Analysis of the immune parameters of shrimp fed diets supplemented with Phaeodactylum tricornutum. Ince Ayu K.Kadriah*1 Muliani1, Nurbaya1,Kamaruddin1, Rafly Ian Saharani2, Patang2 and Andi Sukainah2
1 Fisheries Research Center. National Research and Innovation Agency of Republic Indonesia. Cibinong West Java.
2 Faculty of Engineering, Makassar State University
Jl. Dg. Tata Raya Parangtambung Makassar
Abstract
Currently, one of the approaches that is widely used in efforts to prevent shrimp disease is to increase shrimp immunity. The use of several natural ingredients has been tried to improve the performance of shrimp immun parameters, including the use of mangrove leaf extract and microalgae extract. In this study, supernatant extract and ethyl acetate extract from the microalgae Phaeodactylum sp will be used as immunostimulator to improve the immune system function of shrimp. Microalgae supernatants and ethyl acetate extracts are mixed into the feed formula as much as 35% of the feed weight. The test animal used was a 35-day-old shrimp juvenile. Supplemented feeding is carried out for 30 days before the challenge test. During the 30-day maintenance process, haemolimph sampling was carried out every 5 days to determine the THC value and Pro-PO activity. After the infection is carried out, haemolimph sampling is carried out once every 3 days for 7 days of maintenance. The calculation of shrimp survival after infection is carried out daily. The results showed that the addition of Phaeodactylum sp supernatant extract to shrimp feed was able to stabilize and maintain shrimp immune parameters when pathogenic bacterial infection occurred.
Keywords: Phaeodactylum tricornutum, shrimp disease, Vibriosis, Immune system
