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Animal Reproduction and Breeding |
ABS-84 |
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Evaluation of the Success of Artificial Insemination Using Sexed Semen Based on the Same Bull by Different Inseminators in Friesian Holstein Dairy Cows
Muhammad Fathir Zaidaan Widiyanto (a), Habib Asshidiq Syah (b), Aulia Puspita Anugra Yekti (b), Trinil Susilawati (b)
a) Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
Abstract
The purpose of this study was to evaluate the success of artificial insemination using sexed semen based on different inseminators using the same bull in Friesian-Holstein cows. This study was conducted from January to October 2025. The subjects of this study were five inseminators from KAN Jabung and 649 Friesian-Holstein cows that were artificially inseminated using sexed semen from Glens bulls. Observations made on the inseminators included their skills in AI, such as: accuracy in insemination timing, semen selection, and semen thawing. The used bull semen was from Glens FH bulls. The research method used was quantitative descriptive. The parameters observed included Conception Rate (CR), Pregnancy Rate (PR), Service per Conception (S/C), and Inseminator Success. The data obtained were then analyzed with descriptive analytics method. The results showed that inseminator 1 produced the highest CR value with a percentage of 17.88%, while inseminator 4 produced the lowest CR with a value of 11.49%. The average CR value of the 5 inseminators was 14.16%. The PR value was highest for inseminator 1 with a percentage of 27.37%, while the lowest percentage was produced by inseminator 4 with a percentage of 12.64%. The average PR percentage of the 5 inseminators was 17.87%. The highest S/C value was produced by inseminator 2 with an S/C value of 1.05, while the lowest S/C value was produced by the inseminator 1 with an S/C value of 1.37. The average S/C value of the 5 inseminators was 1.19. The highest inseminator success percentage was obtained by inseminator 1 with a value of 21.12%, while the lowest value was obtained by inseminator 4 with a value of 11.22%. The average inseminator success percentage was 15.12%. It can be concluded that the success of artificial insemination using sexed semen is influenced by the quality of the semen, physiological conditions of dairy cows, the accuracy of semen deposition, abilities, and inseminator skills in performing AI.
Keywords: Artificial Insemination, Friesian Holstein, Sexed Semen, Inseminator
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| Corresponding Author (Muhammad Fathir Zaidaan Widiyanto)
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| 92 |
Animal Reproduction and Breeding |
ABS-85 |
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EVALUATION OF THE SUCCESS OF ARTIFICIAL INSEMINATION USING FROZEN SEMEN BASED ON THE SAME BULL WITH DIFFERENT INSEMINATORS
Wahyu Yudha Setiawan (a), Habib Asshidiq Syah (a), Aulia Puspita Anugra Yekti (a), Trinil Susilawati (a)
a) Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
Abstract
The purpose of this study was to evaluate the success of AI using frozen semen from the same bull with different inseminators. This study was conducted from January 2025 to October 2025. The subjects of this study were 6 inseminators from the Jabung Agro Niaga Cooperative and 147 cows. Observations of the inseminators included their skills in AI, such as: timeliness of insemination, semen selection, and semen thawing. The bull semen used was from FH Mountsi bulls. The research method used was quantitative descriptive. The parameters observed included the Conception Rate (CR), Pregnancy Rate (PR), Service per Conception (S/C), and Inseminator Success for each inseminator. The data was analyzed using Microsoft Excel and presented in tables, graphs, and descriptions. The results showed that inseminator 1 produced the highest CR with a percentage of 26.92%, while inseminator 4 produced the lowest CR percentage of 9.54%. The average CR percentage of the six inseminators was 18.09%. The highest PR percentage was produced by inseminator 6 with a percentage of 50%, while inseminator 2 produced the lowest PR percentage of 13.79%. The average PR percentage of the 6 inseminators was 31.67%. The highest S/C value was produced by inseminators 2, 3, and 5 with an S/C value of 1, while the lowest S/C value was produced by inseminator 4 with a value of 1.33. The average S/C value of the 6 inseminators was 1.41. The inseminator success rate was obtained by inseminator 6 with a value of 50%, while the lowest inseminator success rate was obtained by inseminator 3 with a value of 13.63%. The average inseminator success rate was 23.72%. The conclusion of this study is that the success of artificial insemination by different inseminators is influenced by differences in skills in insemination, insemination time, semen thawing, and experience in artificial insemination.
Keywords: Artificial Insemination, Friesian Holstein, Inseminators,
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| Corresponding Author (Wahyu Yudha Setiawan)
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| 93 |
Animal Reproduction and Breeding |
ABS-90 |
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Multivariate Analysis of Physiological Traits and Thermal Response in Bali Cattle
James Wisnu Arya Tama (1), V.M.A. Nurgiartiningsih (1*), and I. Novianti (1)
Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
Abstract
Bali cattle are native to Indonesia and are highly adaptable to tropical environments. However, fluctuations in temperature and humidity can affect their physiological responses. This study aims to identify the main physiological traits that contribute to the adaptation of Bali cattle to hot tropical conditions in East Kalimantan. A total of sixty-three Bali cattle, including males, non-pregnant females, and pregnant females, were intensively raised and analyzed using a descriptive approach, correlation test, Least Significant Difference (LSD) test, and Principal Component Analysis (PCA). The observed parameters included body surface temperature, Eye Temperature (ET), Forehead Temperature (FT), Dorsal Temperature (DT), Rump Temperature (RT), Respiration Rate (RR), Heart Rate (HR), and Rectal Temperature (RCT). Descriptive analysis showed that respiratory rate had the highest coefficient of variation (CV = 26.15%), indicating high sensitivity to changes in temperature and humidity, while rectal temperature was the most stable (CV = 1.58%). The LSD test showed that bulls had the highest RR and HR values (p < 0.05- p < 0.001), while pregnant cows showed greater inter-individual physiological variation. Correlations between parameters showed a strong relationship between surface temperature and respiratory rate, but a weak relationship between body temperature and respiratory rate (r < 0.2). PCA analysis could identify two principal components explaining 55.2% of the total variation. PC1 represents the external body temperature component, while PC2 represents the internal physiological response (RR, HR). These results indicate that a combination of physiological factors and reproductive status influences Bali cattle adaptation to heat stress. These results provide a basis for developing precision selection strategies and environmental management in local cattle farming systems.
Keywords: Bali cattle, environmental, physiological status, body temperature
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| Corresponding Author (James Wisnu Arya Tama)
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| 94 |
Animal Reproduction and Breeding |
ABS-91 |
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Optimizing Body Weight Prediction in Bali Cattle Using Morphometric Traits and Principal Component Regression
Graciano Lucky Scovier (1*), Karin Sopamena (1), Veronica Margareta Ani Nurgiartiningsih (2), Marjuki (2)
1) Master Scholar of Animal Sciences, Universitas Brawijaya, Malang 65145, Indonesia
*gracianoscovier[at]gmail.com
2) Faculty of Animal Sciences, Universitas Brawijaya, Malang 65145, Indonesia
Abstract
Keywords: Bali cattle, body weight prediction, correlation, regression
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| Corresponding Author (Graciano Lucky Scovier)
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| 95 |
Animal Reproduction and Breeding |
ABS-97 |
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Semen Quality and Bacterial Load in Frozen Semen of Simmental Bull Using Different Diluents
Binti Umi Nurfadilah1, Trinil Susilawati1, Anny Amaliya2 and Aulia Puspita Anugra Yekti1*
1.Department of Animal Reproduction and Breeding, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
2.Singosari National Artificial Insemination Center, Malang 65153, Indonesia
*auliapay[at]ub.ac.id
Abstract
This study aims to determine the differences in bacterial content and quality of frozen Simmental bull semen with the addition of Tris aminomethane egg yolk and Trico egg yolk diluents. The research material consisted of frozen Simmental bull semen samples produced by BBIB Singosari with two types of diluents, namely Tris aminomethane egg yolk and Trico egg yolk. Tests were conducted on 10 straws to examine spermatozoa quality and 6 straws to examine bacterial load in each diluent. The study used a laboratory experimental method with 10 replicates for spermatozoa quality testing and 3 replicates for bacterial testing. The results showed that based on the parameters of individual motility, viability, abnormality, concentration, and total motile spermatozoa, there was no significant difference between the use of the two types of diluents (P>0.05). Bacterial testing showed that the Trico egg yolk diluent contained a higher number of Escherichia coli, compared to the Tris aminomethane egg yolk diluentl. Conversely, the number of Mycoplasma spp. was higher in the Tris aminomethane egg yolk diluent compared to the Trico egg yolk diluent, while Staphylococcus spp. and Pseudomonas spp. bacteria were not found in either diluent. Based on this identification, Mycoplasma spp. was the most dominant bacterium, followed by Escherichia coli, while the other two types of bacteria were not detected. Overall, the Trico egg yolk diluent produced better frozen semen quality in terms of motility, viability, abnormality, and total motile spermatozoa, while the Tris aminomethane egg yolk diluent produced higher spermatozoa concentrations. Therefore, the Trico egg yolk diluent can be an effective alternative in maintaining the quality of Simmental cattle semen during the freezing and storage process.
Keywords: Bacterial Contamination- Simmental Bull- Sperm Quality- Tris Aminomethane Egg Yolk- Trico Egg Yolk
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| Corresponding Author (Binti Umi Nurfadilah)
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| 96 |
Animal Reproduction and Breeding |
ABS-99 |
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Development of In vitro Maturation and In vitro Growth Culture Media Using Goat Colostrum
Dinda Hanindia Kusuma Widjaja1, Gatot Ciptadi, Herly Evanuarini, Sri Wahjuningsih
Faculty of Animal Sciences, Universitas Brawijaya
Abstract
Keywords: In vitro Maturation, In vitro Growth, Goat Colostrum, Oocyte, Follicle.
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| Corresponding Author (Dinda Hanindia Kusuma Widjaja)
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