REGENERATION OF KAPPAPHYCUS ALVAREZII SEAWEED RESULTING FROM THE TRANSFORMATION OF THE PACS GENE (Citrate synthase) TO INCREASE RESISTANCE TO ENVIRONMENTAL CONGESTION IN FLOATING NET CAGES IN PANGKEP WATER
Emma Suryati1,2), Andi Tenriulo1,2)Rosmiati1,2) , Andi Parenrengi1,2), and Sri Rejeki HM1,2

1) Research Institute for Brackhiswater Aquaculture and Fisheries Extention
2) Center of Fisheries Research, National Research and Innovation Agency, Maros, South Sulawesi

Abstract

The transformation of the PaCs (Citrate synthase) gene in seaweed K.alvarezii has been successfully performed in vitro. To improve the survival of the transformation of the Citrate synthase seaweed gene, it is carried out: (1) Regeneration of the results of transformation in culture chamber in the laboratory- (2) Acclimatization in green net (MS 100 um) size 50x50x50 cm, density 200 explants maintained for 2 weeks, transferred to the blue net with a size of 50x50x50 cm (MS 200 um) density 200 explants maintained for 4 weeks, then maintenance by the longline method using a double rope with a distance of 15 Cm on floating net cages. Measurement of weight, length, and water quality, is carried out at intervals of 2 weeks. Analysis of PaCs gene integration with PCR using Primary PaCS-F and PaCS-R. The results showed that the DGR in the culture chamber was 0.11% to 0.18%/day. Acclimatization on green nets indicates a DGR of 0.62 to 1.3% / day, and on blue nets, DGR increases to 2.18% /day. Seaweed enlargement using the longline method shows a DGR of 1.42-4.3%/day. The results of the integration of the Citrate synthase gene in seaweed showed the band at a position corresponding to the promoter fragments of 1300 bp PaCS-F and PaCS-R in the CaMV 35S vector. Based on these results the PaCS gene has been successfully inserted into the seaweed K.alvarezii

Keywords: Acclimatization, Kappaphycus alvarezii, floating net cage, long line, growth rate, gene PaCS (Citrate synthase)

Topic: Marine and Fisheries Biotechnology (International)