In Vivo Study, Effect Combination of Lactobacilus spp and Doxycycline to Eliminate of Vibrio cholerae
Eka Noneng Nawangsih, Siska Telly Pratiwi, Dinyar Supiadi Wijaya, Rachmayanti Nur

Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi

Abstract

Infection by Vibrio cholerae is a major public health problem confronting developing countries. One of possible solution for the problems could come from probiotic which have been reported to reduce diarrhea. Aim of the research to determine effect combination of Lactobacillus spp and Doxycycline to eliminate of Vibrio cholerae in vivo. This research was an experimental study. Twenty five male Swiss Webster mouse were divided into five groups: K1 (negative control), K2 (positive control), K3 (treated by Doxycycline), and K4 (treated by Lactobacillus spp) and K5 (treated by combination of Lactobacillus spp and Doxycycline). Mouses are infected by Vibrio cholera until symptom arise and than treated for 3 days. Interval between treated Lactobacillus spp and Doxycycline was an hour. Feces was cultured on TCBS (Thiosulfate-citrate-bile salts-sucrose) media and than the colonies were counted by Total Plate Count method (TPC). Test of One-Way Anova (&#945- < 0,05) and post-hoc test (&#945- = 0,05) was applied. LSD post hoc test showed there were significantly difference between the group K1, K2 and K4, but K3 dan K5 were not significant. Group which treated by combination of Lactobacillus spp and Doxycycline reduced number of Vibrio cholerae lowest than another groups but not significant than treated by Doxycycline only. Lactobacillus spp. could eliminate Vibrio cholerae because it changed intraluminal acidity, produced antimicrobial substance, competition to absorp nutrition and attachment at the intestinal enterocyte receptor. Need longer interval between treated Lactobacillus spp and Doxycyclin. One hour interval not enough for Lactobacilus spp to work in gastrointestinal tract.

Keywords: in vivo, Lactobacillus spp, Vibrio cholerae

Topic: Biomedical Science