Application of whole cell microalgae as an alternative to prevent vibriosis
E. Susianingsih1, I.A.K. Kadriah1 Nurhidayah1

National Research and Innovation Agency

Abstract

Abstract.The more intensive aquaculture activities make the development of the disease increase. One of them is vibriosis, a disease caused by the pathogenic Vibrio bacteria. Several alternative disease prevention has been carried out. One of them is by using microalgae as biocontrol to control the growth of pathogenic bacteria. Naturally, bacteria and plankton (phytoplankton and zooplankton) interact with each other in aquatic ecosystems. In clean water conditions, good bacteria and plankton can grow in abundance. However, if the water quality getting worst, the pathogenic bacteria have the opportunity to grow better. This study aims to determine the effect of using whole cell microalgae on the growth of pathogenic bacteria. Using 5 types of microalgae namely: Melosira sp, Phaeodactylum sp, Nannochloropsis sp, Porphyridium aerugenum and Porphyridium S with a density of 105 cells/mL. Vibrio harveyi for challenge test was used with a density of 107 CFU/mL. In vitro challenge test was carried out using a completely randomized design with 6 treatments. Whereas in vivo it was carried out using PL 16 tiger shrimp at a density of 20 individuals/20 L volume. The results showed that in vitro administration of whole cell microalgae types P.aerugineum, Nannochloropsis sp and Melosira sp could inhibit the growth of pathogenic Vibrio bacteria, whereas in vivo administration of microalgae Porphyridium aerugineum and Phaeodactylum sp was able to inhibit the growth of pathogenic Vibrio bacteria better than other types of microalgae and control.

Keywords: vibriosis, microalgae, challenge test, pathogenic vibrio,

Topic: Sustainable Aquaculture and Fisheries