The Effect of Solvent Ratio with Mahkota Dewa Fruit (Phaleria macrocarpa) Extraction Result and Antimicrobial Activity for Escherichia coli and Salmonella sp. Najma Afrahun Nufus 1), Eko Widodo 2), Heli Tistiana 2)
1) Student of Faculty of Animal Scince, Universitas Brawijaya, Malang, Indonesia
2) Lecturer of Faculty of Animal Science, Universitas Brawijaya Malang, Indonesia
Abstract
The research aimed to determine the effect of solvent ratio Mahkota Dewa fruit (Phaleria macrocarpa) on the extraction results as a feed additive to determine the antimicrobial activity of Escherichia coli and Salmonella Sp. The materials used are fresh Mahkota Dewa fruit (Phaleria macrocarpa) and aquadest. The extraction method used in this study is Ultrasound-Assisted Hydrodistillation Extraction (UAHDE). The antimicrobial activity in this study is the inhibition zone of Mahkota Dewa fruit extract against Lactic Acid Bacteria, Escherichia coli and Salmonella Sp using the agar well diffusion method. The results showed that with an extract ratio of 1: 2 produced a yield of (87.3 ml), 1: 4 (362 ml), and 1: 6 (486 ml). Comparison of the ratios of 1: 4 and 1: 6 showed bacterial inhibition, especially against salmonella, using a concentration of 100% with an inhibition zone of 1:4 (3.55 mm) and 1:6 (3.24 mm) and Lactic Acid Bacteria could not be killed. It can be concluded that the extract of the mahkota dewa fruit have the potential to be used as a feed additive.
