ISOLATION PURIFICATION AND CHARACTERIZATION BETA 2-MICROGLOBULIN IN HUMANS Yudith Yunia K(a*), Khomaini Hasan(b*), Olsha Fatya Clarista(c*) , Dewi Ratih H(d*), Harun Heri Trismiyanto (e*), Bernadetta Hayudewi(f*), Ludmilla Budikusuma(g*)
(a*) Internal Medicine Department,
(b,d*) Biochemistry Department,
(c*) Bachelor Degree,
(e,f*) Radiology Department,
(g*) Pediatric Department of Medical Faculty of Universitas Jenderal Achmad Yani, Cimahi. Indonesia
Abstract
Introduction: Chronic kidney failure has long been a dangerous noncommunicable disease, reducing quality of life and raising treatment expenses. B2M is a low-molecular-weight protein present on the surface of all nucleated cells. The kidneys excrete 95% B2M, which is almost fully absorbed by the proximal tubule before being metabolized into amino acids. B2M is a protein discovered in chronic kidney disease that is rising in individuals on hemodialysis with traditional dialyzers. These individuals^ elevated B2M levels often correlate with increased chronic inflammation, which leads to elevated cardiovascular incidence, which correlates with elevated amyloidosis. The purpose of this study is to determine how to isolate, purify, and characterize B2M from blood samples from chronic kidney failure patients undergoing routine hemodialysis.
Methods: This is a preliminary study to investigate the use of B2M protein. Protein isolation from blood serum extract was used, followed by protein isolation using ion exchange chromatography, SDS-PAGE electrophoresis, and finally protein characterization by detecting the molecular weight of the formed band.
Result : Because a single band was not formed and the molecular characteristics ranging between 11 and 12.2 kDa in this protein, the results of this study were unable to find pure B2M protein.
Conclusion : To obtain truly pure B2M, it is necessary to optimizing purification with other methods.
