquaculture and Fisheries Extention 2) Center of Fisheries Research, National Research and Innovation Agency, Maros, South Sulawesi Abstract The transformation of the PaCs (Citrate synthase) gene in seaweed K.alvarezii has been successfully performed in vitro. To improve the survival of the transformation of the Citrate synthase seaweed gene, it is carried out: (1) Regeneration of the results of transformation in culture chamber in the laboratory- (2) Acclimatization in green net (MS 100 um) size 50x50x50 cm, density 200 explants maintained for 2 weeks, transferred to the blue net with a size of 50x50x50 cm (MS 200 um) density 200 explants maintained for 4 weeks, then maintenance by the longline method using a double rope with a distance of 15 Cm on floating net cages. Measurement of weight, length, and water quality, is carried out at intervals of 2 weeks. Analysis of PaCs gene integration with PCR using Primary PaCS-F and PaCS-R. The results showed that the DGR in the culture chamber was 0.11% to 0.18%/day. Acclimatization on green nets indicates a DGR of 0.62 to 1.3% / day, and on blue nets, DGR increases to 2.18% /day. Seaweed enlargement using the longline method shows a DGR of 1.42-4.3%/day. The results of the integration of the Citrate synthase gene in seaweed showed the band at a position corresponding to the promoter fragments of 1300 bp PaCS-F and PaCS-R in the CaMV 35S vector. Based on these results the PaCS gene has been successfully inserted into the seaweed K.alvarezii Keywords: Acclimatization, Kappaphycus alvarezii, floating net cage, long line, growth rate, gene PaCS (Citrate synthase) Share Link | Plain Format | Corresponding Author (Emma Suryati)

132	Marine and Fisheries Biotechnology (International)	ABS-169
The Investigated of Antioxidant Properties and Total Phenolic Content of Seagrape Caulerpa racemosa (Forsskal) J. Agardh at Intertidal Zone of Lhok Bubon, Aceh Mohamad Gazali, Muhammad Syafik, Amir Husni, Nurjanah, Neviaty P. Zamani, Zuriat, Endah Anisa Rahma, Alaudin, Hayatun Nufus, Rina Syafitri1 Department of Marine Science, Faculty of Fisheries and Marine Science Teuku Umar University, West Aceh, Indonesia Student of Department of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University, West Aceh, Indonesia Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia Department of Aquatic Product Technology, Faculty of Fisheries and Marine Science, IPB University, Bogor, Indonesia Department Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University, Bogor, Indonesia Department of Fisheries, Faculty of Fisheries and Marine Science, Teuku Umar University, Bogor, Indonesia Department of Marine Science, Faculty of Fisheries and Marine Science Teuku Umar University West Aceh, Indonesia Department of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University, West Aceh, IndonesiaDepartment of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University, West Aceh, Indonesia Department of Agribusiness, Faculty of Agriculture, Teuku Umar University, West Aceh, Indonesia Abstract Caulerpa racemosa know as sea grape is green seaweed that found in the Intertidal Zone of Lhok Bubon, West Aceh but this green seaweed has not yet been utilized by the coastal community. The aim of this study was to determine the antioxidant activity of C. racemosa extract from intertidal zone of Lhok Bubon. In research method, the sample of C. racemosa were collected from Intertidal Zone of West Aceh and crude extracts were prepared using ethanol, ethyl acetate, and n-hexane. The yield and phytochemical assay were measured then followed by the in vitro antioxidant assays and total phenolic content. The result showed the highest yield was found in the methanol (6.07 of percent), followed by ethyl acetate extract (3.79 of percent) and n-hexane (0.75 of percent). C. racemosa crude extract have detected phenolic, flavonoid, saponin and steroid compounds. The antioxidant activity measurement with DPPH method showed the methanol extract had higher activity with value of 26.11 compared than ethyl acetate crude extract with value of 49.42 and n-hexane extract 59.42. It is corroborated with the total phenolic content correlation with antioxidant activity as much as 99 of percent of antioxidant activity contributed from phenolic compound. Some of these bioactive compounds possess a new antioxidant agent with interesting potential for the nutraceutical industry. Keywords: Antioxidant, bioactive compound, Caulerpa racemosa, West Aceh Share Link | Plain Format | Corresponding Author (Mohamad Gazali)

133	Marine and Fisheries Biotechnology (International)	ABS-173
Development of recombinant protein adjuvants based on Vibrio harveyi flagellin gene in Epinephelus coioides Andre Giovanni(a), Shun Maekawa(a,f*) , Pei-Chi Wang(b,c,e) , Shih-Chu Chen(a,b,c,d,e*) a)International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan b)Southern Taiwan Fish Diseases Research Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan c)Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan. d)Research Center of Animal Biologics, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan e)Research Center for Fish Vaccine and Diseases, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan f)General Research Service Centre, National Pingtung University of Science and Technology, Pingtung, Taiwan Abstract Vibrio harveyi is a gram-negative bacterium that has flagella which are useful for locomotion. This bacterium is an important pathogen causing vibriosis in shrimp and some marine fish, including the orange-spot grouper (Epinephelus coioides) can become infected with these pathogens. In V. harveyi virulent genes such as chitinase, exotoxin, metalloprotease, serine protease, hemolysin and flagellin genes have been identified. The toxin produced by Vibrio harveyi flagella can be a source of infections in fish. The purpose of this research is to look at how gene responses in orange-spot grouper (Epinephelus coioides). Binding of VhFliA to TLR5 could activate the innate and adaptative immune system to stimulate production of cytokines. This study used VhFliA and mutants gene and analyzed cytokine (IFN&#947-, IL-1&#946-, IL-6, IL-8, and IL-10) and also IFN&#947- downstream gene (MHC class I, MHC class II, CD4, CD8) immune response gene. Determine the responsiveness of Vibrio harveyi flagellin (VhFliA) gene expression in the spleen and head kidney of grouper. Total RNA was extracted from the spleen and head kidney, and qRT-PCR was used to analyze it. The post-injection observation period is repeated several times. There are four different treatments: EGFP as a control, wild-type VhFliA, and two mutagenesis of wild-type VhFliA (&#8710-MH-VhFliA and &#8710-D0MH-VhFliA). We^ve found an upregulation in cytokines and downstream genes. In this study, we identified and characterized V. harveyi flagellin A (VhFliA) gene. We investigated the in vivo activities of recombinant VhFliA protein and its mutant forms (&#8710-MH-VhFliA and &#8710-D0MH-VhFliA) in grouper. This study will provide the development of epidemic prevention strategies in aquaculture species using recombinant VhFliA and its mutants protein Keywords: Vibrio harveyi- flagellin- toll-like receptor 5- Immune response- Epinephelus coioides Share Link | Plain Format | Corresponding Author (Andre Giovanni)

134	Marine and Fisheries Biotechnology (International)	ABS-185
Antibacterial agent of n-hexane and ethanol Polycarpa aurata extract against pathogenic bacteria of fish and shrimp D N Lestari1, A Massinai1 and A Haris1 1Faculty of Marine Science and Fisheries, Hasanuddin University, Makassar, Indonesia Abstract Polycarapa aurata is one of the ascidians from the subphylum Urochordata that produces secondary metabolites that can be used as antibacterial agents. This study aimed to examine the potential of Polycarpa aurata extract as an antibacterial against pathogenic bacteria of aquatic organisms, namely Vibrio parahaemolyticus and Vibrio harveyi. This research was conducted from February to July 2020, including sample collection and preparation, extraction, and antibacterial testing. Samples of Polycarpa aurata were obtained from the reef flat waters of Barranglompo Island, Makassar City. Sample preparation was carried out to produce a dry sample which was then continued in the extraction process by the multilevel maceration method (1:3). The solvents used were n-hexane (nonpolar) and ethanol (polar), which yielded a 1.6% yield of n-hexane extract and 2.21% ethanol extract, respectively. The antibacterial activity test was carried out using the disc diffusion method (Kirby Bauer Test) and measured the average clear zone against the two test bacteria. The n-hexane extract of Polycarpa aurata showed antibacterial potential against Vibrio parahaemolyticus 0.73 mm and Vibrio harveyi 1.24 mm with a weak category. Meanwhile, the ethanol extract of Polycarpa aurata showed weak antibacterial activity against Vibrio parahaemolyticus 4.21 mm and moderate category against Vibrio harveyi 6.69 mm. Keywords: antibacterial, Polycarpa aurata, Vibio parahaemolyticus, Vibrio harveyi Share Link | Plain Format | Corresponding Author (Dwi Nining Lestari)

135	Marine and Fisheries Biotechnology (International)	ABS-187
Optimization of the Utilization from Mangrove Fruit Rhizophora mucronata Lamk. Extract as a Preparation in Making Hand Sanitizer RD Ayuningtyas1*, A Munandar1 and D Surilayani1 1Department of Fishery Science, Faculty of Agriculture, University of Sultan Ageng Tirtayasa, Serang, Indonesia *Corresponding author: 4443170042[at]untirta.ac.id Abstract Antibacterial is a substance that can inhibit or kill the growth of bacteria that cause infection. Staphylococcus aureus is one of the most common infections caused by bacteria. In this research, the ripe mangrove fruit Rhizophora mucronata Lamk. was used to make antibacterial. Ripe mangrove fruit contains biochemical compounds such as flavonoids, tannins, hydroquinone, triterpenoids and saponins. These compounds can be used as antibacterial. This research aims to determine the formulation and evaluation of hand sanitizer preparation from mangrove fruit methanol extract against several test parameters, such as organoleptic test, pH test, viscosity test and antibacterial test. The treatments were made in four concentrations (0%, 0,25%, 0,5%, and 0,75%) and two replications. The bacteria used for antibacterial testing is a Staphylococcus aureus by using the method of diffusion wells. The result showed that the composition of methanol extract from mangrove fruit (Rhizophora mucronata Lamk.) 0,25% is the best treatment of hand sanitizer. This hand sanitizer has antibacterial activity against Staphylococcus aureus bacteria inhibition zone of (8.00 mm), has viscosity (2287.3 Cps) and pH (4.64). In addition, hedonic test also showed that hand sanitizer has color (3.37), texture (3.90) and aroma (3.37). Keywords: antibacerial, hand sanitizer, Rhizophora mucronata Lamk. Share Link | Plain Format | Corresponding Author (Risha Dara Ayuningtyas)

136	Marine and Fisheries Biotechnology (International)	ABS-200
Effects of Physical Treatments on Size Particle and Nutritional Properties of Bone Powder from Pangasius sp. Jasmadi, AR Sefrienda, B Kumayanjati, DED Setyono, H Herdian and H Novianty* Research Centre for Food Technology and Processing- National Research and Innovation Agency (BRIN) hildanovianty2012[at]gmail.com* Abstract Fishbone (FB) is a nutritionally dense byproduct of fisheries processing, which is a good source of calcium and other elements. This study aimed to determine the effect of sonication and water bath treatments on the size and nutritional value of Pangasius bone powder. Pulverized FB was soaked in 40% lime solution (pH 2) under different conditions, in sonicator and water bath in 30 min. and 60 min. at 60 C, and then powdered. The whiteness index (WI), pH, particle and distribution size, proximate and mineral contents of FB powder were investigated. Findings revealed that sonication treatments were 89.09-89.17 for WI, 6.99-7.14 for pH, 860.15-1029.3 nm for particle size, 3.66-3.95 % for fat, 14.62-16.10 % for protein, 50.48-50.91% for Ca, 14.77-15.08 % for P. While water bath treatments were 88.91-88.93 for WI, 7.36-7.43 for pH, 734.5-959 nm for particle size, 3.22-3325 % for fat, 14.78-15.23 % for protein, 50.97-50.95 % for Ca, and 14.97-14.99 % for P. Both showed a mono-modal size distribution and trace amounts of other minerals in all treatments. In conclusion, a 60-minute water bath treatment is a promising method for reducing the particle size of FB powder without significantly altering its nutritional composition. Keywords: fishbone, Pangasius, sonication, water bath, particle size, nutrition Share Link | Plain Format | Corresponding Author (Hilda Novianty)

137	Marine and Fisheries Biotechnology (International)	ABS-220
Natural Antibacterial from Noni Fruit Extract (Morinda citrifolia) against Fish Pathogenic Bacteria Elmi Nurhaidah Zainuddin (a*), Marlina Achmad (a), Huyyirnah (a), Fajriati Ainun (a) a) Marine Science and Fisheries Faculty, Hasanuddin University. Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar 90245. *elmi18id[at]gmail.com Abstract Freshwater fish has a very large potential to be developed in the field of aquaculture in Indonesia. Freshwater fish commodities that have potential to be cultivated include, catfish, tilapia, carp, ornamental fish, and crayfish. Increasing demand for these commodities in Indonesia, both consumption and non-consumption purposes (ornamental fish) is also caused increasing in intensification to meet production needs. This intensification can also have an impact on increasing the risk of pests and diseases of fish that have the potential to damage the sustainability of fishery biological resources. In this study, the types of pathogenic bacteria used are those that generally attack freshwater fish and seawater fish, such as Aeromonas hydrophila, Flavobacterium sp., Serratia sp., Pseudomonas stutzeri, Bacillus flexus and Bacillus cereus. The use of natural antibiotics in cultivation is highly recommended as a substitute for synthetic commercial antibiotics which have side effects. This study aims to see the potential of noni fruit extract (Morinda citrifolia), as a candidate for natural antibacterial drugs. Noni fruit extraction was carried out by kinetic maceration method in stages with solvents of different polarity (n-hexane, chloroform and methanol) and the highest yield was found in methanol extract of 3.40%. The antibacterial activity test was carried out using the agar diffusion method and the results showed that all extracts of the noni fruit (Morinda citrifolia) showed a significant effect on the test pathogenic bacteria (p<0.05). Meanwhile, the results of the Tukey test showed the highest activity was led by n-hexane extract of noni fruit against fish pathogenic bacteria Flavobacterium sp. and Bacillus cereus with an inhibition zone diameter of 14.33 mm, respectively. From this study, it can be concluded that noni fruit extract (Morinda citrifolia) has the potential as a natural antibacterial against fish pathogenic bacteria. Keywords: Morinda citrifolia extracts- Natural antibacterial- Fish pathogenic bacteria Share Link | Plain Format | Corresponding Author (Dr.rer.nat. Elmi Nurhaidah Zainuddin DES)

138	Marine and Fisheries Biotechnology (International)	ABS-239
Antioxidant and antibacterial activity of Holothuria atra from Lombok Island, Indonesia Yanuariska Putra1, Fawzan Bhakti Soffa1, Muhammad Firdaus1, Ratih Pangestuti2, Evi Amelia Siahaan1* 1 Head of Research Center for Marine and Land Bioindustry, National Research and Innovation Agency, Jl. Raya Senggigi, Teluk Kodek, North Lombok, Indonesia 83352 *eviamelia.siahaan[at]gmail.com 2 Head of Research Center for Food Process and Technology, Jl. Jogja-Wonosari, Playen, Yogyakarta, Indonesia 55861 Abstract Recently, much attention has been paid on the anti-tumor activity, anti-cholesterolemic activity and antioxidant activity of seaweed constituents. Consequently, antioxidant activity is intensively focused due to the currently growing demand from the pharmaceutical industry where there is interest in anti-aging and anti-carcinogenic natural bioactive compounds, which possess health bene&#64257-ts. Black sea cucumber, Holothuria atra, is the potential source of terpene glycoside with high antioxidant activity. In this study, the antioxidant, antibacterial activity and fatty acids of H. atra was evaluated. H. atra was collected from Lombok. Fatty acid composition of fresh H. atra was determinated by gas chromatographic quantification. Three different solvents (ethanol, methanol and ethanol mixed with acetone) were used to extract the phenolic content of body wall and viscera of H. atra. Keywords: Antioxidant- antibacterial- fatty acid- Holothuria atra Share Link | Plain Format | Corresponding Author (Evi Amelia Siahaan)

139	Marine and Fisheries Biotechnology (International)	ABS-242
Molecular docking study of Halymenia durvillei extract (Rhodophyta) against VP26 Protein of White Spot Syndrome Virus Asmi Citra Malina A.R. Tassakka*(a), Israini Wiyulanda Iskandar(b) , Kasmiati (a) , Jamaluddin Fitrah Alam (a), and Widyastuti Umar (a) (a)Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia (b)Center for Excellence in Science and Technology of the Development and Utilization of Seaweed (PUI-P2RL) Abstract Virus infections have hampered the development of shrimp aquaculture, putting a significant financial strain on the business. White spot disease (WSD) has caused significant mortality in farmed shrimp in many regions. The envelope proteins VP26 are essential in the infection process and interactions with host cells. In this recent study, the bioactive compounds from Halymenia durvillei algae have been investigated by molecular docking against VP26 protein from White Spot Syndrome Virus in Shrimp. The 37 natural compounds of Halymenia durvillei algae identified by GC/MS analysis have shown that E, E, Z-1,3,12-Nonadecatriene-5,14-diol have the highest fitness score in the GOLD calculation in molecular docking with 21.9. The docking analysis would be used to identify a drug-like molecule as an immunostimulant, which would then be optimized for a potential treatment for shrimp aquaculture. This is thought to be the role of the inhibitor, which prevents the viral protein from attaching to the receptor and therefore prevents the WSSV infection. Keywords: GC-MS, Halymenia durvillei, natural compounds, Shrimp aquaculture, WSSV Share Link | Plain Format | Corresponding Author (Israini Wiyulanda Iskandar)

140	Marine and Fisheries Geographic Information System (International)	ABS-63
Mapping indicative location for beje pond as fish source in tropical peat swamp using Landsat 8 OLI-TIRS Y Aguswan, S Gumiri, RM Sukarna, I Permana University of Palangka Raya Abstract Tropical peatlands in Indonesia are severely degraded due to unsustainable logging, conversion to plantations, poor drainage, and repeated fires. Traditional communities in Central Kalimantan used to build beje ponds for fishing and paludiculture farming system in deep peatlands area that are frequently flooded. However, hardly reached spot in peat swamp makes areas suitable for beje difficult to find. This study aims to map the location suitable for beje using Normalized Difference Water Index (NDWI) from Landsat 8 OLI Imagery. Canopy results from the NDWI were used as a reference for field investigations at nine sites from each NDWI class. The classification test results show that NDWI is able to distinguish dry and wet areas in tropical peatlands, with a manufacturer accuracy of 83.3 per cent and user accuracy of 84.9 per cent. As a result, the area suitable for beje ponds development is about 9,616 ha, or 6.2 per cent of the total area studied. Keywords: Remote sensing, Landsat 8 OLI, NDWI, beje, peatswamp, livelihoods Share Link | Plain Format | Corresponding Author (Yusuf Aguswan)

141	Marine and Fisheries Geographic Information System (International)	ABS-189
Generating tsunami hazard map with various relief (Land) in Indonesian coastal cities by remote sensing perspective: A preliminary study Martiwi Diah Setiawati (a*), Ranie Dwi Anugrah (b), Marindah Yulia Iswari (c), Abu Bakar Sambah (d), Abd. Rahman As-syakur (e), Ali Yansyah Abdurrahim (f), Kasih Anggraini (a) (a*) Research Center for Oceanography, National Research and Innovation Agency (BRIN), Jakarta, Indonesia (b) Ministry of Agrarian Affairs and Spatial Planning / National Land Agency, Jakarta, Indonesia (c) Research Center for Hydrodynamics Technology, National Research and Innovation Agency (BRIN),Surabaya, Indonesia (d) Faculty of Fisheries and Marine science, Brawijaya University, Malang, Indonesia (e) Faculty of Fisheries and Marine science, Udayana University, Denpasar, Indonesia (f) Research Center for Population, National Research and Innovation Agency (BRIN), Jakarta, Indonesia Abstract Tsunami-prone areas on the western and southern coasts of Sumatra, Java, and Bali are home to more than a hundred million Indonesians. As preliminary research, the main goals of this paper are to present a hazard assessment methodology that identifies areas of high tsunami hazard and the spatial distribution pattern of inundated areas and its energy transfer to the inland with various relief (land) in coastal cities. The methods established here are based on a geographic information system (GIS) approach by combining the numerical modeling, wave height, and remote sensing data. We also examined the variations of spatial hazard distribution with a medium spatial resolution (30m) among the pilot sites studies. The result stated that medium spatial resolution has limitations to model the inland tsunami inundation and its energy in the plain area. Still, it can model in the dynamic elevation variation. Under the 10 m wave height scenario, the highest mean of inundation depth among four pilot sites is in Kuta (5.9 m with an energy wave (Ewave) of 38.34 MJ ), followed by Banyuwangi (5.5m with Ewave of 33.32 MJ), Pacitan (5m with the Ewave of 27.53 MJ) and Sibolga (3.9 m with the Ewave of 16.75MJ), respectively. Keywords: Tsunami hazard map, inland energy wave, inland inundation, remote sensing, GIS Share Link | Plain Format | Corresponding Author (Martiwi Diah Setiawati)

142	Marine and Fisheries Geographic Information System (International)	ABS-193
Spatial Analysis of Blue Swimming Crab Fishing Areas in The Banten Bay Muhammad Fakhri Alwi, Ririn Irnawati, Hery Sutrawan Nurdin Sultan Ageng Tirtayasa University Abstract Blue swimming crab (BSC, Portunus pelagicus) is commonly found throughout Indonesia, but most crab fishing effort is concentrated in North Java, Lampung, and Sulawesi, including Banten Bay. Catching BSC takes place throughout the year in Banten Bay. BSC fishers in the Banten Bay carry out fishing operations using a bottom gillnet called crab gillnet. So far, in determining the location of fishing areas, fishers generally still use traditional methods based on the fishers^ abilities or those obtained from generation to generation. This study aimed to analyze the spatial distribution and characteristics of the crab fishing area in Banten Bay and identify the catch from the crab gillnet. The research method used is a survey with experimental fishing. This study explores crab fishing areas and their characteristics by measuring depth and identifying the bottom substrate, temperature, salinity, and water brightness. Using the purposive sampling method, a sampling of research locations in 21 trips. The result shows that the spatial location distribution of the BSC fishing ground is around Tarahan Island, Lima Island, Kubur Island, and Burung Island. The fishing ground characteristics include temperature range from 25-28.5oC, depth 3.3-12.5 m, salinity 25-30 ppt, brightness values 100-135, and mud bottom substrate. The catch composition included BSC (Portunus sp.) 64,5%, eel tailed catfish (Plofosus sp.) 0,1%, mud crab (Scylla sp.) 17,4%, shrimp (Penaeus sp.) 13,6%, grouper (Epinephelus coioides) 0,2%, squid (Loligo) 1,1% and octopus (Octopoda) 0,1%. Kubur island (3rd trip) is a potential location for fishing BSC in Banten Bay water. Keywords: blue swimming crab, fishing ground, Banten Bay Share Link | Plain Format | Corresponding Author (Muhammad Fakhri Alwi)

143	Marine and Fisheries Geographic Information System (International)	ABS-217
Spectral and Spatial properties of Seaweed Farming at South Sulawesi Muhammad Banda Selamat (a*), Rustam (b), Muhammad Farid Samawi (a) a) Marine Sciences, Universitas Hasanuddin Jl. P. Kemerdekaan km 10, Makassar 90245, Indonesia * mbandaselamat[at]unhas.ac.id b) Aquaculture, Universitas Hasanuddin Abstract Indonesia^s total seaweed production in 2020 was estimated to reach 9.6 million tons, and 36% of production comes from South Sulawesi. The Seaweed farming areas in South Sulawesi are commonly found over the East, South, and West coasts covering 17 districts. This study aims to examine the differences in the spectral characteristics of seaweed farming on the west and east coasts of South Sulawesi, based on the Sentinel 2 satellite optical sensor^s spectral bands. It examines the spatial character of seaweed farming in the two regions as well. There were two Sentinel 2 imagery used, namely T50MQV as the representation of the West coast, and T51MTR which represents the East coast. Both images were atmospherically corrected using the DOS method to obtain reflectance values. Resampling was done to all bands to get a uniform spatial resolution at 10 meters. The TSS (Total Suspended Solid) algorithm was applied to obtain a TSS image. The statistic shows that both two areas were different spectrally. On the West coast, TSS inside of seaweed farming units commonly does not differ from the surrounding environment. While on the East coast, the TSS in the seaweed farming unit is generally lower than its surrounding. Overall, the TSS level on the West coast is lower than on the East coast. Seaweed farming units on the West tend to be closer to the coast and have a smaller size area compared to those on the East coast. Keywords: seaweed farming- Sentinel 2- remote sensing- TSS Share Link | Plain Format | Corresponding Author (Muhammad Banda Selamat)

144	Marine and Fisheries Geographic Information System (International)	ABS-224
Mangrove health distribution on forest classes in the eastern waters of Biak Island, Papua I Wayan Eka Dharmawan, Dietriech G. Bengen, I Wayan Nurjaya, Rastina, Yaya Ihya Ulumuddin, Doni Nurdiansah, Jeverson Renyaan Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University, Bogor, Indonesia Research Center for Oceanography, National Research and Innovation Agency, Jalan Pasir Putih 1. No 1. Ancol Timur, Jakarta Utara Abstract Mangrove structure influenced by geomorphological and environmental parameters gradient forms specific group dominated zones. A study of mangrove zonation in small islands was conducted along the eastern waters of the Biak, Papua. This study aimed to investigate mangroves zones distribution and their areas. Another objective of this study was to spatially determine the mangrove health index along the zones in seven mangrove sites, i.e. Ruar-Yenusi, Tanjung Barari, Owi, Auki, Wundi, Pai, and Padaidori island. The random forest technique was applied in the Google Earth Engine platform to classify mangrove zones. On the other hand, MHI distribution was estimated by a spatial model combining several vegetation indices. Classification analysis resulted in high accuracy by 93% of overall accuracy and 0.91 of kappa coefficient. The Rhizophora-dominated forest was dominant in Tanjung Barari and Padaidori island, while the Bruguiera zone had a majority distribution in Pai and Auki islands. Sonneratia zone has significant coverage in Owi and Wundi islands. Those three zones were found in a similar area in Yenusi-Ruar mangrove. In terms of MHI distribution, Rhizophora and Bruguiera forests were dominantly in an excellent category which indicated that mangroves on the Biak^s eastern coast were in a pristine condition. Keywords: Mangrove- Forest Zone- Random Forest Classification- Mangrove Health Index- Share Link | Plain Format | Corresponding Author (I Wayan Eka Dharmawan)

145	Marine and Fisheries Geographic Information System (International)	ABS-233
The Issue of Microplastics (MPs) in Coastal Areas of Indonesia: Bibliometric Network Analysis Yenni Putri Sari (a), Mukti Dono Wilopo (a), Siti Ayu Ulfadillah (b), Elsa Vandana (a), Rindi Puja Listari (a) a. Marine Science Study Program, Agriculture Faculty, University of Bengkulu (UNIB), Indonesia b. Institute of Food Safety and Risk Management, National Taiwan Ocean University (NTOU), Taiwan Abstract Microplastics (MPs) are a concern in both natural and artificial fisheries. Microplastics research is currently one of the fastest-growing fields of study in the scientific community. This study describes and evaluates the results of microplastic research conducted in Indonesia between 2010 and 2022 and published in scholarly journals. There will be a search of the Scopus database for scholarly papers comprising more than 100 articles having the terms microplastic, sediment, waterways, and environment. Using the program of VOSviewer, a bibliometric study will be conducted. The descriptive research will focus on author and institution production, while the bibliometric analysis will focus on author and institution collaboration and cluster analysis. The findings of the analysis indicate that microplastics-related research topics have drawn substantial interest in Indonesia, particularly over the past five years (2016-2022), as indicated by the steady increase in the number of publications. In the coming years, this rising tendency is expected to persist. The results of a bibliometric analysis of keywords indicate that the topic of microplastic research in Indonesia can be categorized into three clusters and highlighted as relevant study areas. Keywords: Microplastic, bibliometric, Indonesia, and VOSViewer Share Link | Plain Format | Corresponding Author (yenni putri sari)

146	Marine and Fisheries Geographic Information System (International)	ABS-235
Identification of potential areas for upwelling based on characteristics of Eddie event in the Bone Gulf Rachmat Hidayat*, Mukti Zainuddin, Safruddin, Eko Sri Wiyono Faculty of Marine Science and Fisheries, Hasanuddin University, South Sulawesi, Indonesia Department of Marine Science and Technology, IPB University, Bogor, Indonesia Abstract The Bone Gulf is one of the waters with massive potential for catching pelagic fish. The potential for catching fish is associated with oceanographic events in the waters. This study aims to see the potential for upwelling in the eddy occurrence areas. The spatial analysis method will be used to see the potential for upwelling in the area of the eddy occurrence and will be associated with sea surface temperature parameters and chlorophyll-a as a fertility variable. The results of this study indicate that eddy events occur every month in the Bone Gulf waters. In addition, it can be proven that every eddy occurrence is not a guarantee for the emergence of upwelling areas simultaneously. However, several eddy events with high current velocities were seen to cause a decrease in temperature in the middle of the eddy and increase the chlorophyll-a concentration in that area. In addition, the suitability of this event is also shown from the time of upwelling, which occurs several months before the peak month of fishing in the Bone Gulf. This research is expected to be the basis for the sustainable development of fisheries. Keywords: Eddies- SSC- SST- Sustainable fisheries development, Upwelling Share Link | Plain Format | Corresponding Author (Rachmat Hidayat)

147	Marine and Fisheries Socio - Economics and Culture (International)	ABS-268
Analysis of Law Enforcement on Marine Debris in Indonesia Eka Merdekawati Djafar(a), Tri Fenny Widayanti (b*), M. Zulfan Hakim(a), Syarif Saddam Rivanie (c) a) Constitutional Law Department, Faculty of Law, Hasanuddin University b) International Law Department, Faculty of Law, Hasanuddin University email : trifenny[at]unhas.ac.id c) Criminal Law Department, Faculty of Law, Hasanuddin University Abstract Marine debris one of the source of marine pollution that will damage marine ecosystems and be harmful to human health. To deal with marine debris, the government has formed a national action plan for marine debris 2018-2025 as the implementation of Presidential Regulation No. 83 of 2018 concerning the handling of marine debris. Law enforcement is one of the strategies in the action plan, but in practice, law enforcement cannot implemented effectively. The absence of sanctions given to the garbage throwers and the absence of socialization of the rules regarding marine debris make there no deterrent effect and therefore increase the amount of marine debris in Indonesia. This study uses a normative juridical approach with analytical descriptive research to describe and analyze the use of international legal instruments nationally. The results of this study are that strict rules must be made, especially the imposition of sanctions on the perpetrators of marine waste disposal so that it will have a deterrent effect, sanctions can be in the form of fines or criminal sanctions. The government also must socialize to the public regarding the provision of these sanctions, so that there is good cooperation. between the government, and the community Keywords: Marine Debris, Law enforcement, Sanction Share Link | Plain Format | Corresponding Author (Tri Fenny Widayanti)

148	Marine and Fisheries Socio - Economics and Culture (International)	ABS-273
The role of the fisherwomen in supporting the fishery business and improving the family economy in the Banten Bay Coast Ririn Irnawati, Dini Surilayani, Ana Susi Mulyani (1) Department of Fisheries Science, University of Sultan Ageng Tirtayasa (2) Centre of Excellence for food security (local food innovation) (3) Department of Management, University of Sultan Ageng Tirtayasa Abstract Fishers^ women act as housewives and do productive activities to increase income in the family. The perspective of fishers^ women on supporting the fisheries business and strengthening the economy of fishers^ households needs to be studied to realize the critical role and contribution of fishers^ women to improve the family economy. This study aims to identify and analyze the role of women in fishing activity and the economy of fishers^ households. Data were collected through in-depth interviews and observation in the Karangmulya sub-village, Banten Village of Serang City. Data were analyzed using the descriptive method. The results show that fishers^ women have domestic, social, and productive work roles. Domestic roles are cooking, cleaning and tidying the house, taking care of children, and helping husbands in fishing activities. Social role actively in religious activities. The productive roles of fisherwomen include grocery stores, fuel sellers, cooks, net repair workers, and net-making workers. The part in fisheries activity is preparing supplies and fuel, handling supplies and equipment, repairing fishing gear, and sorting fish. All the roles of women fishers are very supportive in maintaining and improving the economic conditions of fishers^ families. Keywords: family economy, fisherwomen, fishery activity, productive work, role Share Link | Plain Format | Corresponding Author (Ririn Irnawati)

149	Marine and Fisheries Socio - Economics and Culture (International)	ABS-19
Fresh fish trade at Karangantu fishing port, Banten, Indonesia Thomas Nugroho (a*), Rama Aldyansyah (a), Sulistiono (b) a) Department of Fisheries Resources Utilization, Faculty of Fisheries and Marine Sciences, IPB University (Bogor Agricultural University), Jl. Agatis Darmaga Bogor, Bogor 16680, Indonesia * thomasnu[at]apps.ipb.ac.id b) Department of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, IPB University (Bogor Agricultural University), Jl. Agatis Darmaga Bogor, Bogor 16680, Indonesia Abstract Fishery products from fishing business at the Karangantu Fishing Port (PPN Karangantu) provide great benefits, namely being able to move the community^s economy, including through the sale of fresh fish. The fish market at PPN Karangantu is is classified as crowded by the people. Besides being close to the fish-landing site, this fish market is also a marine tourism area that is visited by the people from in and outside Serang City. This study aims to describe the pattern of fish trade and to calculate the transaction value of buying and selling fresh fish in market at the Karangantu Fishing Port. The research was conducted in September-November 2021. Data were collected and analyzed descriptively. The results of the study show that the trade value of a fish collector reaches IDR 124.595.570 per month with an average sales value of fish per day of IDR 4.153.186. Meanwhile, the trade value of a small trader in market reached IDR 56.093.000 per month with an average value of IDR 2.671.095 per day. Keywords: Fish trade- fishing port- Karangantu Share Link | Plain Format | Corresponding Author (Thomas Nugroho)

150	Marine and Fisheries Socio - Economics and Culture (International)	ABS-285
OPERATING SYSTEM AND MARKETING OF FLYING FISH EGGS IN TAKALAR DISTRICT, SOUTH SULAWESI, INDONESIA Nadir, M. Saleh S. Ali, Andi Adri Arief, Letty Fudjaja, Rahmadanih Graduate School, Hasanuddin University, Makassar, Indonesia Abstract Flying fish catching business is a source of livelihood for most fishermen in Takalar Regency. This fishery activity includes about 20 thousand fishermen, courtiers, papalele, daily workers and intermediaries along the Galesong coast of Takalar Regency. This study aims to analyze the fishing operation system, and marketing of flying fish eggs. This research uses a case study approach with qualitative descriptive analysis. The research was conducted in the coastal area of the patorani fishing community in Galesong District, Takalar Regency, South Sulawesi. The research data analysis used qualitative data analysis, by collecting data systematically, doing data reduction, then the data obtained were selected, focused, simplified and abstracted according to the field notes obtained. Then the presentation of the data is classified and the last is drawing conclusions or interpretations. The results showed that fishermen started their activities in March to October. Starting with preparing all the necessities that will be used in catching operation, this preparation includes equipment, boat repairs or renovations, making flying fish roe fishing gear made of coconut leaves and bamboo called pakkaja or balla-balla. In addition, prepare all needs while in the ocean later, such as the need for fuel oil and daily supplies. Fishermen are doing catching around the sea of the Pangkajene Islands and will return to the coast to give or sell their catch of flying fish eggs to investors or traders, while patorani fishermen operating around the waters of Fak-Fak West Papua because the long distance does not make it easy for fishermen to return home so that fishermen deviate from their results. If the catch is large, it will be sent to papalele in Makassar. Marketing of flying fish eggs is done by fishermen giving their catch to papalele (owners of capital), then papalele sells to wholesalers and before being sold, wholesalers process them first for export. Keywords: Operation and Marketing, Flying Fish Egg, Pakkaja, Papalele, Takalar District Share Link | Plain Format | Corresponding Author (Nadir Nadir)

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