Immune response of white shrimp (Litopenaeus vannamei) given the crude protein Zoothamnium penaei with different immersion and rearing times as immunostimulants Amalisa(a), Gunanti Mahasri(b*), Kismiyati(b)
a) Master Student of Fisheries Science, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, East Java, 60115 Indonesia
b) Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, East Java, 60115 Indonesia
*mahasritot[at]gmail.com
Abstract
Increased cultivation activities and pond intensification have the potential to cause problems, namely increasing cases of disease in shrimp by pathogenic organisms. This study aimed to analyze the immune response of white shrimp (Lithopenaeus vannamei) given the crude protein Zoothamnium penaei with different immersion and rearing times. The research was carried out in November-December 2020. The maintenance of vannamei shrimp was carried out at the Anatomy and Aquaculture Laboratory, Faculty of Fisheries and Marine Affairs, Airlangga University, Surabaya. Bacterial Total Plate Count (TPC) testing, bacterial identification and biochemical tests as well as ProPO and SOD activities were carried out at the Jepara BPBAP Laboratory. Analysis of the levels of ProPO and Superoxide dismutase (SOD) using the Two Way ANOVA test and continued with Duncan^s multiple distance test. The results showed that the immersion time using the crude protein immunostimulant Zoothamnium penaei for 10 minutes during the 14-day maintenance period gave a significantly different effect (p<0.05) on the decrease in total bacteria (TPC) of 1.6 x106 CFU/mL, an increase in the enzyme The highest Superoxide Dismutase (SOD) was in the seven-day rearing period with a 20-minute immersion treatment with a value of 0.731 units, while the increase in the Prophenoloxydase (ProPo) enzyme in vannamei shrimp with the highest value was obtained in the 15-minute immersion treatment at a 14-day rearing period with a value of 0.616. units. The duration of immersion and the duration of maintenance using the crude protein immunostimulant Zoothamnium penaei resulted in a decrease in total bacteria (TPC), an increase in the enzyme Superoxide Dismutase (SOD) and an increase in the enzyme Prophenoloxydase (ProPo). There is a close relationship between the maintenance period between the total plate count of bacteria and the enzymes SOD and ProPo.
Keywords: Immunostimulant, crude protein Zoothamnium penaei, Vannamei Shrimp, Aquaculture
