THE EFFECT OF BLACK RICE ETHANOL EXTRACT ON THE PROLIFERATION OF PMN WISTAR STRAINS RATS INDUCED ON LIPOPOLYSACCHARIDE Henny Juliastuti, Maman Djamaluddin, Hadi Alwani, Dewi Ratih Handayani
Fakultas Kedokteran UNJANI, Cibeber, Kec. Cimahi Sel., Kota Cimahi, Jawa Barat 40531
Fakultas Kedokteran fk.unjani.ac.id
Infectious diseases are a significant health problem in Indonesia. Inflammation is a defense in the body when an infection occurs. Lipopolysaccharides (LPS) are the most immunogenic components of a bacterial microbial cell wall that can cause an acute inflammatory response. Excessive inflammatory reactions will result in damaging and detrimental effects on the body. To overcome this, an anti inflammatory drug is needed to control the reaction so that it does not cause harm to the body. Black rice (Oryza sativa L.indica) is a natural ingredient that contains high levels of phenols, flavonoids, antioxidants, and immunomodulators. The target organ studied in this study is the spleen because it is an organ that removes antigens and innate and adaptive immune responses against pathogens. This study aims to determine the effect of giving black rice ethanol extract as an anti-inflammatory to the proliferation of PMN in the spleen glands of white rats induced by lipopolysaccharide. This study was conducted using 27 rats given black rice extract as much as 400 mg/KgBW for 14 days. Rats were induced with LPS 0.3 mg/KgBW at 24 hours intraperitoneally before CO2 euthanasia, and the spleen was taken to examine the size of the germinal center diameter. Observations showed the results of the diameter of the germinal center in the treatment group with a value of 1.311 +- 0.226 on the results of the Kruskal Wallis test, the value of p <0.05, meaning that there was a significant difference in the diameter of the germinal center between the treatment groups. The analysis results showed that the administration of LPS caused disorganization of the white pulp and reduced lymphocytes and PMN cells in the germinal center. In conclusion, black rice ethanol extract at a dose of 400 mg/kg BW can affect PMN proliferation as indicated by changes in the morphology of the white pulp in the germinal center in the spleen of rat.
Keywords: Inflammation, Black rice, Proliferation, Lipopolysaccharid
