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181	Penangkapan Ikan Berkelanjutan (National)	ABS-100
Keanekaragaman Jenis dan Produktivitas Penangkapan di Ekosistem Padang Lamun pada Waktu Siang dan Malam Hari Menggunakan Alat Tangkap Minnow Trap di Desa Parak, Pulau Selayar Nursalam Saputra(1*), Andi Assir Marimba(1), Alfa F.P. Nelwan(1) (1)Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin, Makassar. *nursalamsptra[at]gmail.com Abstract Penelitian ini bertempat di perairan padang lamun Desa Parak, Pulau Selayar pada bulan Oktober hingga Desember 2021. Tujuan dari penelitian ini (1) mengetahui komposisi jenis hasil tangkapan siang dan malam (2) mengetahui frekuensi kemuculan siang dan malam (3) melihat nilai indeks keanekaragaman spesies siang dan malam (4) mengetahui produktivitas hasil tangkapan siang dan malam. Metode penelitian yang digunakan yaitu studi kasus pada pengoperasian 6 buah minnow trap secara langsung di lapangan selama 15 trip. Hasil penelitian menujukkan total 148 ekor tertangkap, dimana 33 ekor tertangkap pada waktu siang dan 115 tertangkap pada waktu malam. Jumlah hasil tangkapan pada malam hari jauh lebih tinggi dibandingkan siang hari. Total spesies yang tertangkap sebanyak 10 family yang terdiri atas 13 spesies. Hasil tangkapan terdiri atas 3 kelompok yaitu gastropoda, ikan dan krustasea. Komposisi jenis spesies yang tertangkap pada malam hari lebih tinggi dibandingkan siang hari. Proporsi kemunculan spesies per trip di dominasi lebih tinggi pada malam hari. Nilai indeks keanekaragaman jenis menujukkan waktu siang dan malam termasuk kategori sedang. Indeks keanekaragaman siang lebih tinggi dibandingkan malam hari. Produktivitas penangkapan berdasarkan waktu menujukkan pada malam hari lebih tinggi dibandingkan siang hari. Keywords: Minnow trap, siang dan malam, padang lamun, Pulau Selayar Share Link | Plain Format | Corresponding Author (Nursalam Saputra)

182	Penangkapan Ikan Berkelanjutan (National)	ABS-210
The Sustainability Levels Of Purse Seine In Bontobahari Waters Of Bulukumba Regency Muh. Aldhy Hatmar (a*), Achmar Mallawa (a), Muhammad Kurnia (a) a) Fisheries Resource Utilization Study Program, Faculty of Marine and Fishery Sciences, Hasanuddin University. Abstract This study aims to determine the level of sustainability of purse seines operated in FADs plus lights and non FADs in the waters of Bontobahari, Bulukumba Regency. This research was conducted in June and November 2020 in Bontobahari District, Bulukumba Regency, South Sulawesi. The research method used is the case study method. The data needed in this study consisted of primary and secondary data. Primary data includes biological data of the catch, technical fishing gear and socio-economics of the purse seine business. While secondary data was taken from interviews and the Department of Marine Affairs and Fisheries of Bulukumba Regency. The sustainability of purse seine was analyzed using 14 criteria according to the Mallawa modified Arimoto method. The results showed that purse seine without FADs had moderate level of sustainability with a value of 76.8%. Meanwhile, purse seine with FADs plus lights have a low level of environmental friendliness or less environmentally friendly with a value of 63.75%. The low level of sustainability of fishing technology is caused by the dominance of small size fish and the low size of fish suitable for catching, investment value, income level, and fuel and the capture of protected marine biota. Keywords: Purse seine, FADs, non-FADs, sustainability Share Link | Plain Format | Corresponding Author (Muh Aldhy Hatmar)

183	Pencemaran dan Ekotoksikologi Laut (National)	ABS-118
Mengidentifikasi Adanya Kandungan Mikroplastik Pada Makroalga Jenis Codium fragile di Kabupaten Takalar Sri Dawana (a*) Inayah Yasir (b) Shinta werorilangi (b) Farid Samawi (b) Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin Jl. Perintis Kemerdekaan No. KM. 10, Tamalanrea Indah, Kec.Tamalanrea, Kota Makassar *sridawana[at]gmail.com Abstract Hingga saat ini sampah yang paling mendominasi diperairan laut adalah jenis sampah plastik. Sampah plastik diperairan lambat-laun akan terdegradasi membentuk partikel-partikel kecil yang disebut dengan mikroplastik. Mikroplastik telah banyak ditemukan dilingkungan perairan laut khususnya pada organisme ikan, dan kerang, namun informasi terkait keberadaan mikroplastik pada makroalga masih sangat terbatas padahal beberapa dari jenis makroalga dapat dikonsumsi secara langsung tanpa melalui proses pengolahan. Pada ekosistem perairan makroalga berperan sebagai produsen primer yang dapat berinteraksi secara langsung dengan berbagai biota laut untuk membentuk sebuah rantai makanan. Salah satu makrolga yang banyak dikonsumsi oleh masyarakat adalah jenis Codium fragile. Apabila makrolaga C fragile terkontaminasi dengan mikroplastik dan tertelan oleh organisme lain, mikroplastik dapat berpindah serta terakumulasi antar organisme di tingkat trofik yang berbeda dalam jaring makanan. Jika hal ini terus berlanjut dapat menimbulkan dampak yang lebih serius karena berimplikasi pada manusia yang menduduki posisi puncak tingkat tropik pada hampir semua rantai makanan dalam ekosistem. Penelitian ini bertujuan untuk membuktikan keberadaan mikroplastik pada makroalga jenis C.fragile yang sering dikonsumsi oleh masyarakat di perairan Kabupaten Takalar, serta mengidentifikasi karakteristik mikroplastik meliputi warna, ukuran dan bentuk. Makroalga yang akan diamati di ekstraksi terlebih dahulu menggunakan Kalium Hidroksida (KOH) 10% dan pengamatan karakteristik mikroplastik (MP) dilakukan menggunakan bantuan mikroskop. Keywords: Mikroplastik (MP), Makroalga, Kabupaten Takalar Share Link | Plain Format | Corresponding Author (SRI Dawana)

184	Pencemaran dan Ekotoksikologi Laut (National)	ABS-120
Komposisi dan Kelimpahan Mikroplastik Pada Caulerpa racemosa di Kabupaten Takalar Provinsi Sulawesi Selatan King Abdul Azis(a), Inayah Yasir(b), Shinta Werorilangi(b), Farid Samawi(b*) a) Mahasiswa Program Studi Ilmu Kelautan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin. b) Fakultas Ilmu Kelautan dan Perikanan, Universitas Hasanuddin. Jl. Perintis Kemerdekaan No. KM. 10, Tamalanrea Indah, Kec.Tamalanrea, Kota Makassar. *farids.unhas[at]gmail.com Abstract Plastik telah menjadi isu global terkait dengan penggunaannya dalam jumlah yang sangat besar di berbagai jenis produk. Penggunaan plastik yang berlebih dan tidak ditangani dengan baik dapat menyebabkan terjadinya pencemaran lingkungan baik di daratan maupun di lautan. Keberadaan plastik dilautan tidak hanya ditemukan dalam potongan besar melainkan juga dalam bentuk partikel kecil yang dikenal dengan sebutan mikroplastik. Keberadaan mikroplastik dilautan tidak hanya mencemari perairan namun juga berdampak biota laut salah satunya makroalga. Dalam ekosistem laut makroalga memiliki kemampuan untuk mengurangi kecepatan arus sehingga berpotensi mefasilitasi pengendapan mikroplastik. Sehingga makroalga diprediksi dapat memerangkap mikroplastik pada struktur morfologi luar tubunya (Ng et al., 2022). Adanya potensi mikroplastik untuk menempel pada makroalga dapat membahayakan organisme lain tidak terkecuali manusia karena bebebrapa jenisnya dikonsumsi secara langsung oleh manusia. Penelitian ini berfokus untuk melihat karakteristik dan kelimpahan mikroplastik pada makroalga jenis Caulerpa racemosa yang dikonsumsi oleh manusia. Sampel diambil pada dua lokasi yaitu lokasi budidaya dan langsung dari habitatnya di Desa Puntondo, Kabupaten Takalar, Provinsi Sulawesi Selatan. Sampel yang diambil dilakukan ekstraksi mikroplastik dengan menggunakan KOH 10% selama 2-3 minggu. Metode identifikasi yang digunakan adalah metode identifikasi visual (Lusher et al., 2017). Perbedaan kelimpahan rata-rata mikroplastik dalam Caulerpa racemosa antar stasiun dianalisis menggunakan uji t-Test. Pengukuran panjang mikroplastik menggunakan aplikasi image-J. Keywords: Plastik, Mikroplastik, Makroalga, Caulerpa racemossa, Kanupaten Takalar. Share Link | Plain Format | Corresponding Author (King Abdul Azis)

185	Pengabdian kepada Masyarakat Terkait Kemaritiman dan Ketahanan Pangan (National)	ABS-170
PENGEMBANGAN HASIL OLAHAN KERIPIK RUMPUT LAUT DI DESA PITUSUNGGU, KECAMATAN. MA^RANG, KABUPATEN PANGKAJENE KEPULAUAN, SULAWESI SELATAN Abdul Wahid (a*), Benny Audy Jaya Gosari (a), Amilludin (a), A.Wahyuni (b), Endah Murpi Ningrum (c), Andi Muhammad Fauzan (d) (a) Dosen Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin (b) Dosen Fakultas Kesehatan Masyarakat Universitas Hasanuddin (c) Dosen Fakultas Peternakan Universitas Hasanuddin (d) Mahasiswa S1 Fakultas Teknik Universitas Hasanuddin Abstract Desa Pitusunggu, Kecamatan. Ma^rang, Kabupaten Pangkajene Kepulauan, Sulawesi Selatan, merupakan desa yang telah lama membudidayakan rumput laut, dimana hasil-hasil olahan dari rumput laut tersebut dijadikan keripik, selai, dodol, dan sirup. Namun karena kurangnya promosi menyebabkan penjualan hasil olahan rumput laut dari kelompok masyarakat sekitar yang tidak optimal, sehingga hal ini yang menginisiasi kami melaksanakan program pengembangan pemasaran serta improvement pada produk baik dalam segi rasa maupun kemasan. Tujuan pengabdian ini adalah menghasilkan produk rumput laut yang lebih bervariasi dan kekinian sehingga dapat meningkatkan penghasilan bagi para pengelola rumput laut di desa Pitusunggu Kec. Ma^rang. Adapun metode yang digunakana dalam kegiatan pengabdian dengan mengadakan sosialisasi bagi masyarakat sekitar di desa Pitusunggu Kecamatan Ma^rang untuk mengedukasi mereka dengan menggunakan LCD dan layar proyektor. Hasil dari kegiatan yang telah dilaksanakan di desa Pitusunggu pada tanggal 1-20 Desember 2021, berupa produk dengan tampilan kemasan baru dengan tambahan variasi rasa vanila. Diharapkan bisa meningkatkan penjualan setelah menambahkan varian rasa pada produk hasil olahan rumput laut mereka. Meningkatnya penjualan tentunya akan meningkatkan pula pendapatan mereka. Keywords: Keripik, Rumput Laut, Pengembangan, Hasil Olahan Share Link | Plain Format | Corresponding Author (Benny Audy Jaya Gosari)

186	Pengabdian kepada Masyarakat Terkait Kemaritiman dan Ketahanan Pangan (National)	ABS-199
Pelatihan Pengolahan Limbah Tulang Ikan di Desa Lamuru, Kecamatan Tellu Siattinge, Kabupaten Bone Nurul Eka Wijayanti Risa, Aam Azatil Isma Fakultas Sains dan Teknologi, Universitas Muhammadiyah Sinjai Abstract Potensi perikanan tangkap di Desa Lamuru yaitu Tempat Pelelangan Ikan (TPI) merupakan penggerak ekonomi yang dapat meningkatkan kesejahteraan nelayan. Aktivitas pendaratan dan pengolahan ikan yang berada di TPI menghasilkan berbagai jenis limbah yang dapat berpengaruh terhadap lingkungan sekitar. Jika penanganan limbah ikan tidak ditangani secara baik maka dapat mempengaruhi terjadinya pencemaran pada lingkungan TPI dan sekitarnya. Pemanfaatan limbah ikan terutama tulang ikan menjadi bahan baku pakan merupakan hal yang belum pernah dilakukan oleh para pembudidaya, hal tersebut dikarenakan kurangnya pengetahuan dalam bidang pengolahan limbah tulang ikan serta unsur protein yang terkandung didalam limbah tulang ikan. Tujuan pengabdian masyarakat ini adalah untuk mengedukasi masyarakat khusunya para pembudidaya dalam mengolah limbah tulang ikan menjadi tepung ikan. Kegiatan ini dilaksanakan di Desa Lamuru, Kecamatan Tellu Siattinge, Kabupaten Bone. Hasil dari kegiatan yang telah dilaksanakan di Desa Lamuru berupa produk tepung ikan yang bersumber dari limbah ikan. Diharapkan dari pengolahan tersebut dapat mengurangi dampak yang ditimbulkan dari limbah ikan dan dapat mengurangi biaya bahan dalam pembuatan pakan. Keywords: Limbah, Pengolahan, Tulang Ikan Share Link | Plain Format | Corresponding Author (Nurul Eka Wijayanti Risa)

187	Sosial Ekonomi dan Budaya Kelautan dan Perikanan (National)	ABS-101
Analisis Kelayakan Usaha Kelompok Pengolah dan Pemasar Hasil Perikanan (POKHLASAR) di Kabupaten Banyumas Annisa Wening Maharani Putri (a*), Kusdiarti (b), Lies Emmawati (b), Endhay Kusnendar Muljana Kontara (b) (a*) Pusat Riset Perikanan. Gedung BRSDM KP 2. Jl. Pasir Putih 2, Kelurahan Ancol Timur, Kecamatan Pademangan, Jakarta Utara, DKI Jakarta 14430. annisawng[at]gmail.com (b) Pusat Riset Perikanan. Jl. Raya Ragunan No.20, RT.06/RW.07, Kelurahan Jatipadang, Kecamatan Ps.Minggu, Kota Jakarta Selatan, DKI Jakarta 12540. Abstract Penelitian ini bertujuan untuk menganalisis usaha pengolahan ikan air tawar di Kelompok Pengolah dan Pemasar Ikan Poklahsar (Poklahsar) di Kabupaten Banyumas dan Sleman. Tempat dan waktu penelitian dilaksanakan di Kecamatan Kedungbanteng, Kecamatan Kembaran dan Kecamatan Sokaraja, Kabupaten Banyumas pada bulan April sampai dengan Mei 2021. Poklahsar adalah kumpulan pengolah dan/atau pemasar hasil perikanan yang melakukan kegiatan usaha di bidang pengolah dan pemasaran hasil perikanan bersama wadah dalam kelompok. Poklahsar termasuk dalam kelompok usaha skala mikro. Pengambilan sampel dilakukan secara purposive sampling. Metode penelitian menggunakan metode koleksi data melalui wawancara, observasi, kuisioner dengan pendekatan komoditas multispesies. Hasil penelitian menunjukkan usaha pada Poklahsar layak untuk diusahakan berdasarkan beberapa indikator meliputi BEP (Break Even Point) unit, BEP Rupiah, dan B/C ratio. Keywords: Poklahsar, pengolahan ikan, analisa kelayakan usaha, rasio B/C, BEP unit, BEP rupiah Share Link | Plain Format | Corresponding Author (Annisa Wening Maharani Putri)

188	Sosial Ekonomi dan Budaya Kelautan dan Perikanan (National)	ABS-249
Analisis Tingkat Peran Keluarga Nelayan Dalam Menghadapi Perubahan Iklim (Studi Kasus : Nelayan Suku Bajo Desa Terapung, Sulawesi Tenggara) Nurtasya Kharima Ramadhani, Amiluddin, Muh. Dalvi Mustafa, Andi Adri Arief, Benny Audy Jaya Gosari Universitas Hasanuddin Abstract Penelitian ini bertujuan untuk mengetahui peran dari masing-masing anggota keluarga suku bajo dalam mempertahankan kehidupan pada perubahan iklim di Desa Terapung Kecamatan Mawasangka Kabupaten Buton Tengah, Sulawesi Tenggara. Penelitian ini dilaksanakan pada bulan maret 2022 dengan menggunakan metode studi kasus. Pengumpulan data dilakukan melalui kuesioner tertutup atau angket yang disebarkan kepada keluarga nelayan suku bajo yang berada di Desa Terapung. Analisis data pada penelitian ini menggunakan interval rentan kelas dengan kategori 1%-20% yaitu pada kategori tidak efektif, 21%-40% masuk dalam kategori kurang efektif, 41%-60% masuk pada kategori cukup efektif, 61%-80% masuk pada kategori efektif, dan 81%-100% masuk pada kategori sangat efektif. Hasil penelitian menunjukkan bahwa tingkat peran keluarga dalam menghadapi perubahan iklim di nelayan suku bajo yang berada di Desa Terapung berada di interval rentan kelas 61%-80% masuk ke dalam kategori efektif. Peran keluarga tertinggi dilakukan oleh istri nelayan dengan nilai skor 4,00 yaitu istri ikut membantu dalam menyiapkan proses melaut seperti menyiapkan bekal konsumsi dan bahan bakar yang ingin digunakan nelayan pada proses melaut. Salah satu peran penting dalam menghadapi perubahan iklim pada keluarga nelayan suku bajo memperoleh nilai tertinggi yaitu 3, 94 adalah suami tetap melaut saat musim timur dan musim barat untuk menghidupi perekonomian keluarga. Pada musim timur nelayan (suami) fokus pada usaha perikanan tangkap tetapi pada musim barat atau pada saat terjadi perubahan iklim nelayan melakukan pekerjaan lain seperti beralih ke usaha budidaya rumput laut, buruh bangunan, buruh kayum dan buruh harian. Keywords: peran, nelayan, perubahan iklim Share Link | Plain Format | Corresponding Author (Nurtasya Kharima Ramadhani N)

189	Sustainable Aquaculture (International)	ABS-2
THE EFFECT OF RECOMBINANT GROWTH HORMONE ON GROUPER FOR CATFISH PERFORMANCE (CLARIAS BATRACHUS) THROUGH IMMERSION AND rGH-FEED (ORAL BOOSTING) METHODS A. Muh Fajrin Ramadhan F1, Marlina Achmad2*, A Suci Islameini H3, Alimuddin4, Muhammad Fuadi 1Aquaculture Study Program, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254, Makassar, Indonesia Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254 2Fisheries Department, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254, Makassar, Indonesia Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254 3Marine Science Study Program, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254, Makassar, Indonesia 4Aquaculture Study Program, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254, Makassar, Indonesia Jl. Perintis kemerdekaan km 10, Kampus Tamalanrea, Makassar 90254 5Aquaculture Technology & Development PT. Suri Tani Pemuka Banyuwangi, Jl. Gatot Subroto 100, Bulusan, Klatak, Lingkungan Tj., Klatak, Kec. Banyuwangi, Kabupaten Banyuwangi, Jawa Timur 6842, Banyuwangi, East Java Abstract The technology of catfish cultivation, including hatcheries and enlargements, has been mastered by many people due to its relative ease. However, it is difficult for farmers to fulfill market needs due to high demand. An increased growth rate will play an important role in fulfilling this demand for catfish, and can be carried out with genetic engineering technology. Furthermore, easy-to-apply alternative genetic engineering for stimulating catfish growth involves the recombinant growth hormone, commonly called rGH (recombinant Growth Hormone). Therefore, this research aimed to evaluate the effect of rGH administration on catfish growth and survival performance. C. batrachus was treated with rGH through a combination of immersion and rGH feeding (oral boosting) method. The test animals used were catfish with an initial weight of 3.9 - 4.0 grams and an initial length of 7.21-8.11 cm with a maintenance period of 45 days. Furthermore, this research tested 4 treatments with 3 replications each, namely A (control), B (immersion), C (oral boosting feed), and D (immersion + oral boosting feed). The results showed that the administration of rGH gave the best results in the immersion + feed treatment (oral boosting), with a specific growth weight performance value of 4.27 4.31% .day-1, daily growth weight of 0.50-0.54 gram.day-1, daily growth length of 0.198-0.202 cm.day-1, feed conversion of 0.78 -0.82, and survival of 93.03-89.19%. Therefore, the provision of rGH through the immersion + feed method (oral boosting) increases growth performance and high survival rates Keywords: Catfish (Clarias batrachus), recombinant Growth Hormone (rGH), growth performance, survival Share Link | Plain Format | Corresponding Author (marlina achmad)

190	Sustainable Aquaculture (International)	ABS-258
Application of Eco-Enzyme In An Acid Sulphate Pond for pH Improvement Erni Indrawati (a), Ratnawati Gatta (a*)(b), Amal Aqmal (a), Mitra Mandasari (a), Rezky Syahriana (a), Mutmainna Daeng Masese (a), Muhammad Hamzah (a) a) Bosowa University, Jl. Urip Sumoharjo Km.4, Makassar-Sulawesi Selatan 90231 *ratnawati[at]universitasbosowa.ac.id b) Sekolah Sampah, Jl. Urip Sumoharjo Km.4, Makassar-Sulawesi Selatan 90231 Abstract The sulfat acid Soil contains high pirit (FeS2) and low PH, delaying shrimp growth, which turns the shrimp vulnareable to any diseases. However, there are many potential types of sulfat acid soil found in coastal areas to be developed as shrimp pond, but it requires sufficient handling. One of it is shrimp pond remediation with the calcium and fertilizer to increas its nutrients and pH. Eco-enzym can be used for replacing the calcium and fertilizer, which improves soil structure in eco-friendly way. This research aims at analyzing the correct dose of eco-enzyme, which improves the pH of acid soil in the shrimp pond. The result of this research explains that eco-Enzyme application increases pH soil, either in the surface of the soil that has been through 4 times of reclamation or below at the 50cm depth, where it shows the same pH. Keywords: acid sulphate pond, eco-enzyme, pH, shrimp ponds Share Link | Plain Format | Corresponding Author (Ratnawati Gatta)

191	Sustainable Aquaculture (International)	ABS-6
SURVIVAL RATE, GROWTH AND HEALTH OF SCALLOPED SPINY LOBSTER, Panulirus homarus (Linnaeus 1758) REARED IN CONCRETE TANK FED WITH FRESH FEED AND DRY PELLET Bejo Slamet(a*), Sudewi (a), Wayan Widya Astuti (a), Fahrudin and Nyoman Adiasmara Giri (a) a)Institute for Mariculture Research and Fisheries Extensio BD Gondol, Ds Penyabangan, Kec Gerokgak 81155, Buleleng, Bali Indonesia *Corresponding authors : bedjoselamet62[at]gmail.com Abstract Scalloped spiny lobster (Panulirus homarus) is an high economic value in Asian-Pacific. The purpose of this study was to determine the best feed for spiny lobsters grow out in concrete tank. The study using 12 concrete tank 2.5x2.5x1m in size. Four different feed were used as treatments, i.e.: fresh feed (A), commercial pellet for grouper (B), for shrimp (C), and dry pellet Gondol formula (D). The lobster with initial average of total length (TL) 13.1 cm and body weight (BW) 78g was reared at density 25 ind./tank. Feeding 2x/day with dose 10% of biomass for fresh feed and 2% for dry pellet per day. The samplings were conducted on survival rate (SR), TL, BW, total haemocyte count. The result during 5 month rearing show that the best SR (94.67%) was on treatment D, followed by B (84.25%), C (82.67%) and A (50.67%). The highest final BW on D (204.1g), followed by A (190.5g), B (168.6g) and C (144.1g). The total haemocyte count was 292x104cel/ml almost the same between treatments. The conclusion is dry pellet formula IMRAFE Gondol is more feasible than another feed treatment for rearing of scallop spiny lobster grow out in concrete tank. Keywords: Grow out, Feed, Concrete tank, Panulirus homarus Share Link | Plain Format | Corresponding Author (Bejo Slamet)

192	Sustainable Aquaculture (International)	ABS-266
The Effect of Phyprozyme HP Supplemented Diets on Growth Performance, feed utilization, and Survival rate of Nile tilapia (Oreochromis niloticus) Ayman Alameen Mohammed (a*), Sajidan (b), Agung Budiharjo (b) (a) Department of Fisheries and Wildlife Science, College of Animal Production Science and Technology, Sudan University of Science and Technology, Wehda street 9, Khartoum, Sudan (b) Department of Bioscience, Graduate School, Sebelas Maret University Abstract This study was conducted to know the effect of phyprozyme HP supplemented diets on growth performance and survival rate of Nile tilapia. Phyprozyme HP enzymes are standardized to contain a combination of protease. xylanase. hemicellulose. and phytase. This research used completely randomized design with eight treatments and two replicates T1 control 0.00. T2 0.05. T3 0.12. T4 0.19. T5 0.26. T6 0.33. T7 0.40. and T8 0.47 g phyprozyme HP kg feed. Nile tilapia with the average initial weight of 11.9 g were stocked into hapa nets 70.45.90 cm3 20 fish per hapa net and were fed three times daily 7 00 AM.12 00 Noon. and 16 00 PM at feeding rate of 5% of the total body weight. After 56 days of feeding, the mean final weight MFW. weight gain WG. average daily gain ADG. feed conversion ratio FCR. specific growth rate SGR. and survival rate SR were determined. The results show that there were significant differences on growth performances among dietary treatments. The growth of the fish fed T6 0.33 g showed higher MFW 435.30. WG 84.85. ADG 3.6. SGR 9.45 compared to other treatments. The best feed conversion ratio FCR was achieved with T6. T5 and T7 followed by T3.T4. T2. and T1 but the T8 had the worst FCR. There were no signi&#64257-cant differences in survival rate SR among the eight dietary treatments. Based on the results, it could be concluded that supplementation of Phyprozyme HP in tilapia diets can improve the growth performance and may even have negative effects with higher doses. Keywords: : Enzyme supplement, feed utilization, growth response, Oreochromis niloticus Share Link | Plain Format | Corresponding Author (Ayman Alameen Mohammed)

193	Sustainable Aquaculture (International)	ABS-12
Characteristics and status of water quality in coastal waters after six years of intensive whiteleg shrimp (Litopenaeus vannamei) culture Admi Athirah and Akhmad Mustafa Research Institute for Coastal Aquaculture and Fisheries Extension Abstract This research aims to determine changes in water quality characteristics and status in 2015 and 2021. A total of 12 transects perpendicular to the coastline and 3 transects parallel to the coastline, or as many as 36 station points, were determined to measure and to take sample water in coastal waters of intensive technology whiteleg shrimp ponds in Gantarang Subdistrict, Bulukumba Regency, South Sulawesi Province, Indonesia. Water quality measurements and sampling were carried out in the rainy season in 2015 and 2021. Non-parametric statistics, namely the Mann-Whitney test, were used to determine the difference between each water quality variable at different times. Water quality status was determined using the Storet (Storage and Retrieval) method. The results showed that intensive technology of whiteleg shrimp culture in Gantarang Subdistrict, Bulukumba Regency in 2021 was carried out with a stocking density of 110 ind/m2 to 220 ind/m2 with productivity between 13.9 tons/ha/cycle up to 30.0 tons/ha/cycle with a predicted waste load of 27.68 tons N/year and 6.53 tons P/year. From 2015 to 2021 there was an increase in salinity, ammonia, nitrate, nitrite, phosphate, total suspended solids, and total organic matter and conversely, there was a decrease in temperature, pH, and dissolved oxygen. The status of water quality in the coastal waters of Gantarang Subdistrict, which receives the load of intensive technology whiteleg shrimp pond waste, is classified as Class A or Very good or Complying with quality standards, both measurements in 2015 and after six years, namely in 2021. It is necessary to increase the capacity and capability of the WWTP plots for ponds intensive technology of whiteleg shrimp culture in Gantarang Subdistrict and regularly monitor water quality characteristics and status in coastal waters. Keywords: brackishwater aquaculture, intensive technology, water quality, whiteleg shrimp, Indonesia Share Link | Plain Format | Corresponding Author (Admi Athirah)

194	Sustainable Aquaculture (International)	ABS-274
The Effect of Various Vitomolt on The Meat Quality and Nutrient Retention of The Fattening Mud Crab, Scylla Olivacea Nur Intan Sari (a), Yushinta Fujaya (b), Siti Aslamyah (b*) a) Postgraduate Program, Hasanuddin University, Makassar, Indonesia b) Faculty of Marine Sciences and Fisheries, Hasanuddin University, Makassar, Indonesia *sitiaslamyah1[at]gmail.com siti.aslamyah[at]unhas.ac.id Abstract Mud crab, Scylla olivaceae is a fishery commodity with high economic value and nutritional value. Its marketing is not difficult because of the high demand for exports and seafood restaurants. This study aims to evaluate the effect of various doses of vitomolt and determine the best dose on meat quality and nutrient retention in mud crab fattening culture. This research was conducted at the Educational Farm, Hasanuddin University, Bojo Village, Mallusetasi District, Barru Regency, Indonesia. The test animals used were mud crabs, Scylla olivaceae totaling 240 individuals, 60 each per treatment. The treatments were tested with various doses of vitomolt in the feed, namely 0, 200, 400, 600 mg vitomolt/kg feed. Parameters observed were crab carcass measurement, body chemical composition, nutrient retention consisting of protein, fat and energy retention, and crab water quality. The results showed that by mixing various doses of vitomolt to the feed, the best dose in measuring mud crab carcass was 400 mg/kg feed, while the best dose for nutrient retention was the test results for protein retention and the best dose of energy was 600 mg/kg. The best fat retention is 400 mg/kg feed for mud crab, Scylla olivacea. Keywords: Fattening, meat quality, mud crab, nutrient retention, vitomolt Share Link | Plain Format | Corresponding Author (Nur Intan Sari)

195	Sustainable Aquaculture (International)	ABS-275
The effect of Prebiotics Extracted From Various Natural Ingredients and Combinations of Microorganisms as Probiotics on The Degree of Nutrient Hydrolysis of Milkfish Feed, Chanos chanos Forsskal 1775 Siti Aslamyah(*) Zainuddin & Badraeni Aquaculture Study Program, Department of Fisheries, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar 90245, Indonesia *sitiaslamyah1[at]gmail.com siti.aslamyah[at]unhas.ac.id Abstract Prebiotics are supplements that promote the growth of microorganisms. A high population of microorganisms contributes to producing enzymes that hydrolyze feed nutrients. This study aimed to examine the effect of prebiotics extracted from various natural ingredients and combinations of microorganisms as probiotics on the population of microorganisms, the activity of cellulase enzymes, &#945--amylase, and proteases, as well as the degree of nutrient hydrolysis of milkfish feed. The treatments tested were A. Combination of microorganisms, which are 1. Bacillus sp. and Lactobacillus sp. 2. Rhizophus sp. and Aspergillus sp. 3. Saccharomyces sp. and Trichoderma sp. 4. The mixture of Bacillus sp., Lactobacillus sp., Rhizopus sp., Aspergillus sp., Saccharomyces sp., and Trichoderma sp. B. Various prebiotics extracted from 1. Sweet potato. 2. Kappaphycus alvarezii seaweed. 3. Green peas. 4. Shallots. The results showed that there were differences in the population of microorganisms, the activity of cellulase,&#945- -amylase, and protease enzymes, and the degree of hydrolysis of nutrients in milkfish feed, including protein, fat, crude fiber, and nitrogen free extract in each combination of treatments, which increased with the addition of prebiotics in microorganism culture. Compared to no prebiotics. The highest yield was obtained in treating mixed microorganisms Bacillus sp., Lactobacillus sp., Rhizopus sp., Aspergillus sp., Saccharomyces sp., and Trichoderma sp. with prebiotics sourced from peanuts and sweet potatoes, followed by shallots and seaweed Kappaphycus alvarezii, the lowest in the control treatment. Keywords: The Degree of Nutrient Hydrolysis, feed, microorganism, prebiotic, probiotic Share Link | Plain Format | Corresponding Author (Siti Aslamyah)

196	Sustainable Aquaculture (International)	ABS-21
Immune response of white shrimp (Litopenaeus vannamei) given the crude protein Zoothamnium penaei with different immersion and rearing times as immunostimulants Amalisa(a), Gunanti Mahasri(b*), Kismiyati(b) a) Master Student of Fisheries Science, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, East Java, 60115 Indonesia b) Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, East Java, 60115 Indonesia *mahasritot[at]gmail.com Abstract Increased cultivation activities and pond intensification have the potential to cause problems, namely increasing cases of disease in shrimp by pathogenic organisms. This study aimed to analyze the immune response of white shrimp (Lithopenaeus vannamei) given the crude protein Zoothamnium penaei with different immersion and rearing times. The research was carried out in November-December 2020. The maintenance of vannamei shrimp was carried out at the Anatomy and Aquaculture Laboratory, Faculty of Fisheries and Marine Affairs, Airlangga University, Surabaya. Bacterial Total Plate Count (TPC) testing, bacterial identification and biochemical tests as well as ProPO and SOD activities were carried out at the Jepara BPBAP Laboratory. Analysis of the levels of ProPO and Superoxide dismutase (SOD) using the Two Way ANOVA test and continued with Duncan^s multiple distance test. The results showed that the immersion time using the crude protein immunostimulant Zoothamnium penaei for 10 minutes during the 14-day maintenance period gave a significantly different effect (p<0.05) on the decrease in total bacteria (TPC) of 1.6 x106 CFU/mL, an increase in the enzyme The highest Superoxide Dismutase (SOD) was in the seven-day rearing period with a 20-minute immersion treatment with a value of 0.731 units, while the increase in the Prophenoloxydase (ProPo) enzyme in vannamei shrimp with the highest value was obtained in the 15-minute immersion treatment at a 14-day rearing period with a value of 0.616. units. The duration of immersion and the duration of maintenance using the crude protein immunostimulant Zoothamnium penaei resulted in a decrease in total bacteria (TPC), an increase in the enzyme Superoxide Dismutase (SOD) and an increase in the enzyme Prophenoloxydase (ProPo). There is a close relationship between the maintenance period between the total plate count of bacteria and the enzymes SOD and ProPo. Keywords: Immunostimulant, crude protein Zoothamnium penaei, Vannamei Shrimp, Aquaculture Share Link | Plain Format | Corresponding Author (Amalisa S.Pi)

197	Sustainable Aquaculture (International)	ABS-278
Effect of feeding rate on growth and feed conversion ratio in the cultivation recirculation systems multi tropic model Heriansah (a), Radjuddin Syamsuddin (b), Najamuddin (b), Syafiuddin (c) a. Doctoral Student in Fisheries Science Study Program, Faculty of Marine Science and Fisheries, Hasanuddin University, Indonesia b. Departement of Fishery, Faculty of Marine Science and Fhishery, Hasanuddin University, Makassar, Indonesia c. Departement of Marine Science, Faculty of Marine Science and Fhishery, Hasanuddin University, Makassar, Indonesia Abstract The effects of feeding rate (0.5%, 7.5%, and 10% body weight (BW) per day on the growth, survival rate, and feed conversion ratio of juvenile rabbit fish, Siganus guttatus, were investigated in a water recirculating aquaculture system of Integrated Multi Trophic Aquaculture (IMTA) model involving mussel Perna viridis, sea urchin Diadema sitosum, and seaweed Kappaphycus alvarezii. Triplicate groups of fish were fed an comercial diet (55.4% protein, 14.1% lipid) four meals a day (08 a.m, 12 a.m, 4 p.m, dan 8 p.m) for 12 weeks. Weight gain, specific growth rate, and feed conversion ratio were better with increasing feeding rates with relatively the same survival. Body weight gain, specific growth rate, and feed conversion ratio were not significantly different among the fish fed 5% and 7.5% BW per day but increased significantly in those fed 10% BW per day. Experimental application of the IMTA model shows that although the amount of feed given is higher, it can produce better growth performance and feed utilization Keywords: feeding rate, feed conversion ratio, growth, IMTA, rabbit fish Share Link | Plain Format | Corresponding Author (Heriansah Heriansah)

198	Sustainable Aquaculture (International)	ABS-23
Morphological Discrimination of Tiger Shrimp Penaeus Monodon Between Female and Male Based on Traditional and Truss Morphometric Analyses A Parenrengi (a*), A Tenriulo (a), E Suryati (a,b), S Lante (a), A Nawang (a), and R Rosmiati (a) (a) Research Institute for Brackishwater Aquaculture and Fisheries Extension, Maros, Sulawesi Selatan, Indonesia. * Corresponding author: andi_parenrengi[at]hotmail.com (b) National Research and Innovation Agency, Maros, Sulawesi Selatan, Indonesia Abstract The morphological difference of tiger shrimp, Penaeus monodon from Aceh waters is limited to explore for female and male discrimination using morphometric analyses. The study aimed to evaluate the morphology characters between female and male tiger shrimp using traditional and truss measurements. A total of 57 samples (39 females and 18 males) were analysed using 9 characters for traditional morphometric and 40 characters for truss morphometric. Multivariate analyses were applied to reveal the morphometric variation between male and female populations. The result of principal component analysis indicated that 4 characters, i.e: carapace length (CL), carapace width (CW), rostrum length (RL), and sixth segment length (SL6), were significantly different (P<0.05), while three truss morphological characters, i.e: distance between anterior and posterior carapace, distance between posterior sixth segment to telson end, and distance between anterior and posterior second segment were significantly discriminated (P<0.05) between female and male of tiger shrimp. The overall assignment of female and male individuals into their original population for traditional morphometric was 94.9% and 88.9%, respectively, while for truss morphometric was 87.2% and 88.9%, respectively. The proportion of individuals correctly classified into their original population group was 93.0% for traditional morphometric and 87.7% for truss morphometric characters. Keywords: female- male- tiger shrimp- traditional characters- truss characters Share Link | Plain Format | Corresponding Author (Andi Parenrengi)

199	Sustainable Aquaculture (International)	ABS-279
Growth and survival rate of mud crab crablet, Scylla tranquebarica fed a multi-enzyme supplemented diet Edison Saade1, Sulaeman2 and Ahmad Albar1 Hasanuddin University Abstract Multi-enzyme plays an important role in the optimal growth of mud crab, Scylla tranquebarica. This study aimed to determine the best dose of multi-enzyme on the growth and survival rate of mud crab crablets. Each crablet was kept in a plastic-cup container with a volume of 450 mL filled with 250 mL of seawater that arranged in a 500 L volume fiber tank. The fiber tank is equipped with an aeration system. Multi-enzyme was sprayed to artificial feed about 10 minutes prior feeding at a dose of 0 cc/kg of feed (treatment A), 30 cc/kg of feed (treatment B), and 60 cc/kg (treatment C) and apply for 50 days. The frequency of feeding was twice a day, morning and evening with a dose of 5% per crablet weight. Parameters measured were growth in relative weight (GweR), growth in relative shell width (GwiR), growth in relative shell length (GslR) and survival rate (SR). In addition, several water quality parameters are measured as supporting data. The data is processed descriptively. The results showed that GweR, GwiR, and GslR were increased up to a dose of 30cc/kg of feed and decreased at a dose of 60cc/kg of feed. Survival rates were 100% in all treatment units. Based on the results of this study, it was concluded that the dose of multi-enzyme for the best growth and survival rate of mud crab crablet was 30cc/kg of feed. Keywords: Growth, mud crab crablet, multi-enzyme, and survival rate Share Link | Plain Format | Corresponding Author (Edison Saade)

200	Sustainable Aquaculture (International)	ABS-34
Evaluation of animal protein sources in the formulated diets for abalone Haliotis squamata Asep Ridwanudin (1), Dien Arista Anggorowati (1) 1Research Centre for Marine and Terrestrial Bioindustry, National Research and Innovation Agency (BRIN), Lombok Utara, Nusa Tenggara Barat, Indonesia ase_rid[at]yahoo.com Abstract Protein is a main component in the aquafeed, and it has traditionally been supplied from fish meal. However, other animal protein sources such as shrimp head meal and meat and bone meal are also potential to be used as a protein source to replace fish meal in the fish feed including feed for abalone. Therefore, this study aimed to evaluate alternative protein sources mainly from animals to replace fish meal in the diet on the growth of abalone Haliotis squamata. Four experimental diets were formulated to contain fish meal (FM) as a control diet, shrimp head meal (SM), meat and bone (MBM), and a mixture of fish meal, shrimp head meal and meat and bone meal (MIX). The experimental diets were fed once a day at a level 1.5% of total body weight to triplicate groups of abalone. Two hundred and forty abalone with an initial average weight 3.55 g and shell length 28.59 mm were distributed into 12 rectangular plastic baskets at a density of 20 abalone. After twelve weeks feeding trial, growth parameters of abalone fed fish meal (FM) diet were not significantly different than other diets. Therefore, our findings suggest that fish meal could be totally or partially replaced by shrimp head meal or meat and bone meal. Keywords: Feed- Fish meal- Growth- Protein Share Link | Plain Format | Corresponding Author (Asep Ridwanudin)

201	Sustainable Aquaculture (International)	ABS-43
The Effect of Different Diets on Sperm Quality in The Striped Catfish (Pangasianodon hypophthalmus) Wahyu Pamungkas, Jadmiko Darmawan, Ikhsan Khasani Research Institute for Fish Breeding, Ministry of Marine Affairs and Fisheries, Subang, West Java, Indonesia. Abstract Sperm quality has an important role in the success of cultured fish spawning. The present study evaluated the effects of different diets on sperm quality in the striped catfish (Pangasianodon hypophthalmus) during a 3-month feeding trial. Thirty striped catfish were used and equally divided into two groups (A and B). Group A was fed a commercial diet, while group B was fed a formulated diet containing essential fatty acids. Fifteen males with a bodyweight of 2.59-3.09 kg for each group were used as experimental fish. The motility and velocity of the catfish sperm, fertilization, and hatching rate were examined to evaluate the sperm quality and its association with fertilization and hatching rate. An analysis of sperm motility and velocity has been performed using computer-assisted sperm analysis (CASA). The research data were analyzed by an independent sample T-test. The percentage of motility and progressive motile, curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), and hatching rate (HR) in group A were significantly different from group B. These data suggested that diets containing essential fatty acids affected the sperm quality of the striped catfish. Keywords: striped catfish, diet, male, sperm quality, CASA. Share Link | Plain Format | Corresponding Author (Wahyu Pamungkas)

202	Sustainable Aquaculture (International)	ABS-44
Growth, resistance to vibriosis and tolerance to a stressor of the selected giant freshwater prawn, Macrobrachium rosenbergii Ikhsan Khasani1, Asep Sopian1, Wahyu Pamungkas1 Research Institute for Fish Breeding Abstract The faster growth and higher survival of the giant freshwater prawn (GFP) is an urgent requirement to support the GFP farming. Breeding of the GIMacro (strain of GFP) resulted a fourth generation which growth faster than the based population. The comparative test of the selected GI Macro (SGM), the control of GI Macro (CGM), and the Siratu (SR) were conducted to evaluate the performance of these strains in the larval rearing phage, resistance to vibriosis of the larvae, and tolerance to a stressor (pH, temperature, salinity, and formaldehyde) of the juvenile. The growth (Larvae stage index) of the three populations is not different, there were 10.25, 10.31, and 10.12 for SGM, CGM, and SR, respectively. The survival rate of the SGM larvae is about 59.92%, significantly higher than the CGM (52.67%) and SR (45.85%). The resistance level of SGM larvae to Vibrio infection (72.75%) is not different from the CGM (70.25%) and SR (67.88%). The tolerance level of the SGM, CGM, and SR juvenile to these stressors was relatively high, but there were no different. The data suggested that the selected GI Macro II has a good prospect to be developed in the GFP farming system. Keywords: Growth, prawn, resistance, selected, tolerance Share Link | Plain Format | Corresponding Author (Ikhsan Khasani)

203	Sustainable Aquaculture (International)	ABS-47
Effect of bacteria probiotics on maintaining water quality in the super alkaline shrimp pond water M Atmomarsono(1*), BR Tampangallo(2), Nurbaya(2), Kamaria(2) 1)Researcher, Center of Fisheries Research, National Research and Innovation Agency, Maros, South Sulawesi, Indonesia 2)Researcher, Research Institute for Coastal Aquaculture and Fisheries Extension (RICAFE), Maros, South Sulawesi, Indonesia Abstract This study was aimed at finding out whether bacteria probiotics could work properly in the super alkaline pond water. Eight extensive ponds of about 0.5 ha in size located in Lanrisang district of Pinrang regency were used in this experiment. After pond preparation, each pond was stocked with 20,000 pcs of tiger shrimp postlarvae. Parameters of the pond water quality and the bacterial count (total Vibrio and total bacteria) were monitored every two weeks and analyzed at the Research Institute for Coastal Aquaculture and Fisheries Extension Laboratory in Maros, South Sulawesi. Three bacteria probiotics applied here were Brevibacillus laterosporus BT951, Serratia marcescens MY1112, and Pseudoalteromonas sp Edeep-1 BL542. Treatments tested here were A) The powder-formed and B) The liquid-formed of those three bacteria probiotics. There were four replications in each treatment with a culture period was 75 days. The results showed that there were no significant effects of using a different form of probiotics on maintaining water quality since pond water pH sometimes increases up to 10.2 and Dissolved Oxygen often decreases to less than 2 mg L-1. Consequently, the production and survival rate of the cultured shrimp were much lower than those of the average South Sulawesi brackishwater ponds. Keywords: Super alkaline water, probiotic, tiger shrimp, powder, liquid Share Link | Plain Format | Corresponding Author (Muharijadi Atmomarsono)

204	Sustainable Aquaculture (International)	ABS-60
The use of superior common carp fish for better aquaculture Didik Ariyanto Research Institute for Fish Breeding Abstract Fish farming is an economic business activity that has been carried out for a long time in Indonesia. One of the most popular commodities cultured is common carp. In 2001, common carp production was 418,910 MT, equivalent to 63.5% of Indonesia^s national production. However, the existence of obstacles in common carp culture, especially the outbreak of KHV in 2002 and the decrease of genetic quality of fish, indicated by slower growth of fish, resulted a significant decrease of common carp production. The use of lower genetic quality of fish causes the efficiency of the feed used is getting lower, indicated by the high value of the FCR. In-efficient use of feed has an impact on increasing feed waste in culture environment. In order to increase the common carp production, several varieties of superior common carp have been released for the farmer. Empirically, superior fish carp has a better growth rate and more efficient in the use of feed. Culture of these superior varieties impacted to increase the national production of common carp, by an average of 8.7%/year. In the other hand, the quality of the culture environment is keep in good because of the small amount of feed waste produced. Keywords: superior common carp, better aquaculture Share Link | Plain Format | Corresponding Author (Didik Ariyanto)

205	Sustainable Aquaculture (International)	ABS-70
Selection of bacteria degrading carbohydrate, protein, and cellulose from the intestines of Rabbitfish, Siganus guttatus Kamaruddin, Haryati, Siti Aslamyah, Yusri Karim, Sri Rejeki Hesti Mulyaningrum dan B.R. Tampangallo Research institute for Brackish Aquaculture and Fisheries Extension Abstract Rabbitfish is classified as herbivorous fish by utilizing plants as a staple food, so it has the potential to contain microflora in the digestive tract. The study aimed to select bacteria from the intestines of Rabbitfish as probiotic candidates for degradation of feed ingredients. At the beginning of the study conducted microbial dissolution of the intestines of 10 Rabbitfish measuring weight and body length 127 20.1 g and 191.4 1.17 cm, healthy, and not deformed obtained from the waters around the island of Salemo, Regency. Pangkep. At this stage successfully isolated 72 isolates. Then do a selection of bacteria based on the activity of extracellular enzymes by testing the hydrolysis ability of starch using Zobel media adding 1% soluble starch, protein hydrolysis using Zobel media adding 1% skim milk, cellulose hydrolysis using Zobel media adding 1% CMC. The ability of the insulator to hydrolyze starch ranges from 0.4 cm- to protein to 2.4 cm cellulose to 1.5 cm. Based on these results, 10 isolates were selected as probiotic candidates, namely 411, 413, 422, 427, 430, 434, 437, 452, and 455. The next stage of the pathogenicity test of 10 selected isolates. Each isolate with a density of 106 CFU / mL was injected into 5 juveniles of healthy Rabbitfish intramuscularly as much as 0.1 mL. Monitoring is carried out daily for 10 days after infection, to evaluate clinical signs of the disease in test fish. The results obtained showed that no symptoms of pain, injury, or death were found, so it was concluded that the bacterial isolate was not pathogenic. Keywords: Rabbitfish, microflora, probiotics, enzymes, pathogens Share Link | Plain Format | Corresponding Author (Kamaruddin Kamaruddin)

206	Sustainable Aquaculture (International)	ABS-72
Performance growth and survival of Hemibagrus Nemurus with probiotic application on aquaponics Yosmaniar, Imam Taufik, Lies Setijaningsih and Yohanna R Widyastuti2stutai National Research and Innovation Agency, Indonesia Abstract Hemibagrus nemurus is a freshwater catfish commodity that has high economy value and favored by the community. The purpose of study was to evaluate the growth and survival of Hemibagrus nemurus in aquaponics with the application of probiotics. The research was conducted in Sukawening Dramaga Bogor. Using 6 units of tarpaulin ponds measuring 2.0 X 3.0 x 0.8 m filled with 3 M 3 of water, each pool is equipped with an aquaponics unit with an upstream system using lettuce and eggplant. The experiments, namely using RIFA probiotics and without using probiotics with 3 replication. The test fish used is of 16.5 sd 0.2 g/individu. The RIFA probiotics used at a dose of 10 mL/M3. Given every 5 days. Fish were given commercial feed of 4 % at 50 days of rearing and then 3% until the end of the study with feeding times/day (morning and afternoon). The test parameters were SGR, survival, absolute weight. FCR, and water quality. The result showed that the application of probiotic RIFA can increase the growth and survival of the fish, improve water quality and increase plant productivity Keywords: probiotic, aquaponic,Hemibagrus nemurus Share Link | Plain Format | Corresponding Author (YOSMANIAR -)

207	Sustainable Aquaculture (International)	ABS-77
OPTIMIZATION OF SURVIVAL AND GROWTH OF Tilapia (Oreochromis niloticus) IN INTEGRATED RICE-FIELD CULTURE WITH APPLICATION OF FENTINE ACETATE AS MOLUSCICIDE Lies Setijaningsih (a*) , Imam Taufik (a), Dewi Puspaningsih (a) a) Research Institute for Freshwater Aquaculture and Fisheries Extention *liessetijaningsih[at]yahoo.com Abstract Fentin acetate is used as a pesticide in rice fields to eradicate pests. Research with physiological factors approach is expected to increase the survival and growth of tilapia. The purpose of this study was to determine the impact of exposure of fentin acetate with different concentrations on the biological performance and hematological characteristics of tilapia (Oreochromis niloticus). The research land used 16 rice fields with an area of 30 m2 each (6 x 5 m). The research design used was a completely randomized design with four treatments and three replications, different concentration of fentin acetate, namely: A. 0.00- B. 0.375- C. 0.75- and D. 1.5 g/L for 21 days experiment. The results showed that fentin acetate in treatment C resulted in the best growth of tilapia. It was noted that absolute weight gain, daily growth rate and productivity were significantly different (P<0.05) with treatments A, B and D. The highest value in treatment C was 20.53 g for absolute weight gain, 5.43 for daily growth rate and 501.58 g for productivity. The survival rate in treatments B and C were not significantly different (P>0.05) as well as treatments A and D. The concentration of molluscicide fentin acetate at a dose of 0.75 g/L affected the decrease in the hematological characteristics of tilapia. Keywords: fentin acetate, rice-filed culture, tilapia Share Link | Plain Format | Corresponding Author (Lies Setijaningsih)

208	Sustainable Aquaculture (International)	ABS-81
Preliminary Study of Using High Level of Ulva Meal as a Feed Ingredient for Nursery Stage of Cultured Golden Rabbitfish, Siganus guttatus Asda Laining (a*), SRH Mulyaningrum (a), MC Undu (b), Lideman (c), Ramadhan (a) (a) Research Institute for Coastal Aquaculture and Fisheries Extension, Ministry of Marine Affairs and Fisheries, Jl. Makmur Dg. Sitakka No.129, Maros 90512, South Sulawesi, Indonesia *asdalaining[at]yahoo.com (b) Polytechnic of Marine and Fisheries of Jembrana, Ministry of Marine Affairs and Fisheries, Desa Pengambengan, Kecamatan Negara, Kabupaten Jembrana, Bali, Indonesia (c) Brackishwater Aquaculture Development Center, Ministry of Marine Affairs and Fisheries, Kecamatan Galesong Selatan, Kabupaten Takalar, South Sulawesi, Indonesia Abstract A 15-weeks feeding trial was conducted to evaluate the effect of utilizing a high level of seaweed Ulva meal as a dietary feed ingredient for the nursery stage of cultured golden rabbitfish, Siganus guttatus on the growth and biochemical composition of the fish body. Two tested diets as the treatment were evaluated with four replicates. One diet was formulated with a high level of Ulva meal at a rate of 30% combined with a high level of soybean as the protein source whilst another diet used fish meal as the major protein source (FP). Cultured rabbitfish (3rd generation) with a mean initial body weight of 5.4 g and length of 6.3 cm were stocked into 8 of 120 L fiberglass tanks with a density of 20 fishes per tank. Fish were fed to satiation 4 times a day at 0700, 1000, 1300, and 1600. Sampling to monitor the fish growth was performed every 3 weeks for 15 weeks. The growth rate showed a linear pattern in both FP and PP diets. Fish fed the PP diet grew faster than those fed with FP diet. Weight gain of fish fed PP diet was 453% higher compared to those fed with FM (343%). A similar trend was observed on SGR where fish fed the PP diet had a higher SGR of 1.40 %/d compared to the PF diet (1.22%/d). Feed consumption of fish fed the PP diet tended to be higher approximately 49.8 g than fish fed FP diet (30.7 g) further resulting in a lower FCR of the FM diet at the rate of 1.3 compared to 1.6 for the PP diet. Survival of the fish fed FP and PP diets were 92.5 and 100%, respectively. Fish fed the FM diet contained a higher ash content compared to those fed with the PP diet (14.9 vs 10.1%). In contrast, fish fed the PP diet had a slightly higher fiber content of 8.5% compared to the FP diet (7.6%). Inclusion of Ulva meal at 30% combined with a high level of soybean meal as a protein source in a low fishmeal diet produced better growth performances of rabbitfish during the nursery stage in floating pond cages. Keywords: low fishmeal diet, high plant-supplemented diet, herbivorous, seaweed Share Link | Plain Format | Corresponding Author (Asda Laining)

209	Sustainable Aquaculture (International)	ABS-84
The productivity phenomenon of tilapia floating net cages in Jatiluhur Reservoir in line with the Citarum Harum program Estu Nugroho (a), Lies Setijaningsih(b), Dewi Puspaningsih(b), Raden Roro Sri Pudji Sinarni Dewi(b) a) Fisheries Research Center, Jl Raya Jakarta-Bogor, Cibinong b) Research Institute for Freshwater Aquaculture and Fisheries Extention, Jl. Sempur No. 1, Bogor E-mail: engroho[at]yahoo.com Abstract The research was conducted to determine the productivity conditions of tilapia cultivation in the field along with the implementation of reducing the number of floating net cages. Production observations of tilapia culture were carried out on net cages owned by fish farmer in the Jatiluhur Reservoir from 2012 to 2019. The first period-P1 (2013-2015) was before the reduction in the number of net cages, the culture used a double net system (feed only for carp in top net) and hybrid system (double net + feed for tilapia). The second period-P2 (2016-2019) when the program started and as the number of KJAs decreased, KJA culture was carried out with a self-maintenance system (100% feeding for tilapia). The value of fish harvest weight ranged from 96.39-102.47 g (P1) and 196.84-278.33 g (P2). The range of survival rate is between 56.99-78.79% (P1), and 60.92-80.41% (P2). The feed efficiency value obtained in the P2 period was 75.77-86.34% while for P1 it was not defined because it was not a 100% feed system (independent). The daily weight gain, productivity and multiple values were as follows: 0.58-1.0 g/day, 0.14-0.45 g/m2, and 2.35-4.56x (P1)- 1.43-3.00 g/day, 1.92-2.89 kg/m2 and 6.77-10.80x (P2). The monitored water quality parameters were still within a tolerable range for tilapia floating net cage culture. Keywords: tilapia, floating net cages, biological parameter Share Link | Plain Format | Corresponding Author (Dewi Puspaningsih)

210	Sustainable Aquaculture (International)	ABS-93
Growth and feed conversion of rabbitfish Siganus canaliculatus Park, 1797 at differen density H Y Azis1, Y Fujaya1, S Aslamyah1, Tresnati1,2, A Tuwo2,3, A D Bestari2 1Fisheries Department, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, Indonesia. 2Multitrophic Research Group, Universitas Hasanuddin, Makassar, Indonesia. 3Marine Science Department, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar, Indonesia. Abstract The rapid increase in population and environmental degradation that impacts food security is a central issue that will continue to strengthen in the future. One of the efforts that can be made to improve food security is the development of environmentally friendly cultivation, such as multitrophic cultivation. Feed is an essential input in cultivation. In multitrophic cultivation, this feed input is substituted with natural feed. Thes study aims to examine whether there are differences in growth and feed conversion of rabbitfish Siganus canaliculatus reared at different densities in controlled tanks fed natural food such as seaweed Gracilaria changii. Parameters observed were growth and feed conversion at three density levels (5, 3.5, and 1.5 rabbitfish per m2) with nine replications, using 27 tanks of 2 x 2 x 1 m. The results showed the significant differences between densities in total and relative length growth, and the total and relative weight. Feed conversion was not significantly different in all densities. Although there are differences in growth in length and weight, this difference is probably not caused by dietary factors. It is suspected that this difference is caused by the size of the rabbitfish, which is quite diverse, and/or other biological factors Keywords: Density, seaweed, Gracilaria changii, controlled tank, feed efficiency Share Link | Plain Format | Corresponding Author (Aprilianti Dewi Bestari)

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