Immune response (antibody titers and leukocyte profile) of local chickens given in ovo butyric acid Rantan Krisnan, Cecep Hidayat, Mohammad Ikhsan Shiddieqy, Endang Sutedi, Iwan Herdiawan, Tuti Haryati, Rusdiana Supardi, Yuli Retnani, M. Hamdan, Andi S
Research Organization for Agriculture and Food, National Research and Innovation Agency
Abstract
The aim of this study was to determine the immune response of local chickens given butyric acid through the in ovo technique. There were five in ovo material treatments, namely- T1 (admix sodium butyrat), T2 (pure butyric acid), T3 (antibiotic), T4 (Phosphate-buffered saline / Sham), and T5 (uninjected). All ingredients were dissolved in 0.5 ml PBS every injection at a dose of 1 kg/ton butyric acid.The injection process is carried out on the amnion while 18 days of incubation using a 20G needle (diameter 0.9 mm and a length 8 mm). The parameters observed were antibody titers, total and differentiation of leukocytes. The results showed that butyric acid treatment and antibiotic treatment significantly (P<0.05) gave a better effect on antibody titers, lymphocytes and heterophils compared to sham and uninjected treatment. However, it had no significant effect (P>0.05) on total leukocytes and other leukocyte differentiation. There was no significant effect due to the use of different types of butyric acid, so the recommendation of the type of butyric acid chosen will depend on the consideration of economic aspects and its availability.
Keywords: in ovo, butyric acid, antibody titer, leukocyte, local chicken.
