THE EFFECT OF RAMBUTAN HONEY AND RUTIN ON DECREASE BLOOD GLUCOSE AND INCREASE STREPTOZOTOCIN-INDUCED RAT PLASMA INSULIN 1Iis Inayati Rakhmat,1,2Euis Reni Yuslianti, 3Welly Ratwita, 4Teja Koswara, 1Nurul Sofiana Mutiadewi
1Department of Biochemistry, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531
2Department of Oral Biology, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531
3Department of Pharmacology, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531
4Department of Pathology Anatomy, Faculty of Medicine, Universitas Jenderal Ahmad Yani, Cimahi, West Java, Indonesia 40531
Abstract
Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia due to insulin work disorders, insulin secretion disorders, or both. Rambutan honey as a natural ingredient has been studied to reduce oxidative stress, protect pancreatic beta cells from damage, and can increase insulin secretion from pancreatic beta cells. Rutin as secondary antioxidants can increase insulin secretion, reduce free radical formation, and can stimulate pancreatic progenitor cells to form new cells. This study aims to determine the effect of rambutan honey on blood glucose and insulin levels in rats induced by streptozotocin compared to rutin. This research is a pure experimental study with a total sample of 24 experimental animals consisting of 4 groups, namely negative control (NC), positive control (PC), and 2 treatment groups (T1-T2). Glucose levels were measured using the GOD-PAP method and were analyzed statistically (T-dependent p <0.05, Anova, Duncan p <0.05). Insulin levels were measured using a mouse insulin ELISA-kit and were statistically analyzed (Anova, Duncan p <0.05). The results showed that rambutan honey and rutin could significantly reduce blood glucose levels (p = 0.008 and p = 0.010). Data on insulin levels showed significant differences between groups (p = 0.000). The conclusion of this study is that rambutan honey and rutin can reduce blood glucose level and increase insulin levels because they are antioxidant agents which have antihyperglycemic effects.
