Use of Sonneratia alba leaf extract solution for White Spot Syndrome Virus prevention in tiger shrimp postlarvae Nurhidayah, Muliani, Nurbaya, Muharijadi Atmomarsono
National Research and Innovation Agency
Abstract
Abstract. White Spot Syndrome Virus is one of the leading causes of mass mortality in tiger shrimp culture. This study aimed to determine the effect of different immersion times in 1% Sonneratia alba extract on the survival of tiger shrimp Postlarvae after infection with WSSV. The study used a completely randomized design with treatments, namely A) 12 hours of immersion- B) 24 hours- C) 36 hours- D) 48 hours- E) 60 hours- F) 72 hours- G) Positive control (infected with WSSV without mangrove extract)- and H) Negative control (without WSSV infection and mangrove extract), with three replications each treatment. Stocking density of tiger prawn postlarvae 20 pcs/jar. All immersion methods were performed before WSSV infection (concentration 0.1%). They were feeding three times a day, 20% of the total biomass per day. Post-larvae mortality of tiger prawns was observed every day for eight days. Water quality parameters, including Total Organic Matter, NH3-N, and NO2-N, were measured at the beginning and end of the study. The results showed the highest survival rate (85.0%) in treatment F, while the lowest (0%) was positive control (treatment G). The more extended immersion in the herbal extracts increased the shrimp^s protection against WSSV.
