Reproductive performance of male tiger prawns (Penaeus monodon) fed trash fish and fermented sea cucumber (Holothuriadae) offal- study of gonadal morphology and histology
Andi Aliah Hidayani (a), Yushinta Fujaya (a*), Achmad Radhifan Imam Wahyudi Duni (a), Muh Darhan Hibaturrahman (a), Waiho Khor (b), Hanafiah Fazhan (b), Benny Diah Madusari (c), Haruna (d)

a) Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Tamalanrea, Makassar, South Sulawesi Indonesia.
b) Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, 21030, Terengganu, Malaysia
c) Fisheries Faculty, Pekalongan University, Jl. Sriwijaya 3, Bendan, Pekalongan, Central Java Indonesia
d) Takalar Brackish Water Cultivation Fisheries Center, Mappakalompo Village, Galesong District, Takalar Regency, South Sulawesi Indonesia

Abstract

This study aimed to compare the effect of various doses of fermented sea cucumber (Holothuridae) offal in trash fish on the development of gonads and sperm of tiger prawns (Penaeus monodon). There were 36 adult male tiger prawns with an average weight of 83.61 g used in this study. Completely Randomized Design (CRD) with 4 treatments and 3 replications used in this study. treatment A was feeding trash fish without fermented sea cucumber as a control, treatment B was feeding trash fish mixed with 25 ml fermented sea cucumber innards, treatment C was giving trash fish feed mixed with 50ml fermented sea cucumber, and treatment D was feeding trash fish mixed with 75ml fermented sea cucumber. The development of gonads and spermatozoa was observed morphologically and histologically. The results showed that feeding trash fish mixed with fermented sea cucumber offal with different doses had no significant effect (p>0.05) on the gonadal maturity index of male tiger prawns but had a significant effect (p, <0.05) on the number of spermatozoa. The highest number of spermatozoa in terminal ampule was obtained in treatment C, namely 114.83 x 106, and the lowest yield was obtained in treatment A that is 30.75 x 106.

Keywords: sea cucumber, Penaeus monodon, gonad, spermatozoa, histology, morphology.

Topic: Marine and Fisheries Biotechnology (International)