Performance of Aquatic Plant Bacopa caroliniana as fish diseases prevention treatment for aquaculture in vitro test Media Fitri Isma Nugraha1, Mia Melvina2, Hessy Novita3, Muh Alias L. Rajamuddin4, Wening Enggarini5, Dwi Budiyanto Trisnoharjono6, Berna Elya2
1 Research Centre for Pharmaceutical Ingredients and Traditional Medicine-National Research and Innovation Agency of Indonesia
2 Faculty of Pharmacy, University of Indonesia, Depok 16424 West Java, Indonesia
3 Research Centre for Veterinary -National Research and Innovation Agency of Indonesia, Jakarta Indonesia
4 Pangkep State Polytechnic of Agriculture, South Sulawesi, 90655, Indonesia.
5 Research Center for Genetic Engineering - National Research and Innovation Agency
6 Research Center for Marine and Land Bioindustry- National Research and Innovation Agency of Indonesia
Abstract
Abstract:
The genus Bacopa a type of aquatic plant that has been widely used in medicine Ayurvedic medicine. The performance of its bioactive compounds used as an antibacterial agent was observed to control fish diseases. Objective, This study was to reveal the content of antioxidant and antibacterial active compounds of Bacopa caroliniana and their effectiveness in controlling the growth of Aeromonas hydrophila, Streptococcus agalactiae, Flavobacterium columnare, Edwardsiella ictaluri, using in vitro methods. Materials and Methods, Bioactive compounds was extracted from Bacopacaroliniana by employing Ultrasound-Assisted Extraction. Extracts were screened qualitatively for antioxidant activity and then quantified to measure in vitro antioxidant activities using the 2.2 diphenyl 1 picrylhydrazyl DPPH radical scavenging assay and the ferric-reducing antioxidant power FRAP assay. The evaluation of chemical compounds was conducted using GCMS. Antibacterial activity was determined by the paper disc diffusion method and microdilution. Results, Bacopa caroliniana extracts were found to contain alkaloids, tannins, flavonoids, saponins, terpenoids, and glycosides. GCMS profiles showed that the extract had 13 identified compounds. Bacopa caroliniana herbal extract has strong antioxidant activity with IC50 value50 microgram per mL i.e. 40.37 microgram per mL. While, Bacopa caroliniana extract inhibited the growth of Aeromonas hydrophila, Streptococcus agalactiae, Flavobacterium columnare bacteria, but not for Edwardsiella ictaluri. Conclusion, The significant potential of secondary metabolites from Bacopa carolinianaindicates an opportunity as a new potential candidate for aquatic plants that have high antioxidant, antibacterial, and anti inflammatory activities.
