The Effect of Different Diets on Sperm Quality in The Striped Catfish (Pangasianodon hypophthalmus) Wahyu Pamungkas, Jadmiko Darmawan, Ikhsan Khasani
Research Institute for Fish Breeding, Ministry of Marine Affairs and Fisheries, Subang,
West Java, Indonesia.
Abstract
Sperm quality has an important role in the success of cultured fish spawning. The present study evaluated the effects of different diets on sperm quality in the striped catfish (Pangasianodon hypophthalmus) during a 3-month feeding trial. Thirty striped catfish were used and equally divided into two groups (A and B). Group A was fed a commercial diet, while group B was fed a formulated diet containing essential fatty acids. Fifteen males with a bodyweight of 2.59-3.09 kg for each group were used as experimental fish. The motility and velocity of the catfish sperm, fertilization, and hatching rate were examined to evaluate the sperm quality and its association with fertilization and hatching rate. An analysis of sperm motility and velocity has been performed using computer-assisted sperm analysis (CASA). The research data were analyzed by an independent sample T-test. The percentage of motility and progressive motile, curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), and hatching rate (HR) in group A were significantly different from group B. These data suggested that diets containing essential fatty acids affected the sperm quality of the striped catfish.
