Application of dsRNA VP24 WSSV by Oral Administration at Different Larval Stages of Tiger Shrimp Penaeus monodon
A. Tenriulo(a*), A. Parenrengi(a), S. Lante(a), E. Suryati(a,b), R.Rosmiati(a), and Agus Nawang(a)

(a)Research Institute for Brackishwater Aquaculture and Fisheries Extension, Maros, Sulawesi Selatan, Indonesia
*Corresponding author: anditenriulo[at]yahoo.com
(b)National Research and Innovation Agency, Maros, Sulawesi Selatan, Indonesia

Abstract

This study aimed to evaluate the effect of dsRNA VP24 application on different stages of larvae. The cloned recombinant bacteria carrying the construct of promoter T7VP24 grew in LB media for mass production. The bacteria were inactivated using the heat-killed bacteria method by immersion at 80o C for 5 minutes. The inactivated bacteria were mixed with larval commercial feed. The nauplii were produced from broodstock from Aceh waters and reared until postlarva 12 (PL12). The feed containing the dsRNA vaccine was applied to a different stage of larva, i.e: starting from zoea 1 (A), mysis 1 (B), PL 1 to the PL 12 (C), and control without dsRNA (D). The PL 12 were challenged with WSSV by immersion method and morphological characters were assessed. Results showed that inactivated bacteria were effectively done by immersion at 80o C for 5 minutes without damaging of dsRNA construct in the plasmid. The survival rate was significantly influenced by different stages of larvae (P 0.05), in which the highest survival (26.0%) was obtained from mysis. The highest value of morphological characters (92.3) was also inhibited in the mysis. The results suggested that the dsRNA vaccine for larvae could be started to be applied in the mysis stage.

Keywords: dsRNA, larval stage, P.monodon, VP24-WSSV

Topic: Marine and Fisheries Biotechnology (International)