Chemical profile, toxicity, cytotoxicity and antioxidant potencies of ethylacetate extract of soft coral Lobophytum sp. growing in South East Sualwesi sea
Sahidin I1, Adryan Fristiohady1, Muhammad Hajrul Malaka1, Baru Sadarun2, Nur Syifa Rahmatika3, Agung Wibawa Mahatva Yodha1,4, Andini Sundowo5

1Faculty of Pharmacy, Universitas Halu Oleo, Kendari, INDONESIA
2Faculty of Marine Sciences and Fisheries, Universitas Halu oleo, Kendari, INDONESIA
3Faculty of Medicine, Universitas Halu Oleo, Kendari, INDONESIA
4Health Polytechnics of Bina Husada, Kendari, INDONESIA
5Research Centre for Chemistry, National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten, INDONESIA

Abstract

Soft coral of Lobophytum sp. grows a lot in the seas of South East Sulawesi, Indonesia. However, no information has been found regarding the study of chemical and pharmaceutical aspects of this genus fom this area. Therefore, the purpose of this article is to present the results of a general study on the pharmaceutical and chemical aspects of Lobophytum sp. The sample collected from the water of Saponda Island, than extracted by ethylacetate. The chemical content was analyzed using phytochemical tests and LCMSMS. Antioxidant potency was evaluated by DPPH radicals and ABTS methods, cytotoxicity towards MCF7 cell line using MTT assays, and toxicity by BSLT. The results showed that the ethylacetate extract has potential as antioxidant and cytotoxic against MCF7 cell lines which indicated by IC50 value. The antioxidant potential was revealed by IC50 (g/L) 84.99 (DPPH) and 67.99 (ABTS). In addition, the cytotoxicity presented by IC50 (g/L) 37.35 and 50.07 for BSLT. Those activities supported by the qualitative phytochemical screening that exhibited the extract contains terpenoids, steroids, alkaloids and phenolics compounds. LCMSMS data indicated that ethylacetate extract has arachidonic acid, edultin, fragransol D, neociwujiaphenol, pluviatelol and some unidentified compounds with molecular formulas C21H26O8, C23H47N3O, C32H51NO7, C31H47NO3, C33H51NO3.

Keywords: Lobophytum sp., ethylacetate extract, chemical profile, antioxidant, toxicity

Topic: Marine and Fisheries Biotechnology (International)