Development and characterization of bio-degradable Annona-based film inclusion with chitosan (a) Institute of Tropical Aquaculture and Fisheries Abstract Biofilm is a biodegradable and sustainable packaging material. In contrast, synthetic packaging has raised serious environmental concerns by their non-biodegradability. The aim of this study is to develop a new natural-based biofilm made of Annona extract reinforced with chitosan. Annona-based biofilms were prepared using different concentrations of chitosan (0, 1, 2 and 4%). The effect from inclusion of different concentration of chitosan were evaluated using physical, mechanical, chemical and optical properties of the film. Thickness values for all biofilms showed significant difference (p<0.05) to each other. The film thickness was significant increase with increasing chitosan concentration due to compacity of film network. Biofilm without chitosan showed significant lower (p<0.05) moisture and elongation at break (EAB) compared to biofilm with 1% chitosan. Meanwhile, the opacity values of biofilm without chitosan showed significant lower (p<0.05) compared to biofilm with 4% chitosan. The films with higher chitosan were less transparent compared than the control due to the presence of more compounds in the film. Increasing chitosan concentration also resulted in lighter brown-yellowish color films as compared to deep brown control films. Overall, biofilm inclusion with Annona extract and chitosan has potential for natural biodegradable packaging material. Keywords: Annona, Chitosan, Biofilm Topic: Sustainable Aquaculture and Fisheries |
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