Prevention of Vibriosis using wholecell microalgae in tiger shrimp hatcheries
Nurbaya1, Ince Ayu K.Kadriah*1, Muharijadi Atmomarsono1, Muliani1 and Hilal Anshary2

1Nasional Research and Innovation Agency
2Faculty of Fisheries and Marine Science, Hasanuddin University

Abstract

Vibriosis is the main cause of mass mortality in a tiger shrimp hatchery.To see whether whole-cell microalgae Porphyridiumsp and Porphyridiumaerugineumcan be used for preventingvibriosis in tiger shrimp larvae, this study uses a completely randomized design with three treatments and three replications each was carried out in the RIBAFE hatchery in Awarange, Barru. Three treatments tested here were A) Control (without additional microalgae), B)Porphyridiumaerugineum, and C)Porphyridium sp. These two microalgae with a stocking density of about105cells/mLwere administered in the rearing tank at the shrimp stage of Zoea1, Mysis1, PL2, PL6, and PL10.The density of microalgae was observed every day from Zoea-2 to PL10, whilethe density of bacteria was measured at the above stages. At the end of the fry rearing period (PL12), a challenge test was carried out using the bacteria Vibrioparahaemolyticus with a density of 106 CFU/mL.The results showed that the administration of P. aerugineum could suppress the population of Vibrio bacteria and the ratio of Vibrio bacteria to common bacteria.However, the best survival rate of tiger shrimp postlarvaewas obtained by treatment C(Porphyridiumsp).

Keywords: Mikroalgae, Porphyridium sp, Porphyridium aerugineum, Vibriosis

Topic: Marine and Fisheries Biotechnology (International)