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91	Animal Reproduction	ABS-153
Semen Quality of Bali Cattle diluted by four different diluents A.L. Toleng1, Yusuf, M1, Sahiruddin, S1, Masturi1, Hasrin2, Musdalifah3, Arsan,J4 and Milawati1 1.Laboratory of Animal Reproduction, Faculty of Animal Science, Hasanuddin University, Makassar. 2.Department of animal production, Faculty of vocation, Hasanuddin University 3.Department of Animal Production, Faculty of Agriculture, Muhammadyah University, Rappang 4 Department of Animal Production, Faculty of Science and Technology, Islam State University, Makassar. Abstract : Semen diluents are well known as an important factor in maintaining semen quality. This study was conducted to measure the semen quality of Bali bulls diluted by using four different diluents: andromed (commercial diluent), tris aminomethane egg yolk (Tris EY), tris aminomethane egg soybean milk (Tris SM) and Tris EY + Tris SM. Semen from one Bali bull were collected weekly for 4 weeks by artificial vagina. The fresh collected semen were analyzed for macroscopic and microscopic analysis. The semen were divided into 4 groups and diluted with the 4 diluents, respectively. The diluted semen were divided into 2 groups, liquid and frozen semen. Observation of liquid semen quality was carried out up to 144 hrs in a cold storage. The frozen semen were evaluated 1 week after freezing in liquid nitrogen. Motility, viability, abnormality, intact membrane (IM) and intact acrosome (IA) of the sperm were analyzed by Computerized Assisted Semen Analysis (CASA). Mean different between groups for all parameters were analyzed by statistical analysis. Fresh semen motility, viability, Abnormality, IM and IA were 5.43+0.83 ml, 87.62+2.79 %, 1.33+0.38x109, 10.49+2.9 %, 85.65+0.67% and 79.98+5.69%, respectively. In the diluted fresh semen, sperm viability for the andromed diluted semen (87.0+2.1%) was significantly (P<0.05) higher in compared to other three diluents. However, other parameters (Motility, abnormality, IM and IA) were not statistically different between the 4 semen diluents. In the frozen semen, semen motility tended (P<0.1) to be higher in andromed diluted semen (59.8+6.7%) in compared to those in other semen diluents. Viability and intact membrane were significantly (P<0.05) higher in andromed diluted semen in compared to those other 2 diluents. Semen diluted with the 4 semen diluents and storage in the cold storage could maintain motility, viability and IM more than 40% for 144 hrs and maintain IA for 96 hrs. Sperm abnormality less than 25 % could be maintained for 84 hrs for andromed diluted semen in the cold storage in compared to only 48 hrs for semen diluted with the other 3 diluents. It can be concluded that all the 4 semen diluents could be used for Bali semen dilution, however, Andromed was more better in increasing sperm motility for fresh liquid semen and sperm motility, viability and intact membrane for frozen semen. Moreover, the andromed diluted semen could maintain the low sperm abnormality longer during the cold storage. Keywords: Bali bulls, semen diluents, semen motility, viability and abnormality Share Link | Plain Format | Corresponding Author (Abd. latief Toleng)

92	Animal Reproduction	ABS-156
The Potential of Bali Cattle Oocytes from Ovaries of Slaughterhouses in Makassar, South Sulawesi Herry Sonjaya1,a), Hasbi Hasbi1), Sudirman Baco1), Hikmayani Iskandar2), and Erni Damayanti3) 1Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia 90245 2Research Center for Applied Zoology (BRIN), Cibinong, West Java, Indonesia 16911 3Laboratory Embryo In vitro faculty of Animal Science, Hasanuddin University, Makassar, Indonesia 90245 Abstract Bali cattle are native Indonesian cattle that are kept by many breeders in South Sulawesi. Utilization of Bali cattle ovaries from slaughterhouses is one way to maintain the genetic quality of Bali cattle. This study aims to examine the potential of the ovaries of Bali cattle from a slaughterhouse in Makassar, South Sulawesi. The research method was that the ovaries were taken from the abattoir below to the laboratory using 0.9 persen NaCL and gentamicin. Then they were collected using the slicing technique. Oocytes were collected under a microscope and graded based on the compactness of cumulus cells. The results of this study were grade A 33.67 9.81, grade B 20.67 8.08, grade C 20.67 8.08, and grade D 16.67 9.07. The number of oocytes with grades A and B was higher than grades C and D. Therefore, it can be concluded that oocytes obtained from abattoirs in Makassar have the potential for in vitro embryo production. Keywords: Bali cattle- ovaries- slaughterhouses- graded Share Link | Plain Format | Corresponding Author (Herry Sonjaya)

93	Animal Reproduction	ABS-160
MOTILITY, VIABILITY AND FERTILITY OF KAMPUNG ROOSTER SPERMATOZOA IN SKIM MILK BASED SEMEN EXTENDER Dadang Mulyadi Saleh, Mas Yedi Sumaryadi, Aras Prasetiyo Nugroho, Chomsiatun Nurul Hidayah and Agus Yuniawan Isyanto Faculty of Animal Science, University of Jenderal Soedirman Abstract Keywords: kampung rooster spermatozoa, motility, viability, fertility, skim-milk Share Link | Plain Format | Corresponding Author (Dadang Mulyadi Saleh)

94	Animal Waste and Environmental Technology	ABS-1
Effect of Additional Sources of Carbon and Nitrogen in Liquid Waste of Dairy Cows on The Quality of Organic Soil Conditioner Wahyu Suradi Pranata (a), Eulis Tanti Marlina (b), Yuli Astuti (b), Ellin Harlia (*b) a) Postgraduate Study Program, Faculty of Animal Husbandry, Padjadjaran University, Sumedang, Indonesia b) Faculty of Animal Husbandry, Padjadjaran University, Sumedang, Indonesia *ellin.harlia[at]unpad.ac.id Abstract Studies on the processing of dairy farm wastewater have been carried out using samples from liquid waste storage ponds. The purpose of this study is to process liquid waste by fermentation to become a liquid organic soil conditioner in accordance with Ministerial Decree of Agriculture No. 261 of 2019 concerning Minimum Technical Requirements for Organic Fertilizers, Bio Fertilizers and Soil Conditioner. Organic soil conditioner can be used as soil fertilizer, by way of land application. Liquid organic soil conditioner can be obtained by fermenting liquid waste coupled with C-organic including milk sludge and silage fluid starter. The results of fermentation for 21-28 days showed that the content in C-organic liquid waste was close to 10, pH, heavy metal content, micronutrients, and negative E. coli and Salmonella bacteria had met the requirements of the standard of Ministerial Decree of Agriculture No. 261 of 2019. Keywords: liquid waste, dairy cows, carbon sources, nitrogen sources, organic soil conditioner Share Link | Plain Format | Corresponding Author (Wahyu Suradi Pranata Pranata)

95	Animal Waste and Environmental Technology	ABS-22
Purification of Quality and Quantity Biogas with Different Types of Animal Manure by Condensation and Multilevel Adsorbtion Method Arina Manasikana1, b) and Mochammad Junus2, Osfar Sjofjan2, a), Sri Minarti2 Brawijaya University Abstract Biogas is renewable energy that can be seen from technical, economic, and social aspects which can be used to supply energy that comes from fossil fuels. Biogas contains methane (CH4) and carbon dioxide (CO2) as well as several other gasses, namely hydrogen sulfide (H2S), ammonia (NH3), hydrogen (H2) and nitrogen (N). Biogas can be made from livestock waste such as animal manure by ruminants, pseudoruminants and non-ruminants. Therefore, this study aims to compare the quality and quantity biogas before and after purification from different types of animal manure by condensation and multilevel adsorption method. Types of animal manure used in the research by cow dung, rabbit manure, broiler manure, and mix manure. The study was conducted in 7 treatments, namely cow dung (T0), broiler manure (T1), rabbit manure (T2), cow dung+broiler manure (T3), cow dung+rabbit manure (T4), broiler manure+rabbit manure (T5), and cow dung+broiler manure+rabbit manure (T6). Then all treatments will be purified with condensation and multilevel adsorption methods with 5 different adsorbents (roof tile waste (RW), CaO (CO), activated carbon (AC), Fe powder (FP), and zeolite (Z)). The methods were the preparation of feedstock, purification tools, biogas production. The parameters measured were volume, pressure, and biogas content before and after purification. The research design used was a Completely Randomized Design (CRD). The results obtained were tasted by One Way ANOVA followed by DMRT Test with a confidence 95% (&#945-=0.050. Furthermore, the effect of different types of animal manure on quality and quantity biogas is the highest CH4 and lowest CO2 content resulting from purification using A3 treatment of 98.73% CH4 and 0.70% CO2 with T6 (mixture of 3 animal manure). However, the pressure, flow rate and volume of biogas with different types of waste have different values every week. Keywords: purification, biogas, animal manure, condensation, multilevel adsorption Share Link | Plain Format | Corresponding Author (arina manasikana)

96	Animal Waste and Environmental Technology	ABS-112
Characteristics of Compost Mixture Feces of Dairy Cattle and Laying Hens Using Bamboo Shoots as a Bioactivator Muhammad Irfan Said, Endah Murpi Ningrum, Nahariah, Dinda Gita Andrayana, Muhammad Tang Laboratory of By-Products Processing Technology, Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar 90245 Abstract The use of compost as a source of plant nutrients is growing rapidly. This is supported by the increasing need for food by humans. Agricultural land requires sufficient and sustainable nutrients so that food needs are maintained. Compost can be produced from the feces of ruminants and non-ruminants. It is hoped that the combination of the two types of stool will complement each other. The use of natural materials in the form of bamboo shoots has been used as a bioactivator in the compost fermentation process. This study aims to evaluate the properties of compost from the feces of dairy cattle (DC) and laying hens (LH) using bamboo shoots as a bioactivator for the fermentation process. A completely randomized design with a 3x2 factorial pattern was used as the basic design. The combination of feces of dairy cows (DC) and laying hens (LH) is the first factor. The treatment consisted of different combination ratios (DC:LH), TA(100%:0%)- TB (50%:50%) and TC(0%:100%). Bioactivator level (B) is the second factor, B1(1%) and B5(5%). Repetition is done 3 times. (1) pH values, (2) levels of C-organic, (3) N-total, and the (4) C/N ratio were used as research parameters. The results showed that the difference in the ratio of the combination of feces and the level of bioactivator led to an increase in C-organic and N-total content and a decrease in the pH value and C/N ratio of compost. Treatment of TB(50%:50%) with decomposer level B5(5%) produced compost with the best properties and closer to soil properties. Keywords: Compost- Dairy cattle feces, Laying hens feces- Bioactivator- Bamboo shoots Share Link | Plain Format | Corresponding Author (Muhammad Irfan Said)

97	Animal Waste and Environmental Technology	ABS-120
Optimizing the composting of market vegetable waste as a source of organic fertilizer using various types of microorganisms Anie Asriany Department Nutrition and Animal Feed, Faculty Animal Husbandry, Hasanuddin University Anieasriani1994[at]gmail.com Abstract Market vegetable waste reaches 75% of the total waste in the form of organic waste and 25% is an organic waste. Composting using local microorganism is expected to reduce waste volume. The purpose of this research is to analyze kind of local microorganism to acceleratethe decomposition of organic matter of vegetable waste, analyzing local microorganism concentration on accelerate of decomposition of organic matter of vegetable waste, analyzed the intraction between type of local microorganism and concentration of local microorganismon the acceleration of decomposition of organic mater. The experiments were arrangel in aFactorial Randomized Completely Block Design (RCBD), which consisted of three typesol local microorganism (yeast, banana head, end snail) witht three level concentration (400 Ml, 600 Ml, 500 Ml) treated divided into three blocks as replicates. The results showed that : The highest temperature (35,0C) was in the 12 thday, the composting process was not achieved thermophilic temperature. Local microorganism from banana culm at 400 Ml, dosage accelerated the weight loss of organic matter (16,65%) , the most refined texture. Banana local microorganism spinach at a dose 0f 600 Ml, showed the highest levels of calcium (2.18%) carbon contend (12. 35%), and reduced C/N ratio to lowest 6.05 compared with the other treatments. Banana local microorgamism at 600 Ml, doseshowed a darker compost color. Keywords: Local mikroorganisme, compost, organic wast Share Link | Plain Format | Corresponding Author (Anie Asriany)

98	Extension of Agriculture	ABS-99
NEED OF INFORMATION AND TECHNOLOGY OF GOAT FARMER TO DEVELOP OF BANK FECES IN POLMAN REGENCY Please Just Try to Submit This Sample AbstractSyahdar Baba, Hastang, Aslina Asnawi, Muhammad Ihsan Dagong, Hendra, Indrawirawan, Aulia Uswa Noor, Muhammad Risal, Sitti Sohrah, Hassani Faculty of Animal Science Hasanuddin University Animal Science Study program, Maros Moslem University Farmer Group Amanah Abstract Bank of Feces is an integrated and connected management of livestock waste between breeders where they act as customers and beneficiaries of the utilization of livestock waste. The purpose of this study was to find out the information needed by breeders in the development of bank of feces in Polman district. This research was conducted in February - March 2023 in Tanda Sura Village, Tinambung District, Polman district. This location was chosen because it is one of the centers of small-scale goat farming. Data was collected through in-depth interviews and focus group discussions (FGD). FGDs are carried out by involving the heads of livestock groups, breeders, innovators, extension workers accompanying breeders and the Head of Village. The focus of the research is the information and technology needs of breeders in the development of feces bank. Data from FGD and in-depth interviews were analyzed and grouped by category. The results showed that the information needed by farmers about bank of feces are the benefits of feces bank, the mechanism of rights and obligations of breeders, the rights and obligations of off takers, determining the price of feces and urine, determining the price of fertilizer, management mechanisms and financing mechanisms. Bank of Feces will develop if there is transparency between off taker, farmer group and breeders as actors. Keywords: Bank of Feces, Need assessment, Goat farmer, organic fertilizer Share Link | Plain Format | Corresponding Author (Syahdar Baba)

99	Extension of Agriculture	ABS-101
Behavior of Beef Cattle Breeders in Selling Productive Cows in Tanete Riaja District, Barru Regency, Barru Iin Mulyawati Daryatmo, Syahdar Baba, M Ikrar MS Khon kaen University Faculty of Animal Science Universitas Hasanuddin Abstract The purpose of this study was to determine the behavior consisting of attitudes, subjective norms, and farmer behavior control in selling productive cows. The type of research used in this research is descriptive quantitative research. The sample that used in this research are 93 from 1,420 total population of beef cattle^s breeders in the hope village, Lompo Riaja, and Libureng Village, Tanete Riaja Subdistrict were calculated by using Slovin formula. Data analysis used in this study is descriptive statistics using the Liker Scale. The results showed that: (1) attitudinal behavior was in the high category, which meant that the attitude of the farmer had known the negative impact that would occur on beef cattle business if selling productive cows, (2) subjective norm behavior was in a high category which meant that high social pressure obtained by farmers so that the sale behavior of productive female cattle occurs- (3) behavior control was in a high category which means that farmers have a great opportunity to behave in the sale of productive female cattle because of their perception of the ease of selling productive cows. Keywords: Attitudes, Subjective Norms, Behavior Control, Sales of Productive Female Share Link | Plain Format | Corresponding Author (Syahdar Baba)

100	Extension of Agriculture	ABS-150
USING OF CYBER EXTENSION MEDIA TO INCREASE FARMERS^ KNOWLEDGE IN UTILIZING RICE STRAW WASTE AS FEED FOR BEEF CATTLE Agustina Abdullah, Syahdar Baba, Sitti Nurani, Amidah Amrawati,Tanrigiling Rasyid, Kasmiyati Kasim dan Ilham Syarif Laboratory of Animal Husbandry Extension and Sociology, Faculty of Animal Husbandry, Hasanuddin University Abstract The research aims to determine the use of cyber extension media in increasing farmers^ knowledge of utilizing rice straw waste as beef cattle feed. The research was conducted in Barru District, South Sulawesi Province. Determination of farmer groups as respondents were carried out purposively on the middle livestock group. The research data was collected by conducting a survey using collection techniques, namely questionnaires, focus group discussions, and in-depth interviews (in-depth study) with several key informants. The results showed that 80% of respondent farmers needed to learn about cyber extension media to obtain information on using rice straw waste as beef cattle feed. In general, the respondent farmers only get sources of information related to using rice straw waste as beef cattle feed through extension workers whose intensity is limited because it has a large working area. Sometimes the material provided does not meet the needs of farmers, so the respondent^s farmer knowledge of the use of rice straw waste as animal feed for beef cattle still needs to be higher (45%). However, it has changed after being given information related to cyber extension counseling media to obtain information related to the use of rice straw waste as beef cattle feed. Knowledge of farmers who previously did not know about using rice straw waste as beef cattle feed finally became tofu. This is because farmers are enthusiastic about learning what they do not know about and want to continue studying and implementing the utilization of rice straw waste as beef cattle feed. For this reason, increasing and optimizing the utilization of rice straw waste as beef cattle through cyber extensions at the smallholder farm level is necessary. Keywords: cyber extension media, farmers, rice straw waste, Share Link | Plain Format | Corresponding Author (Agustina Abdullah)

101	Grassland Science	ABS-9
Productivity of three Benggala grass cultivars (Panicum maximum sp.) Which is cultivated as an intercropping in 15 years old palm oil plantations Iwan Herdiawan1, Ahmad Fanindi1, Sajimin1, and Endang Sutedi1 1 Research Center for Animal Husbandry, National Reseacrh Center and Innovation Agency Email : herdiawanmaliq[at]gmail.com Abstract The study was conducted in Babulu District, North Penajam Paser Regency, East Kalimantan, on acid soil (pH = 3.77), with dusty sandy soil texture. The land was not cultivated but only cleared and experimental plots were made, with a dose of 10 tons/ha of manure and 4 tons/ha of limestone. Three cultivars of Bengal grass, namely Panicum maximum cv Riversdale, Panicum maximum cv Hammil, and Panicum maximum cv Purpleguinea, were planted as intercrops between 15 year old palm trees with light intensity of 255.79 mol/m2/sec equivalent to 75% shade intensity, each plot. measuring 6x15 m2, and a spacing of 1x1 m2. The research design used a Randomized Block Design (RAK), and each treatment received 10 replicate plots. The observed variables were biomass production, leaf, stem, leaf/stem ratio, and forage quality (CP, CF, energy, Ca, and P). The results showed that fresh biomass production, leaf production, and leaf/stem ratio of Panicum maximum cv Hammil significantly (P<0.05) were the highest compared to Panicum maximum cv Riversdale, and Panicum maximum cv Purpleguinea. However, the production of fresh stems of Panicum maximum cv Hammil was not significantly different from that of P. maximum cv Purpleguinea. Crude protein content, energy, crude fiber, Ca and P Panicum maximum cv Riversdale and Panicum maximum cv Hammil were not significantly different, while with Panicum maximum cv Purpleguinea were significantly different. Keywords: productivity, Benggala grass, shade, palm oil Share Link | Plain Format | Corresponding Author (Iwan Herdiawan Herdiawan)

102	Grassland Science	ABS-33
POTENTIAL OF UNDERSTOREY VEGETATION IN OIL PALM PLANTATION AS FORAGE SOURCE FOR BEEF CATTLE FEED IN MUSIBANYUASIN, SOUTH SUMATERA Endang Sutedi, Iwan Herdiawan, Yeni Widiawati, Dwi Yulistiani, Diana Andrianita .K. Achmad Fanindi animal husbandry Researh Centre, National Research and Inovation Agency Abstract The study was conducted to determine the potential of understorey vegetation in oil palm plantations as feed source for beef cattle. The study was conducted in Sukadamai Village, Sungai Lilin Subdistrict, Musi Banyuasin District, South Sumatera Province in April representing the rainy season. Plant samples were taken under oil palm tree age at 5, 8 and 11 years old. Sampling area were taken in 1 hectare at each oil palm plantation age, each area were taken randomly 9 samples in size 1 m x 1 m quadrant. Samples obtained were separated to determine its botanical composition, then separated into edible and non edibble. All the sample were weighed to obtain amount of forage production. Light intensity of the palm plantation were also measured. The lower light intensity causes more varieties of weed (narrow and broad leaves) to grow. Production of palatable fresh forage grow under oil palm plantations was 509.78 g/m2, 76.01 g /m2 and, 381.49 g /m2 which is equivalent to 5.15 ton/ha, 2.76 tons/ha and 3.81 tons/ha, respectively for oil palm tree age 5, 8 and 11 years. Potential carrying capacity for cattle weights at 300kg was 3.2 AU /ha, 0.3 AU/ha and 0.58 AU/ha respectively for 5, 8 and 11 years old oil palm tree age. From the results of this study can be concluded that younger oil palm tree age produced more forage biomass for beef cattle feed and have higher carrying capacity. Keywords: oil palm tree, vegetation, forage production, carrying capacity, beef cattle Share Link | Plain Format | Corresponding Author (Endang Sutedi)

103	Grassland Science	ABS-35
Types of Vegetation and Forage Production Natural Pastures in Tarabbi District, Malili Village, East Luwu Regency Sema(a), JA.Syamsu (b), A Ako (c), Rinduwati (d), T.Septiani (e) a)Doctoral Program of agricultural science, graduate school, Hasanuddin University, Makassar, South Sulawesi, Indonesia sema28292[at]gmail.com a)Department of Nutrition and animal feed technology, Faculty of Agriculture, Universitas Puangrimaggalatung, Sengkang, South Sulawesi, Indonesia b,c,d) Faculty of Animal Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia e)Agribussines Study Program, Faculty of Agriculture Universitas Puangrimaggalatung, Sengkang, South Sulawesi, Indonesia Abstract Increasing the productivity of ruminants is of course supported by the availability of forage. The availability of forage in pastures needs to take into account the type of vegetation and the amount of production in the area so that it can meet the needs of livestock. This study aims to determine the types of vegetation and forage production in a natural pasture in Malili village, East Luwu regency. This study used a descriptive/survey method as well as primary data and observations in the field. Measurement types of vegetation and forage production was carried out using the Summed Dominance Ratio method based on frequency, density, and cover area. The results showed that the types of vegetation was dominated by weeds (55%), subsequently of grasses (30%), and then legumes (15%). Fresh weight production were 42.25 tons/ha and 5.20 tons/ha, respectively. Therefore, the condition of natural pastures in Tarabbi District, Malili Village, East Luwu regency, has experienced a decrease in vegetation and forage production Keywords: Grass, Legumes, Forage Production, Pastures, vegetation Share Link | Plain Format | Corresponding Author (Sema sema)

104	Grassland Science	ABS-48
Comparing Indigofera Spp. Vs. Pueraria Spp. In Tropical Dryland Dicky Pamungkas, Alib Shabira Putri, Noor Hudhia Krishna, Ahmad Fanindi, Yenny Nur Anggraeny, Mozart Nuzul Apriliza, and Rina Ariyanti Research Center of Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency of The Republic of Indonesia (BRIN), Cibinong Sciences Center, Jl. Raya Jakarta-Bogor Cibinong, Bogor, 16915 Indonesia. Abstract Legumes are known as green concentrate for ruminants in the tropics and their availability was essential to support livestock productivity. The research was conducted to update the morphology and nutrient quality of four species as fodder crops that grew in lowland agroecosystems (14m above sea level), namely: I. endecaphylla, I.zolingeriana, P. javanica, and P. triloba. Characterization begins with the collection of secondary and primary data, including: soil conditions (soil type, pH, macro and micro nutrients), habitus (origin, growth characteristics, and plant height), morphology (leaves, stems, flowers, fruits, seeds, cuttings/how to grow shoots), production or yield potential, quality (nutritional value and anti-nutritional content). Comparative data was presented descriptively. The results showed that the plants grow in grumosol soil with a pH of H2O 8.2, a pH of KCl 6.9 was slightly alkaline, with 0.17 percent of total nitrogen, 11.0 ppm available P2O5, and 33.08 cmol cation exchange capacity per Kg-1. The four legumes observed were local variety with the typical growth of spread, creeping and upright-branching. Except for I.zolingeriana (tap root), all three varieties have fibrous roots and compound leaves. The leaf width of P. javanica and P. triloba (22.7cm and 34.0cm) was higher than that of I. endecaphylla and I.zolingeriana (4.3cm and 11.3cm). The results of proximate analysis showed that I.zolingeriana had the highest Crude Protein content (percent) (22.0), followed by P. triloba (18.6), P. javanica (18.2), and I. endecaphylla (15.5). While the highest Neutral Detergent Fiber content (percent) was found in P.triloba (59.5), followed by I.zolingeriana (57.4), P. Javanica (57.4), and I. endecaphylla (49.9). The four legumes had secondary metabolites. P. javanica showed the highest concentration of total Alkaloids, Saponins, Terpenoids, Tannins, Phenolics, and total Flavonoids which ranging from 73.5 to 3685.5 ppm. However, the four legumes were safely Keywords: Key words: legumes, morphology, Indigofera spp., Pueraria spp, tropical dry land Share Link | Plain Format | Corresponding Author (Dicky Pamungkas)

105	Grassland Science	ABS-53
Characterization of Local Forage Crop in The Coastal Region of Lebak-Banten Achmad Fanindi, Sajimin, Endang Sutedi, Iwan Herdiawan, Harmini Harmini, Baehaki, Agus Setiawan, Ihat Solihat Research Center for Animal Husbandry, National Research and Innovation Agency Cibinong Science Center, Jalan Raya Jakarta - Bogor, Cibinong Kab. Bogor 16915 Abstract The coastal region of Lebak-Banten is home to 30% of the total buffalo population in the Lebak district. To develop the buffalo population in the area, forage plants (TPT) need to be available year-round. However, the coastal region is categorized as a marginal area due to its limitations such as high salinity and partial acidity. Developing TPT in the coastal area involves introducing superior, salinity or acidity tolerant TPT, or increasing production of local TPT through cultivation techniques. Research was conducted using a survey method based on distance from the beach (25-100 m, 1 km, and 5 km), reflecting the relocation habits of farmers during both dry and rainy seasons. The results of the exploration were then planted in the Ciawi experimental garden using a randomized block design with 3 repetitions. Cultivated grass was also observed, and the parameters measured were productivity and nutritional value of the forage. Results showed that 15 types of TPT could be grown on acidic land, and 5 types on saline land. Axonopus grass had the highest production on a 1m2 area, while Illat Kerbau grass had the highest production per clump. In the saline land, Jotang grass (1238.33 gr/m2) and Japanese grass (584.40 gr/m2) had the highest productions. The highest production after planting was achieved with Binuamgeun grass (38.77 gr/clump) and Benggala cv Mombasa grass (2846.7 gr/clump) for local and cultivated grasses, respectively. These results demonstrate the potential for developing local grass varieties tolerant to salinity or acidity through breeding or cultivation techniques to achieve high production. Keywords: coastal, buffalo, forage, local varieties, saline Share Link | Plain Format | Corresponding Author (Achmad Fanindi)

106	Grassland Science	ABS-63
Adaptation test on growth and biomass production of Brachiaria ruzisiensis mutants in lowland and upland agroecosystems Yenny Nur Anggraeny1a, Pritha Kartika Sukmasari, Alif Shabira Putri , Risa Antari, Noor Hudhia Krishna, Mariyono, Dicky Pamungkas, Setiasih,Wahidin Teguh Sasongko, Farida Yuliant, Wardi, Sigit Puspito, Bambang Haryanto, Dyah Tuwi Ramsiati1, Wahyuni Indah Wulansari, Muchamad Chanafi, Angga Maulana Firmansyah National Research and Innovation Agency, Research Center for Animal Husbandry large ruminant agricultural instrument standardization agency National Research and Innovation Agency, Research Center for Horticulture and Plantation National Research and Innovation Agency, Research Center for Sustainable Development Systems and Life Cycle Assessment 5National Research and Innovation Agency, Research Center for food technology and process Abstract Abstract. Increasing the productivity of Forage Animal Feed can be done through the formation of new superior varieties of Forage Animal Feed. Mutation induction through the use of gamma rays has been widely used to increase plant diversity and productivity. The purpose of this study was to test the growth adaptation and biomass production of Brachiaria ruziziensis mutants through gamma irradiation technology in lowland and highland agro-ecosystems. Adaptation tests were carried out on two mutants of Brachiaria ruziziensis which had been irradiated at doses of 10 Gy (BR1) and 15 Gy (BR 2) and as a comparison, germplasm grass (K) Brachiaria ruziziensis was planted. The adaptation test was carried out in Beji Village, Junrejo District, Batu City and in Alas Tlogo Village, Lekok District, Pasuruan Regency. This study used a factorial randomized block design with treatment 1) agro-ecosystem types, namely lowland and highland and 3 grasses tested, namely K (control), BR 1 and BR 2. Parameters observed were plant height, number of tillers, number of leaves, leaf width and length of leaves, biomass production at 2 months of age. The number of tillers in the highlands was more (P=0.000) than in the lowlands- although the number of tillers is affected by the interaction of mutants and agroecosystems. Plant height in BR 1 and BR 2 was taller (P=0.000) than K- plants have been taller in the lowlands than the highlands (P=0.000). The number of leaves in the lowlands were more (P=0.000) than in the highlands- BR1 and BR2 produced plants with more leaves (P=0,016) than K. BR1 and BR2 are significantly wider (P = 0.000) and longer (P = 0.003) than K. Biomass production in the lowlands was 29,750 tons while in the highlands was 13.9 tons- BR1 produces higher biomass (23,7 ton) than BR2 (20,11 ton) and K (21,8 ton). Keywords: Superior forage, productivity, Brachiaria ruzisiensis and mutants. Share Link | Plain Format | Corresponding Author (Yenny Nur Anggraeny)

107	Grassland Science	ABS-65
Production and Quality of Forage Natural Pastures in the Lompo Tengah Village of Tanete Riaja District, Barru Regency Darmawati1, a), Rinduwati2, b*), A.Ako2,c, Sema3,4 d), and T.Septiani 5,e) 1) Graduate student of Animal Science and technology, Hasanuddin University, Makassar, South Sulawesi, Indonesia 2)Faculty of Animal Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia *rinduwatit[at]yahoo.com 3)Doctoral Program of agricultural science, graduate school, Hasanuddin University, Makassar, South Sulawesi, Indonesia 4)Department of Nutrition and animal feed technology, Faculty of Agriculture, Universitas Puangrimaggalatung, Sengkang, South Sulawesi, Indonesia 5) Faculty of Agriculture Universitas Puangrimaggalatung, Sengkang, South Sulawesi, Indonesia Abstract The aim of the study was to determine the production of dry matter forage and the levels of Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) in natural pastures. This research method uses a descriptive analysis method for forage production which describes the condition of the pasture. Measurement of botanical composition using the ^Actual Weight Estimate^ method using a measuring quadrant of 1 m x 1 m. For forage quality using the vansoets analysis method. The data taken in this study are primary data and secondary data. Primary data is obtained from direct measurements in the field, while secondary data comes from literature and related institutions. The type of primary data taken is forage production based on existing production data. The data obtained were tabulated and calculated to obtain the total botanical composition. The results showed that the natural pastures of Lompo Tengah Village Tanete Riaja District, Barru Regency had a dry matter production of 7.35 tonnes-1. The average content of NDF is 68.02% and ADF is 44.20%. The conclusion that can be drawn is that the area of natural pastures in the Lompo Tengah village Tanete Riaja District, Barru Regency has experienced a decrease in production because the pastures have been overgrazed and the NDF content is quite good, but the ADF content is still relatively high. Keywords: Production, Forage, NDF, ADF, Natural Pastures Share Link | Plain Format | Corresponding Author (Rinduwati Rinduwati)

108	Grassland Science	ABS-71
Gamma-ray irradiation was used to make the Bengal grass variety cv Mombasa tolerant of shade. Sajimin, Harmini, Ahmad Fanindi, Endang Sutedi, Iwan Herdiawan, Dwi Yulistiani, Asep Mulyadi, Karya, BRIN Abstract This research aims to assess the radiosensitivity of Bengal grass cv Mombasa and the influence of the M1V1 generation on the shade-tolerant assembly of Bengal grass cv Mombasa. The study was conducted at the experimental garden of the Indonesian Research Institute for Animal Production (IRIAP), Ciawi, Bogor in 2022, using a paired T-test design for Bengal grass cv Mombasa resulting from gamma-ray irradiation at 100, 200, 300, 400, 500, 600, 700, and 800 Gy with shade treatment (50%) and without shade (open cultivation). Observed Bengal cv Mombasa parameters were agronomic performance and grass productivity. Radiation treatment had a significant influence (P 0.05) on plant height, leaf length, lower leaf length, lower leaf width, number of shoots, and stem weight, but not on stem height, leaf width, internode length, internode diameter, panicle length, number of flowers, or total weight. Four hundred Gy of gamma irradiation resulted in the greatest plant height, while 100 Gy of gamma irradiation resulted in the greatest plant mass. Keywords: diversity, forage, irradiation Share Link | Plain Format | Corresponding Author (Harmini Harmini)

109	Grassland Science	ABS-122
Relative Feed Value of Leguminous Plants Grown on Marginal Land Rinduwati1, Rohmiyatul Islamiyati1, a), Budiman Nohong1, Indrawirawan1, Rahmawati Semaun2, Nurjaya3 Rinduwati1, Rohmiyatul Islamiyati1, a), Budiman Nohong1, Indrawirawan1, Rahmawati Semaun2, Nurjaya3 1Faculty of Animal Science, Hasanuddin University, Makassar. 2Muhammadiyah University, Parepare 3Puangrimanggalatung University, Sengkang a) E-mail: islamiyati[at]unhas.ac.id. Abstract Planting legumes on marginal land as a source of quality feed can increase livestock productivity. The purpose of this study was to determine the relative feed value (RFV) of corn straw, peanuts, mung beans and soybeans from marginal land. The results showed that the crude protein content of peanut straw was significantly (P<0.05) higher than that of mungbean straw and very significantly (P<0.01) higher than that of soybean straw and corn straw. The NDF and ADF content of peanut and mung bean straw were not significantly different (P>0.05), but lower than the NDF and ADF content of soybean straw and corn straw, while the NDF and ADF content of soybean straw were not significantly different (P >0.05) with corn straw. The dry matter digestibility of peanut, mung bean and soybean straw were not significantly different (P>0.05), but the three were significantly different (P<0.05) higher than the dry matter digestibility of corn straw. The dry matter intake of peanut and mung bean straw was not significantly different (P>0.05), but higher than the dry matter intake of soybean straw and corn straw, while the dry matter intake of soybean straw was not significantly different (P>0. 05) with the intake of corn straw dry matter. The relative feed values (RFV) of peanut and mung bean straw were not significantly different (P>0.05), but higher than the relative feed values of soybean straw and corn straw, while the relative feed values of soybean straw were not significantly different (P>0, 05) with the relative feed value of corn straw. It was concluded that the highest relative feed value (RFV) was peanut mung bean, soybean and corn straw. Keywords: legumes, maize, marginal land, quality, relative feed value Share Link | Plain Format | Corresponding Author (Rohmiyatul Islamiyati)

110	Grassland Science	ABS-144
THE PHYSIOLOGICAL RESPONSE of MUTANT 1 (M1) Indigofera zollingeriana UNDER DIFFERENT GAMMA IRRADIATION Marhamah Nadir, Ade Irma Rusiana, Rinduwati, Budiman Laboratorium Forages, Faculty Animal Husbandry, Universitas Hasanuddin Abstract Indigofera zollingeriana is a forage for animal feed that is able to grow and develop in abiotic stress conditions such as salinity, drought, low nutrient and heavy metal contaminated land. Gamma-rays irradiation is an effort to enhance the genetic diversity. It is assumed that genetic changes will trigger and activate the changes in the composition of amino acids so that physiological ability to withstand stress will increase. The objective of the study was to determine the influence of the dose of gamma irradiation on the morphological and physiological responses of Indigofera leaves which consisted of the changes of the leaf area, stomata, chlorophyll content and proline enzyme analysis. The experiment was conducted in randomized complete block design (CRD) with 5 treatments and 6 replications, so that there were 30 experimental units. Gamma irradiation was treated with a dose respectively of 0 Gy, 50 Gy, 100 Gy, 150 Gy and 200 Gy. Samples were taken at the planting of Indigofera Moncongloe Lappara Maros Regency, the age of the plants were similar and the leaves were taken after pruning the plants for 60 days. The data were processed using SPSS 16.0 software. The results showed that the dose of gamma irradiation had a significant effect on the number of stomata and chlorophyll content (P <0.05), while the dose of irradiation had no significant effect (P>0.05) on leaf area and the concentration of proline enzyme. Keywords: Indigofera zollingeriana, gamma irradiation, leaf area, number of stomata and chlorophyll content, proline. Share Link | Plain Format | Corresponding Author (marhamah nadir)

111	Grassland Science	ABS-167
The use of plant explants to obtain embryogenic callus clams as an early stage in the selection of Napier Grass cv Taiwan Ali Husni, Mia Kosmiatin, Harmini Harmini BRIN Abstract The purpose of this study was to obtain 50-100 clams from a population of embryogenic callus from Taiwan elephant grass cultivars. The study was divided into three stages 1) sterilization, which was completed by immersing the material in 70% alcohol solution, 20% chlorox, and rinsing with sterile distilled water so that it was ready to be cultured in the treatment media- 2) callus induction with explants of young leaves in culture medium supplemented with growth regulator (PGR) 2,4-D at a concentration of 2 mg/l and enriched with amino acids and casein hydrolyzat. 3) callus proliferation and regeneration using culture-based media, namely full MS plus growth regulators (ZPT) enriched with 0.3 mg/l BA and 5-Methyl-tryptophan as well as amino acids and casein hydrolyzate, while regeneration into plants such as through adventitious shoot formation or somatic embryogenesis with a method of adding 0.5 g of callus to each bottle that already contains the treatment medium. The results showed that its success rate of explant sterilization was 10-78%, and the ability of tissue to form callus ranged from 60-80%, with the highest callus-forming ability obtained from culture explants with chemical and physical combination sterilization by burning, while at the proliferation stage it was obtained from 5 times per series on average. After the second week, the average callus weight was 1.72-1.76 g callus/bottle, enhancing to 1.92-2.07 g callus/bottle for a total of 150 bottles, with callus cells capable of regenerating into shoots through the use of organogenesis or embryogenesis reaching 78.1 - 85.3%. The initial selection method for fodder plants from explants to obtain callus was concluded to have potential to be further developed. Keywords: selection, induction, proliferation, grass, callus Share Link | Plain Format | Corresponding Author (Harmini Harmini)

112	Grassland Science	ABS-170
Nitrogen Utilization in Decoupled Aquaponic Systems: Exploring the Nitrification Process and Considerations for Effective Implementation Abdel Razzaq Al Tawaha1*, Puteri Edaroyati Megat Wahab1, Hawa binti Jaafar1, Ali Tan Kee Zuan2, Mohd Zafri Hassan3, Abdel Rahman M. Al-Tawaha4, Zulkifli Maulana5,Lellah Rahim6 1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 2Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 3Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 4Department of Biological Sciences, Al-Hussein Bin Talal University, Maan, Jordan 5 Department of Agrotechnology, Faculty of Agriculture, Bosowa University, Makassar, 90245, Indonesia 6Departmen of livestock production ,Faculty of Animal Science,Hasanuddin University Abstract The nitrification process is a crucial step in decoupled aquaponic systems as it converts toxic ammonia, produced from fish waste, into nitrate, a form of nitrogen readily available for plant uptake. This paper investigates the advantages of utilizing a decoupled aquaponic system, focusing on efficiently using nitrogen derived from the waste generated in the fish tank for plant uptake. The nitrification process involved in this system is described in detail, emphasizing the role of nitrifying bacteria. This approach^s potential risks, drawbacks, and disadvantages are discussed to provide a comprehensive understanding of its implementation. Keywords: Specific surface area, Nitrobacter, Nitrosomonas, biofilter tank, aquaponics Share Link | Plain Format | Corresponding Author (sitti nurani sirajuddin)

113	Halal and Functional Food	ABS-49
Enhancing the Quality of Honey Powder Production Through Vacuum Foam Drying Optimization Dodyk Pranowo, Zahrotul Firdausi Agroindustrial Technology Department Faculty of Agricultural Technology Brawijaya University Abstract This study aimed to enhance the quality of honey powder production through the optimization of the vacuum foam drying method. Honey powder is a popular product that is rich in various compounds, including essential and non essential amino acids, vitamins, and minerals. The vacuum foam drying method is commonly used to dry heat sensitive, sticky, and viscous materials without altering their quality. The study employed response surface methodology (RSM) with a central composite design (CCD) to investigate the effects of drying temperature and time on the porosity, hygroscopicity, color, and antioxidant activity of honey powder. The experiment involved drying temperatures of 65oC, 70oC, and 75oC, and drying times of 5, 6, and 7 hours. The results showed that the optimal drying temperature and time were 75oC and 5 hours, respectively. Honey powder produced at this condition showed a porosity value of 0.37%, hygroscopicity of 0.02%, color coordinates of 1.03, and antioxidant activity (IC50) of 91 ppm. These findings suggest that optimizing the vacuum foam drying method could enhance the quality of honey powder production, leading to better products for consumers Keywords: honey powder - Vacuum Foam Drying, Central Composite Design Share Link | Plain Format | Corresponding Author (Dodyk Pranowo)

114	Halal and Functional Food	ABS-50
The effects propolis and royal jelly on the VEGF expression in the healing of traumatic ulcers of the diabetes mellitus (in vivo) Miftakhul Cahyati1, Dewi Masyitho2*. Agus Susilo3, Agustine Hanafi Putri1, Viranda Susanti1, Salsa Al Fitri Nur Milenia4 1Department of Oral Medicine, Faculty of Dentistry, Brawijaya University, Malang, 65145, East Java, Indonesia 2Faculty of Animal Husbandry, University of Islam Malang, Malang 65144, East Java, Indonesia 3Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, East Java, Indonesia 4Faculty of Dentistry of Brawijaya University, Malang, 65145, East Java, Indonesia Abstract Background: Diabetes mellitus (DM) can be defined as a chronic metabolic disorder due to insufficiency of insulin function and developing vascular complications. The most common lesion in the oral cavity is an ulcer, which is a loss of the epithelial lining of the oral mucosa so that it exceeds the basal membrane and reaches the lamina propria. Propolis is a natural substance that exhibits antimicrobial, anticancer, antifungal, antiviral, and anti-inflammatory properties. In addition, royal jelly has various biological activities, such as insulin-like and antioxidant functions, that can reduce microvascular complications (retinopathy, neuropathy, and nephropathy) caused by diabetes mellitus. Objective: To determine the effect of propolis and royal jelly on VEGF expression in wound healing of the oral mucosa in an experimental animal model of diabetes mellitus rats. Methods: A total of 6 white Wistar rats in each group, namely the control group, propolis group, royal jelly group, and mixed group, were given streptozotocin then performed ulcer induction, and provided treatment according to the group by intragastric sondation. The rats were then decapitated using the cervical dislocation technique on their necks on days 3, 5, and 7. Results and Discussion: The results of this study showed an increase in the amount of VEGF expression in the treatment group, although there was no statistically significant difference. This may be caused by the concentration or dosage of the active substances contained in propolis and royal jelly, ulcer induction, and the method of drug administration. Conclusion: There is no significant difference between the administration of propolis and royal jelly on the healing of oral mucosal wounds in experimental animal models of diabetes mellitus rats. Keywords: Diabetes Mellitus- Propolis- Royal Jelly- Traumatic Ulcer- VEGF Share Link | Plain Format | Corresponding Author (Miftakhul Cahyati)

115	Halal and Functional Food	ABS-157
Animal fats identification using raman spectroscopy D. Afdal, N.S. Aminah, M. Djamal Institut Teknologi Bandung Abstract Food of the highest quality and safety is a basic requirement for human life. However, we live in the age of globalization, which brings rapid changes to people, cultures, economies, societies, and the environment. These changes include global alerts, international trade regulations, and non-tariff barriers. These factors affect food security and sustainability. Food safety requires reliable procedures that guarantee the animal fat^s origin. In the present work, we attempt to verify the effectiveness of using polymorphic fat characteristics to distinguish animal fat origins. We use Raman spectroscopy to detect the structural information about fat crystals. It is shown that a single Raman band at 1421 cm-1 distinguishes pork fat from beef fat. This band is characteristic of the &#946-^ polymorphism of fats. Pork fats show this band because they contain the &#946-^ polymorph after rapid cooling at room temperature. In beef-pork fat mixtures, this band is not detected even at 50% pork fat- the addition of beef fat to pork fat is likely to result in a mixed fat with a completely different polymorphic behavior. This method appears to detect beef products contaminated with pork fat. Keywords: Raman spectroscopy- pork fat- polymorphic- beef fat Share Link | Plain Format | Corresponding Author (Nina Aminah)

116	Poultry Production and Nutrition	ABS-3
The Effect of Dietary Nano Zinc Phytogenic (NZP) on Intestinal Villus and Intestinal E. coli Bacteria Population of KUB Chickens Fed Low-High Nutrient Diet C Hidayat1*, E Wina2, R Krisnan1, H Zainal1, T Cahyaningsih2, SA Asmarasari1, YA Hoesen3, Isbandi1, M Hamdan1, A Suhandi1 1 Research Center for Animal Husbandry, National Research and Innovation Agency, 2 Indonesian Research Institute for Animal Production, Ciawi Bogor 16720, Indonesia 3 Research Center for Appropriate Technology, National Research and Innovation Agency Abstract Abstract is submitted as file Keywords: Nano zinc phytogenic, KUB chicken, villus, E.coli, intestines Share Link | Plain Format | Corresponding Author (Cecep Hidayat)

117	Poultry Production and Nutrition	ABS-85
EFFECTS OF THE ADDITIONAL CALCIDIFIER AS FEED ADDITIVE ON THE PERFORMANCE, ABDOMINAL FAT, INTERNAL ORGANS OF QUAIL, AND INTERNAL, EXTERNAL QUALITY OF QUAILS EGG Sjofjan O., Natsir M.H., Nuningtyas Y.F., and Adli D.N Animal Nutrition and Feed Department, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract This research used 200 quails Coturnix coturnix japonica. The research method used was a field experiment using a Completely Randomized Design (CRD) with 5 treatments and 5 replications, in every replication 8 quails respectively. The treatments in this research were T0: basal feed, T1: basal feed + 0.25% calcidifier, T2: basal feed + 0,5% calcidifier, T3: basal feed + 0,75% calcidifier, T4: basal feed + 1% calcidifier. The variables observed were performance, percentage internal organ, abdominal fat quail, and internal, external quality of quail^s egg. The data were analyzed by Analysis of Variance (ANOVA) and Duncan^s Multiple Range Test (DMRT). The results showed that the use of a calcidifier had no significant effect (P>0,05) on the feed consumption, feed conversion ratio, mortality, income over feed cost, the disposition of meat, abdominal fat, the percentage of internal organs (gills, heart, liver, spleen), on the egg weight, length egg, width egg, thickness egg, index egg, egg white yolk volume, egg yolk color, and specific gravity. However had a significant difference (P<0,05) to the egg white and yolk viscosity. Then, it had a very significant effect (P<0,01) on egg production. It can be concluded that calcidifier in a feed additive did not have significant effect on feed consumption, feed conversion ratio, mortality, income over feed cost, the disposition of meat, abdominal fat, the percentage of internal organs (gills, heart, liver, spleen), on the egg weight, length egg, width egg, thickness egg, index egg, egg white yolk volume, egg yolk color, and specific gravity. However, it can affect egg production and affect the external quality of the quail egg. Keywords: calcidifier, feed additive, performance, internal organs, quail egg Share Link | Plain Format | Corresponding Author (Osfar Sjofjan)

118	Poultry Production and Nutrition	ABS-86
Preferences of black soldier fly larvae through different substrate on broiler chickens: A meta-analysis Sjofjan O., Natsir M.H., Nuningtyas Y.F., and Adli D.N Animal Nutrition and Feed Department, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract A meta-analysis was conducted to determine preferences of black soldier fly larvae through different substrate on broiler chickens. A dataset was constructed based on relevant published papers. An algorithm was constructed with a search in Scopus, Web of Science, Pub Med, and Medline using the MESH terms ^chicken^, ^substrate^, ^broiler chicken^, ^performance^, and ^black soldier fly^. After carefully evaluation, the final dataset consisted 10 in-vivo studies comprising 35 treatments units. The data analysis and coding were performed using software R version 4.2.1 ^Funny-looking kid^ computing with library mode (cowplot)- (tidyverse)- and (viridis)- and (nlme). Our meta-analysis with regard to growth performance, the different source of substrate did not affect the body weight, FCR, body weight gain (BWG), final body weight (FBW), and feed intake (FI) (p > 0.05). In conclusion, the different substrate on rearing black soldier fly didn^t directly affect to the growth performance of broiler chicken Keywords: body weight, broiler chicken, black soldier fly, performance, substrate Share Link | Plain Format | Corresponding Author (Osfar Sjofjan)

119	Poultry Production and Nutrition	ABS-87
The effects of essential oil as an additive produced from black soldier flies (Hermetia Illucens) on broiler chickens: A meta-analysis Sjofjan O., Tistiana H., and Adli D.N Animal Nutrition and Feed Department, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract A meta-analysis was conducted to The effects of essential oil produced from black soldier flies (Hermetia Illucens) on broiler chickens: A meta-analysis. A dataset was constructed based on relevant published papers. An algorithm was constructed with a search in Scopus, Web of Science, Pub Med, and Medline using the MESH terms ^additive^, ^broiler chickens^, ^growth performance^, ^pupa^, and ^black soldier fly^. After carefully evaluation, the final dataset consisted 9 in-vivo studies comprising 35 treatments units. The data analysis and coding were performed using software R version 4.2.1 ^Funny-looking kid^ computing with library mode (cowplot)- (tidyverse)- and (viridis)- and (nlme). Our meta-analysis with regard to growth performance, the different source of substrate did not affect the body weight, FCR, body weight gain (BWG), final body weight (FBW), and feed intake (FI) (p > 0.05). In conclusion, effects of essential oil as an additive produced from black soldier flies (Hermetia Illucens) on broiler chickens didn^t directly affect to the growth performance of broiler chicken. Keywords: body weight, broiler chicken, black soldier fly, essential oil, performance Share Link | Plain Format | Corresponding Author (Osfar Sjofjan)

120	Poultry Production and Nutrition	ABS-96
Analysis of Natural Honey Oligosaccharides Activity as a Source of Prebiotics In Vitro For Poultry Muhammad Daud (a*), M.Aman Yaman (a), Zulfan(a), Zahrul Fuadi (b) a) Animal Husbandry Departement, Faculty of Agriculture, Syiah Kuala University Banda Aceh 23771. Indonesia (b) Animal Husbandry Departement, Faculty of Agriculture, Abulyatama University, Aceh Besar. Indonesia Abstract This study aims to determine lactic acid bacteria^s (LAB) ability to use natural honey for growth and function as a source of prebiotics for poultry. Natural honey and LAB, including B. bifidum, B. animalis, L. casei Rhamnosus, and L. bulgaricus, as well as pathogenic bacteria, including E. coli and Salmonella, were tested in vitro. The ability of natural honey to serve as a source of prebiotics was tested on LAB growth. Furthermore, the MRS base liquid media was used as the growth media for LAB, but the glucose medium was replaced with natural honey oligosaccharides. MRS base media without a sugar component was used in the control group. Cultures from each LAB were grown on the media containing natural honey. Calculation of the number of LAB was conducted after incubation for 24 hours. The same process was performed for the growth media of pathogenic bacteria, namely E. coli and Salmonella. The growth media for E. coli bacteria consists of Lab lemco powder, Yeast extract, Peptone, Sodium chloride, Agar, and natural honey, while Salmonella consisted of Beef extract, Peptone, Disodium phosphate, Ferrous sulfate, Bismuth Sulfite indicator, Brilliant green, Agar, and natural honey. Variables observed include natural honey^s oligosaccharide content, LAB^s growth and pathogenic bacteria. The data obtained were analyzed descriptively using ANOVA. The results showed that the oligosaccharide content of natural honey obtained consisted of Fructose (38.20%), Dextrose (31.30%), Maltose (7.30%), and Sucrose (1.30%). Meanwhile, the growth of LAB on media indicates that B. bifidum, B. animalis, L. casei Rhamnosus, and L. bulgaricus could use natural honey as a growth medium. Pathogenic bacteria showed a decrease in the population of E. coli and Salmonella bacteria. Therefore, natural honey contains oligosaccharides (fructose, dextrose, maltose and scrose) and can increase the growth of lactic acid bacteria and can inhibit the growth of pathogenic bacteria (E. coli and Salmonella. Keywords: Natural honey, Poultry, Prebiotic, Oligosaccharides Share Link | Plain Format | Corresponding Author (Muhammad Daud)

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