Application of 17alpha-methyltestosterone to enhance broodstock maturation of the domesticated black tiger shrimp (Penaeus monodon): A comparative study of injection method and oral method R Rosmiati, S Lante, A Parenrengi, E Suryati, A Tenriulo, and A Nawang
1Research Institute for Brackishwater Aquaculture and Fisheries Extension, Maros, South Sulawesi, Indonesia
2National Research and Innovation Agency, Maros, South Sulawesi, Indonesia
Abstract
The effects of 17alpha-methyltestosterone on spermatophore quality of tiger shrimp Penaeus monodon were investigated using injection and oral method. The experiment was designed to compare the oral administration group and injection group with two replications at 38-day experimental period. Captive male broodstock of tiger shrimp weighing 64.8-71.5 g collected and stored in a 10,000 m3 concrete tank (20 shrimp/tank). Male were injected and oral administrated with 17alpha-methyltestosterone at the dose of 300 ng/100 g body weight. The injection of 17alpha-methyltestosterone shows the percentage of mature broodstock and the percentage of normal spermatozoa count 1.07 and 1.38 higher and significantly differs from oral administration through mixing in feed at the maturation stage. However, at the time of the 1st re-maturation and 2nd re-maturation of both the methods, the number of mature broodstock, the weight of the spermatophores, the number of spermatozoa and the percentage of the normal spermatozoa did not show any difference. Based on the comparison of the two methods used, it is obtained that the administration of the hormone 17alpha-methyltestosterone can be done both through mixing in feed and through injection, but the administration of hormones through injection gives better results that the broodstock matures first simultaneously.
