THE PHYSIOLOGICAL RESPONSE of MUTANT 1 (M1) Indigofera zollingeriana UNDER DIFFERENT GAMMA IRRADIATION Marhamah Nadir, Ade Irma Rusiana, Rinduwati, Budiman
Laboratorium Forages, Faculty Animal Husbandry, Universitas Hasanuddin
Abstract
Indigofera zollingeriana is a forage for animal feed that is able to grow and develop in abiotic stress conditions such as salinity, drought, low nutrient and heavy metal contaminated land. Gamma-rays irradiation is an effort to enhance the genetic diversity. It is assumed that genetic changes will trigger and activate the changes in the composition of amino acids so that physiological ability to withstand stress will increase. The objective of the study was to determine the influence of the dose of gamma irradiation on the morphological and physiological responses of Indigofera leaves which consisted of the changes of the leaf area, stomata, chlorophyll content and proline enzyme analysis. The experiment was conducted in randomized complete block design (CRD) with 5 treatments and 6 replications, so that there were 30 experimental units. Gamma irradiation was treated with a dose respectively of 0 Gy, 50 Gy, 100 Gy, 150 Gy and 200 Gy. Samples were taken at the planting of Indigofera Moncongloe Lappara Maros Regency, the age of the plants were similar and the leaves were taken after pruning the plants for 60 days. The data were processed using SPSS 16.0 software. The results showed that the dose of gamma irradiation had a significant effect on the number of stomata and chlorophyll content (P <0.05), while the dose of irradiation had no significant effect (P>0.05) on leaf area and the concentration of proline enzyme.
Keywords: Indigofera zollingeriana, gamma irradiation, leaf area, number of stomata and chlorophyll content, proline.
