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1	Animal Agribusiness and Related Subjects	ABS-32
Designing a Community-Based Business Model for Pelleted Horse Feed Using Local Raw Materials in Lamphun Province, Thailand Worapong Yongyot(a), Nathitakarn Phayakka(b), Daranrat Jaitiang(b), Saowaluck Yammuen-art(a*) a)Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand, 50200 *saowaluck.y[at]cmu.ac.th b)Department of Agricultural Economy and Development, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand, 50200 Abstract This study designs a community-based business model for pelleted horse feed produced from locally available raw materials in the horse-breeding community of Ban Thi District, Lamphun Province. A quantitative approach was employed through experimental feed formulation using ground maize, rice bran, and soy sauce by-product meal, followed by pelleting and evaluation of cost efficiency and feed quality. Analytical results indicate value creation across products, service, human resources, and brand image, with the model structured according to the nine components of the Business Model Canvas. The pelleted feed is nutritionally balanced and safe, and a direct farm delivery service is feasible within existing breeder networks. Packaging that preserves quality and palatability supports a consistent customer proposition. Cost-benefit comparison shows a reduction in horse-raising costs of approximately 10-20% relative to commercial feed, suggesting economic viability at community scale. The proposed value chain enhances local agribusiness capacity and farmer income while supporting the long-term sustainability of the horse-breeding sector. The study bridges animal nutrition and agribusiness by demonstrating how localized feed formulation and organized distribution are integrated into a coherent business model, providing a practical prototype for livestock-based rural development. Keywords: pelleted horse feed, equine nutrition, agribusiness, value chain, Thailand Share Link | Plain Format | Corresponding Author (Worapong Yongyot)

2	Animal Agribusiness and Related Subjects	ABS-69
From Practice to Purity: Unraveling the Psychosocial Pathways from Pro-Environmental Livestock Integration to Reduced Agrochemical Dependence in Coffee Farming Retno Pramnesti (a), Jaisy Aghniarahim Putritamara (a), Priyo Sugeng Winarto (a) Brawijaya University Abstract The transition to sustainable agriculture necessitates understanding the complex drivers of farmer behavior. This study investigates the psychosocial mechanisms through which Green Livestock Action (GLA) reduces tolerance for synthetic agrochemicals within integrated goat-coffee farming systems. Data from 100 farmers in East Java, Indonesia, were analyzed using Structural Equation Modeling-Partial Least Squares (SEM-PLS). The results demonstrate that GLA does not exert a direct effect on synthetic input tolerance. Instead, its influence is fully mediated by a suite of psychosocial factors. Environmental Self-Identity, Eco-Guilt, and Social Norms (both Injunctive and Descriptive) emerged as potent, positive catalysts for pro-environmental behavior. Conversely, Perceived Risk and Constraints significantly inhibited the adoption of green practices. The model accounted for substantial variance in farmer behavior, underscoring the primacy of psychosocial pathways. These findings critically advance the literature by delineating the indirect, psychologically embedded route through which sustainable farming practices effect change. We conclude that interventions aimed at enhancing environmental self-identity, cultivating ecological awareness, and reinforcing positive social norms are paramount for scaling GLA and accelerating the adoption of sustainable integrated farming systems. Keywords: Green Livestock Action, Integrated Farming, Agrochemical Reduction, Psychosocial Mediation, Structural Equation Modeling (SEM-PLS), Sustainable Agriculture. Share Link | Plain Format | Corresponding Author (Retno Pramnesti)

3	Animal Agribusiness and Related Subjects	ABS-70
Driving Adoption of Sustainable Feed Innovations: The Pivotal Role of Product Assessment over Environmental Awareness in Shaping Farmers Willingness to Pay Adinta Nurul Sasikirana (a), Anie Eka Kusumastuti (a), and Jaisy Aghniarahim Putritamara (a*) (a) Department of Socio-economics, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia jaisyap[at]ub.ac.id Abstract Despite the potential of innovative feed additives to enhance livestock productivity and environmental sustainability, their market penetration is often hindered by low farmer adoption. This study addresses a critical gap by investigating the psychological and economic drivers of farmers willingness to pay (WTP) for a novel sustainable feed supplement, AETT Multi-Nutrient Granule. Grounded in a robust theoretical framework, we posit purchase intention as a central mediator between key antecedent familiarity, environmental awareness, and product assessment and WTP. Data were collected from 30 livestock farmers and analyzed using Partial Least Squares Structural Equation Modeling (PLS-SEM). The results demonstrate that product assessment is a powerful direct predictor of purchase intention, which in turn, directly governs WTP. Furthermore, purchase intention fully mediates the relationship between product assessment and WTP. Interestingly, neither familiarity nor environmental awareness significantly influenced intention or WTP, suggesting that in this context, pragmatic product evaluation outweighs general environmental concerns or prior knowledge. These findings offer pivotal insights for businesses and policymakers, highlighting that strategic communication must pivot from creating general awareness to convincingly demonstrating tangible product benefits to drive adoption and support the transition towards more sustainable agricultural practices. Keywords: Willingness to Pay, Sustainable adoption, Product Assessment, Feed innovation, farmers decision-making, agricultural sustainability Share Link | Plain Format | Corresponding Author (Adinta Nurul Sasikirana)

4	Animal Agribusiness and Related Subjects	ABS-72
Determinants of Climate-Smart Agriculture Adoption among Smallholder Dairy Farmers in Indonesia: Evidence from East Java, Indonesia Gilang Nanda Pranadita, Tina Sri Purwanti*, Jaisy Aghniarahim Putritamara Universitas Brawijaya Abstract This study investigates the determinants and extent of dairy farmers^ adoption of Climate-Smart Agriculture (CSA) practices in Ngantang and Pujon Districts, Malang Regency, East Java, Indonesia. Using a quantitative descriptive survey of 200 dairy farmers selected through purposive sampling, the research applies a multivariate probit model to identify socioeconomic and institutional factors shaping adoption behavior. The six CSA practices analyzed include pasture-nutrition management, agroforestry, improved waste management, enhanced feed-conversion efficiency, livestock vaccination, and integrated crop-livestock systems. The results show heterogeneity in adoption across practices: access to extension services strongly increases the likelihood of adopting pasture-nutrition management and livestock vaccination- membership in farmer groups and longer farming experience significantly enhance agroforestry uptake- access to credit and herd size are key for improved waste management- while age, occupation type, and information-seeking activity influence feed-conversion improvements. Formal education and farming experience drive the adoption of integrated crop-livestock systems. In contrast, gender and household size have no significant effects. The majority of respondents are middle-aged men with basic education and more than 10 years of farming experience, managing an average of 5.6 animal units, and most had not received extension support during the previous year. The findings highlight the critical role of extension services, credit access, and collective organization in scaling up CSA adoption within smallholder dairy systems. Strengthening farmers^ technical knowledge, financial inclusion, and group-based learning could accelerate climate-resilient transformation in Indonesia^s dairy sector. Keywords: Climate-Smart Agriculture-dairy farming- technology adoption- multivariate probit-Indonesia Share Link | Plain Format | Corresponding Author (Tina Sri Purwanti)

5	Animal Agribusiness and Related Subjects	ABS-77
Comparative Analysis of Production Performance and Economic Efficiency between Open and Closed House Broiler Systems under a Partnership Model in Malang Regency, Indonesia Nanang Febrianto, Nabila Nur Hidayati, Muhammad Helmi, Puji Akhiroh, Priyo Sugeng Winarto, Budi Hartono* Department of Socio-Economic, Faculty of Animal Science, Universitas Brawijaya, Malang 65145 Indonesia Abstract This study aims to compare the production performance and production costs of broiler farms using open house and closed house systems under a partnership (kemitraan) model in Malang Regency, East Java. Data were collected through a case study approach using farm records, financial reports, direct interviews with farm managers, and field observations. Key performance indicators evaluated included Feed Conversion Ratio (FCR), Body Weight (BW), depletion rate, and Production Index (IP). Economic analysis was conducted based on total fixed and variable costs. The results showed that the closed house system demonstrated superior production performance compared to the open house system. The closed house achieved a lower FCR of 1.51 compared to 1.62 in the open house, and a higher final BW of 2.10 kg versus 2.06 kg. The depletion rate was lower in the closed house (3.1%) than in the open house (3.6%), while the Production Index reached 421.13, exceeding the open house IP of 350.24. Production cost analysis revealed total fixed costs of Rp14,032,082 and variable costs of Rp549,900,000, resulting in a total cost of Rp563,932,082. Feed accounted for the largest portion of variable expenses (72.54%), followed by DOC (21.15%). In conclusion, the closed house broiler farming system under a partnership model in Malang Regency demonstrated better technical efficiency and economic feasibility than the open house system. Enhanced environmental control in closed housing contributed to improved growth performance, reduced mortality, and optimized feed utilization, making it a more profitable and sustainable option for commercial broiler production. Keywords: Broiler, Closed house, Partnership, Production performance Share Link | Plain Format | Corresponding Author (Budi Hartono)

6	Animal Agribusiness and Related Subjects	ABS-78
Determinants of climate change adaptation strategies among beef cattle farmers in East Java, Indonesia Niche Permata Sari1*, Budi Hartono1, Hary Dwi Utami1, Hary Toiba2 1 Socio-Economics, Animal Science Faculty, Brawijaya University, Malang, Indonesia 2 Socio-Economics, Agriculture Faculty, Brawijaya University, Malang, Indonesia Abstract Climate change poses a serious challenge to Indonesia^s livestock sector, especially beef cattle farmers in East Java who face productivity risks from changing rainfall patterns, drought, and rising temperatures. Adaptation is essential to ensure the sustainability of livestock enterprises and household economic resilience. This study analyzes factors influencing the choice of climate change adaptation strategies among beef cattle farmers in East Java. A survey with multistage sampling selected 300 farmers owning at least three head of cattle who had adopted adaptation strategies. Data were collected through structured interviews and analyzed using a Binary Logistic Regression (Logit Model) to examine the impact of household socio-economic characteristics on choosing on-farm or off-farm strategies. Results show that 70.33% beef cattle farmers preferred off-farm adaptation strategies, diversifying their livelihoods beyond livestock production. Farming experience emerged as the most influential factor shaping adaptive behavior, while limited access to agricultural residues reduced the likelihood of maintaining on-farm activities. The findings highlight the central role of human and natural capital in determining farmers^ resilience and adaptation pathways to climate change. Keywords: climate change, adaptation strategies, beef cattle farmers, East Java, Binary Logistic Regression Share Link | Plain Format | Corresponding Author (Niche Permata Sari)

7	Animal Agribusiness and Related Subjects	ABS-87
Land Valuation, Policy Literacy, and Livelihoods in Peri-Urban Poultry Systems: Evidence from Indonesia Jaisy Aghniarahim Putritamara (a*), Tina Sri Purwanti (a), Daranrat Jaitiang (b) a) Lecturer of the Department of Socio-Economics, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia b) Lecturer of the Department of Agricultural Economy and Development, Faculty of Agriculture Chiang Mai University Abstract Rapid urban encroachment is compressing Indonesia^s peri-urban farmland, raising uncertainty over siting, buffers, and rights an urgent threat to small and medium poultry producers and affordable protein access. We test whether producers perceptions of land valuation and their policy literacy jointly explain livelihood improvement in a peri-urban corridor of Malang Regency (East Java). A cross-sectional survey of 207 broiler producers was analyzed using PLS-SEM (SmartPLS) with three reflective constructs: land valuation, policy literacy, and livelihood improvement. The model explains 64.0% of variance in policy literacy and 53.3% in livelihood improvement. Land valuation is strongly and positively associated with policy literacy (&#946-=0.800, p<0.001) and significantly associated with livelihood improvement (&#946- = 0.302, p = 0.019). The policy literacy-livelihood path is positive but not statistically significant (&#946- = 0.242, p = 0.065), yielding a non-significant indirect effect (&#946- = 0.193, p = 0.067). Findings indicate that credible land signals value, transferability, and rights clarity primarily drive livelihood gains under peri-urban pressure, while policy literacy is presently complementary. Practical implications-prioritize transparent, locally legible land-valuation and rights services, and pair policy-literacy training with resource-enabling instruments (eco-financed biosecurity, streamlined permits) to convert knowledge into action and stabilize protein supply. Keywords: peri-urban agriculture- land valuation- policy literacy- livelihoods- poultry systems- PLS-SEM- Indonesia- urbanization- protein access Share Link | Plain Format | Corresponding Author (Jaisy Aghniarahim Putritamara)

8	Animal Agribusiness and Related Subjects	ABS-92
DEVELOPMENT STRATEGY OF THE PELUNG CHICKEN AGRIBUSINESS SYSTEM IN MALANG RAYA Ahmad Ridwan, Nevy Zaphira Putri Luni, Siti Azizah Faculty of Animal Science, Universitas Brawijaya Abstract This study aims to formulate strategies for developing the Pelung chicken agribusiness system in Malang Raya as an effort to strengthen the sustainability of local poultry farming and preserve Indonesia^s genetic resources. Pelung chicken (Gallus gallus domesticus) is a local breed with high economic and cultural value, particularly due to its distinctive crowing characteristics. The study utilized both primary and secondary data obtained through interviews, observations, and literature reviews. Data analysis was conducted using the Internal Factor Evaluation (IFE), External Factor Evaluation (EFE), SWOT Analysis, and Quantitative Strategic Planning Matrix (QSPM) to identify internal and external factors and to determine priority development strategies. The results showed that the total IFE score of 2.64 and EFE score of 2.87 placed the Pelung chicken agribusiness system in the ^hold and maintain^ position, indicating that the system is relatively stable and can be sustained and improved. The priority strategies derived from the QSPM analysis include: (1) optimizing local breeding stock with the support of academics and digital technology to produce superior breeds (TAS = 3.26), (2) utilizing experienced human resources and production technology to maintain breeding quality and prevent fraud (TAS = 3.13), and (3) developing pricing strategies based on niche market trends such as collectors, contests, and hobbyists (TAS = 3.07). Implementing these strategies highlights the importance of collaboration among farmer communities, academics, hobbyist associations, and the government in building an adaptive, competitive, and sustainable Pelung chicken agribusiness system. Keywords: pelung chicken, agribusiness, SWOT, QSPM, development strategy, Malang Raya. Share Link | Plain Format | Corresponding Author (Ahmad Ridwan)

9	Animal Agribusiness and Related Subjects	ABS-98
The Influence of Environmental Sustainability on Smallholder Farm Income Through Good Dairy Farming Practices at Malang Raya Indonesia Hari Dwi Utami, Lilik Eka Radiyati, Jaisy Aghniarahim Putritamara, Basya Hayun Nadyawati, and Prima Setyo Wiyono Faculty of Animal Sciences, Universitas Brawijaya, Malang, Indonesia Abstract Good Dairy Farming Practices (GDFP) have addressed to ensure the production of safe and high-quality milk from healthy animals through practices that are sustainable in terms of animal welfare, social, economic, and environmental aspects. Research was executed in Kemiri Village, Jabung Sub-district, Malang Regency, with the objective of analysing the implementation of GDFP from an environmental perspective and its relation to the economic return of smallholder dairy farming. The 80 respondents were selected by multistage sampling with purposive sampling method and they were members of the ^KUBE PSP Maju Mapan group^. The respondents were grouped into two scales: scale I (having 1- 6.37 Animal unit) and scale II (having 6.38 - 13.75 animal unit). Data were analysed by descriptive and multiple regression techniques. Results have found that first, dairy farmers were generally of productive age, predominantly male, with elementary education and 10-18 years of farming experience forage planting. Second, the farm scale-1, the use of forage and water efficiently, applying reduce, reuse and recycle farm waste as well as ensure appropriateness in overall operational dairy farming together has generated the farm income. The farm scale-1I, proper operational management enhanced farming income, although waste recycling practices were found to decrease farm returns. It is recommended that smallholder farmers improve the reuse, reduce and recycle (3R) of by-products from dairy farming operations to enhance both environmental sustainability and economic benefits. Keywords: reuse, reduce, recycle, waste, by-product Share Link | Plain Format | Corresponding Author (Hari Dwi Utami)

10	Animal Nutrition	ABS-1
The effectiveness addition various levels of essential amino acids into two types of rations to improve feed efficiency and performance of Alabio ducks aged 9 to 18 weeks Maijon Purba, Tiurma Pasaribu, Arnold P. Sinurat, Tatan Kostaman, Rudy Prasetyo, Jonathan Lase and Endang Sutedy National Research and Innovation Agency Bogor-Cibinong-Indonesia Abstract Determination nutritional requirements of rations, especially essential amino acids (lysine, methionine, threonine and tryptophan) (EAA) followed by the appropriate form of feed for duck farming is still relevant to be studied. An experiment was conducted to investigate the effect of addition different levels of EAA (0, 10, 20%) into two types of rations (mash, pelleted) to improve feed efficiency and performance of Alabio ducks. A total of 360 Alabio female ducks were kept and investigated since 9 to 18 weeks old (10 weeks of maintenance). All ducks were randomly kept in 36 pens with acrylic floors, length and width of pens were 3x2 m2 respectively. The ducks allocated into 6 treatments, 6 replications (10 ducks per replicate). The research was designed as a completely randomized design (CRD) in a 3x2 factorial pattern. The first factor were 3 levels of EAA (0, 10 and 20%), the second factor were 2 types of feed (mash and pellets). The variables observed included: feed consumption (FC), body weight gain (BWG), feed conversion ratio (FCR) and live body weight (LBW) were recorded weekly. The results showed that addition various levels of EAA into two types of rations had significant effect (P<0.05) on duck performance. FC of Alabio ducks with the addition of EAA into 2 types of rations (mash and pellet) had significantly different (P>0.05) compared to the control treatment. Supplementation various levels of EAA in 2 types of treatment rations for 10 weeks did not significant effect on the BWG and LBW of ducks, but had significant effect on the FCR variable (P<0.05). The best treatment ration to produce FC and FCR of Alabio ducks during 10 weeks of observation was achieved by the provision of T5 treatment ration (AAE level was 10% and feed given was pellet type). It was concluded that the addition 10% of AAE into pelleted type of rations at 9 to 18 weeks old increased feed efficiency and improved the performance of Alabio ducks. Keywords: Alabio duck, essential amino acids, types of ration, feed efficiency, performance Share Link | Plain Format | Corresponding Author (Maijon Purba)

11	Animal Nutrition	ABS-2
The Occurrence of Escherichia coli in Smoked Ready-to-eat (RTE) Donkey Meats Consumed in Ghana Martin Aduah1,*, Adamu Yusuf Kabiru2, Abubakar Asmau Niwoye2, and Frederick Adzitey3 (1) Africa Centre of Excellence for Mycotoxin and Food Safety, Federal University of Technology, Minna, Niger State, Nigeria. (2) Department of Biochemistry, Federal University of Technology, Minna, Niger State, Nigeria. (3) Department of Animal Science, University for Development Studies, Tamale, Ghana. * aduah.ac2117284[at]st.futminna.edu.ng Abstract Globally, foodborne infections- particularly antimicrobial resistant pathogens including bacteria are derailing efforts to achieve the sustainable development goals (SDGs) on health. This study was conducted to assess the occurrence of Escherichia coli (E. coli) in smoked ready-to-eat (RTE) donkey meats consumed in Ghana. A total of one hundred and fifty (150) swab samples were randomly collected from smoked donkey meats at various vending shops in the Doba and the Bolgatanga areas located in the Upper East Region of Ghana. The samples were examined for the occurrence of E. coli following the procedure described in the USA-FDA Bacteriological Analytical Manual. Antibiotic susceptibility test was also carried out on the isolates using the Kirby-Bauer disc diffusion method, and the results interpreted based on the clinical and laboratory standards institute (CLSI) guidelines. All data were analyzed using SPSS (version 20), and P<0.05 was considered significant. The results of the study showed that the occurrence of E. coli in the smoked RTE meat samples was 16.67% (25/150), whiles 125 (83.33%) samples tested negative. In addition, 60% of the isolates exhibited multiple, and different antibiotic resistance profiles, with the highest being TecCnAzmCroSxtCip (6 antibiotics). Physical examination of the vendors^ environment revealed that the vendors did not observe and adhere to strict hygienic and microbiological safety practices. The study revealed that some smoked donkey meats consumed in Ghana are contaminated with antibiotic resistant E. coli, an indication of poor post handling of the meat after smoking. This calls for enhanced surveillance and sensitization actions by stakeholders to avoid public health crisis in the study area and Ghana at large. Keywords: Keywords: Escherichia coli, Smoked donkey meat, SDGs, and Microbiological safety Share Link | Plain Format | Corresponding Author (Martin Aduah)

12	Animal Nutrition	ABS-6
Influence of Variety and Plant Fractions on Oxalate Content and Nutritional Composition of Pennisetum purpureum, Indigofera tinctoria, and Trichanthera gigantea Siti Nur Haslina Mamat1, Mohammad Mijanur Rahman2*, Renny Fatmyah Utamy3, Md Safiul Alam Bhuiyan2, Rovina Kobun2, Yuli Frita Nuningtyas4, Candyrine Su Chui Len2, Rohaida Abdul Rasid2 1) Faculty of Agro based Industry, Universiti Malaysia Kelantan, Jeli 17600, Kelantan, Malaysia 2) Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan 90509, Sabah, Malaysia *mijanur.r[at]ums.edu.my 3) Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan KM. 10, Makassar 90245, South Sulawesi, Indonesia. 4) Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Kota Malang, Indonesia Abstract Napier grass (Pennisetum purpureum) is one of the most important tropical forages due to its high biomass yield and favourable carbohydrate that supports efficient rumen fermentation. However, its utilisation is limited by the presence of anti-nutritional factors, particularly oxalic acid, which occurs at higher levels in Napier grass compared to other tropical forages. Excessive oxalate intake can disrupt calcium and magnesium metabolism, leading to metabolic disorders and kidney dysfunction in animals. This study aimed to determine the effects of variety and botanical fractions on oxalate content and proximate composition in Napier grass and selected non-Napier forages. Seven Napier grass varieties (Dwarf, Taiwan, Zanzibar, King, Purple, Pakchong, and Indian) as well as Indigofera tinctoria and Trichanthera gigantea were collected from farms in Kedah and Kelantan, Malaysia. For each plant type, leaves and stems were analysed separately for soluble oxalate, total oxalate, and proximate components contents. Among Napier grass varieties, Dwarf Napier grass exhibited the highest crude protein content, but also contained relatively high levels of soluble oxalate, particularly in the leaves. Overall, oxalate contents were consistently higher in leaves than in stems, mirroring the distribution of nutrients such as crude protein. These findings highlight the importance of considering both varietal selection and plant fractions when formulating feeding strategies to optimise nutritional value while minimising oxalate-related risks in ruminant diets. Selecting appropriate varieties and balancing leaf-to-stem ratios in rations could help reduce oxalate intake without compromising feed quality. Keywords: Botanical fractions, Napier grass, nutrient content, oxalate content, variety. Share Link | Plain Format | Corresponding Author (Mohammad Mijanur Rahman)

13	Animal Nutrition	ABS-7
Efficacy extract and hydrolysate protein from brown cricket (Gryllus assimilis) as an antibiotic for improving performance of quails Anna Lidiyawati (1,2), Suyadi suyadi (1), Eko Widodo(1), Muhammad Halim Natsir (1) (1)Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia (2)Department Animal science, University Nahdlatul Ulama Blitar, Blitar 66136, Indonesia Abstract Insects exctrac and hydrolisate are known to contain many bioactive peptides with antibacterial, antiparasitic, and antiprotozoal properties. The purpose of this study was to determine the influence of extract and hydrolysate protein from brown cricket (Gryllus assimilis) on the production performance of quails as antibiotic. A total of 400 female quails aged 6 weeks were reared and fed, watered and vaccinated according to recommendations in colony cages for 30 days. They were separated into four treatments with five replications, each with twenty quails. P0 = basal feed, P1 = basal feed with antibiotic 5 mg/kg, P2 = basal feed with insect extract 6 mg/kg, P3 = basal feed with insect hydrolysate protein 6 mg/kg. The administration of exctract and hydrolysate protein was carried out through feed. The variables measured were productivity (feed consumption, egg weight, feed conversion, cholesterol) quality of quail eggs (egg shell weight and thickness) and body resistance (white blood cell). The result show that the total cholesterol of egg yolks of P3 treatment was lower than the control. Administration of hydrolysate protein in P3 treatment increased the leukocyte value of quail compared to the control treatment . Administration of hydrolysate protein in P3 treatment increased the leukocyte value of quail compared to the control treatment (6.00 from 4.00, respectively). Although it did not have a significant influence on feed consumption, egg weight and feed conversion. In conclusion, applying hydrolysate protein of insect in feed can be utilized as a feed additive and as an alternative to antibiotics. Keywords: antibiotic, extrac , hydrolysate protein, brown cricket, quails Share Link | Plain Format | Corresponding Author (Anna Lidiyawati)

14	Animal Nutrition	ABS-11
Assessing the Effects of Lactobacillus spp. Isolated from Ensiled Swamp Forage on Body Weight, Carcass Characteristics, and Abdominal Fat in Local Ducks Fitra Yosi (a*), Sofia Sandi (a), Nuni Gofar (b), Eli Sahara (a), Meisji Liana Sari (a), Rizki Palupi (a), Farin Farandhita (a) a) Department of Animal Science, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30662, Indonesia *fitrayosi[at]unsri.ac.id b) Department of Soil Science, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30662, Indonesia Abstract Using lactic acid bacteria, such as Lactobacillus, as probiotics in poultry farm is a common practice. However, little research has been conducted on the application of Lactobacillus derived from swamp forage silage. This study aims to investigate the supplementation of Lactobacillus spp. derived from swamp grass (Hymenachne acutigluma) silage on body weight, carcass characteristics, and abdominal fat in local ducks. A total of sixty 24-week-old ducks were randomly allocated into five treatment groups with four replicates per group using a completely randomized design and were reared for 60 days. The treatment groups included a control group without Lactobacillus (LAC) supplementation and groups LAC1, LAC2, LAC3, and LAC4, which were supplemented with a Lactobacillus solution in their drinking water at concentrations of 1x106, 107, 108, and 109 CFU/mL, respectively. At 33 weeks of age, the ducks final weight was measured. Then, one duck per replicate was slaughtered to analyze carcass characteristics and abdominal fat percentage. The data were analyzed using analysis of variance, followed by a Duncan multiple range test. The results showed that administration of the LAC solution increased carcass and thigh slice percentages (p < 0.05). The LAC3 group had a higher carcass percentage than the control group and the other LAC groups (p < 0.05). Adding the LAC solution also reduced the percentage of abdominal fat (p < 0.05). However, the LAC solution did not affect final body weight. In conclusion, supplementing drinking water with an LAC solution at concentrations up to 1x 109 CFU/mL improved carcass yield, particularly in thigh cuts, and decreased abdominal fat production in ducks. Keywords: Abdominal fat, Body weight, Carcass yield, Ducks, Lactobacillus, Swamp forage silage Share Link | Plain Format | Corresponding Author (Fitra Yosi)

15	Animal Nutrition	ABS-16
Kandis Acid Extract (Garcinia xanthochymus) as Acidifier in Drinking Water: The Impact on Performance, Digestibility of Dry and Organic Matter, and Total Microbes in Native Chickens Sofia Sandi, Rizki Palupi, Muhakka, Riswandi, Eli Sahara, Meisji Liana Sari, Asep Indra Munawar Ali, Fitra Yosi, and Anggriawan Naidilah Tetra Pratama* Department of Animal Science Technology and Industry, Faculty of Agriculture, Sriwijaya University, South Sumatra, Ogan Ilir, Indonesia 30662 Tel (0711)-581106 *anggriawan[at]fp.unsri.ac.id Abstract Kandis acid was obtained herbally and can be used as an acidifier. Kandis acid contains organic acids in the form of hydroxycitric acid which allows for an increase in the quality of digestibility of poultry production. The study was aimed to determine the effect of giving kandis acid extract in drinking water on performance, digestibility, and total microbes of native chickens. The research was conducted in August - October 2023, in the Laboratory and Poultry Cage of the Animal Husbandry Study Program, Department of Animal Husbandry Technology and Industry, Faculty of Agriculture, Sriwijaya University. The research design used was a completely randomized design (CRD) consisting of 4 treatments and 6 replicates, and each experimental cage unit consisted of 2 native chickens. The treatments used were P0 (control treatment without acidifier), P1 (kandis acid solution with pH 2.4), P2 (kandis acid solution with pH 3.4) and P3 (kandis acid solution with pH 4.4). The observed variables were performance, digestibility of dry matter (DMD), digestibility of organic matter (DOM) and total microbes. Data were analyzed by ANOVA and if it showed a significant difference, it was continued with the Duncan Multiple Range test. The provision of kandis acid extract in drinking water had a significant effect (P<0.05) on the total number of E-coli bacteria in the cecum of 0.92-3.85 log cfu/g, dry matter digestibility of 73.39-75.02% and organic matter digestibility of 73.63-75.12%, but had no significant effect on performance, total microbes, and total Lactic acid bacteria (LAB). Giving kandis acid extract with pH 3.4 in drinking water gives optimal results on dry matter digestibility, organic matter digestibility, and total E-colli bacteria in native chickens. Keywords: Acidifier, Asam kandis extract, Digestibility, Kampung Chickens, Performance. Share Link | Plain Format | Corresponding Author (anggriawan pratama)

16	Animal Nutrition	ABS-17
Drying methods comparison of carrot (Daucus carota) using vacuum and oven based on l*a*b color and total carotene content Aliefianda Ilhamdani Alzi Pratama1, Muhammad Halim Natsir2, Osfar Sjofjan2, Feri Eko Hermanto2, Yuli Frita Nuningtyas2* 1 Student of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2 Lecturer of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia fritanuningtyas[at]ub.ac.id Abstract This study was conducted to compare the effects of different drying methods on the Lab* color of carrot. The research method was using a descriptive comparative approach. Carrot was obtained from the market and cleaned with running water and cut into thin slices approximately 2 mm and blanched using 90 deg C water for 3 minutes then transferred into an ice-cold water to stop the cooking method. Carrot then divided into two groups each for their corresponding drying method, the first group was vacuum dryer with 60 deg C and the second was oven with 60 deg C, both methods were used until the sliced carrot became crisp and can be grinded into powder. Carrot powder then examined using CIELAB to determine the value of each color and sent to the Faculty of Agricultural Technology to be analyzed for the carotene content. Data obtained were then analyzed using t-test to determine the significance of drying methods on each color value. The results showed a significant difference in all color components between the drying methods. Oven drying produced carrots with higher L* values (mean = 63.43) compared to vacuum drying (mean = 60.73), indicating greater lightness retention (p < 0.0001) while vacuum drying produced carrots with higher a* values (mean = 19.05) and b* (mean = 23.60) values compared to oven drying (a* mean = 14.87- b* mean = 22.83), indicating enhanced red and yellow tones. The differences were highly significant for L* and a* (p < 0.0001) and significant for b* (p < 0.01). Vacuum dried carrot has higher total carotene (767.84 ug/g) than oven (703.83 ug/g). These finding suggests that oven drying better preserves lightness while vacuum drying better preserves the visual quality of carrots, making it a preferable method to maintain color and total carotene content post-harvest. Keywords: Carrot, drying method, color, carotene, preservation, vacuum drying. Share Link | Plain Format | Corresponding Author (Aliefianda Ilhamdani Alzi Pratama)

17	Animal Nutrition	ABS-19
Valorization of Brown Seaweed (Sargassum aquifolium) Alginate for Potential Sustainable Animal Feed Applications Felicia Ling Wen Xia1, Rovina Kobun2 1Food Security Research Lab, Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia 2Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Locked Bag No. 3, 90509, Sandakan, Sabah, Malaysia Abstract The search for sustainable feed additives is vital to balance livestock productivity, welfare, and environmental stewardship in line with the Sustainable Development Goals (SDGs). Brown seaweed (Sargassum aquifolium), an abundant yet underutilized marine biomass, was investigated as a source of sodium alginate for animal feed applications. Alginate was extracted through ultrasound-assisted extraction yielding 51.63% (dry weight basis). Structural characterization via FTIR confirmed the presence of glycosidic linkages between &#946--D-mannuronic acid (M) and &#945--L-guluronic acid (G) residues. Rheological testing revealed excellent gel-forming and thickening capacities, supporting its potential role as a natural binder in pelleted feeds. Functional assays demonstrated moderate antioxidant potential, with DPPH radical scavenging activity (IC&#8325-&#8320-: 0.65 mg/mL). Moreover, alginate exhibited high water-holding and oil-binding capacities, which may improve nutrient digestibility and feed stability. From an environmental perspective, utilizing Sargassum biomass helps mitigate the ecological burden of seaweed overaccumulation while advancing circular bioeconomy strategies. These findings highlight S. aquifolium alginate as a multifunctional, potential eco-friendly feed ingredient capable of reducing reliance on synthetic additives, enhancing animal welfare, and promoting sustainable productivity. Keywords: Sargassum aquifolium, alginate, animal feed, sustainability, livestock Share Link | Plain Format | Corresponding Author (Felicia Ling Wen Xia)

18	Animal Nutrition	ABS-22
Study of hematological parameters of laying hens at starter phase using Gropro as natural feed additive Sarmad M , Sjofjan O, Natsir MH, Widodo E. Djunaidi IH, Nuningtyas, Y, and Fayuan G. 1Student of Phd. Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia. sarmad23[at]student.ub.ac.id 2Professor at Faculty of Animal Science, Universitas Brawijaya, Malang 65145, osfar[at]ub.ac.id 3Professor at Faculty of Animal Science, Universitas Brawijaya, Malang 65145, emhanatsir[at]ub.ac.id 4Professor at Faculty of Animal Science, Universitas Brawijaya, Malang 65145, eko.widodo[at]ub.ac.id 5Professor at Faculty of Animal Science, Universitas Brawijaya, Malang 65145, irjuna[at]ub.ac.id 6Lecturer at the Faculty of Animal Science, Universitas Brawijaya, Malang, fritanuningtyas[at]ub.ac.id 7Angel Yeast Pte.Ltd., 25A Jubilee Rd, Singapore 128567 Abstract Hematological parameters were evaluated by conducting trial investigations on Day old chicks Hy-Line strain using GroPro as a feed additive under open house management systems at field laboratory of Sumber Sekar, Malang Easy Java. Fresh water and feed were offered ad libitum. The total number of chicks 480 which were divided into four treatment groups with six replications each, using a Completely Randomized Design. The treatments included P0 (control, no GroPro), P1 (0.2% GroPro), P2 (0.5% GroPro), and P3 (1% GroPro) with duration of 7 weeks. In total 8 blood samples were drawn in random be through brachial cut at the close of trials. The data were analysed by using ANOVA analysis, and if the result is significant then continued with the Duncan^s Multiple Range Test. The results show the treatments were significantly (P<0.01) affected all variables including Hemoglobin (Hb), Mean Corpuscular Hemoglobin (MCH), Packed cell volume (PCV), Red Blood Cell (RBC), White Blood Cell (WBC), except Mean Corpuscular Hemoglobin Concentration (MCHC) which shows no significant result (P>0.05). Therefore, it is concluded that GroPro at level 1% inclusion positively influences hematological study of laying hens at starter phase well define its health status and help-out in diagnosis and treatment of birds. Keywords: Natural feed additives, Hematological parameter, Laying hens, Starter phase Share Link | Plain Format | Corresponding Author (Muhammad Sarmad)

19	Animal Nutrition	ABS-23
The Evaluation Effect Of Onion Peel Extract (Allium cepa L.) on Scanning Electron Microscopy (SEM), Density and Inhibity Microbe as Poultry Additive Della Ariesba Putri Dulia 1), Osfar Sjofjan 2*), Edhy Sudjarwo 2) 1) Student of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2) Lecturer of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract This study aims to evaluate the scanning electron microscopy (SEM), density, and bacterial inhibition of onion peel as a potential poultry feed additive. The material used in this study was onion peel. Particle morphology was analyzed using a Scanning Electron Microscope (SEM) DEX tool with 10,000x magnification to observe particle structure and identify chemical elements. The density test was conducted to determine the volume occupied by particle mass. The bacterial inhibition test was carried out to identify the antimicrobial activity against E. coli, Salmonella sp. and lactic acid bacteria (LAB) around the wells. The results showed that the shape of the onion peel particles was irregular, resembling logs and lumps. The density of onion peel extract was (225 g/L). The inhibition test indicated that the solid extract of onion peel exhibited stronger antimicrobial activity compared to the solution or liquid extract. The inhibition zones of the solid extract was (10 mm) E. coli, (11.2 mm) Salmonella sp. and (14.2 mm) LAB. It can be concluded that onion peel extract have potential as a feed additive due to its digestible particle morphology (SEM), high particle density, and strong antimicrobial activity. Keywords: Additive, Density, Extraction, Microbial Inhibity, Onion Peel, SEM Share Link | Plain Format | Corresponding Author (Della Ariesba Putri Dulia)

20	Animal Nutrition	ABS-24
Evaluation of Particle Size, Total Flavonoid, and Antioxidant Activity of Onion Peel (Allium cepa L.) in Different Forms as a Poultry Feed Additive Miftahul Jannah 1), Osfar Sjofjan 2*), Irfan H.Djunaidi 2) 1) Student of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2) Lecturer of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract This study aims to evaluate the particle size, flavonoid content, and antioxidant activity of onion peel in various forms as a poultry feed additive. The material used in this study was onion peel. Particle size was analyzed using laser diffraction to obtain uniform particle size data. Flavonoid content was determined using UV-Vis spectrophotometry to obtain the total flavonoid content in onion peel. Antioxidant activity was tested using the DPPH method with a UV-Vis spectrophotometer to determine the ability of onion peel to scavenge free radicals. The results showed that the particle size of onion peel in powder form was (36.24 micrometer), solution form (0.98 micrometer), and extract form (3.47 micrometer). The highest flavonoid content was found in the powder form (335.97 mg QE/g), followed by the extract form (11.91 mg QE/g), and the solution form (1.66 mg QE/g). The strongest antioxidant activity was demonstrated by the IC50 value of the extract (1.69 microgram/mL), followed by the powder (8.57 microgram/mL), and the solution (458.18 microgram/mL). It can be concluded that onion peel have the potential to be used as a feed additive, as indicated by its particle size, flavonoid content, and antioxidant activity. Keywords: Antioxidant Activity, Flavonoid Content, Onion Peel, Particle Size, Poultry Feed Additive. Share Link | Plain Format | Corresponding Author (Miftahul Jannah)

21	Animal Nutrition	ABS-26
Influence of Various Sugar Sources as Drinking Water Additives on the Hematological Profile of Broiler Chickens (Gallus gallus domesticus L.) Angeline M. Villacin (1) and Richelle A. Niepes (1) College of Environment and Life Sciences, Mindanao State University at Naawan Abstract This study assesses the impact of various sugar sources (dextrose, honey, molasses, and brown sugar at 50g/L) as water additives on broiler chickens, focusing on blood profiles. The experiment, employing a completely randomized design, involved varying inclusion levels of these sugar sources in drinking water. A total of 48 broiler chickens were distributed into treatments, with four replicates each. All four treatment: T0 (control) with 50g/L dextrose added to water, T1 with 50g/L honey added to water, T2 with 50g/L molasses added to water and T3 with 50g/L brown sugar added to water were administered. The analysis included blood weight, protein levels, hematology, and red cell indices to determine how sugar additives affect health indicators. Results indicated that blood weight and the percentage of blood weight per live weight basis were not varied significantly (p > 0.05) among treatments. Blood protein levels, including total protein, albumin, and globulin, showed no significant (p > 0.05) differences among treatments, remaining within normal ranges, suggesting that sugar supplements did not adversely affect protein metabolism or immune function. Hematology analysis revealed that hematocrit and hemoglobin values were outside normal ranges, but no significant (p > 0.05) treatment effects were observed. RBC and WBC counts were within normal ranges, indicating no appreciable effects from sugar inclusion. Regarding red cell indices, MCV values showed statistical differences (p < 0.05), but remained within normal ranges. MCH and MCHC values also varied, with no significant (p > 0.05) differences among treatments. The study concludes that sugar sources as water additives can influence blood weight and red cell indices without negatively impacting overall blood health. These findings suggest potential benefits for poultry production through optimized sugar-based additives. Keywords: Natural sugars, blood weight, blood protein levels, hematology, red cell indices. Share Link | Plain Format | Corresponding Author (Richelle Niepes)

22	Animal Nutrition	ABS-34
Fermentation of Cassava Leaves Using Microbial-Nutrient Additives: Impact on HCN and Fiber Content Yusni Khairani Tampubolon (a*), Fuad Hasan (a), Winda Fransisca Saragih (a), Muhammad Amran (a) a) Departement of Animal Science, Agriculture Faculty, Universitas Sumatera Utara, Medan 20155, Indonesia *yusnikhairani[at]usu.ac.id Abstract This study aims to evaluate the effectiveness of cassava leaf (Manihot esculenta sp.) fermentation using a combination of microbial (commercial EM4 and local microorganisms from fruit, MOL) and a nutrient additive (commercial Viterna) on the content of hydrocyanic acid (HCN), neutral detergent fiber (NDF), acid detergent fiber (ADF), and pH. The study used four treatments and five replications, namely: T0 (Fresh cassava leaf without addtives), T1 (fermentation with 40% EM4, 20% ml Viterna, and 40% ml MOL), T2 (20% EM4, 40% Viterna, and 40% MOL), and T3 (40% EM4, 40% Viterna, and 20% ml MOL). Each treatment was applied at a total rate of 0.5% (v/w) fresh cassava leaves, equivalent to 5 mL per kg of material. The samples were fermented anaerobically for 14 days, followed by analysis of HCN, NDF, ADF, and pH. Data were analyzed using analysis of variance based on a Completely Randomized Design and Duncan further test. The results showed significant differences (P<0.05) in HCN, NDF, ADF and pH levels among treatment. The content of HCN T2 (2.79+/-0.93 mg/kg) and T3 (3.43+/-0.56 mg/kg) was lower than T0 and T1. The lowest pH values were found in T2 (4.01+/-0.00) and T3 (4.02+/-0.02). The lowest NDF and ADF values were found in T3, with 42.55+/-0.23% and 32.08+/-0.13%, respectively. It can be concluded that cassava leaves fermentation using a combination of microbial-nutrient additives in T3, effectively reduced HCN content and improved fiber quality. Keywords: : Cassava leaves- fermentation- microbial-nutrien, Hydrogen Cyanide (HCN), Fiber Quality Share Link | Plain Format | Corresponding Author (Yusni Khairani Tampubolon)

23	Animal Nutrition	ABS-35
Phenotypic Characteristics of Lactic Acid Bacteria Isolates in Poultry Based on Total Plate Count, Gram Reaction, and Hemolytic Activity. Amara Brilliant Marcelline Alfariza1, Muhammad Halim Natsir2, Feri Eko Hermanto2, Yuli Frita Nuningtyas2* Universitas Brawijaya Abstract This study aims to characterize Lactic Acid Bacteria (LAB) isolates isolated from poultry based on key phenotypic traits, including microbial abundance through Total Plate Count (TPC), basic identity using Gram Reaction, and safety potential through Hemolytic Test. Bacterial samples were isolated from groups of free-range chickens and broilers, and TPC calculations were performed to determine the poultry group with the highest microbial population. The results of the microbial abundance analysis between the two poultry groups were analyzed using an independent t-test to determine statistical differences. Isolates from the poultry group with the best TPC values were then continued for further testing. The Gram Reaction Test was performed to confirm that the isolates were Gram-positive bacteria, which is a characteristic of the LAB group. Initial safety assessment was carried out through the Hemolytic Test by planting the isolates on blood agar media. The test results showed that the LAB isolates that were successfully isolated were Gram-positive, in accordance with the characteristics of this bacterial group. The results of the Hemolytic Test showed that lactic acid bacteria were included in the gamma hemolytic category which is an important criterion in the selection of safe probiotic candidates for poultry. Keywords: Lactic Acid Bacteria, Total Plate Count, Gram Reaction, Hemolytic Test Share Link | Plain Format | Corresponding Author (Amara Brilliant Marcelline Alfariza)

24	Animal Nutrition	ABS-36
Evaluation of Nutritional Content of Total Mixed Ration (TMR) Based on Rice Straw Fermented with Saccharomyces cerevisiaeAbstract Adyatma Rijal Rasyid1, Nuaf Praditya1, Desta Rahmania Syafitri1, Ramdhan Wardianto1, Nisrina Fathina Haura1, Asri Nurul Huda1 1Department of Animal Science, Faculty of Animal Husbandry, Universitas Brawijaya 65145 Abstract East Java is one of the largest contributors of agricultural land in Indonesia, accounting for 25% of the country^s total agricultural land. The area of rice cultivation land in East Java reaches 1.62 million hectares. The utilization of rice straw is still not optimal due to public concerns in processing rice straw, as it contains high levels of lignin, cellulose, and hemicellulose. The use of urea in fermented rice straw is still debated by the public due to fears that urea may poison livestock. Substituting urea with Saccharomyces cerevisiae as the fermentation agent for rice straw is considered capable of improving the nutritional quality of rice straw, and with the addition of concentrate, it can be formulated into Total Mixed Ration (TMR). The observation of nutritional quality was conducted at the Animal Nutrition Laboratory, Faculty of Animal Husbandry, Universitas Brawijaya, and the Sumber Sekar Field Laboratory, Faculty of Animal Husbandry, Universitas Brawijaya, from September 12 to November 1, 2025. The materials used in this study were rice straw, yeast (Saccharomyces cerevisiae), concentrate (SMG Mixfeed S18), pH buffer, distilled water, H2SO4 solution, NaOH solution, and Na2SO3 solution. The tools used in this research included plastic bags, a digital scale (0.01), scissors, adhesive tape, pH meter, thermometer, chopper, label paper, porcelain crucibles, desiccator, furnace, tongs, AI-disk, catalyst, dispenser, Erlenmeyer flask, measuring glass, and crucible. The complete feed rations were formulated into T1, T2, T3, and T4. All stages of the study were conducted at the nutrition laboratory, including problem analysis, physical quality observation, improvement of rice straw nutrition, and reduction of fiber content. Rice straw showed an increase in nutritional quality, especially in crude protein. The crude fiber of rice straw decreased, making it suitable for livestock feed. The highest crude protein content of complete feed based on fermented rice straw was found in T2, with 12.25% crude protein. The highest crude fiber content was also in T2, with 20.82%. Thus, the T2 complete feed was the best, producing the highest crude protein content and the lowest crude fiber content. Keywords: rice straw, Saccharomyces cerevisiae, concentrate, complete feed, nutritional content Share Link | Plain Format | Corresponding Author (adyatma rijal rasyid)

25	Animal Nutrition	ABS-47
The Effect of Solvent Ratio with Mahkota Dewa Fruit (Phaleria macrocarpa) Extraction Result and Antimicrobial Activity for Escherichia coli and Salmonella sp. Najma Afrahun Nufus 1), Eko Widodo 2), Heli Tistiana 2) 1) Student of Faculty of Animal Scince, Universitas Brawijaya, Malang, Indonesia 2) Lecturer of Faculty of Animal Science, Universitas Brawijaya Malang, Indonesia Abstract The research aimed to determine the effect of solvent ratio Mahkota Dewa fruit (Phaleria macrocarpa) on the extraction results as a feed additive to determine the antimicrobial activity of Escherichia coli and Salmonella Sp. The materials used are fresh Mahkota Dewa fruit (Phaleria macrocarpa) and aquadest. The extraction method used in this study is Ultrasound-Assisted Hydrodistillation Extraction (UAHDE). The antimicrobial activity in this study is the inhibition zone of Mahkota Dewa fruit extract against Lactic Acid Bacteria, Escherichia coli and Salmonella Sp using the agar well diffusion method. The results showed that with an extract ratio of 1: 2 produced a yield of (87.3 ml), 1: 4 (362 ml), and 1: 6 (486 ml). Comparison of the ratios of 1: 4 and 1: 6 showed bacterial inhibition, especially against salmonella, using a concentration of 100% with an inhibition zone of 1:4 (3.55 mm) and 1:6 (3.24 mm) and Lactic Acid Bacteria could not be killed. It can be concluded that the extract of the mahkota dewa fruit have the potential to be used as a feed additive. Keywords: Antimicrobial Activity, Solvent Ratio, Phaleria Macrocarpa, Poultry Feed Additive Share Link | Plain Format | Corresponding Author (Najma Afrahun Nufus)

26	Animal Nutrition	ABS-48
EVALUATION ON YEAST PROFILE ON FIBER QUALITY IN AGRICULTURAL WASTE-BASED FEED FERMENTATION Allya Ratna Ardilla, Achmad Nicko Setya Ingshagi, Rasyid Farda Hidayatulloh, and Asri Nurul Huda Brawijaya University Abstract Indonesia faces difficulties in providing grass feed for ruminant livestock. The low quality of wild grass cannot meet nutritional needs, resulting in low livestock productivity. Therefore, many farmers use agricultural waste such as corn stalks and rice straw as alternative feed ingredients. The low nutritional content of rice straw and corn stalks, along with their difficult digestibility, requires treatment to improve their nutritional value. One way to increase their nutritional content is through fermentation. Fermentation is one effort that can be done to improve the nutritional quality of feed through the activity of microorganisms such as yeast. This study aims to evaluate the effect of yeast profiles on fiber quality in fermented feed based on agricultural waste. The study will use a Completely Randomized Design with two treatments: P0 (without any addition) and P1 (with the addition of 5% molasses), each with four replications. The parameters observed include the content of Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose, hemicellulose, and lignin. The expected results of this research are increased yeast activity that can reduce crude fiber content and improve the quality of feed fiber, so that agricultural waste can be used as an alternative feed that is more efficient and has better nutritional value for livestock. Keywords: agricultural waste, fermentation, yeast, crude fiber Share Link | Plain Format | Corresponding Author (Allya Ratna Ardilla)

27	Animal Nutrition	ABS-49
Total Mixed Ration Composition Based on Rice Straw with Yeast Fermentation Technology (Saccharomyces cerevisiae): Effects on Physical Quality and Proximate Composition Nuaf Praditya, Adyatma Rijal Rasyid, Desta Rahmania Syafitri, Ramdhan Wardianto, Nisrina Fathina Haura and Asri Nurul Huda Department of Animal Science, Faculty of Animal Science, Brawijaya University 65145 sangpemimpi1400[at]student.ub.ac.id Abstract Rice straw has the potential to be used as a raw material for making alternative feed for livestock. The implementation of alternative feed is one solution to overcome the urgent problem of feed shortages for ruminants, especially cattle. Total Mixed Ration (TMR), commonly known as complete feed, is a type of mixed feed consisting of several feed ingredients such as concentrates, forage, and other feed ingredients, providing complete nutritional content. This study aims to evaluate the physical quality (aroma, color, texture, pH, temperature, and presence of fungi) and nutritional content (crude protein, dry matter, and organic matter) of fermented TMR using rice straw with different ratios of rice straw and concentrate. The study was conducted at the Sumber Sekar Field Laboratory and the Nutrition Laboratory of the Faculty of Animal Science, Brawijaya University. The study used a completely randomized design (CRD) with 4 treatments and 4 replicates, namely T1= rice straw 80% : concentrate 20% added 4% Saccharomyces cerevisiae, T2= rice straw 70% : concentrate 30% added 4% Saccharomyces cerevisiae, T3= rice straw 60% : concentrate 40% added 4% Saccharomyces cerevisiae, and T4= rice straw 50% : concentrate 50% added 4% Saccharomyces cerevisiae. The analysis results showed that the fermented total mixed ration based on rice straw had a significant effect (P<0.05) on physical quality (aroma, color, texture, pH, temperature, and presence of fungi). In nutritional content, the highest crude protein content is T2, with a crude protein content of 12.25%. The highest dry matter content is found in T2, with a dry matter content of 88.67%. The highest organic matter content is found in T2, with an organic matter content of 66.08%. Treatment T2 is the best total mixed ration based on rice straw, producing the highest crude protein, dry matter, and organic matter content. Keywords: Total Mixed Ration, Rice Straw, Fermentation, Saccharomyces cerevisiae, Physical Quality, Proximate Composition Share Link | Plain Format | Corresponding Author (Nuaf Praditya)

28	Animal Nutrition	ABS-50
Analysis of Physical and Nutrient Quality and Lactic Acid Producing Yeast Profile in Corn Stover Silage Achmad Nicko Setya Ingshagi(a), Rasyid Farda Hidayatulloh(b), Allya Ratna Ardilla(c), Asri Nurul Huda(d) Animal Science, Brawijaya University Jalan Veteran 10, Malang City, East Java 65145 Abstract Feed supply fluctuation remains a major constraint for ruminant production in tropical regions, where green forage availability decreases sharply during the dry season. Ensiling corn stover is a strategic approach to preserve feed while maintaining nutrient quality. This study aimed to analyze the physical quality, nutrient composition, and profile of lactic acid producing yeast in corn stover silage. The experiment was arranged in a Completely Randomized Design consisting of three treatments with four replications: P0 (without additive), P1 (with 5 percent molasses), and P2 (with ground corn). The evaluated parameters included pH, color, aroma, texture, dry matter, organic matter, crude protein, and yeast characteristics. The research was conducted at the Faculty of Animal Science, Universitas Brawijaya. The expected results indicate that the addition of molasses and ground corn enhances fermentation quality by lowering pH, improving nutrient stability, and supporting beneficial yeast activity that contributes to lactic acid formation. This study provides new insights into the role of lactic acid producing yeast in corn stover silage and supports sustainable feed preservation for ruminant livestock in tropical environments. Keywords: Corn stover silage, physical quality, nutrient content, lactic acid yeast, fermentation Share Link | Plain Format | Corresponding Author (Achmad Nicko Setya Ingshagi)

29	Animal Nutrition	ABS-56
Analysis of Physical and Nutrient Quality and Lactic Acid Producing Yeast Profile in rice straw Rasyid Farda Hidayatulloh(a), Achmad Nicko Setya Ingshagi(b), Allya Ratna Ardilla(c), Asri Nurul Huda(d) Animal Science, Brawijaya University Jalan Veteran 10, Malang city, East Java 65145 Abstract Fluctuations in feed availability remain a major constraint in ruminant livestock production in tropical regions, especially during the dry season when fresh forage is difficult to obtain. The production of rice straw silage is an important strategy for preserving feed while maintaining its nutritional quality. This study aims to analyze the physical quality, nutritional content, and lactic acid-producing yeast profile of rice straw silage. The experiment was designed using a completely randomized design with three treatments and four replicates, namely P0 (no additives), P1 (5% molasses addition), and P2 (5% molasses and rice bran addition). The parameters observed included pH, color, aroma, texture, dry matter, nutrient content, and fermentation characteristics. The research was conducted at the Faculty of Animal Science, Brawijaya University. The expected results showed that the addition of molasses and rice bran was able to reduce pH, increase fermentation stability, and improve nutrient content and lactic acid formation in rice straw silage. This study provides new insights into improving the quality of rice straw silage and supports efforts to conserve sustainable feed for ruminants in tropical regions. Keywords: rice straw silage, physical quality, nutritional content, lactic acid bacteria, fermentation Share Link | Plain Format | Corresponding Author (Rasyid Farda Hidayatulloh)

30	Animal Nutrition	ABS-59
Management Practices and Subclinical Mastitis in Bandung Regency: A Comparative Study of Intensive and Semi-Intensive Farms Yusuf Subagyo 1, Hermawan Setyo Widodo 1, Merryafinola Ifani 1, Afduha Nurus Syamsi 1, Asep Rahmat Khaerudin 2, Arif Pujiyono 3 1. Faculty of Animal Science, Jenderal Soedirman University, Purwokerto 2. Faculty of Animal Science, Persatuan Islam University, Bandung 3. Farming High School Cikole, Bandung Abstract Mastitis is a primary disease affecting dairy farm productivity, and its prevalence is closely linked to herd management practices. In Bandung Regency, a major hub for dairy production, farms operate under diverse intensive and semi intensive systems. This study aimed to evaluate and compare the occurrence of subclinical mastitis relative to management practices in these two systems. A cross sectional study was conducted in the Lembang and Pangalengan areas, involving two intensive farms (n 25 cows) and nine semi intensive farms (n 32 cows). Subclinical mastitis was assessed using the California Mastitis Test (CMT), with scores averaged per animal. Farmer interviews were also conducted to determine the frequency of CMT screening. Data were analyzed using descriptive statistics. The results indicated that intensive farms performed CMT more frequently (2.48 times per lactation) and more recently (2.68 months ago) than semi intensive farms (1.16 times per lactation and 17.63 months ago, respectively). Consequently, the mean CMT score, indicating mastitis severity, was significantly lower in intensive farms (0.84) compared to semi intensive farms (1.94). In conclusion, the higher prevalence of subclinical mastitis in semi intensive farms is strongly linked to infrequent screening. This was attributed by farmers to significant barriers, including the low availability, inconsistent supply, and high cost of CMT reagent. These findings highlight that improving farmer education on udder health must be paired with policies to increase the accessibility and affordability of diagnostic tools to enhance milk quality and farm profitability. Keywords: Subclinical Mastitis, California Mastitis Test (CMT), Dairy Management, Supply Chain, Smallholder Farmers, Bandung Share Link | Plain Format | Corresponding Author (Hermawan Setyo Widodo)

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