Page 1 (data 1 to 30 of 84) | Displayed ini 30 data/page
1 2 3 NEXT >>

1	Biomedical Science	ABS-6
EFFECTIVENESS OF BINTARO (Cerbera mangos) LEAVES INFUSION ON INSTAR III LARVAS of Musca domestica Lutfhi Nurlaela(1*), Tsana Makarim Dika Nurulloh(3), Siska Telly Pratiwi(2), ,Emma Mardliyah H(1) 1) Departement of Parasitology, Jenderal Achmad Yani University, Indonesia, Cimahi *ufifuadi[at]gmail.com 2)Departement of Microbiology, Jenderal Achmad Yani University, Indonesia, Cimahi 3) Faculty of Medicine, Jenderal Achmad Yani University, Indonesia, Cimahi Abstract The house fly (Musca domestica) is one of the most common flies around the house. Musca domestica acts as a disease vector due to pathogenic agents it carried from their habitat in carcasses, feces, sewage, or animal waste to human dwellings. The use of chemical or synthetic insecticides can cause resistance to M. domestica, and pollutes the environment. Efforts are being made to overcome by using natural insecticides which has active substances that can interfere with the growth and development of M. domestica larvae such as bintaro leaves (Cerbera mangos). The purpose of this study was to determine the effectiveness of Bintaro leaf infusion against M. domestica larvae. This research is an experimental study using Bintaro leaf infusion in various concentrations, namely 30%, 50%, 70%, and 90%. The results showed that 30% concentration of infusion could kill 30% of larvae and the average mortality continued to increase to 76,5% at 90% concentration. The results of Oneway ANOVA test showed that 70% and 90% concentrations had the same effectiveness, and the results of the probit analysis showed that the LC50 value was 74% while the LC90 value was 137%. This proves that all concentrations of bintaro leaf infusion can kill the third instar larvae of M. domestica. Therefore, Bintaro leaf infusion can be used by the community as a natural larvicide. Keywords: Cerbera manghas, Housefly, Larvacides, Musca domestica. Share Link | Plain Format | Corresponding Author (Tsana Makarim dika Nurulloh)

2	Biomedical Science	ABS-7
ISOLATION PURIFICATION AND CHARACTERIZATION BETA 2-MICROGLOBULIN IN HUMANS Yudith Yunia K(a*), Khomaini Hasan(b*), Olsha Fatya Clarista(c*) , Dewi Ratih H(d*), Harun Heri Trismiyanto (e*), Bernadetta Hayudewi(f*), Ludmilla Budikusuma(g*) (a*) Internal Medicine Department, (b,d*) Biochemistry Department, (c*) Bachelor Degree, (e,f*) Radiology Department, (g*) Pediatric Department of Medical Faculty of Universitas Jenderal Achmad Yani, Cimahi. Indonesia Abstract Introduction: Chronic kidney failure has long been a dangerous noncommunicable disease, reducing quality of life and raising treatment expenses. B2M is a low-molecular-weight protein present on the surface of all nucleated cells. The kidneys excrete 95% B2M, which is almost fully absorbed by the proximal tubule before being metabolized into amino acids. B2M is a protein discovered in chronic kidney disease that is rising in individuals on hemodialysis with traditional dialyzers. These individuals^ elevated B2M levels often correlate with increased chronic inflammation, which leads to elevated cardiovascular incidence, which correlates with elevated amyloidosis. The purpose of this study is to determine how to isolate, purify, and characterize B2M from blood samples from chronic kidney failure patients undergoing routine hemodialysis. Methods: This is a preliminary study to investigate the use of B2M protein. Protein isolation from blood serum extract was used, followed by protein isolation using ion exchange chromatography, SDS-PAGE electrophoresis, and finally protein characterization by detecting the molecular weight of the formed band. Result : Because a single band was not formed and the molecular characteristics ranging between 11 and 12.2 kDa in this protein, the results of this study were unable to find pure B2M protein. Conclusion : To obtain truly pure B2M, it is necessary to optimizing purification with other methods. Keywords: Beta 2-microglobulin (B2M)-Chronic Renal Disease (CKD)-Characterization- Isolation- Ion exchange chromatography Share Link | Plain Format | Corresponding Author (Dewi Ratih Handayani, M.Kes)

3	Biomedical Science	ABS-11
Charactertistics Based On Risk Factors, RMI2 Score, and Histopathology of Ovarian Cancer at RSHS Bandung Endah Hamidah Abbas (a), Ifa Siti Fasihah (b), Amelia Laili (c), Teja Koswara (a) a) Anatomical Pathology Departement, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi. Indonesia b) Obstetrics and Gynecology Departement, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi. Indonesia c) Medical Bachelor Study Program, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi. Indonesia Abstract Ovarian cancer is a malignant tumor of the ovaries in the form of primary malignancy derived from the ovary or secondary malignancies that come from metastases from other organs except ovary. Ovarian cancer is often diagnosed at an advanced stage, so ovarian cancer is known as ^The silent killer^, so the incidence and mortality rate of ovarian cancer in Indonesia is still high because of late diagnosis in the early stages. The purpose of this study was to find out the characteristics of ovarian cancer based on age, parity, history of contraception, RMI score 2, clinical stage, and diagnosis of histopathology at Dr. Hasan Sadikin Hospital Bandung in 2019-2020. This study is a descriptive study of total sampling method using medical record data of ovarian cancer patients in 2019-2020 at medical record center of Dr. Hasan Sadikin Hospital Bandung. The results of the study showed that 96 cases include criteria, the highest number of cases of ovarian cancer was in 2019 around 64.6%, the highest cases occurred in women aged > 50 years with 42.7%, multipara total 40.6%, and women who did not use contraception with 70.8%. About 71.4% patients had a total score of RMI 2 &#8805- 200 as malignant predictions. The majority of ovarian cancers are diagnosed at advanced stages, namely stage IIIB and IIIC with 20.3% each. Mucinous carcinoma is the most common histopathology type found at 20.4%. The incidence of ovarian cancer increases at the older age. Aging allows an extended period to accumulate genetic alterations within the ovarian surface epithelium. Keywords: Ovarian cancer, Dr. Hasan Sadikin Hospital, RMI 2 Score Share Link | Plain Format | Corresponding Author (Amelia Laili)

4	Biomedical Science	ABS-20
OVERVIEW OF HEPATOCELLULAR CARCINOMA PATIENTS IN CENTRAL GENERAL HOSPITAL DR. HASAN SADIKIN BANDUNG 2018-2020 Endah Hamidah Abbas (a*), Ilma Fiddiyanti (b), Ashria Tiara Agustina (c), Ris Kristiana (a) a) Pathology Anatomy Departement, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia *endah.hamidah123[at]yahoo.co.id b) Radiology Departement, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia c) Bachelor of Medicine Study Program, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Abstract Hepatocellular carcinoma is malignancy originates from hepatocyte cells. This cancer was the 3rd leading cause death worldwide due to cancer in 2020. Many patients with hepatocellular carcinoma are diagnosed at late stage where a poor prognosis. The purpose of this study was to describe hepatocellular carcinoma patients at Central General Hospital Dr. Hasan Sadikin Bandung. This study uses descriptive method and sample was taken from medical record of patients diagnosed with hepatocellular carcinoma from Medical Record Center of Hasan Sadikin Hospital 2018-2020 period with total sampling technique. The results showed that 156 patients were diagnosed with hepatocellular carcinoma with 69 patient meets our inclusion criteria. The most risk factor was liver cirrhosis with 27 patients (39,1%). 56-65 years age group was the most diagnosed with 21 patients (30.4%). Men were found more often than women with ratio 3:1. The most common clinical manifestations were abdominal pain and lethargy in 20 patients (29.0%). Stage D according to BCLC was the most common stage found with 26 patients (37.7%). The conclusions are liver cirrhosis was the most common risk factor of hepatocellular carcinoma. Most suffered in old age, male sex, and most common clinical manifestations are abdominal pain and lethargy with the most common stage according to BCLC was stage D. In liver cirrhosis, activated liver stellate cell causing continuous hepatocyte cell proliferation which the most common risk factor in this study. Hepatocellular carcinoma mostly occurs in men due to androgen receptor binding to EZH2 which inhibits p-53 gene. Keywords: BCLC, hepatocellular carcinoma, hepatocyte Share Link | Plain Format | Corresponding Author (Ashria Tiara Agustina)

5	Biomedical Science	ABS-30
ANTIBACTERIAL ACTIVITY OF SINGLE GARLIC AGAINST Salmonella typhi LIPOPOLYSACARIDE INVIVO IN Mus musculus Lia Siti Halimah1, Luthfi Nurlela2, Eka Noneng Nawangsih3, Reni Farenia4,Dhisa Ridzkianursepta5 1,3)Dept of Microbiology, Faculty of Medicine, Universitas Jenderal Achmad Yani 2)Dept of Parasitology, Faculty of Medicine, Universitas Jenderal Achmad Yani 4)Dept of Physiology, Faculty of Medicine, Universitas Jenderal Achmad Yani 5) Medical Study Program, Faculty of Medicine, Universitas Jenderal Achmad Yani Cimahi, Indonesia Abstract Black garlic is a fermented product of garlic. According to previous studies, black garlic has antibacterial activity against Salmonella typhi. S. typhi has lipopolysaccharide (LPS) which will activate inflammatory cytokine mediators such as TNF alpha. Tumor necrosis factor-alpha (TNF alpha) is one of the major cytokines in the acute inflammatory response. This study aimed to determine the antibacterial activity and concentration of single black garlic extract against S. typhi lipopolysaccharide in vivo in mice. This study used an experimental method with a post test only research design with control group using BALB/C white male mice which were divided into 5 groups, namely negative control (NC), positive control (PC), treatment 1 (T1) was given 25% extract. , treatment 2 (T2) was given 50% extract, and treatment 3 (T3) was given 75% extract. Lipopolysaccharide induction 0.5 mg/BW was carried out for 3 days after 14 days of extract administration. Blood samples were taken 24 hours after LPS induction. The average result of TNF alpha levels was obtained by the largest CP group with 8.43 pg/ml, the lowest NC group 4.69 pg/ml, of all treatment groups, the T3 group got the lowest 6.08 pg/ml. The results of data analysis using Kruskal Wallis showed p = 0.000 (p <0.05). This indicates that there are significant differences in TNF alpha levels in all study groups. It was concluded that a single black garlic extract had antibacterial activity because it could reduce the level of TNF alpha in lipopolysaccharide-induced mice with the most effective concentration of 75%. Keywords: Antibacterial, Black garlic, Lipopolysaccharide, TNF alpha Share Link | Plain Format | Corresponding Author (Lia Siti Halimah)

6	Biomedical Science	ABS-38
The Effect of Inulin and Sucrose Addition on The Number of Colonies L. acidophilus and B. bifidum In The Soyghurt After The Freeze-Drying Process Eka Noneng Nawangsih (a*), Apen Apgani (b), Bintari Rizkia Sekar Tirani (c) a) Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi, Indonesia. * eka.n.nawangsih[at]gmail.com b) Departemen Penyakit Dalam, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi, Indonesia. c) Program Studi Sarjana Kedokteran, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi, Indonesia. Abstract The freeze-dried soyghurt generally will be last much longer. However, the reduction of L. acidophilus and B. bifidum (LAB) colonies during the freeze-drying process could be occured and it could be minimized by adding inulin as a prebiotic and sucrose as a cryoprotector. This research aimed to determine whether or not the colonies number in freeze-dried soyghurt with the SNI and to analyze the effect of inulin and sucrose addition to the growth of the LAB in the freeze-dried soyghurt. This experimental laboratory study with pretest-posttest design consists of 1 negative control group, 1 group of soyghurt, and 4 groups of soyghurt with the addition of 10% sucrose and 0,5%, 1%, 3%, 5% inulin. The bacterial growth was calculated using the TPC method. All data were tested using the Saphiro Wilk test, followed by the Kruskal Wallis and Mann Whitney Post Hoc test. The conclusion of this research was that the number of colonies in each experimental groups decreased after the freeze-dried process and only the group of soyghurt without any additions did not meet the standard of SNI. Statistical tests showed a significant distinction between groups, giving a conclusion that the addition of inulin and sucrose gave a better incretion of the LAB significantly. 10% sucrose and 3% inulin was the best combination which has 2,34 x 10^29 CFU/ml as the highest TPC. It means that the higher of sucrose and inulin addition to the soyghurt, does not guarantee the number of LAB colonies inside will also be higher. Keywords: B. bifidum- Inulin- L. acidophilus- Soyghurt- Sucrose. Share Link | Plain Format | Corresponding Author (Bintari Rizkia Sekar Tirani)

7	Biomedical Science	ABS-39
Acupuncture Anesthesia on Awake Craniotomy for Tumor Removal: A Case Report Arief Kurniawan (a*), Nur Pudyastuti Pratiwi (b) (a) Department of Anesthesia and Intensive Care, Jenderal Achmad Yani University, Indonesia (b) Department of Hospital Management, Jenderal Achmad Yani University, Indonesia Abstract Acupuncture anesthesia is a type of acupuncture-based anesthesia. Anesthesia is achieved by placing needles into certain spots on the body, and the surgery is carried out while the patient is fully aware that he or she is no longer in pain. This approach avoids anesthesia-induced physiological dysfunction and post-operative anesthetic side effects. Acupuncture anesthesia is being used for head, chest, abdominal surgery, and awake craniotomy in persons of all ages. The anesthesiologist^s job in awake craniotomy is to deliver appropriate sedation and analgesia in patients who are still conscious and cooperative during the surgery while maintaining breathing and hemodynamic stability. A 42-year-old man was diagnosed with an oligodendroglioma-like supratentorial tumor. He was referred for three weeks of vomiting and headaches. His ECG and thoracic pictures were normal in the lab, but his MSCT revealed a hypodense lesion in the left temporal with modest mass effects. On an awake craniotomy, tumor resection was performed under acupuncture anesthesia. Electroacupuncture induction takes 20 minutes with the use of supplementary medications such as midazolam, fentanyl, and lidocaine. On a compliant and comfortable patient, the surgery can be completed without difficulty. After being examined in the PACU for 30 minutes without discomfort or PONV, he was transferred to the ward. We came to the conclusion that our patient responded well to the acupuncture anesthetic treatment. Keywords: Awake craniotomy, acupuncture, oligodendroglioma Share Link | Plain Format | Corresponding Author (Arief Kurniawan)

8	Biomedical Science	ABS-41
Noise Induced Hearing Loss Among Furniture Factory Workers PT Chitose Cimahi Yanti Nurrokhmawati Department of Ear Nose Throat - Head and Neck Surgery, Faculty of Medicine, General Achmad Yani University / Dustira Hospital, Cimahi Abstract Noise Induced Hearing Loss (NIHL) is a quite common health problem among factory workers, including furniture manufacturing factories. Noises above the threshold intensity for a long time can conduct to hearing loss, either temporary or permanent. If this is not taken seriously, it conduct to auditory and non-auditory impacts. The purpose of the study was to determine the prevalence of noise-induced hearing loss, worker characteristics, length of work, PPE use habit and auditory/non-auditory symptoms among workers at PT Chitose in Cimahi, West Java. Subjects were 66 workers consisting of 62 men and 4 women who were selected by total sampling, with a cross-sectional descriptive research method. Anamnesis was performed by filling out a questionnaire, physical examination of the ear, and pure tone audiometry. The results showed that the prevalence of NIHL was found at 37 people (56%). Out of patients with NIHL the average age was 37.8 years, male gender 95%, the workplace was from production department 73%, the average length of work was 16.6 years, never used PPE 54%. The most perceived auditory complaints were tinnitus 54%, and the most non-auditory complaints were difficulty sleeping 19%. In conclusion, hearing loss found in PT Chitose Cimahi furniture factory workers is hearing loss caused by noise. Keywords: noise, hearing loss, workers, furniture factory Share Link | Plain Format | Corresponding Author (Yanti Nurrokhmawati)

9	Biomedical Science	ABS-42
THE OVERVIEW OF MICROBIOLOGICAL QUALITY OF THE UPSTREAM OF CIKAPUNDUNG RIVER BASED ON TOTAL COLIFORM AND Escherichia coli Eka Noneng Nawangsih(1), Dinyar Supiadi W(2), Muhammad Azka Fadhlan Qinthara(3), Ania Kurniawati(4) (1)Bagian Mikrobiologi, Fakultas Kedokteran Universitas Jenderal Achmad Yani (2)Bagian Patologi Klinik, Fakultas Kedokteran Universitas Jenderal Achmad Yani (3)Program Studi Sarjana Kedokteran, Fakultas Kedokteran, Universitas Jenderal Achmad Yani (4)Bagian Mikrobiologi, Fakultas Kedokteran Universitas Jenderal Achmad Yani Abstract Cikapundung River is one of many rivers which its existence used by Bandung citizens for many function. One of the function is main water source for Dago Pakar Water Treatment Plant which is located in Bantar Awi. The social activity as a cow farmer can contaminate the quality of Cikapundung River because of the disposal of the waste that didn^t go through the process. The purpose of this research was to determine the amount of Total Coliform Bacteria and Escherichia coli contained in the upstream of Cikapundung River and to adapt with the regulation of the minister of the health of the Republic of Indonesia Number 32 of 2017. This research is a descriptive study conducted at Microbiology Laboratory, Faculty of Medicine in Jenderal Achmad Yani University. The sample was collected from three spots: upstream, midstream and downstream on three times which are morning, daylight, and afternoon. The method used is Most Probable Number with three steps including Presumptive test using Lactose Broth, Confirmed Test using BGLB and Completed Test using EMBA. The presumptive test showed all samples are positive except for the first spot which is from the upstream as main water source. The confirmative test showed on second and third spot taken on morning, daylight and afternoon contain very high amount of coliform which is 1101 CFU/100 ml. On completed test, E. coli, Enterobacter and Klebsiella colonies were found. It can be concluded that microbiological quality of Cikapundung River is not yet fullfiled the standard. Keywords: Cikapundung River, coliform, most probable number Share Link | Plain Format | Corresponding Author (Muhammad Azka Fadhlan Qinthara)

10	Biomedical Science	ABS-43
POTENCY OF RED GUAVA FRUITGHURT ON TOTAL CHOLESTEROL LEVELS Ania Kurniawati Purwa Dewi(1), Haura Khoerunnisa Badriarti(2), Yanti Nurrokhmawati(3) 1)Department of Microbiology, Faculty of Medicine, Jenderal Achmad Yani University, 2)Department of General Medicine, Faculty of Medicine, General Achmad Yani University, 3)Department of Ear Nose Throat Health Sciences - Head and Neck Surgery, Faculty of Medicine, Jenderal Achmad Yani University / Dustira Hospital. Abstract High total blood cholesterol levels are a risk factor for atherosclerosis and coronary heart disease. Probiotics are one of the potential foods that if consumed regularly can lower blood cholesterol levels. Fruitghurt is a probiotic whose raw materials come from fruits. The purpose of this study was to determine the effect of giving red guava fruitghurt on total blood cholesterol levels in male Wistar strain rats induced by a high-fat diet. The research design used is an experimental laboratory. The research subjects used white rats (Rattus norvegicus) wistar strain which were divided into 5 treatment groups. Data analysis was performed by T-dependent test, one way ANOVA and post hoc tukey. Based on the results of the T-dependent test, it can be concluded that there is a significant effect of giving red guava fruitghurt to decrease total cholesterol levels. Lactic acid bacteria contained in fruitghurt can increase the secretion of bile salt hydolase enzymes which can increase bile acid excretion so that total blood cholesterol levels can be reduced. In addition, lactic acid bacteria can also produce dehydrogenated cholesterol cofactors which function to activate the cholesterol reductase enzyme, causing sterols to be unable to be absorbed by the intestine, so total cholesterol will decrease. This proves that red guava fruitghurt has an antihypercholesterolemic effect in rats induced by a high-fat diet. Keywords: Probiotics, Fruitghurt, total cholesterol Share Link | Plain Format | Corresponding Author (Ania Kurniawati Purwa Dewi)

11	Biomedical Science	ABS-47
SUBCHRONIC TOXICITY TEST OF PAPAYA SEED (Carica papaya L.)ETHANOLIC EXTRACT ON THE LEVELS OF SGOT (Serum Glutamic Oxaloacetic Transaminase) IN WISTAR RATS Maman Djamaludin(a), Desy Linasari(b)/Ajeng Pastika(b) a) Departemen Farmakologi, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi. Indonesia b) Departemen Ilmu Kesehatan Masyarakat, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi. Indonesia/Program Studi Sarjana Kedokteran, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi. Indonesia Abstract Pepaya (Carica pepaya L.) merupakan salah satu tanaman yang digunakan sebagai obat herbal. Dari beberapa penelitian menyebutkan bahwa biji pepaya dapat bermanfaat sebagai antikolesterol pada dislipidemia, antiokisidan, antikejang, dan antidiare karena kandungan fenol, flavonoid, saponin, dan tannin. Tujuan penelitian ini untuk mengetahui adanya toksisitas subkronis berdasarkan pemeriksaan kadar SGOT (Serum Glutamic Oxaloacetic Transaminase) dari plasma tikus. Penelitian ini merupakan studi eksperimental murni dengan rancangan Post Test Only Controlled Group Design. Sampel berupa 40 ekor tikus yang dibagi menjadi 4 kelompok yaitu kelompok kontrol (K) dan perlakuan (P). Kelompok K hanya diberi akuades dan pelet. Kelompok P1, P2, dan P3 adalah kelompok yang diberikan ekstrak etanol biji pepaya dengan dosis bertingkat 250 mg/kgBB, 500 mg/kgBB, dan 1000 mg/kgBB selama 28 hari. Hari ke-29 dilakukan terminasi, kemudian diambil bahan pemeriksaan berupa plasma darah tikus. Uji statistik yang digunakan yaitu one way ANOVA. Hasil uji one way ANOVA pemeriksaan kadar SGOT terdapat perbedaan yang bermakna pada tikus jantan (p=0,048) dan betina (p=0,032). Saran: Dilakukan pengamatan histopatologis dengan untuk menilai sel-sel hati ataupun ginjal tikus karena adanya suatu jejas atau kerusakan sel akibat senyawa kimia selain ditandai dengan lesi biokimiawi juga dapat memberikan gambaran perubahan struktur mikroskopik dan dilakukan uji toksisitas ekstrak etanol biji pepaya secara kronis untuk mendeteksi efek toksik yang muncul setelah pemberian sediaan uji secara berulang sampai seluruh umur hewan. Keywords: Biji pepaya-Uji Toksisitas-Subkronis-SGOT Share Link | Plain Format | Corresponding Author (Ajeng Pastika)

12	Biomedical Science	ABS-49
In Vivo Study, Effect Combination of Lactobacilus spp and Doxycycline to Eliminate of Vibrio cholerae Eka Noneng Nawangsih, Siska Telly Pratiwi, Dinyar Supiadi Wijaya, Rachmayanti Nur Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi Abstract Infection by Vibrio cholerae is a major public health problem confronting developing countries. One of possible solution for the problems could come from probiotic which have been reported to reduce diarrhea. Aim of the research to determine effect combination of Lactobacillus spp and Doxycycline to eliminate of Vibrio cholerae in vivo. This research was an experimental study. Twenty five male Swiss Webster mouse were divided into five groups: K1 (negative control), K2 (positive control), K3 (treated by Doxycycline), and K4 (treated by Lactobacillus spp) and K5 (treated by combination of Lactobacillus spp and Doxycycline). Mouses are infected by Vibrio cholera until symptom arise and than treated for 3 days. Interval between treated Lactobacillus spp and Doxycycline was an hour. Feces was cultured on TCBS (Thiosulfate-citrate-bile salts-sucrose) media and than the colonies were counted by Total Plate Count method (TPC). Test of One-Way Anova (&#945- < 0,05) and post-hoc test (&#945- = 0,05) was applied. LSD post hoc test showed there were significantly difference between the group K1, K2 and K4, but K3 dan K5 were not significant. Group which treated by combination of Lactobacillus spp and Doxycycline reduced number of Vibrio cholerae lowest than another groups but not significant than treated by Doxycycline only. Lactobacillus spp. could eliminate Vibrio cholerae because it changed intraluminal acidity, produced antimicrobial substance, competition to absorp nutrition and attachment at the intestinal enterocyte receptor. Need longer interval between treated Lactobacillus spp and Doxycyclin. One hour interval not enough for Lactobacilus spp to work in gastrointestinal tract. Keywords: in vivo, Lactobacillus spp, Vibrio cholerae Share Link | Plain Format | Corresponding Author (Eka Noneng Nawangsih)

13	Biomedical Science	ABS-52
The Effect of Inulin and Sucrose Addition on The Number of Colonies L. acidophilus and B. bifidum In The Soyghurt After The Freeze-Drying Process Eka Noneng Nawangsih, Apen Apgani, Bintari Rizkia Sekar Tirani Faculty of medicine, Universitas Jenderal Achmad Yani Abstract The freeze-dried soyghurt generally will be last much longer. However, the reduction in the number of L. acidophilus and B. bifidum (LAB) colonies during the freeze-drying process could be occured and it could be minimized by adding inulin as a prebiotic and sucrose as a cryoprotector as well as a prebiotic. This research aimed to determine whether or not the colonies number in freeze-dried soyghurt with the SNI and to analyze the effect of inulin and sucrose addition to the growth of L. acidophilus and B. bifidum in the freeze-dried soyghurt. This experimental laboratory study with pretest-posttest design consists of 1 negative control group, 1 group of soyghurt, and 4 groups of soyghurt with the addition of 10% sucrose and 0,5%, 1%, 3%, 5% inulin. The bacterial growth was calculated using the TPC method. All data were tested using the Saphiro Wilk test, followed by the Kruskal Wallis test and the Mann Whitney Post Hoc test. The conclusion of this research was that the number of colonies in each experimental groups decreased after the freeze-dried process and only the group of soyghurt without any additions didn^t meet the standard of SNI. Statistical tests showed a significant distinction between groups, giving a conclusion that the addition of inulin and sucrose to the freeze-dried soyghurt gave a better incretion of L. acidophilus and B. bifidum significantly. 10% sucrose and 3% inulin was the best combination which has 2,34 x 1029 CFU/ml as the highest TPC. It means that the higher of sucrose and inulin addition to the soyghurt, doesn^t guarantee that the number of LAB colonies inside will also be higher. Keywords: B. bifidum, Inulin, L. acidophilus, Soyghurt, Sucrose. Share Link | Plain Format | Corresponding Author (Eka Noneng Nawangsih)

14	Biomedical Science	ABS-54
THE EFFECT OF RED GUAVA FRUITGHURT ON REDUCTION OF BLOOD GLUCOSE LEVELS IN STREPTOZOTOCIN INDUCED RATS Ania Kurniawati Purwa Dewi (1) Rona Jauza Husniah Dewi (2) Endry Septiadi(3) (1)Department of Microbiology, Faculty of Medicine, Jenderal Achmad Yani University, (2)Department of General Medicine, Faculty of Medicine, Jenderal Achmad Yani University, (3)Department of Nutrition, Faculty of Medicine, Jenderal Achmad Yani University. Abstract Diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose levels due to decreased insulin production, insulin action or both. Red guava fruitghurt contains flavonoid phytochemical compound. This research aims to determine the effect of red guava fruitghurt on reducing blood glucose levels in rats. The research design used was laboratory experiment with an experiment approach. The subjects of this study were white rats (Rattus norvegicus) with wistar lines, where each group needed 5 rats, therefore the total was 25 rats. The sampling technique used is simple random sampling, The experimental animals are grouped into 5 groups, namely KN, KP, P1, P2, and P3. The data analysis was performed with paired t test, one way anova, pairwise comparison test and post hoc duncan. Based on the results of the paired t test, it can be concluded that the significant effect in reducing blood glucose levels is the P3 (the rats induced by streptozotocin, given glibenclamide, and given fruitghurt). The decrease in blood glucose level is probably caused by the mechanism of glibenclamide and fruitghurt. Glibenclamide will stimulate pancreatic &#946- cells to increase insulin secretion. Giving fruitghurt can reduce blood glucose levels due to the mechanism of action of probiotics toward the body^s defences by strengthening the E-cadherin/Beta-cetanin complex so that it can improve intestinal permeability and the role of red guava which has a high content of flavonoids and total phenolic that works as a potent GLUT2 and SGLT1 inhibitor both in vitro and in vivo. Keywords: Fruitghurt, Red Guava, Blood Glucose Levels, Rats, Streptozotocin Share Link | Plain Format | Corresponding Author (Ania Kurniawati Purwa Dewi)

15	Biomedical Science	ABS-55
DIFFERENCE ROM (Range of Motion) ANKLE JOINTS IN USERS FLAT SHOES AND HIGH HEELS Hindun Saadah, Astri Pradini,Farhan Walid Department of Anatomy, Faculty of Medicine, University of Jenderal Achmad Yani Department of Histology Faculty of Medicine,University of Jenderal Achmad Yani Abstract High heels are one of the choices of women in supporting appearance, in addition, some agencies also require female employees to wear high heels. But in high heels users the position of the feet is constantly in a plantarflele position so that the muscles of the lower limbs that serve to move the ankle joint experience fatigue that can result in changes from the ROM of the ankle joint. This study aims to find out the difference in ROM in the ankle joints of users of flat shoes and high heels. The study used comparative analytical methods with a sample number of 44 respondents meeting inclusion criteria and the data obtained analyzed with the Mann Whitney test. The results of joint ROM analysis on dorsofleksi movement were obtained on average in flat shoes users 19.64 degrees and on high heels 13.09 degrees with a value of p<0.001. In plantarfleksi movements are obtained on average in flat shoes users 42.77 degrees and on high heels 65.09 degrees with a value of p<0.001. In high heels users, the muscles of the lower limbs that move the ankle joints are shortening and experiencing fatigue so that there can be changes in ankle joint ROM in high heels users. The conclusion of this study showed a significant difference in ankle joint ROM in users of high heels and flat shoes with a value of p<0.05. Keywords: ankle joints , dorsofleksi ,high heels, plantarfleksi, range of motion. Share Link | Plain Format | Corresponding Author (Hindun Saadah)

16	Biomedical Science	ABS-56
Luka Bakar, Kausa, Penatalaksanaan Dan Mencegah Gejala Sisa Iwan Darmawan Ma^mur Departemen Bedah Fakultas Kedokteran Universitas Jenderal Achmad Yani-Rumah Sakit Dustira Cimahi, Jawa Barat, Indonesia Abstract Luka bakar adalah suatu bentuk kerusakan jaringan yang disebabkan kontak dengan sumbar yang mempunyai suhu tinggi. Menurut WHO, tahun 2018 terdapat hampir 180.000 orang setiap tahun di dunia meninggal karena luka bakar. Luka bakar yang tidak fatal dapat mengarah kekematian. Dan tempat terjadinya mayoritas adalah di rumah dan tempat kerja. Dan luka bakar masih merupakan penyebab utama kecacatan di negara negara dengan penghasilan menengah ke bawah. Penyebab luka bakar diklasifikasikan menjadi 6 golongan mekanisme yaitu, air panas, kontak dengan benda panas, api, kimia, listrik dan radiasi. Angka survival dari luka bakar sangat tergantung dari 3 hal yaitu, usia, luas luka bakar dan ada atau tidaknya trauma inhalasi. Pasien dengan derajat luka bakar berat masih dikhawatirkan keselamatannya dengan timbulnya infeksi pada luka bakar. Penanganan pasien luka bakar harus dimulai dengan primary survey cepat prehospital. Jalan nafas harus menjadi perhatian utama. Intubasi awal akan menyelamatkan pasien bila terdapat trauma inhalasi. Secondary survey dilakukan meliputi mekanisme kejadian, penilaian Kembali adanya trauma inhalasi, intoxicasi CO dan assessment yang teliti terhadap luka bakar. Penanganan nyeri pada pasien luka bakar sangat krusial, penutupan luka dengan berbagai jenis bahan, pemberian antibiotic sampai tindakan pembedahan bila diperlukan. Timbulnya hipertropik scars dan keloid merupakan gejala sisa yang timbul kemudian. Dan akan membuat kecacatan permanen atau sementara. Penanganan sejak awal agar tidak timbulnya kecacatan permanen harus dilakukan sejak dini. Keywords: luka bakar, kausa, penata laksanaan, gejala sisa Share Link | Plain Format | Corresponding Author (Iwan Darmawan Mamur)

17	Biomedical Science	ABS-59
THE DIFFERENCES IN THE LEVEL OF ADHERENCE TO THE MEDITERRANEAN DIET ON LIPID PROFILE LEVEL AND PLASMA ATHEROGENIC INDEX IN DYSLIPIDEMIA PATIENTS Endry Septiadi (a*), Dewi Ratih Handayani (b), Khomaini Hasan (b), Iis Inayati Rakhmat (b), Yudith Yunia Kusmala (c), Rifky Atha^ullah Nuralif (d), Zacky Muttaqien Putra Nandita (d) (a) Departemen Ilmu Kesehatan Masyarakat, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia *endry.septiadi[at]lecture.unjani.ac.id (b) Departemen Biokimia, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia (c) Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia (d) Program Studi Sarjana Kedokteran, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia Abstract Dyslipidemia defined as increase in total cholesterol, triglycerides, Low Density Lipoprotein (LDL) and decrease in High Density Lipoprotein (HDL). The prevalence of Riset Kesehatan Dasar in 2018 showed 12.6% Indonesian population aged 55-64 years had high cholesterol and very high LDL and triglycerides. The Mediterranean diet is a non-pharmacotherapeutic treatments that recommended by American Heart Association for dyslipidemia. This research aims to determine differences of adherence to the Mediterranean diet on lipid profile and Plasma Atherogenic Index (PAI) in dyslipidemia patients. The research design used an observational analytic method with a prospective cohort of 30 dyslipidemia patients. Sampling was done by systematic random sampling technique. The subjects were divided into 3 categories based on adherence, namely low, medium and high, as measured by the alternate Mediterranean diet (aMED) score after filling the food frequency questionnaire four times in one month then lipid profile examination and PAI were assessed at the end of the month. Total cholesterol, LDL, HDL data were analyzed using Anova and Post Hoc Tukey whereas triglycerides and PAI were analyzed using Kruskal Wallis and Mann Whitney. Adherence to the Mediterranean diet showed significant results on total cholesterol (p=0.007), LDL level (p=0.019), HDL level (p=0.006), triglycerides (p=0.005) and PAI (p=0.036). Adherence to the Mediterranean diet has associated with lipid profile and PAI as a determinant of coronary heart disease in dyslipidemia patients due to the influence of food content rich in monounsaturated fatty acids, polyunsaturated fatty acids, phytosterols and polyphenols that affect lipid profiles and PAI. Keywords: Adherence, Lipid profile, Mediterranean diet, PAI Share Link | Plain Format | Corresponding Author (Endry Septiadi)

18	Biomedical Science	ABS-60
THE EFFECT OF BLACK RICE ETHANOL EXTRACT ON THE PROLIFERATION OF PMN WISTAR STRAINS RATS INDUCED ON LIPOPOLYSACCHARIDE Henny Juliastuti, Maman Djamaluddin, Hadi Alwani, Dewi Ratih Handayani Fakultas Kedokteran UNJANI, Cibeber, Kec. Cimahi Sel., Kota Cimahi, Jawa Barat 40531 Fakultas Kedokteran fk.unjani.ac.id Email korespondensi : juliastuti.henny[at]gmail.com Abstract Infectious diseases are a significant health problem in Indonesia. Inflammation is a defense in the body when an infection occurs. Lipopolysaccharides (LPS) are the most immunogenic components of a bacterial microbial cell wall that can cause an acute inflammatory response. Excessive inflammatory reactions will result in damaging and detrimental effects on the body. To overcome this, an anti inflammatory drug is needed to control the reaction so that it does not cause harm to the body. Black rice (Oryza sativa L.indica) is a natural ingredient that contains high levels of phenols, flavonoids, antioxidants, and immunomodulators. The target organ studied in this study is the spleen because it is an organ that removes antigens and innate and adaptive immune responses against pathogens. This study aims to determine the effect of giving black rice ethanol extract as an anti-inflammatory to the proliferation of PMN in the spleen glands of white rats induced by lipopolysaccharide. This study was conducted using 27 rats given black rice extract as much as 400 mg/KgBW for 14 days. Rats were induced with LPS 0.3 mg/KgBW at 24 hours intraperitoneally before CO2 euthanasia, and the spleen was taken to examine the size of the germinal center diameter. Observations showed the results of the diameter of the germinal center in the treatment group with a value of 1.311 +- 0.226 on the results of the Kruskal Wallis test, the value of p <0.05, meaning that there was a significant difference in the diameter of the germinal center between the treatment groups. The analysis results showed that the administration of LPS caused disorganization of the white pulp and reduced lymphocytes and PMN cells in the germinal center. In conclusion, black rice ethanol extract at a dose of 400 mg/kg BW can affect PMN proliferation as indicated by changes in the morphology of the white pulp in the germinal center in the spleen of rat. Keywords: Inflammation, Black rice, Proliferation, Lipopolysaccharid Share Link | Plain Format | Corresponding Author (HADI ALWANI)

19	Biomedical Science	ABS-67
CORRELATION BETWEEN PRESBYCUSIS AND QUALITY OF LIFE BASED ON HEARING HANDICAP INVENTORY FOR THE ELDERLY SCREENING VERSION (HHIE-S) Nurbaiti Nazarudin (a*), Vania Nanda Priasty (b) a) Department of Ear Nose Throat-Head and Neck Surgery Faculty of Medicine Universitas Jenderal Achmad Yani-Rumah Sakit Dustira, Cimahi, Jawa Barat, Indonesia b) Faculty of Medicine Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia Abstract Presbycusis is an age-related hearing loss caused by a degeneration process which is a cumulative effect of various risk factors. The unpreparedness of the elderly to encounter these conditions will have an impact on the low achievement on their quality of life. Hearing Handicap Inventory for the Elderly-Screening version (HHIE-S) questionnaire is a questionnaire that can be used to measure quality of life in patients with hearing loss. This study was aimed to determine whether there is a relationship between presbycusis and the quality of life, using cross sectional analytical research methods. The data used in form of primary data is the result of filling in the guided questionnaire and the results of the audiogram, while secondary data is in the form of medical records of presbycusis patients at Dustira Hospital in the period January 2017-December 2018. Data were analyzed descriptively and by Chi Square Test. During the study period, 55 patients who met inclusion criteria were obtained and 61,8% of them were in the age group of 65-74 year and 52,7% were male. Other characteristics examined 58.2% of them was married, 76,4% had a history of chronic diseases, while for economic status there were similarities between moderate and low economic status (49.1%). The results showed that there was a strong and significant relationship between presbycusis and patients^s quality of life (p <0.001). More severe degree of hearing loss, more severe decrease in patients quality of life. Keywords: HHIE-S, presbycusis, quality of life Share Link | Plain Format | Corresponding Author (Nurbaiti Nazarudin)

20	Biomedical Science	ABS-68
TINNITUS: CAUSES, DIAGNOSIS AND MANAGEMENT Nurbaiti Nazarudin Department of Ear Nose Throat-Head and Neck Surgery Faculty of Medicine Universitas Jenderal Achmad Yani-Rumah Sakit Dustira, Cimahi, Jawa Barat, Indonesia Abstract Tinnitus is the perception of sound that has no external source. The sensation is commonly describe as ringing in the ear, but the sound can be perceived inside or outside the head or predominanly in one or both ears. Prevalence of tinnitus of 10-25 % among persons older than 18 years of age across various nationalities. The prevalence tinnitus increases with age. Tinnitus is categorized as primary and secondary. Primary tinnitus more common and it is idiopathic, and most cases are associated with sensorineural hearing loss. Secondary tinnitus results from sound generated by a source near the ear or referred to the ear, and is rare, accounting for less than 1%. Secondary tinnitus has an identifiable cause, such as infectious, metabolic, neurologic, otologic, somatic, toxicologic, traumatic and vascular. Comprehensive audiologic evaluation should be performed for patients who report hearing difficulties, persistent tinnitus for more than 6 months, or unilateral tinnitus. The results of these evaluation will determine additional audiometric tests or diagnostic imaging. Treatments o reduce awareness of tinnitus and tinnitus-related distress include cognitive behavioral therapy, acoustic stimulaion and educational counseling. No medications, supplements, or herbal remedies have been shown to substantially reduce the severity of tinnitus. Keywords: causes, diagnosis, management, tinnitus Share Link | Plain Format | Corresponding Author (Nurbaiti Nazarudin)

21	Biomedical Science	ABS-70
HYPOCHOLESTEROLEMIC AND HYPOGLYCEMIC EFFECTS OF SOURSOP FRUIT (Annona muricata) ETHANOLIC EXTRACT IN HIGH FAT DIET AND ALLOXAN INDUCED WISTAR RATS Evi Sovia (a*), Nurbaiti Nazarudin(b), Ilma Fiddiyanti(c), Dinyar Supriyadi(d), Wini Fatimah Junaidi(e),Hidayah Dwijayanti(e), Helmi Makarim(e), Aprilia Grace Sweetasari(f) a)Pharmacology Department, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia *evi.sovia[at]lecture.unjani.ac.id b)Ear Nose and Throat Disease Department, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia c)Radiology Department, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia d)Clinical Pathology Department, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia e)Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia f)Neurologi Department, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia Abstract Annona muricata (soursop) is a plant belonging to the Annonaceae family. It^s a medicinal herb that^s been used for centuries as a natural cure for a number of diseases. Several investigations in animals showed that the bark and leaves of the plant possessed antihypertensive, vasodilator, anti-spasmodic, and cardio depressive. However, research on soursop fruit is still limited. This study aims to investigate the effect of soursop fruit ethanolic extract on blood glucose, total and Low Density Lipoprotein (LDL) cholesterol levels in high fat diet and alloxan-induced rats (Rattus novergicus). The study was done experimentally with pre and posttest control group design using 30 rats divided into 6 groups consisted of, normal group with standard diet, and five groups induced by high fat diet and alloxan then given 0.9 mg/kg of simvastatin, 0.5 mg/kg glibenclamide, 250 and 500mg/kg of soursop fruit ethanolic extract (SFEE), and one positive control group without treatment. Blood glucose, total and LDL cholesterol levels measurement was conducted after 21 days of treatment. Data was analyzed using dependent t-test and ANOVA . Results shows significant reduction in blood glucose, total and LDL cholesterol level within treatment groups (p<0.05). Futhermore, 500mg/kg SFEE has roughly the same effectiveness as 0.9 mg/kg of simvastatin in reducing the total cholesterol level (p > 0.05), but not for LDL cholesterol. Meanwhile, the effectiveness of 250 and 500 mg/kg SFEE in lowering blood glucose levels is the same as glibenclamide (p>0.05). In conclusion, SFEE shows hypoglycemic and hypocholesterolemic effect on both dosages. Keywords: annona muricata, hypoglicemic, hypocholesterolemic Share Link | Plain Format | Corresponding Author (Evi Sovia)

22	Biomedical Science	ABS-73
The Efectiveness Of Green Tea Polyphenols In Minimalizing Mercury-Induced Rat Glomerular Basal Membrane Damage Astri Pradini (a), H. Maman Djamaludin (b), Andhika Satriya Wicaksono (c), Linlin Haeni (a) a) Departemen Histologi, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia *pradini3[at]gmail.com b) Departemen Farmakologi, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia c) Program Studi Kedokteran, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi,Jawa Barat, Indonesia Abstract Green tea polyphenols are the main element in green tea with high antioxidant activities that considered as a first line of defense against free radicals in the body which can be caused by mercury intoxication. Mercury is a dangerous heavy metal against human body. Mercury can absorbed and damaged many vital organs, including kidney. Mercury-induced membranous nephropathy is a disease within kidney which manifests as a thickening of glomerular basal membrane. The purpose of this study is to determine the efectiveness of green tea polyphenols for minimalizing glomerular basal membrane damage causes by mercury in rat. Study used experimental post-test only control group design against 24 paraffin blocks of rat kidney which divided into five groups. The data was taken in the form of glomerular basal membrane area. Data analysis was done with a nonparametric test Kruskal-Wallis because the data is not distributed normally and then analysis is continued with a post hoc Mann-Whitney test. The average area of glomerular basal membrane in each groups were KN 204,727, KP 410,741, P50 376,991, P100 296,572 and P200 207,552. Kruskal Wallis test found a significant difference among the five groups). Meanwhile post hoc test found a significant difference between P100 and positive control group. Antioxidant activites within green tea polyphenols has statistically proven to minimalize basal membrane damage in rat kidney and 100 mg kg of green tea polyphenols were the most effective dose. Keywords: Green tea polyphenols, glomerular basal membrane damage, mercury Share Link | Plain Format | Corresponding Author (ASTRI PRADINI M.Si)

23	Biomedical Science	ABS-84
Viability of Lactobacillus acidophilus in soymilk soygurt in rat gastric Sayu Putu Yuni Paryati (a*), Seno Wibisono (b), Khomaini Hasan (c) a). Department of Microbiology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia b). Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia c). Department of Biochemistry, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia Abstract Due to harsh environment that must be passed in digestive system, one of important characteristic of lactic acid bacteria is acid tolerance which leads an ability to survive in the gastric. In the current study, rat gastric was used to evaluate a viability and a survival of Lactobacillus acidophilus in soybean based soygurt. Preliminary in vitro study suggested that the growth of L. acidophilus was pH dependent. The soygurt containing 17.8 log10 CFU/mL of L. acidophilus was administered orally to the rats per day. After 7 days consecutive administration, the viability of L. acidophilus in gastric juice of rats was assessed. The protein profile and the presence of lactic acid in the gastric juice were analyzed. The result showed that 9.2 log10 CFU/mL of L. acidophilus was survived to pass in gastric. The pH of the gastric juice was more acidic in the presence of lactic acid after soygurt administration compared to control. The protein profile of juice contained specific protein with molecular mass about 100, 50, 37 kDa and small peptide which probably secreted as bacterial responses against gastric environment. These findings suggested that L. acidophilus in soygurt is viable and survived in gastric environment and this soygurt is qualified as an alternative probiotic for human, especially with lactose intolerance. Keywords: Lactobacillus acidophilus, lactic acid, soygurt Share Link | Plain Format | Corresponding Author (Sayu Putu Yuni Paryati)

24	Biomedical Science	ABS-85
THE EFFECT OF PLUM (Prunus salicina Lindl) FRUIT FLESH ETHANOL EXTRACT ON MDA LEVELS OF MALE WISTAR RATS INDUCED BY HIGH FAT DIET Hendri Priyadi (a*), Indarti Trimurtini (a**), Fransiska Ambarukmi Pontjosudargo (a***) a) Faculty Of Medicine, Jenderal Achmad Yani University *henpri74.hp[at]gmail.com **indar_asoparie[at]yahoo.com ***ambarukmifransiska[at]gmail.com Abstract This study was conducted to assess the effect of Plum extract on liver damage with indicators of MDA levels in male rats. The target of this study was to obtain information on the benefits of natural ingredients as hepatoprotection in rats induced by a high-fat diet. A high-fat diet is expected to produce a state of fatty liver by increasing the liver MDA. This research is a laboratory study with a completely randomized design, using 25 samples which were grouped into 5 groups consisting of 5 rats, which are a negative group, a positive group, and three treatment groups who were given ethanol extract of plum dose of 0,8 mg/kg BW/day, 1,6 mg/kg BW/day and 2,4 mg/kg BW/day. The negative group was only given standard pellets while the rest were given a high-fat diet and induced by propylthiouracil. The indicator that will be assessed is the liver MDA. The data obtained were analyzed using analysis of variance (ANOVA) and further tests were carried out with Duncan^s test, with a 95% confidence degree. The results showed that the liver MDA levels in the group given Plum fruit extract at doses of 0.8 mg/kg BW and 1.6 mg/kg BW were significantly lower than the positive control group. Plum fruit extract was able to inhibit the increase in liver MDA levels at an effective dose of 0.8 mg/kg BW. Keywords: Antioxidants, High Fat Diet, Plum Extract, Liver MDA Share Link | Plain Format | Corresponding Author (Fransiska Ambarukmi Pontjosudargo)

25	Biomedical Science	ABS-87
SUBCHRONIC TOXICITY TEST OF PAPAYA SEED (Carica papaya L.)ETHANOLIC EXTRACT ON THE LEVELS OF SGOT (Serum Glutamic Oxaloacetic Transaminase) IN WISTAR RATS Maman Djamaludin(a), Desy Linasari(b)/Ajeng Pastika(b) FK UNJANI Abstract Pepaya (Carica pepaya L.) merupakan salah satu tanaman yang digunakan sebagai obat herbal. Dari beberapa penelitian menyebutkan bahwa biji pepaya dapat bermanfaat sebagai antikolesterol pada dislipidemia, antiokisidan, antikejang, dan antidiare karena kandungan fenol, flavonoid, saponin, dan tannin. Tujuan penelitian ini untuk mengetahui adanya toksisitas subkronis berdasarkan pemeriksaan kadar SGOT (Serum Glutamic Oxaloacetic Transaminase) dari plasma tikus. Penelitian ini merupakan studi eksperimental murni dengan rancangan Post Test Only Controlled Group Design. Sampel berupa 40 ekor tikus yang dibagi menjadi 4 kelompok yaitu kelompok kontrol (K) dan perlakuan (P). Kelompok K hanya diberi akuades dan pelet. Kelompok P1, P2, dan P3 adalah kelompok yang diberikan ekstrak etanol biji pepaya dengan dosis bertingkat 250 mg/kgBB, 500 mg/kgBB, dan 1000 mg/kgBB selama 28 hari. Hari ke-29 dilakukan terminasi, kemudian diambil bahan pemeriksaan berupa plasma darah tikus. Uji statistik yang digunakan yaitu one way ANOVA. Hasil uji one way ANOVA pemeriksaan kadar SGOT terdapat perbedaan yang bermakna pada tikus jantan (p=0,048) dan betina (p=0,032). Saran: Dilakukan pengamatan histopatologis dengan untuk menilai sel-sel hati ataupun ginjal tikus karena adanya suatu jejas atau kerusakan sel akibat senyawa kimia selain ditandai dengan lesi biokimiawi juga dapat memberikan gambaran perubahan struktur mikroskopik dan dilakukan uji toksisitas ekstrak etanol biji pepaya secara kronis untuk mendeteksi efek toksik yang muncul setelah pemberian sediaan uji secara berulang sampai seluruh umur hewan. Keywords: Biji pepaya-Uji Toksisitas-Subkronis-SGOT Share Link | Plain Format | Corresponding Author (Desy Linasari)

26	Biomedical Science	ABS-88
THE EFFECT OF RAMBUTAN HONEY AND RUTIN ON DECREASE BLOOD GLUCOSE AND INCREASE STREPTOZOTOCIN-INDUCED RAT PLASMA INSULIN 1Iis Inayati Rakhmat,1,2Euis Reni Yuslianti, 3Welly Ratwita, 4Teja Koswara, 1Nurul Sofiana Mutiadewi 1Department of Biochemistry, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531 2Department of Oral Biology, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531 3Department of Pharmacology, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531 4Department of Pathology Anatomy, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531 Abstract Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia due to insulin work disorders, insulin secretion disorders, or both. Rambutan honey as a natural ingredient has been studied to reduce oxidative stress, protect pancreatic beta cells from damage, and can increase insulin secretion from pancreatic beta cells. Rutin as secondary antioxidants can increase insulin secretion, reduce free radical formation, and can stimulate pancreatic progenitor cells to form new cells. This study aims to determine the effect of rambutan honey on blood glucose and insulin levels in rats induced by streptozotocin compared to rutin. This research is a pure experimental study with a total sample of 24 experimental animals consisting of 4 groups, namely negative control (NC), positive control (PC), and 2 treatment groups (T1-T2). Glucose levels were measured using the GOD-PAP method and were analyzed statistically (T-dependent p <0.05, Anova, Duncan p <0.05). Insulin levels were measured using a mouse insulin ELISA-kit and were statistically analyzed (Anova, Duncan p <0.05). The results showed that rambutan honey and rutin could significantly reduce blood glucose levels (p = 0.008 and p = 0.010). Data on insulin levels showed significant differences between groups (p = 0.000). The conclusion of this study is that rambutan honey and rutin can reduce blood glucose level and increase insulin levels because they are antioxidant agents which have antihyperglycemic effects. Keywords: blood glucose, diabetes, insulin, rambutan honey, rutin Share Link | Plain Format | Corresponding Author (Iis Inayati Rakhmat)

27	Biomolecular and Bioinformatic	ABS-21
Identification of Bacteriocin Gene in Lactobacillus acidophilus Siska Telly Pratiwi (a*), Panca Andana (b) a) Departemen Mikrobiologi, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi. Indonesia *siska.telly[at]lecture.unjani.ac.id b)Program Studi Sarjana Kedokteran, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Cimahi. Indonesia Abstract Lactic acid bacteria have the ability to produce antimicrobial substances such as bacteriocins. They are of great interest to research due to some of the lengthy annals of safe use and the generally considered safe (GRAS) and presumed safety (QPS) status. Bacteriocins are small, hydrophobic, cationic peptides with bacteriostatic or bactericidal activity in various microbes other than the producing strain. The production of bacteriocin depends on its strain and culture optimization. Genes encoding bacteriocin are located in chromosomes or plasmids. This study was carried out to detect the bacteriocin gene in Lactobacillus acidophilus FNCC 0051 and ATCC 4356. Non-lactic acid bacteria (Bifidobacterium bifidum), nuclease-free water, and distilled water are used as the negative control. The DNA extraction was carried out following Agilent DNA Extraction Kit with some modifications. Primers used in this study are specific gene primers for acidocin LF221A, and the PCR cycling condition was done based on it. The result of using PCR showed that Lactobacillus acidophilus FNCC 0051 and ATCC 4356 have acidocin LF221A gene. It proved by electrophoresis analysis that the amplicon showed bands with a size less than 100 bp. The conclusion is that Lactobacillus acidophilus in this study contains gene encoding bacteriocin. Vice versa, non-lactic acid bacteria such as Bifidobacterium bifidum does not carry the bacteriocins gene. Keywords: acidocin, bacteriocin, DNA, gene, Lactobacillus acidophilus Share Link | Plain Format | Corresponding Author (Panca Andana)

28	Clinical Immunology	ABS-76
-Dimer/Fibrinogen Ratio In Confirming Pulmonary Embolism with Pulmonary Ct Angiography: A Case Series Dis Bima Purwaamidjaja, Juan Carson R.N. Marbun, Irada Imaniar, Zetto Arya Buana ICU Gatot Soebroto Presidential Army Hospital, Jakarta, Indonesia Abstract Introduction: A correct diagnosis of pulmonary embolism (PE) is essential because early initiation of appropriate therapy decreases mortality. Elevated D-dimer has long raised the susceptibility of PE but still has to undergo computed tomography (CT) angiography for confirmation. High D-dimer combined with low or normal fibrinogen levels, best expressed as D-dimer/fibrinogen (D/F) ratio might increase the diagnostic rate. Case: Three cases of three ICU patients of Gatot Soebroto Army Hospital with the moderate clinical probability of PE and D/F ratio > 0,23 x 10-3, all confirmed as PE with pulmonary CT angiography. Discussion: High D-dimer combined with low or normal fibrinogen levels, best expressed as D/F ratio might increase the diagnostic rate. It is not considered to replace approved imaging tests for PE diagnosis, however, it seems useful as an additional modality to raise the susceptibility and establish the diagnosis before confirmation with CT angiography. In this case series, the three patients above have different diagnoses but all three diagnoses have a correlation to an elevated D-dimer. Elevated D-dimer in these patients may not be used to predict PE. Some conditions that produce D-dimer lower its specificity in predicting PE. Conclusion: D/F ratio 0,23 x 10-3 is useful as an additional module to raise the susceptibility of PE and establish the diagnosis before confirmation with CT angiography. Keywords: D-dimer/fibrinogen ratio, pulmonary embolism, CT angiography Share Link | Plain Format | Corresponding Author (Dis Bima Purwaamidjaja)

29	Clinical Practice	ABS-5
Forgarty Catheter for Lung Isolation and One-lung Ventilation in Infants with Multiloculated Empyema with Bronchopleural Fistula Undergoing Exploratory Thoracotomy: an Alternative of Double Lumen Tube Baginda Aflah (a*), Iwan Abdul Rachman (b), Suwarman (b) a) Department of Anesthesiology and Intensive Therapy b) Faculty of Medicine, Padjadjaran University Abstract Abstract Lung isolation and one-lung ventilation are generally facilitated by the use of a Double Lumen Catheter (DLT) to achieve a good operating field, prevent contamination, maintain adequate ventilation and oxygenation. In the infant and pediatric population, it becomes a problem because there is no suitable size of DLT on the market. In this article, we report a 2.5-month old infant with multiloculated empyema with bronchopleural fistula in the right lung who underwent a thoracotomy for decortication and closure of the fistula. In this case, we used the Forgarty Catheter, commonly used in vascular surgery for thrombectomy, as an alternative to DLT. We made this Forgarty Catheter a bronchial blocker to isolate the patient^s right lung, we inserted it into a modified endotracheal tube (ETT) with a Y connector. Intraoperatively, the Forgarty catheter can properly act as a bronchial blocker to prevent contamination of healthy lungs, to facilitate one-lung ventilation and oxygenation can be provided adequately. Postoperatively the Forgarty catheter was removed and both of the patient^s lungs were re-expanded and the patient was transferred to the PICU for further observation. In conclusion, Forgarty Catheter can be an alternative to DLT in infants and pediatrics undergoing thoracic surgery. Keywords: Forgarty Catheter, lung isolation, One lung ventilation, Thoracic surgery, Pediatric, Empyema, Bronchopleural fistula. Share Link | Plain Format | Corresponding Author (Baginda Aflah)

30	Clinical Practice	ABS-8
THE SUITABILITY OF THE RESULTS OF CT-SCAN THORAX EXAMINATION WITH CLINICAL SYMPTOMS OF COVID-19 PATIENTS AT KARAWANG HOSPITAL IN 2020-2021 PERIOD Ilma Fiddiyanti (a*), Lukmana Lokarjana (b), Erika Mustika Rahayu (c) (a) Department of Radiology, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia *ilmaradiologi[at]gmail.com (b) Department of Surgery, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia (c) Doctoral Education Study Program, Faculty of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Abstract Coronavirus Disease 2019 (COVID-19) is a respiratory disease caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2). Anyone could have asymptomatic to severe symptoms. Radiological examinations, such as X-rays and CT-Scans, are needed. CT-Scan thorax is an examination to assess the progression, severity, and comorbidities based on the consensus of the Radiology Society of North America (RSNA). This study aims to find out the suitability of the results of CT-Scan thorax examination with clinical symptoms of COVID-19 patients at Karawang Hospital in 2020-2021 period. The study used a cross- sectional design with consecutive sampling method. The results of data collection obtained 19 patients, with 13 patients included in inclusion and exclusion criteria. Statistical test using kappa test. The results showed that criteria for age of 50-59 years were 6 people (46.2%) and mostly found in men (61.5%). Moderate-severe symptoms for clinical symptoms criteria in 8 people (61.5%) were found. The characteristics of thorax CT-Scan showed an image of GGO, consolidation, crazy paving pattern, a halo sign in 9 people (69.2%), and thickening of blood vessels in 5 people (38.5%). Based on consensus of RSNA, indeterminate-typical were found in 9 people (69.2%). The results of the kappa test analysis showed conformity with the value of k = 0.675. This research shows the suitability of the results of thorax CT-scan based on RSNA consensus with clinical symptoms of COVID-19. Keywords: Clinical symptoms, COVID-19, RSNA Share Link | Plain Format | Corresponding Author (Erika Mustika Rahayu)

Page 1 (data 1 to 30 of 84) | Displayed ini 30 data/page
1 2 3 NEXT >>

Powered By Konfrenzi Ultimate 1.832M-Build3 © 2007-2026 All Rights Reserved