ANTIBACTERIAL ACTIVITY OF SINGLE GARLIC AGAINST Salmonella typhi LIPOPOLYSACARIDE INVIVO IN Mus musculus Lia Siti Halimah1, Luthfi Nurlela2, Eka Noneng Nawangsih3, Reni Farenia4,Dhisa Ridzkianursepta5
1,3)Dept of Microbiology, Faculty of Medicine, Universitas Jenderal Achmad Yani
2)Dept of Parasitology, Faculty of Medicine, Universitas Jenderal Achmad Yani
4)Dept of Physiology, Faculty of Medicine, Universitas Jenderal Achmad Yani
5) Medical Study Program, Faculty of Medicine, Universitas Jenderal Achmad Yani
Cimahi, Indonesia
Abstract
Black garlic is a fermented product of garlic. According to previous studies, black garlic has antibacterial activity against Salmonella typhi. S. typhi has lipopolysaccharide (LPS) which will activate inflammatory cytokine mediators such as TNF alpha. Tumor necrosis factor-alpha (TNF alpha) is one of the major cytokines in the acute inflammatory response. This study aimed to determine the antibacterial activity and concentration of single black garlic extract against S. typhi lipopolysaccharide in vivo in mice. This study used an experimental method with a post test only research design with control group using BALB/C white male mice which were divided into 5 groups, namely negative control (NC), positive control (PC), treatment 1 (T1) was given 25% extract. , treatment 2 (T2) was given 50% extract, and treatment 3 (T3) was given 75% extract. Lipopolysaccharide induction 0.5 mg/BW was carried out for 3 days after 14 days of extract administration. Blood samples were taken 24 hours after LPS induction. The average result of TNF alpha levels was obtained by the largest CP group with 8.43 pg/ml, the lowest NC group 4.69 pg/ml, of all treatment groups, the T3 group got the lowest 6.08 pg/ml. The results of data analysis using Kruskal Wallis showed p = 0.000 (p <0.05). This indicates that there are significant differences in TNF alpha levels in all study groups. It was concluded that a single black garlic extract had antibacterial activity because it could reduce the level of TNF alpha in lipopolysaccharide-induced mice with the most effective concentration of 75%.
Keywords: Antibacterial, Black garlic, Lipopolysaccharide, TNF alpha
