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31	Animal Nutrition and Feed Technology	ABS-51
Role of Rumen Protected L-Arginine for Sheep Reproduction and Production (Literatur Review) Muhammad Fadhlirrahman Latief, Osfar Sofjan, Jasmal Ahmari Syamsu, Suyadi 1 Faculty of Animal Science, Brawijaya University 2 Center for Research and Development of Animal Science and Tropical Animal Resources (PUSLITBANG SENTRO) Hasanuddin University 3 Feed Technology and Industry Laboratory, Faculty of Science, Hasanuddin University Abstract L-arginine is an important amino acid for growth, reproduction, and production in livestock. However, arginine is susceptible to degradation in the rumen, which affects the availability of arginine for the animals. databases using the keywords ^Arginine^, ^Condensed Tannin^, ^Rumen Protected^, ^Sheep^, and ^Seminal Plasma^. Therefore, protecting arginine in the rumen is crucial to improve the efficiency of arginine utilization and animal performance. Several studies have shown that feeding protected arginine can enhance lamb growth and sperm quality in rams. The improvement in lamb growth and sperm quality may be due to the positive effects of arginine on protein synthesis and nitric oxide synthesis. Furthermore, feeding protected arginine can also improve feed efficiency and reduce feed costs in sheep production. However, some studies have also shown that feeding protected arginine does not always improve animal performance, especially when there is already sufficient arginine available in the diet. In conclusion, protecting arginine in the rumen can improve the efficiency of arginine utilization and animal performance, especially in terms of lamb growth and sperm quality in rams. However, careful consideration of the dose and timing of feeding protected arginine is required to achieve optimal results. Keywords: Arginine, Condensed Tannin, Rumen Protected, Sheep, Seminal Plasma Share Link | Plain Format | Corresponding Author (M. Fadhlirrahman Latief)

32	Animal Nutrition and Feed Technology	ABS-52
The Effect of Protease and NSP Enzyme Supplementation on Metabolizable Energy and Protein Digestibility of Jack Beans (Canavalia ensiformis L.) Raudhoh Gholia Sakinah(1), Muhammad Ridla(2,3*), Widya Hermana(2,3) 1) Study Program of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia 2) Department of Animal Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia *hmridla[at]apps.ipb.ac.id 3) Center For Tropical Animal Studies (CENTRAS), IPB University, Jl, Raya Pajajaran, Bogor, 16153 Abstract The objective of this study was to evaluate the physicochemical quality of whole Jack Beans mill and measure the Metabolic Energy (ME) and protein digestibility in broiler chickens by adding NSP and protease enzymes. The study used 20 five-week-old Cobb strain broiler chickens and followed a completely randomized design (CRD) with four treatments and four replicates. The treatments included P0 = Jack bean mill, P1 = P0 + Protease Enzyme, P2 = P0 + NSP Enzyme, and P3 = P0 + Protease Enzyme + NSP Enzyme. The results showed that adding NSP and protease enzymes to the feed increased the biological value, including metabolic energy and protein digestibility. The measurements of bulk density, tapped density, and metabolic energy in P1, P2, and P3 treatments were significantly (p<0.05) higher than in the P0 treatment. Based on the results, the study concludes that incorporating NSP and protease enzymes can enhance the nutritional value of jack beans for broiler chickens Keywords: protease enzyme, NSP enzyme, Jack beans, physicochemical properties, metabolic digestibility, broiler chickens Share Link | Plain Format | Corresponding Author (Muhammad Ridla)

33	Animal Nutrition and Feed Technology	ABS-54
Immune response (antibody titers and leukocyte profile) of local chickens given in ovo butyric acid Rantan Krisnan, Cecep Hidayat, Mohammad Ikhsan Shiddieqy, Endang Sutedi, Iwan Herdiawan, Tuti Haryati, Rusdiana Supardi, Yuli Retnani, M. Hamdan, Andi S Research Organization for Agriculture and Food, National Research and Innovation Agency Abstract The aim of this study was to determine the immune response of local chickens given butyric acid through the in ovo technique. There were five in ovo material treatments, namely- T1 (admix sodium butyrat), T2 (pure butyric acid), T3 (antibiotic), T4 (Phosphate-buffered saline / Sham), and T5 (uninjected). All ingredients were dissolved in 0.5 ml PBS every injection at a dose of 1 kg/ton butyric acid.The injection process is carried out on the amnion while 18 days of incubation using a 20G needle (diameter 0.9 mm and a length 8 mm). The parameters observed were antibody titers, total and differentiation of leukocytes. The results showed that butyric acid treatment and antibiotic treatment significantly (P<0.05) gave a better effect on antibody titers, lymphocytes and heterophils compared to sham and uninjected treatment. However, it had no significant effect (P>0.05) on total leukocytes and other leukocyte differentiation. There was no significant effect due to the use of different types of butyric acid, so the recommendation of the type of butyric acid chosen will depend on the consideration of economic aspects and its availability. Keywords: in ovo, butyric acid, antibody titer, leukocyte, local chicken. Share Link | Plain Format | Corresponding Author (Rantan Krinsan)

34	Animal Nutrition and Feed Technology	ABS-55
Comparison of Phytochemical Analysis of Turmeric Before Fermentation and After Fermentation and Recovery of Lactic Acid Bacteria as Phytogenic Feed Additives (PFA) Kasri1b) Lilik Eka Radiati1), Osfar Sofjan 1a) 1Faculty of Animal Science, Brawijaya University Abstract This study aimed to compare the phytochemical content of turmeric before and after fermentation, as well as the recovery of lactic acid bacteria (LAB) as a phytogenic feed additive (PFA). The method used in this study was to conduct a literature search through PubMed, ScienceDirect, and Google Scholar databases using the keywords : turmeric fermentation, phytochemicals, lactic acid bacteria, and phytogenic feed additives. The results of the literature review showed that turmeric fermentation can increase the content of phenolic compounds, flavonoids, and tannins. In addition, fermentation also increases the antioxidant activity of turmeric. After fermentation, turmeric also contains higher amino acid and fatty acid compounds than before fermentation. The turmeric fermentation process can be carried out using probiotic microbial starters, such as lactic acid bacteria from the genus Lactobacillus and Enterococcus. LAB recovery from fermented turmeric can be done by several methods, such as serial dilution, salt dilution, and filtration. The recovered LAB can be used as PFA in animal feed, as it can improve animal health through improved nutritional quality and increased immunity. In conclusion, this literature review shows that turmeric fermentation can increase phytochemical content and antioxidant activity, and recovered LAB can be used as PFA to improve animal feed quality and animal health. Further research is needed to evaluate the effectiveness and safety of using fermented turmeric and recovered LAB as PFA in animal feed. Keywords: Turmeric Fermentation, Phytochemicals, Lactic Acid Bacteria, Phytogenic Feed Additive Share Link | Plain Format | Corresponding Author (Kasri Kasri)

35	Animal Nutrition and Feed Technology	ABS-59
Effect of Grass Substitution using Sugarcane Tops Treated with Cytophaga sp on In Vitro Rumen Fermentation Agustin Herliatika1, a), Wisri Puastuti1, b), Dwi Yulistiani1, c), Susan IW Rakhmani1, d), Winwin Widaringsih1, e), Maplani1, f) 1 Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency, Indonesia. Cibinong Sciences Center, Bogor, Indonesia, 16915 2Additional affiliations should Abstract Processed sugarcane tops could be used as ruminant feed to substitute grass. This study aims to evaluate the in vitro rumen fermentability of sugarcane top (SCT) fermented with Cytophaga sp (SCTC) in the ration. The fermentation process of the Sugarcane top was conducted for five days. An in vitro test was carried out to measure sugarcane top fermentability in complete rations as a grass substitute. The rations were formulated using forage and concentrate at a ratio of 1:1. Elephant Grass as basal forage was substituted using fermented sugarcane top (SCTC) at 0, 20, 40, and 50% DM of forage. Substitution of 50% unprocessed SCT in the ratio was also evaluated as control (+). This experiment was conducted in a completely randomized design with five treatments and five replications. Results showed that the substitution of SCTC affected the diet NH3 levels, dry matter digestibility, bacteria, and protozoa population (P<0.05), but it did not affect total VFA (P>0.05). The dry matter digestibility of SCT or SCTC rations has a higher trend compared to the control (61.54-66.19% vs 58.39%). Methane gas production of all treatments was similar (44.4-48.9 ml/g DM digested). This study concluded that SCT and SCTC could be used as grass substitutes in the ration without interfering with the rumen fermentation. Keywords: sugarcane tops, Cytophaga sp, fermentation, rumen Share Link | Plain Format | Corresponding Author (Agustin Herliatika)

36	Animal Nutrition and Feed Technology	ABS-61
The Growth Rate of Weaner Madura Bulls Fed Organic Zinc in the Concentrate Feed and Elephant Grass Risa Antari1, Alif Shabira Putri1, Angga Maulana Firmansyah1, Wahyuni Indah Wulansari1, Yenny Nur Anggraeny1, Noor Hudhia Krishna1, Hartati1, and Mariyono1 1National Research and Innovation Agency, Cibinong, Kabupaten Bogor 16915, West Java-Indonesia. Abstract Zinc-lysin is an organic mineral that is essential. This research aimed to examine the use of organic-sourced zinc to improve the growth rate of weaner Madura bulls. Twenty-four weaner Madura bulls were sourced from the heard at Beef Cattle Research Station that allotted to one of four treatments (A: null, B: 10 mg/kg dietary DM, C: 30 mg/kg dietary DM and D: 50 mg/kg dietary DM) with six replicates, all bulls offered elephant grass and concentrate diet (containing 13% crude protein and >70% total digestible nutrient). Bulls were measured (liveweight, body condition score, and hip height) every two weeks, blood samples were collected at the start and end of the experiment for metabolite and hormonal analyses. The results showed that no supplement effects were detected on liveweight gain, body condition score, and hip height gain (P>0.05). The treatments also did not increase the concentration of insulin-like growth factor-1 and triiodothyronine. Thus, weaner Madura bulls in the current experiment had a rapid growth rate although there was no supplement effect detected probably because not enough amount of zinc-lysine was provided. There was an improvement in the IGF-1 and T3 hormone concentrations in circulation after the experimental period which indicated a rapid growth of the bulls. Keywords: concentrate, grass, Madura cattle, organic-zinc, supplement. Share Link | Plain Format | Corresponding Author (RISA ANTARI)

37	Animal Nutrition and Feed Technology	ABS-66
The Effect of Feeding Different Dietary Fiber Sources on Eating Behavior and Rumination Activity of Brahman Cross Breed Calves During Weaning Transition Nurmeiliasari, Amir Husaini Karim Amrullah, Elvira Yunita,Yanuar Rahmad Ramadhan University Of Bengkulu Abstract Rumen development is one important factor that plays a significant role in raising healthy and productive calves. Feeding dietary fiber is essential to promote rumen growth, fermentation, chewing activity rumination behavior in young calves. This research evaluated the effect of feeding different dietary fiber sources on eating behavior and rumination activity of Brahman cross breed calves during weaning transition. Eight Brahman cross breed calves aged 8 weeks were randomly distributed into two different dietary fiber treatments. (T1 = concentrate 40 percents + Pennisetum purpureum cv. Taiwan 60 percents- P2 = concentrate 40 percents + Pennisetum purpureum cv. Taiwan 30 percents + Gliricidia sepium 30 percents). A similar formulation of concentrate was applied (crude protein 17 percents). A milk withdrawal was done gradually. Eating behavior and rumination were observed at from 8 am to 5 pm. After a month of dietary fiber sources feeding, observation on diurnal eating and rumination behavior showed changes on duration of eating time, percentage of eating time as well as eating frequency ( P<0.05). A Pennisetum purpureum cv. Taiwan fed group spent longertime eating than that of combination of Pennisetum purpureum cv. Taiwan 30 percents + Gliricidia sepium 30 percents treatment group (246,35 minutes vs 239,31 minutes). Of the 9 hours observation, 45,62 percents time was alocated for eating Pennisetum purpureum cv. Taiwan. A less percentage of time was spent for eating a combination of dietary fibers (44, 31 percents). A more frequent eating was observed in T1 group (20,88 times) than the T2 group (20,02 times). The treatments didi not affect the drinking time (P>0.05). The calves demonstrated similar amount of time in standing (427,20 minutes and 426,21 minutes, P >0.05). Feeding different fiber sources did not alter the amount of time of diurnal laying (135,27 minutes vs 136,06 minutes). A provision of higher fiber diet in T1 group increased rumination frequency (P<0.05). However, it did not affect duration and percentage of time of rumination during s diurnal observation (P>0.05). In conclusion, feeding Pennisetum purpureum cv Taiwan as a dietary fiber during weaning transition improve eating behavior and rumination activity of calves during weaning transition. Keywords: legume, young calves, eating behavior, rumination Share Link | Plain Format | Corresponding Author (Nurmeiliasari Nurmeiliasari)

38	Animal Nutrition and Feed Technology	ABS-68
FEED DEGRADABILITY OF LOCAL GRASSES IN THE RUMEN OF GOAT Ismartoyo*, Rohmiyatul Islamiyati*, Syahriani*, and Asmuddin Natsir* *Faculty of Animal Husbandry, Hasanuddin University Abstract The main aim of this experiment in sacco was to examine the characteristic degradation of four local grasses in the rumen of goat. Those of four grasses studied were R1=Elephant grass, R2= Mini elephant grass, R3=Panicum maximum grass, and R4=Brachiaria decumbens grass. Randomised complete design (RCD) was employed to allocate four fistulated goat. The protocol for this study was approved by the Ethical Review Committee of the Hasanuddin University Makassar, before the commencement of the experiment. Goat was kept in an individual metabolism crate and fed with general purpose diets. A general purpose (GP) diet was mixed with equal amounts of those 4 grasses Elephant grass (R1), Mini elephant grass (R2), Panicum maximum grass (R3) and Brachiaria decumbens grass (R4). The GP diet was added with 20% of rice bran. Water and salt were provided ad libitum. Voluntary feed intake was then measured every day by weighing feed offered at 7am and feed residue at 7 am in the next day. In order to determine the DM degradability, 3 g samples of four grasses were weighed in duplicates into standard nylon bags. The bags were incubated in the rumen and withdrawn after 4, 8, 12, 24, 48, and 72h. The result of this experiment showed that the average of feed voluntary feed intakes was dry matter 460.5g/d, organic matter 984.9g/d, and crude protein 139.8g/d, respectively. The dry matter loss at 72h after incubation was R1=55.7%, R2=55.6%, R3=53.2%, and R4=46.1%, respectively. The crude protein loss after 72h of incubation was 71.9%, 62.7%, 72.1%, and 62.5%, respectively. The characteristics degradation of R1was a=9.9%, b=64.4%, and c=0.05- R2 was a=16.5%. b=45.3%. c=0.04- R3 was a=7.4%. b=68.2%. and c=0.04- and R4 was a=6.7%, b=56.2%, and c=0.06. Mini elephant grass had the longest of lag time (4.2h) compared to that of other grass studied (average 1.2h). It is concluded that all of the local grasses investigated were acceptable, palatable, and readily degradable by rumen microbes in the rumen of goat. Therefor those of 4 grasses are a good feed resources for ruminant.r Keywords: Degradability, intake, grass, rumen, goat Share Link | Plain Format | Corresponding Author (ismartoyo ismartoyo)

39	Animal Nutrition and Feed Technology	ABS-70
Fermented Feed from Palm Kernel Meal Improves the Performance of Bali Cattle in Central Sulawesi Fery Fahruddin Munier (1)*, Wardi (2) and Mohammad Takdir (3) (1) Indonesian Center for Agricultural Technology Assessment and Development, Bogor (2) Research Center for Animal Husbandry, National Research and Innovation Agency, Bogor, Indonesia (3) Central Sulawesi Assessment Institute for Agricultural Technology, Palu *Corresponding author. Email: ffmunier[at]yahoo.com Abstract The success of the beef cattle business of community farms is to provide quality feed and have adequate nutritional content. Efforts can be taken by making concentrate feed based on agricultural products as an effort to provide relatively cheap and quality local feed. The purpose of the study is to add value to palm kernel meal as the main ingredient constituent of animal feed in the development of beef cattle breeding in Parigi Moutong. This research was carried out at the beef cattle development center in the Parigi Moutong Regency of Central Sulawesi Province. The livestock that will be used are 20 female Balinese cattle with an age range of 1.5-2.0 years. The study used a complete random design with treatment K: control (0% palm kernel meal + 0 % cocoa dust), P1: 16.5% palm kernel meal + 38.5% cocoa dust, P2: 27.5% palm kernel meal + 27.5% cocoa dust, P3: 38.5% palm kernel meal + 16.5% cocoa dust. The parameters observed are the chemical composition of the feed (crude protein, crude fiber, crude fat and materials without nitrogen extract, body weight gain and body conditions score. The research data were analyzed by variance analysis (ANOVA), the difference test was carried out with Duncan^s Multiple Range (DMRT). The T2 treatment was higher (41.17 kg) than T3 (40.81 kg), control (29.03 kg) and T1 (27.0 kg). The application of fermented feed from palm kernel meal can increase the weight gain of female cows and the appearance of body conditions score. Keywords: Bali cattle, Fermented feed, Palm kernel meal Share Link | Plain Format | Corresponding Author (Wardi Wardi)

40	Animal Nutrition and Feed Technology	ABS-72
Response of goat fed with ammoniated rice straw and Gliricidia sepium and Urea palm sugar block (UPSB) supplementation Endang Pudjihastuti, J. R. Bujung, Charles Kaunang, A. Lomboan, F. N. Sompie, L. J. M. Rumokoy Faculty of Animal Husbandry, Sam Ratulangi University Abstract This study was aimed to measure the effect of ammoniated rice straw and Gliricidia sepium^s feeding and UPSB supplementation on livestock productivity. We used a completely randomized design (CRD) with four treatments and four replications. The data was analyzed with analysis of variance (ANOVA) test and Duncan^s multiple range test. In this study, the parameters observed were dry matter intake (DMI), daily weight gain (DWG) and livestock blood profile. Mean DMI in this study ranged from 400.30-685.43 g/head/day. ANOVA test showed that the feeding of ammoniated rice straw and Gliricidia sepium, and UPSB supplementation had significant effect (p<0.05) on DMI. Post-hoc analysis showed that the R3 treatment was significantly different (p<0.05) and higher among other treatments (R0, R1 and R2). R2 treatment was significantly different (p>0,05) from R1 and R0 treatments. In this study, the mean DWG ranged 42.20-70.18 g/head/day. ANOVA test showed that the treatments had significant effect (p<0.05) on DWG. R3 treatment was significantly different (p<0.05) and higher than the other treatments (R0, R1 and R2) in relation to DWG. Post hoc analysis showed that R2 treatment was significantly different (p>0.05) from the R1 and R0 treatments. Regarding the goat^s hemoglobin level, we found no significant differences among treatments (p>0.05). Hemoglobin level in this study ranged 8.7 to 12.00 g/dL. Leukocyte count also showed no significant different among treatments (p>0.05) with value ranged from 69.80-68.22/mm3. Erythrocyte count ranged from 8.0 x 106 to 14.8 x 106/mm3 and the results were not significant different among treatments (p>0.05). Blood glucose level ranged from 60.25 to 60.55 mg/dL and not significantly different among treatemnts (p>0.05). Blood urea levels ranged from 21.55 to 29.18 mg/dL and were not significantly different among treatments (p>0.05). Total albumin levels ranged from 2.48 to 3.11 g/dL, meanwhile globulins ranged from 2.80 to 3.51 g/dL and were not significantly different among treatments (p>0.05). It was concluded that the feeding of ammoniated rice straw (50%) + Gliricidia sepium (50%) + 300 g UPSB gave an optimal response in relation to DMI, DWG and normal-ranged blood profile. Keywords: rice straw, ammoniation, Gliricidia sepium, UPSB, goat Share Link | Plain Format | Corresponding Author (drh. Endang Pudjihastuti)

41	Animal Nutrition and Feed Technology	ABS-73
EFFECT OF PARTICLE SIZE ON LOCAL FEED INGREDIENT FLOWABILITY Ichlasul Amal, Anis Salam, Abdul Alim Yamin, Jasmal Ahmari Syamsu Laboratory of Feed Industry and Technology, Faculty of Animal Science, Universitas Hasanuddin, Makassar, 90245 Indonesia Abstract This study aims to determine the flowability of local feed ingredients with different particle sizes. This study was arranged using a completely randomized design (CRD) factorial pattern 3 x 5 with three replications. The first factor (A) is particle size. The particle size is fine (mesh 26), medium (mesh 12), and coarse (mesh <12). The second factor (B) is local feed ingredients (rice bran, coffee hull, fish meal, coconut meal, and corn cobs). Each feed ingredient was ground using a milling machine with the type RD-85 DI-2S with a 200 kg/hour capacity and a sieve size of 5 mm. Based on variance, it was shown that the treatment of particle size and the interaction between particle size and various local feed ingredients significantly (P<0.01) affect the flowability of feed ingredients. The flowability of coarse ingredients was significantly different compared to medium and fine particle sizes. Fine and medium particle sizes were not significantly different. Thus, the particle size of feed ingredients greatly determines the flowability of various feed ingredients. Keywords: Flowability, local feed ingredient, particle size Share Link | Plain Format | Corresponding Author (Ichlasul Amal)

42	Animal Nutrition and Feed Technology	ABS-81
Effect of Calcium Supplementation in Feed Containing Shrimp Head Meal and Anchovies Head Meal on Egg Laying Performance and Egg Quality of Mojosari Ducks Jon Frison, Salfina Nurdin Ahmad, Gresy Eva Tresia, Eni Siti Rohaeni dan Bachtar Bakrie Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of Indonesia Abstract Calcium is an essential mineral for eggshell formation and muscle contraction, and a deficiency of it can lead to poor egg quality and lower egg production in laying ducks. This study aims to determine the effect of giving the mineral calcium in a ration containing shrimp head flour and Bilis fish head on the laying performance and quality of hatching eggs of Mojosari ducks. A total of 105 female Mojosari ducks and 15 male Mojosari ducks, aged 78 weeks, were randomly divided into 15 flocks (each flock consisted of 7 females and 1 male duck). There were three kinds of treatment, namely P1 (ratio of prawn head meal and anchovies meal 2:1 + 1% mineral), P2 (comparison of prawn head meal and anchovies meal 1:2 + 1% mineral)., P3 (control, without mineral). The results showed that giving calcium minerals to feed containing shrimp head meal and Bilis fish head meal had no effect (P>0.05) on the performance of laying hens such as feed consumption, egg production, egg weight. Likewise, calcium mineral supplementation did not make a significant difference to fertility and hatchability, but the reduction in egg weight was significantly different (P<0.05). Numerically, P2 tends to have a large egg production (53.77%) compared to P1 (51.07%) and control (51.07%). Based on this research, it can be concluded that the provision of feed based on shrimp head meal and anchovies meal can be done without the addition of calcium minerals. Keywords: fertility, hatchability, laying duck, mineral, weight loss Share Link | Plain Format | Corresponding Author (Gresy Eva Tresia)

43	Animal Nutrition and Feed Technology	ABS-82
Quality of Complete Ration for Beef Cattle Fermented with Garlic Addition (Allium sativum) A.Y. Sudarmanto(1), A. Rifai(1), M. Syahrul(1), Tilawati(1), M.I. Rusli(2), S. Syahrir(1,3) 1)Feed Chemistry Laboratory, Faculty of Animal Husbandry UNHAS 2)Student, Faculty of Animal Husbandry UNHAS 3)Correspondence Authors: Email: nanisyahrirn9[at]gmail.com Abstract Garlic contains active compounds that can inhibit the growth of pathogenic microorganisms. The addition of garlic is thought to maintain and even improve the quality of fermented beef cattle complete rations. This study aims to determine changes in temperature, physical quality and the level of damage to complete rations fermented and added garlic. Treatments consisted of P0: Complete ration without fermentation- P1 : Fermented complete ration- P2 : Fermented complete ration with addition of 1% garlic. The study used a completely randomized design with 3 treatments and 5 replications. The results of the analysis of variance showed temperature, color, texture, aroma and fungal contamination of fermented complete ration had no significant effect (P>0.05) on the complete ration added with 1% garlic. The results of the T test on the level of complete ration fermentation damage showed that there was no significant difference between P1 and P2. The conclusion of this study is the addition of 1% garlic to increase temperature and physical qualities such as color, aroma, texture, level of damage and fungal contamination in fermentation of complete rations is not different from fermented rations without the addition of garlic. Keywords: Garlic, Fermentation and Complete Ration Share Link | Plain Format | Corresponding Author (Aurelya Yulyanti Sudarmanto)

44	Animal Nutrition and Feed Technology	ABS-92
Performance and Activity of Alkaline Phosphatase of Local Chickens Receiving Zinc Oxide and Phytase Hardi Julendra (a*) , Hendra Herdian (a), Muhammad Ainsyar Harahap (a), Ade Erma Suryani (b), Lusty Istiqomah (b), Mohammad Faiz Karimy (b), Taufik Kurniawan (b), I Nyoman Guna Darma (c) and Arum Surya Pratiwi (4) (a) Research Center for Animal Husbandry. National Research and Innovation Agency, Cibinong, West Java, Indonesia. *julendra1969[at]gmail.com (b) Research Center for Food Technology and Processing. National Research and Innovation Agency, Yogyakarta, Indonesia (c) Study Program of Nutrition and Feed Sciences, Faculty of Animal Sciences, IPB University, Bogor, Indonesia (d) Department of Biology Yogyakarta University, Yogyakarta, Indonesia Abstract A mixture of zinc oxide (ZnO) and phytase was believed to improve zinc digestion in enhancing local chicken performance. This study has conducted the effect of the dietary addition of local chicken (IPB D1) fed with ZnO and phytase and its effects on chicken performance and alkaline phosphatase (ALP) activity. A total of 120 birds 7 wk old (male and female) were allocated to one of four dietary treatments i.e. (1) basal diet- (2) basal diet with 80 ppm ZnO/kg diet- (3) basal diet with 80 ppm ZnO/kg diet, and 500 FTU phytase/kg diet- (4) basal diet with commercial ZnO, and commercial phytase. Dietary treatments were represented equally across 24 cages of 5 birds per cage with a 1.5 m2 animal space allowance. Grouped chickens by sex and three classes of body weight uniformity, i.e., 775 - 830 grams (L) group, 1020 - 1182 grams (M) group, and 1200 - 1400 grams (H) group. The split plot design was performed with the mixture of zinc oxide (ZnO) and phytase level set as the main plot and chicken sex type as a subplot was used to control the variations with four treatments and 6 replicates. The result showed that there was a significant difference (p<0.05) between males and females performance and ALP activity. The addition of IPB D1 diets with 80 ppm of ZnO/kg diet and 500 FTU phytase/kg diet resulted in higher (p<0.05) body weight and feed conversion ratio (FCR) than other treatments. However, the feed consumption and ALP activity were not significant. In conclusion, receiving added zinc oxide and phytase in diets was the right choice to increase the performance and ALP activity of Local Chicken IPB D1. Keywords: alkaline phosphatase, chicken performance, phytase, zinc oxide Share Link | Plain Format | Corresponding Author (Hardi Julendra Julendra)

45	Animal Nutrition and Feed Technology	ABS-102
Forages Production and Carrying Capacity of Grazing Field in the Captive Breeding Area for Timor Deer (Cervus timorensis de Blainville 1882) at Wosu Village, Central Sulawesi M.S. Arifuddin, Damry, Najamudin, a)M. Mangun, Sirajuddin, Mirajuddin Faculty of Animal Science and Fisheries, Tadulako University, Palu 94119, Indonesia a)Corresponding author: mardiahmangun18[at]gmail.com Abstract A research has been conducted to determine the botanical types and composition and the carrying capacity of the grazing field of a captive breeding area for Timor deer (Cervus timorensis) at Wosu Village, Bungku Barat District, Morowali District, Central Sulawesi Province from April 26 to May 16, 2018. Forage samples were taken with a destructive sampling method using a pair of 1 x 1m frame. Dry matter content of the forage samples was analyzed at the Animal Nutrition and Feeding Laboratory, Tadulako University. Data were analyzed and results were presented descriptively. The results showed that the botanical composition of the was 83% grasses and 17% weeds. The grasses were dominated by Imperata cylindrica, Cyperus rotundus, Cyperus kyllingia Endl., and Paspalum conjugatum. The carrying capacity of the grazing field was about 4.14 deer/ha/year. In conclusion, the carrying capacity of the grazing field that can be kept in the breeding area (20ha) is 83 animals / year. Keywords: Timor deer (Cervus timorensis), feed, captive breeding, botanical composition, carrying capacity Share Link | Plain Format | Corresponding Author (Muhammad Sadik Arifuddin)

46	Animal Nutrition and Feed Technology	ABS-103
Chemical Composition and Production of Forages edible to Timor Deer (Cervus timorensis de Blainville 1882) in the Palu Valley M.S. Arifuddin1, a), Damry1, R. Utomo2, H. Hartadi2 1Faculty of Animal Science and Fisheries, Tadulako University, Palu 94119, Indonesia 2Afaculty of Animal Science, Gadjah Mada University, Yogyakarta 55288, Yogyakarta a) Corresponding author: sadik_arifuddin[at]yahoo.com Abstract A study was carried out from June to December 2011 to determine the chemical composition and production of forages edible to Timor deer (Cervus timorensis) in Palu, Indonesia. Grass forages were samples with a destructive sampling method using a pair of quadrants with an individual size of 1 x 1 m. For tree legumes, leaves samples were taken at 8 weeks, 12 weeks, and 18 weeks after an initial uniform cutting of the leaves. All samples were subjected to a standard proximate analysis for nutritional contents. The ^carrying capacity^ of the forages for Cervus timorensis was calculated with some assumptions. Results indicated that the ^carrying capacity^ of Pennisetum purpureum, native grass, Ipomea spp., and Zea mays for Cervus timorensis (head/ha/year) 59.96, 9.84, 23.02 and 69.16 , respectivley. For legumes, the highest capacity was for Leucaena leucocephala (3.7 head/ha/year) harvested at 6 weeks, while the lowest one is for Sesbania grandiflora (1.8 head/ha/year) harvested at 12 weeks. Keywords: Chemical composition, forages production, timor deer, Palu valley Share Link | Plain Format | Corresponding Author (Muhammad Sadik Arifuddin)

47	Animal Nutrition and Feed Technology	ABS-106
The Use of Garlic as Feed Additive in Local Concentrate Feed for Goats on In Vitro Digestibility of Dry Matter and Organic Matter Mita Arifa Hakim (a), Ummul Khasanah (b), Ahmad Rifai (c), Jamila Jamila (d), Syahriani Syahrir (e*), Asmuddin Natsir (f) a) Animal Feed Technology Study Program, Faculty of Vocational Studies, Universitas Hasanuddin, Makassar b) Animal Husbandry Study Program, Faculty of Animal Science, Universitas Hasanuddin, Makassar c) Department of Animal Nutrition, Faculty of Animal Science, Universitas Hasanuddin, Makassar *nanisyahrir[at]yahoo.co.id Abstract The study aimed to determine the effect of garlic (Allium sativum) as an herbal additive in goat concentrate feed made from local feedstuff on in vitro digestibility of dry matter and organic matter. The study was conducted using a completely randomized design with 5 treatments and 3 replications. Treatment T0 = control, T1 = concentrate feed + 0.025% garlic powder, T2 = concentrate feed + 0.05% garlic powder, T3 = concentrate feed + 0.075% garlic powder, T4 = concentrate feed + 0.1% garlic powder. The analysis of variance showed that the effect of giving garlic (Allium sativum) in goat concentrate feed made from local feedstuff had a significant effect (P<0.05) on dry matter and organic matter digestibility. The results showed that the addition of 0.1% garlic in goat concentrate made from local feedstuff increased in vitro digestibility of dry matter and organic matter. Keywords: Feed additive, Allium sativum, in vitro fermentation Share Link | Plain Format | Corresponding Author (Mita Arifa Hakim)

48	Animal Nutrition and Feed Technology	ABS-111
The Effect of Providing Mixed Feed Consisted of Dwarf Napier Grass and Indigofera zollingeriana Pruned at Different Ages on Feed Consumption of Kacang Goat Indrawirawan1,, Asmuddin Natsir1, Marhamah Nadir1, a), and Andi Ricky Wahyudi2 1Department of Animal Feed and Nutrition, Faculty of Animal Science, Hasanuddin University, Makassar 2Undergraduate Student of Faculty of Animal Science, Hasanuddin University, Makassar a) Corresponding author: marhamahnadir[at]gmail.com Abstract One factor that has determined the quality of forage as ruminant feed is the pruning age of the plant. Different pruning ages have affected the quality of forage, which has impacted the productivity of animals that have consumed the feed. This study aims to determine the effect of feeding a mixture of dwarf Napier grass and Indigofera zollingeriana pruning at different ages on the feed consumption of Kacang goats. Ten Kacang goats were randomly divided into two treatment groups, with five goats in each group. The first group (T1) was fed a mixture of dwarf Napier grass and Indigofera zollingeriana and was pruned at 40 days. The second group (T2) was fed a mixture of dwarf Napier grass and Indigofera zollingeriana and was pruned at 60 days. The observed variables included dry matter consumption, organic matter consumption, percentage of feed consumption per body weight, and ash consumption. The results showed that the treatment did not have a significant effect (P>0.05) on all Kacang goat feed consumption variables. The research concluded that feeding a combination of dwarf Napier grass and Indigofera zollingeriana, either pruned for 40 or 60 days, did not significantly affect feed consumption by Kacang goats. Two different pruning ages are applicable for combining Indigofera zollingeriana with other forages for Kacang goats. Keywords: dwarf Napier grass, feed consumption, Indigofera zollingeriana, Kacang goat, pruning Share Link | Plain Format | Corresponding Author (Indrawirawan Indrawirawan)

49	Animal Nutrition and Feed Technology	ABS-118
PleasForage Availability and Quality According to the Goat Breeders in North Minahasa Regency Ivonne Maria Untu- Wisje Lusia Toar- Laurentius JM Rumokoy Sam Ratulangi University Abstract The local production of goats played an important role tu support the animal meat availability in the market. On the other hand breeders continued to struggle to maintain their goat production. One of the obstacles faced by them was the availability of quality forage for the needs to their livestock, even though in this area there was quite abundant and varied forage for livestock. Therefore, a survey was conducted to detect the availability of forage and its quality according to breeders views. This research was a realized through a survey approach where the data was processed and presented descriptively. Fifty respondents from goat farmers in this area were interviewed to obtain information regarding the availability of forage and its quality according to their personal views. The results of this survey showed that: 22% of respondents strongly agreed with the statement that the older the forage was, the higher the crude fiber contented, while 24% agreed, but most (40%) undecided and 6% disagreed. Knowledge about forage quality was detected regarding the statement that Caliandra was a potetial source of protein for goats: 46% strongly-agreed, 36% undecided and 12% agreed, but there were still 6% who disagreed. 52% responded that forage goat food is totally available around them and 48% said it was quite available. If given examples of forage plants such as Caliandra sp, then 28% said they were totally available, while 54% said they were quite available, 12% said they did not know and 6% said there was little available, but 0% said none. If you ask about Pennisetum purpureum cv. Mott then 58% answered totally-available and 24% available enough and there were 8% who were not sure while 10% said not much. We concluded that to develop goat livestock production in this area, it is very necessary to be accompanied by assistance and technical counseling regarding the availability and quality of forage for the development of goat livestock. Keywords: local breed, forrage, feed, breeders Share Link | Plain Format | Corresponding Author (Ivonne Maria Untu)

50	Animal Nutrition and Feed Technology	ABS-130
The Effect of Kepok Banana Juice (Musa paradisiaca L.) Through Drinking Water on the Quality of Laying Chicken Eggs Florencia Sompie, 1),Jein R Leke 2*), Jacqueline Laihad3), Erwin Wantasen4), Linda Tangkau5) ,and Hapry Lapian6) Departemen of Animal Nutrition and Feed Science, Faculty of animal science, University Sam Ratulangi, Jl. Kampus Unsrat Bahu, Kota Manado, Sulut 95115, Indonesia. Departemen of Animal Production, Faculty of animal science, University Sam Ratulangi, Jl. Kampus Unsrat Bahu, Kota Manado, Sulut 95115, Indonesia. Departemen of Social Economic, Faculty of animal science, University Sam Ratulangi, Jl. Kampus Unsrat Bahu, Kota Manado, Sulut 95115, Indonesia Abstract This study aims to determine the effect of Kepok Banana Juice (Musa paradisiaca L.) through drinking water on the quality of laying chicken eggs. The experimental design used was Completely Randomized Design (CRD) with five treatments and five replications and 4 laying hens aged 58 weeks for each experimental unit. The treatments given in drinking water: R0 (without Kepok Banana Juice/KBJ), R1 20 ml/L KBJ, R1 40 ml/L KBJ), R2 60 ml/L KBJ, R3 80 ml/L KBJ. A total of 50 eggs were applied for quality analysis for 8 weeks. The variables studied were egg weight, egg yolk weight, egg white index, egg color, egg shell weight, egg shell thickness, and egg cholesterol. The results showed that Kepok Banana Juice up to 80 ml/l KBJ could increase egg weight, egg yolk weight, egg white index, egg color, egg shell weight, egg shell thickness, and reduce egg cholesterol. It can be concluded that 80 ml/1 liter of Kepk Banana Juice increased shell weight eggs, egg white weight and chicken egg white index MB 402. Keywords: Kepok Banana Juice, Egg Quality, Layer Chicken Share Link | Plain Format | Corresponding Author (Jein Rinny Leke)

51	Animal Nutrition and Feed Technology	ABS-147
THE EFFECT OF SUPPLEMENTED CLOVE LEAF MEAL ON BROILER PRODUCTITVITY Khaerani Kiramang, Asmuddin Natsir, Sri Purwanty, and Andi Mujnisa University of Hasanuddin Abstract Clove, a plant native to Indonesia, possesses the potential to act as an antibiotic. The presence of essential oils and phytochemicals in clove makes it a promising natural alternative for antibiotics in livestock, especially in poultry feed. The current study aims to investigate the impact of clove leaf powder supplementation as a replacement for growth hormone antibiotics on the productivity of broilers. The study^s outcome will provide valuable insights into the potential use of clove powder in poultry feed as an antibiotic substitute. The broilers were segregated into five treatment groups, and the clove leaf powder was administered for 30 days. The performance parameters, blood profiles, and internal organ profiles were used to measure productivity. The study employed an experimental approach using the General Linear Model Design with five treatments, repeated three times each. The data was analyzed through Manova, and the Wilks test was used for multiple comparisons to determine the differences in each treatment^s productivity. If there were any variations between treatments, the Tukey test was employed to identify the specific treatment that differed. The findings revealed that there were no significant differences in broiler response to the treatment between the measured parameters. Nonetheless, the broiler response remained within the standard range in all treatments. In light of the results, further research is necessary to explore the mechanism of action of clove leaf powder on broilers at various levels. Keywords: Cloves, Broiler, rations, antibiotics, productivity. Keywords: Keywords: Cloves, Broiler, rations, antibiotics, productivity. Share Link | Plain Format | Corresponding Author (Khaerani Kiramang)

52	Animal Nutrition and Feed Technology	ABS-161
CARCASS WEIGHT, TOTAL INTESTINE BACTERIA AND DIGESTIBILITY OF BROILER FED BIOPEPTIDES FROM CHICKEN CLAW Sri Rahayu*, Bambang Hartoyo, Titin Widiyastuti, FM. Suhartati, Muhamad Bata, Efka Aris Rimbawanto1 Faculty of Animal Sci., University of Jenderal Soedirman (UNSOED)-Indonesia Abstract The objectives of this research was to evaluate the effect of biopeptide supplementation from chicken claw on carcass weight, total intestinal bacteria and digestibility of dry matter (DDM) and organic matter (DOM) of broilers. Experimental research using completely randomized design and five times repetition. The research material were 200 DOC strain CP-707 that were reared up to 35 days of age and the bio-peptides was obtained through hydrolysis of chicken claw collagen using a commercial papain enzyme. Feed treatments were supplemented by bio-peptides in the amount of 0, 2, 4, and 6%. The polynomial orthogonal was applied if the treatments have a significantly effect on the measured variables. Supplementation of bio-peptides from chicken feet had a very significantly effect (P<0.01) on DOM and carcass weight and had significantly effect (P<0.05) on DDM and total bacterias. Bio-peptides supplementation up to the level of 6% in broiler ration improved carcass weight, DDM, DOM and total intestinal bacteria of broiler chickens. Keywords: biopeptides, chicken-claw, digestibility, bacteria, carcass Share Link | Plain Format | Corresponding Author (SRI RAHAYU RAHAYU)

53	Animal Nutrition and Feed Technology	ABS-166
Digestibility of Crude Protein and Crude Fiber of Cow^s Fur Flour in The Grower Phase of Quail (Coturnix coturnix japonica) Nancy Lahay(1)* Anie Asriany (1), Khuriatul Jannah Basir (2) 1) Department of Animal Nutrition and Feed, Faculty of Animal Husbandry, Hasanuddin University 2) Undergraduate Student, Faculty of Animal Husbandry, Hasanuddin University Abstract Cow^s fur flour is one of the slaughterhouse wastes that can still be used as a protein source feed ingredient because it has a protein content of 76.77%. The purpose of this study was to determine the digestibility of crude fiber and crude protein of quails given rations with the addition of cow^s fur flour. This study used 80 quails placed in cages with five cages per cage plot, feeding and drinking water were carried out by adlibitum. The research design used was a Complete Randomized Design (CRD) with 4 treatments and 4 replications, PO: Ration without cow^s fur flour, P1: Ration containing 2% cow^s fur flour, P2: Ration containing 4% cow^s fur flour. P3: The ration contains 6% cow^s fur flour. The observed parameters are the digestibility of crude protein and the digestibility of crude fiber. The results showed that the addition of cow^s flour in the ration had no real effect (P>0.05) on the digestibility of crude protein and had a real effect (P<0.05) on the digestibility of crude fiber. Based on the results of research that has been carried out, it can be concluded that the use of 6% cow^s fur flour in quail rations provides the highest protein digestibility value and the lowest crude fiber digestibility obtained at 2% feather flour use treatment. Keywords: Quail, Crude Protein Digestibility, Crude Fiber Digestibility, Cow Feather Flour Share Link | Plain Format | Corresponding Author (Nancy Lahay)

54	Animal Nutrition and Feed Technology	ABS-168
CHARACTERISTICS TESTS ON UREA MOLASSES BLOCK ADDITIONED WITH DIFFERENT ADHESIVES Jamila Mustabi1), Gustina Rahayu2) and Asmuddin Natsir1) Hasanuddin University Abstract Abstract Urea Molasses Block (UMB) is a supplementary food composed of several nutritious feed ingredients needed by livestock. However, the texture is easily damaged, broken, so that the adhesive is needed. Adhesives commonly used are cement but are not safe to use so natural adhesive materials such as sago, cassava, tapioca can be used because they have a relatively high starch content. The purpose of the study was to evaluate the physical characteristics of UMB by using different adhesives. This study uses a completely randomized design factorial pattern consisting of three replications with two factors. The first factor is the type of adhesive P1 = cement- P2 = cassava- P3 = tapioca and P4 = sago. The second factor of storage time is W1 = 0 days- W2 = 7 days and W3 = 30 days. The results of this study indicate that the addition of different adhesives had no significant effect (P> 0.05) on water content and UMB density but had a significant effect (P <0.05) on the storage duration. The addition of different adhesives had a significant effect (P <0.05) on water absorption and UMB storage time. The conclusion of this study is that UMB with the use of sago flour adhesives has the best moisture content, density and water absorption and can last for 30 days Keywords: Cassava, Cement, Tapioca, Sago, Urea Molasses Block Share Link | Plain Format | Corresponding Author (Jamila Mustabi)

55	Animal Product Technology	ABS-8
Sensory characteristics of grilled beef marinated using seasonings containing Etlingera elatior flower with different length of marination Tuti Suryati, Alfin Ahadin, Muhammad Ibaadurahman, and Muhammad Arifin Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia Abstract The objectives of this study was to evaluate sensory characteristics of grilled beef marinated using seasonings with or without Etlingera elatior flower (EEF) addition with different length of marination. This study used beef shortplate from the same breed and age. The spices used include garlic, onion, ginger, chili, pepper, honey, vinegar, salt and sesame oil. EEF are added as much as 10% of the total spices for seasoning treatment with the addition of EEF. The meat was sliced with a thickness of 3.5 mm, and then treated without marination (control), marination with spices without EEF addition (standard seasoning), or marination with standard spices plus EEF. The marination was done for 30 mins, 60 mins, 12 hrs or 24 hrs. The study was designed using a completely randomized design with different combinations of marinade treatments and marination times. The panelists involved were 35 semi-trained panelists. The sensory tests carried out included hedonic (preference) tests and hedonic quality tests which were then analyzed nonparametrically using the Kruskal-Wallis and Dunn test. Based on the hedonic test for all attributes (color, aroma, tenderness, general appearance and taste) the result showed that marinating for 30 mins or 24 hrs using seasoning without EEF produced more preferred grilled meat (P<0.05) than other treatments. The grilled beef with seasoning added with EEF for 12 or 24 hrs are more preferred (P<0.05) than others marinade using seasoning with EEF. Marinating treatment without EEF for 30 minutes, 60 mins and 24 hrs resulted in the higher tender (P<0.05) than others, namely tender to very tender (score 4 to 5). As a conclusion, taking into account of time efficiency in terms of flavor, taste and tenderness the best marination duration for marination using seasoning without EEF is 30 mins, and for seasoning with EEF is 12 hrs. Keywords: Etlingera elatior, grilled beef, marinade, sensory characteristics Share Link | Plain Format | Corresponding Author (Tuti Suryati)

56	Animal Product Technology	ABS-13
Hatching Machine Temperature Pattern Against Hatching Ability,Weight and Time Sikumbang Janti Duck Eggs Tertia Delia Nova, Linda Suhartati, Firda Arlina 1Depatyment Technology and Animal Production, Animal Science Padang, Universitas Andalas , 163125 2Depatyment Technology and Animal Production, Animal Science Payakumbuh, Universitas Andalas 3Departmen Tehnology and, Animal Science, Universitas Andalas Abstract This study aims to determine differences in the pattern of setting the temperature of the incubator on egg shrinkage, hatching time, hatching weight, hatch-ability and mortality of Sikumbang Janti duck embryos. In this study using experimental methods with descriptive data analysis, calculating each percentage value of the 3 treatments. The pattern of the hatching machine temperature adjustment is P1 (37.50C (days 1-25) and 370C (days 26-28)), P2 the temperature is increased by 400C (days 22-24) for 3 hours per day, P3 the temperature is increased by 420C ( days 22-24) for 3 hours per day. The variables observed were egg shrinkage, hatching time, hatching weight, hatch ability and embryo mortality. The results of this study showed that the percentage of egg shrinkage was between 12.37-13.21%, hatching time was between 630.35-680.37 hours, hatching weight was between 41.94-45.20 grams, hatch ability was between 28.33-71. ,67%, third-phase mortality 15.00-40.00% and late-phase mortality 15.68-52.77%. The conclusion of this study is that the 40oC hatching machine temperature regulation pattern (days 22-24) during hatching is more effective and efficient in achieving optimal hatching. Keywords: temperature, incubating machine, kumbang janti duck, weight, hatchability. Share Link | Plain Format | Corresponding Author (Tertia Delia Nova)

57	Animal Product Technology	ABS-56
Chemical Characteristics and Antioxidant Activities of Chicken Head Protein Hydrolysate Pramudya Andiana(1*), Ghofar Aji Prabowo(1), Khothibul Umam Al Awwaly(2), Aris Sri Widati(2), and Eny Sri Widyastuti(2) 1Student of Animal Product Technology Department, Faculty of Animal Science, Universitas Brawijaya, Malang-65145, Indonesia *ramudyaandiana[at]student.ub.ac.id 2Lecturer of Animal Product Technology Department, Faculty of Animal Science, Universitas Brawijaya, Malang-65145, Indonesia Abstract Abstract. Chicken head is rich in protein that has a potential source of antioxidant peptides. However, researches about antioxidant activities from chicken head protein hydrolysate are still limited. The purpose of this research is to analyze the chemical characteristics and antioxidant activities of chicken head protein hydrolysate produced by papain at different hydrolysis time. The method of this research is an experimental method with a Completely Randomized Design (CRD) with four treatments consisting of T0 (without hydrolysis), T1 (2 hours of hydrolysis time), T2 (4 hours of hydrolysis time), and T3 (6 hours of hydrolysis time). The data obtained is analyzed using Analysis of Variance (ANOVA). Then, if there is a significant difference in each treatment, it will be continued with the Duncan^s Multiple Range Test (DMRT). The difference of hydrolysis time on chicken head protein hydrolysate produced by papain shows a highly significant difference (P<0.01) on pH value, reducing power, and ferrous ion chelating activity, a significant difference (P<0.05) on peptide content and degree of hydrolysis (DH), and no significant difference (P>0.05) on IC50 of DPPH radical. The results in T3 also show the strongest antioxidant activities. Keywords: antioxidant, head chicken, protein hydrolysate Share Link | Plain Format | Corresponding Author (Pramudya Andiana)

58	Animal Product Technology	ABS-69
Purification and Characterization of the Lon-Like Recombinant Protease of Lactobacillus Plantarum IIA-1A5 Olfa Mega a*), Cece Sumantri b), Irma Isnafia Arief b), Cahyo Budiman b) a) Faculty of Animal Science, University of Jambi * olfa_mega[at]unja.ac.id 2) Department of Animal Production and Technology, Faculty of Animal Science, IPB University Abstract Lon protease is an enzyme responsible for degrading proteins. Lon-like proteases have the same effec as Lon. The gene encoding the Lon-like IIA-A5 was obtained from the genome of the bacterium, Lactobacillus plantarum IIA-1A5. The Lon-like IIA1A5 gene was cloned into the pET-28a (+) vector and expressed in Escherichia coli BL21(DE3) competent cells. The L. plantarum IIA-1A5 is a native Indonesian strain and has proteolytic activity in meat, processed meat products and casein. The study aims to purify and verify the proteolytic activity of Lon-like proteases produced by L plantarum IIA-1A5 in degrading animal product proteins. We used HisTrap SP FF 5 mL column with different imidazole concentrations for purification, namely 10- 50- 100- 150- 200- 250- 300- 350 and 500 mM. Then, the purified Lon-like IIA-1A5 was tested for optimal pH and temperature, and its ability to the hydrolyzed animal product proteins. The results showed that the specific activity, purity level, and yield of the purified Lon-like IIA-1A5 were 828.96 AU/mg, 2.2 times, and 224% respectively. The molecular weight of this protein was confirmed by SDS-PAGE of 43.76 kDa. The proteolytic activity of the enzyme is optimal at pH 9 and a temperature of 60 oC. Lon-like IIA-1A5 can generally degrade protein animal products. However, the enzyme can hydrolyze gelatin, myofibrillar protein, collagen, and sarcoplasmic protein better than casein and skim milk. Keywords: Purification, Characterization, Lactobacillus Plantarum, Lon-like Protease Share Link | Plain Format | Corresponding Author (Olfa Mega)

59	Animal Product Technology	ABS-74
THE INFLUENCE OF THE RATIO OF POTATO TO WHEAT FLOUR ON THE PHYSICAL PROPERTIES OF DANGKE CRACHERS Herwandi- Ratmawati Malaka*- Fatma Maruddin- Naharia 1Undergraduate Student of Animal Husbandry, Hasanuddin University 2Departement of Animal Production, Faculty of Animal Science, Hasanuddin University Abstract Dangke is one typical food of South Sulawesi that made from Buffalo^s milk or Cow^s milk. The purpose of this study was to determine effect of ratio of wheat flour and potatoes on organoleptic characteristics (crispness, aroma, taste and preference), and the protein content of dangke crackers. The study used a completely randomized design (CRD) in one direction with 5 replications with 3 treatments with the addition of 0%, 15%, 30% potatoes. The results of the study indicated that the aroma, taste and protein increased at 30%, while the crispness and favoritability decreased as more potato substitutes were added to 30% of dangke crackers. The addition of potatoes by 30% can increase the aroma, taste and protein in dangke crackers. Keywords: Milk, Dangke crakers, potatoes, organoleptic characteristics Share Link | Plain Format | Corresponding Author (Ratmawati Malaka)

60	Animal Product Technology	ABS-75
WPC, Hydrocoloid, and Their Combination Improve The Quality of Low-Fat Cheese Triana setyawardani- juni sumarmono, hidayah dwiyanti Animal science faculty, Jendral Soedirman University Dr, Suparno street No. 60 Grendeng Purwokerto , Jaw Tengah Indonesia 53122 Abstract The quality of low-fat cheese can be improved by adding fat replacers made from carbohydrates (hydrocolloids) and protein. In the study, protein, specifically WPC, and different kinds of hydrocolloids were given the following treatments: As a control, the study used cheese made from full milk (full fat) made from fresh milk. The other 5 treatments were: P1: Cheese from low-fat milk + fat substitute (0.5%) WPC- P2: Cheese from low-fat milk + fat substitute (0.5%) carrageenan- P3: Cheese from low-fat milk + fat substitute (0.5%) porang flour- P4: Cheese from low-fat milk + fat substitute (0.5%) WPC + 0.5% carrageenan- and P Each therapy was done 3 times. The things that were looked at were the cheese^s color, total solids, whey %, and titrated acid. The results showed that Hue and Chroma didn^t alter between the control and treatment colors, but the Whitness Index did. (WI). The percentage of whey and total titrated acid was changed between treatments, but not in the control, and the total solids were mostly the same. The study^s conclusion is that adding wpc, hydrocolloid, alone or together, has the smallest whey index features at P1, the least TAT at P0, and the smallest percentage of whey at P2 Keywords: Please Just Try to Submit This Sample Abstractlow-fat cheese- replacer, carbohidrates, protein Share Link | Plain Format | Corresponding Author (Triana setyawardani setyawardani)

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