The effect of Prebiotics Extracted From Various Natural Ingredients and Combinations of Microorganisms as Probiotics on The Degree of Nutrient Hydrolysis of Milkfish Feed, Chanos chanos Forsskal 1775 Siti Aslamyah(*) Zainuddin & Badraeni
Aquaculture Study Program, Department of Fisheries, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Makassar 90245, Indonesia
*sitiaslamyah1[at]gmail.com siti.aslamyah[at]unhas.ac.id
Abstract
Prebiotics are supplements that promote the growth of microorganisms. A high population of microorganisms contributes to producing enzymes that hydrolyze feed nutrients. This study aimed to examine the effect of prebiotics extracted from various natural ingredients and combinations of microorganisms as probiotics on the population of microorganisms, the activity of cellulase enzymes, α--amylase, and proteases, as well as the degree of nutrient hydrolysis of milkfish feed. The treatments tested were A. Combination of microorganisms, which are 1. Bacillus sp. and Lactobacillus sp. 2. Rhizophus sp. and Aspergillus sp. 3. Saccharomyces sp. and Trichoderma sp. 4. The mixture of Bacillus sp., Lactobacillus sp., Rhizopus sp., Aspergillus sp., Saccharomyces sp., and Trichoderma sp. B. Various prebiotics extracted from 1. Sweet potato. 2. Kappaphycus alvarezii seaweed. 3. Green peas. 4. Shallots. The results showed that there were differences in the population of microorganisms, the activity of cellulase,α- -amylase, and protease enzymes, and the degree of hydrolysis of nutrients in milkfish feed, including protein, fat, crude fiber, and nitrogen free extract in each combination of treatments, which increased with the addition of prebiotics in microorganism culture. Compared to no prebiotics. The highest yield was obtained in treating mixed microorganisms Bacillus sp., Lactobacillus sp., Rhizopus sp., Aspergillus sp., Saccharomyces sp., and Trichoderma sp. with prebiotics sourced from peanuts and sweet potatoes, followed by shallots and seaweed Kappaphycus alvarezii, the lowest in the control treatment.
Keywords: The Degree of Nutrient Hydrolysis, feed, microorganism, prebiotic, probiotic