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61 Animal reproduction and breeding ABS-32

The Effect of the Combination of Lactated Ringer^s Solution and Acetated Ringer^s Solution with Egg Yolk and Skim Milk as Cryoprotectants on Abnormalitas and Plasma Membrane Integrity of Chicken Spermatozoa
Fairus Vavang Fisabilillah1) Khaeruddin2) Sri Wahjuningsih3)

1 Student Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia
2 Lecturer Program of Animal Science, Faculty of Agriculture, Universitas Muhammadiyah Sinjai
3 Lecturer Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia


Abstract

This study aims to evaluate the effect of the combination of Ringer^s lactate and Ringer^s acetate diluents supplemented with egg yolk and skim milk as cryoprotectants on abnormalities and plasma membrane integrity of Gaga chicken spermatozoa post-cryopreservation. The study design used a Completely Randomised Design (CRD) with four treatments and ten replicates, including Ringer^s lactate + 10% egg yolk (P1), Ringer^s acetate + 10% egg yolk (P2), Ringer^s lactate + 6% skim milk (P3), and Ringer^s acetate + 6% skim milk (P4). The research procedure included semen collection, semen dilution, pre-freezing and freezing of semen, and thawing. The variables observed were the percentage of abnormalities and plasma membrane integrity of spermatozoa before and after freezing-thawing. Data were analysed using analysis of variance (ANOVA). Based on the results of this study, the use of Ringer^s lactate and Ringer^s acetate diluents combined with 10% egg yolk or 6% skim milk as cryoprotectants did not result in differences in sperm abnormalities and plasma membrane integrity in Gaga chickens during the freezing and thawing process.

Keywords: Gaga chicken, cryopreservation, abnormalities, plasma membrane integrity, Ringer^s lactate, Ringer^s acetate, egg yolk, skim milk.

Share Link | Plain Format | Corresponding Author (Fairus Fisabilillah)


62 Animal reproduction and breeding ABS-33

The Effect of Adding Moringa Leaf Extract to TCG Medium on the Post-Thawing Sperm Quality of Donggala Bull.
Yohan Rusiyantono1, Mohammad Ilyas Mumu1, Mobius Tanari1, Indriani1, Moh. Asril Adjis1

1Animal Husbandry Study Program, Faculty of Animal Husbandry and Fisheries, Tadulako University


Abstract

This study aimed to evaluate the effect of supplementing Moringa oleifera leaf extract to Tris-Citric Egg Yolk (TCG) diluent on Donggala bull post-thawing semen quality. Three bulls fresh semen were allocated into 4 treatments (P0: control, P1: 0.5 mg/mL, P2: 1.0 mg/mL, P3: 1.5 mg/mL Moringa leaf extract), frozen in liquid nitrogen, and evaluated for quality after thawing. ANOVA analysis results showed that the treatment with 1.0 mg/mL Moringa leaf extract (P2) was optimal in motility (58.7+-2.8%), viability (66.4+-3.0%), and normal morphology (82.3+-2.5%). Conclusion: The 1.0 mg/mL Moringa leaf extract treatment is effective to improve post-thawing sperm quality of Donggala bull.

Keywords: Donggala bull, Moringa Leaf Extract, TCG, Post-thawing, Sperm

Share Link | Plain Format | Corresponding Author (Yohan Rusiyantono)


63 Animal reproduction and breeding ABS-45

Sperm Quality in Cryopreserved Holstein Friesian Bull Semen and Bacterial Load as Affected by Different Extenders
Anggita Dian Pramudhita1, Putri Utami1, Habib Asshidiq Syah1, Arik Yuswati1, Asri Nurul Huda2, Anny Amaliya3, Deny Sulistyowati3, Lilik Eka Radiati4, Kuswati5, Trinil Susilawati1 and Aulia Puspita Anugra Yekti1*

1Department of Animal Reproduction and Breeding, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
2Department of Animal Nutrition, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
3Singosari National Artificial Insemination Center, Malang 65153, Indonesia
4Departement of Animal Product Technology, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
5Department of Animal Production, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
*auliapay[at]ub.ac.id


Abstract

This study compared the effects of two different extenders, tris aminomethane egg yolk and modified trico egg yolk, on post-thaw sperm quality and bacterial load in cryopreserved semen from a Holstein Friesian bull. Semen samples were divided into two treatment groups, each consisting of five semen straws (0.25 ml), which were cryopreserved using a Tris aminomethane egg yolk or Trico egg yolk extender. Post-thaw evaluations included sperm motility, viability, abnormality, membrane and acrosome integrity, capacitation status, DNA fragmentation, sperm kinematics, and bacterial load. Data were analyzed using paired t-tests and bacterial content was analyzed descriptively. The results showed that the Tris aminomethane extender significantly enhanced several sperm quality parameters post-thawing compared to Trico egg yolk. Specifically, Tris aminomethane significantly improved intact acrosome (70.36%) and increased the percentage of non-capacitated spermatozoa (67.92%) Furthermore, significantly higher values were observed in parameters related to sperm kinematics, including Distance Average Path (DAP, 33.38%), Distance Curvilinear (DCL, 62.22%), Velocity Average Path (VAP, 84.31%), and Curvilinear Velocity (VCL, 155.06%). Meanwhile, trico egg yolk extender caused significantly higher values in sperm straightness (STR, 84.02%), linearity (LIN, 56.22%), and wobble (WOB, 65.40%). Other parameters, including viability, abnormality, total motile sperm, DNA fragmentation, sperm kinematic parameters (motility, progressive motility, straight-line distance, linear velocity, lateral head amplitude, and beat cross frequency) did not differ significantly between the two extenders. Meanwhile, the bacterial of E. coli and Mycoplasma was higher in the trico extender, while Staphylococcus was more prevalent in tris aminomethane extender. These findings indicate that both extenders can maintain the quality of frozen semen. The results of this study show that Trico egg yolk extender is a promising and cost-effective alternative, with comparable performance in several sperm quality, but the effectiveness of bacterial inhibition needs to be re-evaluated in terms of the dosage of antibiotics used.

Keywords: Bacterial contamination, cryopreservation, sperm quality, Tris aminomethane extender, Trico extender.

Share Link | Plain Format | Corresponding Author (Anggita Dian Pramudhita)


64 Animal reproduction and breeding ABS-49

Slaughter Rate of Productive Female Goats and Potential Utilization of Reproductive Organ Waste for Research on Reproduction and Conservation of Genetic Material
Gatot Ciptadi (a*), Mudawamah Mudawamah (b), Drajad Azis Darmawan (a), Khonita Rahma Diani (c) Zulfikar Ahmad Al Idrus (a), M. Dzamirul Haqqi (a), Yesi Efendi (a), Didik Fudianto (a), Adelina Ari Hamiyanti (a), Agus Budiarto (a), Ardyah Ramadhina Irsanti Putri (a)

(a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
(b) Faculty of Animal Science, Universitas Islam Malang, Malang 65144, Indonesia
(c) Faculty of Mathematics and Natural Science, Universitas Bengkulu, Bengkulu 38119, Indonesia

*ciptadi[at]ub.ac.id


Abstract

This study aims to determine the potential for utilizing the waste reproductive organs of productive female goats slaughtered at Malang Goat slaughterhouses. The method used is a case study. The study was conducted at the Malang City Slaughterhouse. The total livestock sample was grouped by puberty and prepubertal age. Results showed that the slaughter rate of male goats reached about 15%, and that of females reached 85%. The slaughter rate of goat aged less than 1 year (prepubertal) is 28,91%. While the livestock puberty rate with an age range of 1 - 1,5 years is about 23%, 1.5 - 2,5 years old is 13%, 2,5 - 3,5 years old is 14 % and 3,5 - 4 years old is 21%. Ovarian reproductive organ waste has the potential to provide oocytes that can be used for research and conservation of genetic material by freezing cells in liquid nitrogen (LN2). Prepubertal ovaries are not feasible for oocyte maturation culture, while pubertal ovaries can isolate oocytes for in vitro culture and frozen storage for conservation. The conclusion is that female goat slaughter at Malang slaughterhouse is dominated by female goats, reaching about 85%. The highest goat slaughter rate aged less than 1 year reached 28%. It is suggested to reduce the number of slaughtered does, and further research is needed on the level of productive does slaughtered.

Keywords: Goat- Slaughter Rate- Conservation- Reproduction- Genetic

Share Link | Plain Format | Corresponding Author (Drajad Azis Darmawan)


65 Animal reproduction and breeding ABS-57

The Protective Effects of Astaxanthin Supplementation in Tris- Aminomethane on the Sperm Quality of Merino Ram During Cold Storage
Amelia Nanda Vidyaningrum1, Elok Putri Agustina1, Asrullah As1, Wike Andre Septian1, Rafika Febriani Putri1, Chairdin Dwi Nugraha2, Ari Ardiantoro1, Achadiah Rachmawati1, and Suyadi Suyadi1

1Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia
2Research Center for Animal Husbandry, National Research and Innovation Agency Republic of Indonesia (BRIN), Bogor 16911, Indonesia


Abstract

Ram spermatozoa are particularly susceptible to lipid peroxidation induced by reactive oxygen species (ROS) during cold storage. Astaxanthin a potent carotenoid antioxidant, is known for its ability to neutralize free radicals and protect sperm cells from oxidative stress. This study aimed to evaluate the effect of astaxanthin supplementation in a Tris-aminomethane egg yolk extender on the semen quality of Merino ram during cold storage. Fresh semen was collected from a healthy, sexually mature Merino ram housed in a colony system at the Sumber Sekar Field Laboratory using an artificial vagina. Only ejaculates with individual motility &#8805-70%, viability &#8805-70%, abnormal morphology <10%, and membrane integrity &#8805-70% were selected. Semen samples were diluted using Tris-aminomethane egg yolk extender and divided into four treatment groups supplemented with astaxanthin (1 &#956-M, 2 &#956-M, 3.5 &#956-M, and 4.5 &#956-M). The diluted semen samples were cooled gradually and preserved at 5&#730-C for three days. Sperms in chilled diluted semen samples were assesment for motility, viability, abnormality and plasma membrane integrity every 24 h for three days (72 h). A completely randomized design with eight replicates per treatment was applied. The results showed that spermatozoa remained viable and potentially fertilizing up to 72 hours of storage, as indicated by motility rates above 40% in all groups. Supplementation with 3.5 &#956-M astaxanthin significantly improved (P<0.05) motility, viability, reduced abnormal morphology, and maintained plasma membrane integrity. In conclusion, although all astaxanthin doses positively affected semen quality, supplementation at 3.5 &#956-M demonstrated the most consistent biological response in preserving sperm quality during 72 hours of cold storage.

Keywords: Astaxanthin, ROS, Merino Ram, Sperm Quality, Cold Storage

Share Link | Plain Format | Corresponding Author (AMELIA NANDA VIDYANINGRUM)


66 Animal reproduction and breeding ABS-70

Morphometric Analysis and Breeding Selection Potential of Tropical Swamp and River Buffalo in Indonesia
Wari1, V.M.A. Nurgiartiningsih1, and I. Novianti1*

1Faculty of Animal Science, Brawijaya University, Malang 65145, Indonesia
*wariwaridoang[at]student.ub.ac.id


Abstract

Buffalo are an important contributor to meat production in Indonesia, yet their population has been declining, requiring structured selection and breeding strategies. This study analyzed morphometric traits of swamp and river buffalo from Bahal Batu, Rondaman Palas, and Silangit, considering the effects of sex, age, season of birth, and breeding region. Statistical analyses included ANOVA, t-tests, and principal component analysis (PCA). Results showed that age significantly influenced body weight (F = 34.25, p < 0.001), body length (F = 18.72, p < 0.001), chest girth (F = 12.14, p < 0.01), and withers height (F = 9.65, p < 0.01). Sex differences were also significant, with males outperforming females in body weight (5.39 vs 5.16- p = 0.016), chest girth (5.01 vs 4.93- p = 0.008), and withers height (113.4 vs 107.4 cm- p = 0.013). Season of birth and breeding region contributed to variation (p < 0.05), with wet-season-born and Silangit buffalo showing superior growth. PCA revealed two principal components explaining 85% of variation, mainly associated with body weight and chest girth (PC1) and body length and withers height (PC2). These results highlight the importance of morphometric evaluation combined with fixed-effect analysis to strengthen breeding selection and improve buffalo productivity in tropical regions.

Keywords: tropical buffalo, swamp buffalo, river buffalo, morphometric traits, breeding selection

Share Link | Plain Format | Corresponding Author (Wari Wari)


67 Animal reproduction and breeding ABS-71

The Implementation of SMS Gateaway System in Improving Dairy Cattle Recording
Sucik Maylinda1), Hari Dwi Utami1), Raden Arief Setyawan2), Sarwono3)

1) Faculty of Animal Science, Universitas Brawijaya
2) Faculty of Engineering, Universitas Brawijaya
3) Faculty of Administrative Science, Universitas Brawijaya


Abstract

Dairy farming in Indonesia it is very difficult to find Production and Reproduction Recording. Recording is an important element in animal husbandry in developed countries, in which case the main activity is (1) measurement (in the measuring volume or weighing body weight). (2) measuring milk production (3) recording the date of the cow^s mating month, (4) ) measures other performances besides milk production such as birth weight, cow weight, one year weight, body weight gain. The measured performance is collected in an activity called a recording activity. Recording activities include three stages, namely: A. Data collection through measuring data in the field by weighing, measuring milk volume, measuring growth data and body measurements such as chest circumference, height, body length. Input data into a data tabulation. C. Input can be continued to a data analysis program. All of these stages can be done offline and online. In the current millennial era, almost all activities can be done online, including recording programs. This includes implementing the SMS GATEWAY Recording system. The SMS GATEWAY Recording System is a dairy cattle production recording system that combines data collection, data tabulation with data sending via SMS and sent to a data center and connected to data analysis software. The Doctoral Service Project has been implemented from June 12 2023 to September 12 2023 in Sebaluh Village, Pandesari Pujon. The team collaborates with the Agriconservative Breeders Group led by Mr. Ali Mahmud, SPt, MP. The outcomes achieved are as follows: I. Formation of a community of breeders ^Sadar Recording^. This begins with the socialization of this activity at the start of the activity, namely around the beginning of June 2023. The socialization activity is held at the home of the Head of the Research Team, namely Prof. Dr. Ir. Sucik Maylinda, MS in Singosari. Documentation of these activities is as follows. This activity was attended by 8 breeders. In this activity, breeders were given awareness about the importance of ^recording^. In this activity, the program ^Production Recording Via SMS Gateway Software^ was also introduced. II. Implementation of Activities. Implementation of this activity begins with the creation of SMS Gateway Recording Software with the SMS Gateway software writing format with the following link http://sucik.iddevops.com/

Keywords: Dairy cows, Production records, Online recording, SMS Gateway

Share Link | Plain Format | Corresponding Author (Sucik Maylinda)


68 Animal reproduction and breeding ABS-77

Morphometric Characterization of Cross Texel, DEG, and Dorper Sheep Using Principal Component Analysis
Muhammad Pramujo1, Luqman Hakim1, Sucik Maylinda1*

1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia


Abstract

The objective of this study was to identify the dominant morphometric traits that characterize Cross Texel, Fat Tail Sheep (DEG), and Dorper Full Blood ewes aged 24 months using Principal Component Analysis (PCA). The research method applied was field observation. The material consisted of 85 ewes, including Cross Texel (34 heads), DEG (31 heads), and Dorper Full Blood (20 heads). The observed variables were chest circumference (LD), body height (TB), body length (PB), and body weight (BB). Data were analyzed using descriptive statistics, Pearson correlation, and PCA. Correlation analysis showed a strong positive relationship among traits in Cross Texel and DEG, with the highest correlation between BB and LD in DEG (0.835). PCA results indicated that Cross Texel and DEG populations were mainly explained by a single principal component, which accounted for 66.59% and 81.91% of the total variance, respectively. This component was dominated by BB (0.951 in DEG) and LD (0.826 in Cross Texel), suggesting that overall body size was the primary differentiating factor. In Dorper Full Blood, two principal components explained 85.74% of the total variance. PC1 (56.16%) was associated with LD (0.929) and BB (0.918), whereas PC2 (29.57%) was primarily influenced by PB (0.946), indicating variation in body proportion. These findings demonstrate that body weight and chest circumference are the main indicators of size in Cross Texel and DEG, while Dorper exhibits more complex variability involving both body mass and length.

Keywords: Cross Texel, DEG, Dorper Full Blood, Body Size, Principal Component Analysis (PCA)

Share Link | Plain Format | Corresponding Author (Muhammad Pramujo)


69 Animal reproduction and breeding ABS-79

The Effect of Different Types of Commercial Diluents on The Semen Quality of Polled Bali Bull
Andi Rezda Sri Mulyani(1), Ronny Markus Jami Riwu(2), Lutfiah Aminah(3), Siti Nuraisyah Hamsir(3), Sudirman Baco(3), Herry Sonjaya(3), and Hasbi Hasbi(3*)

(1)Student of Animal Science, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
(2)Department of Animal Science, Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University, Kupang 85228, Indonesia.
(3)Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia
*hasbi_fapetunhas[at]yahoo.com


Abstract

Polled Bali cattle are a variant of Bali bull characterized by the natural absence of horn growth. In artificial insemination programs, Andromed is a commonly used commercial semen diluent- however, limited studies have evaluated its performance compared to other diluents containing lecithin based components. This study aimed to assess the semen quality of polled Bali bull using three commercial diluents: Andromed, Steridyl, and Bovifree. The research was conducted at the UPT PIBPS, Livestock and Animal Health Service of South Sulawesi Province, and the In Vitro Embryo Production Laboratory, Hasanuddin University. Semen was collected from two polled Bali bulls aged 5 and 7 years. A completely randomized design (CRD) was employed with three treatment groups and seven replications. Semen quality was evaluated based on progressive motility, viability, abnormalities, and Intact Plasma Membrane (IPM). Data were analyzed using analysis of variance (ANOVA) followed by Duncans multiple range test. The results indicated that Andromed significantly improved sperm motility (P<0.01) compared to Steridyl and Bovifree. Viability did not differ significantly among the diluents (P>0.05). Sperm abnormalities were significantly higher in the Steridyl group (P<0.01), while Andromed showed significantly better plasma membrane integrity than Steridyl (P<0.05), but was not substantially different from Bovifree (P>0.05). In conclusion, Andromed demonstrated better performance in most semen quality parameters compared to Steridyl and Bovifree for polled Bali bulls semen cryopreservation.

Keywords: Commercial diluent, Frozen semen, Polled Bali bull, Semen quality

Share Link | Plain Format | Corresponding Author (Andi Rezda Sri Mulyani)


70 Animal reproduction and breeding ABS-81

Comparative Field Evaluation of Sexed and Conventional Semen in Holstein-Friesian Cows: Insights into Semen Quality, Fertility Performance, and Calf Sex Ratio Across Diverse Environments
Putri Utami1, Aulia Puspita Anugra Yekti1, Anggita Dian Pramudhita1, Habib Asshidiq Syah1, Tri Agus Siswoyo2, Nurul Isnaini1 and Trinil Susilawati1*

1Department of Animal Reproduction and Breeding, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
2Graduate Program of Biotechnology, the Center of Excellence on Crop Industrial Biotechnology (PUI-PT BioTIn), Universitas Jember, Jember 68121, Indonesia.
*tsusilawati[at]ub.ac.id


Abstract

The use of sexed semen in dairy cattle breeding has been widely promoted to accelerate herd replacement by increasing the proportion of female calves, yet its effects on semen quality, fertility outcomes, and sex ratios under diverse environmental conditions remain insufficiently understood. This study evaluated semen quality, reproductive performance, and calf sex ratios of Holstein-Friesian cows inseminated with sexed and conventional semen across three dairy farms with different environmental conditions, involving 300 cows in total. Semen was analyzed for motility, viability, sperm concentration, abnormalities, plasma membrane integrity, acrosome integrity, and DNA fragmentation, while fertility was assessed through non-return rate, conception rate, calving rate, and calf sex ratio. Statistical analyses were conducted using independent t-tests for semen parameters and Chi-square/Fishers Exact Test for reproductive outcomes. The results showed that sexed semen maintained comparable motility to conventional semen but exhibited significantly lower viability, sperm concentration, plasma membrane integrity, and acrosome integrity (p < 0.05), whereas abnormalities, total motile sperm, and DNA fragmentation did not differ significantly (p > 0.05). Field fertility performance did not show significant differences between treatments at any location (p > 0.05), although Ngantang presented the highest trend, with conception and calving rates of up to 44%. In conclusion, sexed semen provides reproductive performance comparable to conventional semen under field conditions, despite certain reductions in quality attributes, supporting its application as a practical strategy to increase female calf production, with further long-term and multi-location studies needed to optimize outcomes under tropical dairy systems.

Keywords: conception rate, Holstein-Friesian, semen quality, sexed semen, sex ratio

Share Link | Plain Format | Corresponding Author (Putri Utami)


71 Animal reproduction and breeding ABS-86

Ovarian morphometric characterization of Philippine Native and Upgraded Goats
Aubrey Joy M. Balbin*, Jonathan N. Nayga1, and Maria C. Hernandez2

1 Cagayan Valley Small Ruminant Research Center, Isabela State University (ISU-CVSRRC), Echague, Isabela 3309 Philippines

2 Quirino State University, Diffun, Quirino 3401 Philippines


Abstract

Ovarian morphometric characterization is an important data in livestock production used for improving reproductive efficiency, predicting fertility, and guiding biotechnology applications particularly on embryo transfer. This study was conducted to establish ovarian structure characterization of Philippine Native and Upgraded goats. There are 50 samples collected from the slaughterhouse of ISU-CVSRRC, equally represented by each breed. After collection, the samples were kept in saline solution maintained at 36 C and transported to the laboratory The ovaries were dissected to evaluated parameters such as weight (g), length (cm), width (cm) and circumference (cm) of the ovary and number of dominant follicles. The analysis shows that between breeds, the mean difference in weight, circumference, and number of follicles significant (p < 0.05). It was also noted that within breeds, the mean difference of parameters between the right and left ovary also varies. Specifically in terms of length of ovary, the recorded mean varies significantly (p < 0.05) in upgraded does. On the number of follicles, it was noted that there is significant difference, noting that in Philippine native goats, more follicles are noted in right ovary at 17.6 vs. 17.1 in left ovary- while in Upgraded goats, there are at least 15.9 follicles obtained in left ovary vs 15.4 in right ovary.

Keywords: Goat ovary, morphometric characterization, follicle

Share Link | Plain Format | Corresponding Author (Aubrey Joy Balbin)


72 Animal welfare ABS-2

Significance of livestock in Maharashtrian Culture
Prof. Dr. Vijaykumar Shivdas Dhole

Sanjeevanee Mahavidyalaya (College), Chapoli
Tq. Chakur Dist. Latur (MS) INDIA


Abstract

The diverse livestock in Maharashtra is not only economic wealth but also cultural heritage. The nomadic Dhangar and other communities have preserved this tradition for generations. Diverse livestock is the true identity of Maharashtra- from farmers to the nomadic communities, everyone has raised various pets according to their needs. Farmers have kept different breeds of cows, buffaloes, goats, hens, dogs, and cats. Similarly, those involved in earthwork such as laborers and potters have raised donkeys as versatile animals, while stone workers and other tribal communities are often seen with pigs. The Dhangar community can still be found migrating with their wealth in the form of sheep, goats, cows, dogs, horses, and hens. These communities have significantly contributed to preserving the indigenous livestock of Maharashtra. The tribal communities in Gadchiroli and Gondia maintain their indigenous cattle and lineage. For generations, farmers of all castes and religions are still seen maintaining local livestock, and the Brahmin community is no exception. On the other hand, the nomadic and settled livestock keepers in the state primarily rear Osmanabadi, Sangamneri, Surti and Konkani goats, as well as Dakhini and Madgyal sheep. Balu Mama^s sheep are famous, with a total of 17 flocks spread across various locations.

Keywords: Livestock, Cultural, Maharashtra, Nomadic tribe

Share Link | Plain Format | Corresponding Author (Vijaykumar Shivdas Dhole)


73 Animal welfare ABS-3

The Role of Administration in Animal Welfare
Dr. Santram Prabhakar Mundhe

Deptt. of Public Administration, Sanjeevanee Mahavidyalaya, Chapoli
Tq. Chakur Dist. Latur (MS)INDIA


Abstract

Animal welfare refers to the ethical and practical responsibility humans have to provide for animals^ well-being. It includes protecting animals from unnecessary suffering, providing veterinary care, and ensuring that animals can express natural behaviors including food and shelter. Animals in agricultural settings, proper welfare practices contribute to better health, which in turn leads to better productivity. Stress and poor living conditions can lead to health issues, reduced lifespan, and, in some cases, the spread of disease. Administration plays very important role in taking care of these animals. The administration makes laws and rules to ensure the health, safety and welfare of animals and it is the duty of the administration to implement them and create awareness. From the present investigation it is concluded that making laws and rules is very important to prevent cruelty to animals and for their welfare. Strict implementation of laws and strict action is necessary in case of their violation. It is equally important to explain to the people the importance of animal welfare, as well as to inform them about their rights and to tell them the importance of nature conservation. The administration is responsible for the implementation of the Animals. Cases of cruelty are investigated through the Police and the Forest Department. Animal Welfare Boards are institutional mechanisms that work for the health, protection and welfare of livestock. The boards take care of the health of livestock. It includes vaccination, prevention and health check-ups.

Keywords: Animal Welfare, Administration, Ethical

Share Link | Plain Format | Corresponding Author (Santram Prabhakar Mundhe)


74 Animal welfare ABS-9

Animal Welfare Assessment at the Horse Market in Jeneponto Regency
Ayu Lestari

Department of Animal Science, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar

Jl. H.M. Yasin Limpo No. 36, Samata, Gowa Regency, South Sulawesi


Abstract

The horse market in Jeneponto is the only livestock market place in Indonesia that specifically markets horses. Horses that will be traded are brought to the market in the morning to be sold and if the horse does not sell then the seller takes it home again in the afternoon. The condition of the horse market with limited facilities is thought to affect the welfare of horses while in the market. This study was conducted at the Horse Market, located in Kelara District, Jeneponto Regency. The study aims to assess the implementation of horse welfare while at the horse market location. Research type is qualitative descriptive research. The research instrument used was a questionnaire adapted from the guidelines for fostering the implementation of animal welfare and guidelines for the implementation of animal welfare by the Ministry of Agriculture of the Republic of Indonesia. There are 5 (five) aspects observed in this study, namely: 1. Feed and drink- 2. Cages and equipment- 3. Health- 4. Human resources- 4. Animal comfort and safety- and 5. Livestock recording. The results of the study indicate that the aspects that have been met are the aspects of the cage and equipment, namely adequate ventilation, the aspects of animal comfort and safety, namely the appropriate herding process, and the aspect of livestock recording, namely the existence of data records of the identity of horse livestock. It can be concluded that the horse market in Jeneponto Regency has not fulfilled the implementation of livestock welfare from all aspects of feed and drink, health, and human resources.

Keywords: animal welfare, assessment, horse, market.

Share Link | Plain Format | Corresponding Author (Ayu Lestari)


75 Animal welfare ABS-14

A COMPARATIVE STUDY OF HEMATOLOGICAL PROFILES AND Mycoplasma gallisepticum PREVALENCE IN PHILIPPINE NATIVE CHICKENS RAISED UTILIZING CPU TECHNOLOGY AND BACKYARD FREE-RANGE METHODS
Allyssa Depasupil and Jaime C. Cabarles, Jr.

Central Philippine Unversity


Abstract

Philippine Native chickens are typically raised using a free-range system in backyards. The management style is characterized by its low-input, low-output nature. To address the growing demand for native chicken, production is intensified through the strategic use of available feed resources, occasionally supplemented with commercial feed additives. Native chickens exhibit notable disease resistance and adaptability to local climatic conditions, making them more favored by local communities. However, these free-ranging habits expose them to vulnerabilities related to diseases and nutritional deficiency. In response to the identified challenges, Central Philippine University developed a technological package for raising native chickens. This innovative approach features confined housing and employs an antibiotic-free feeding management, besides the use of phytomedicines. An evaluation of this technology was conducted, focusing on the hematological profiles and the prevalence of Mycoplasma gallisepticum in native chickens. The findings revealed that 73% of the sample population of backyard chickens tested positive for M. gallisepticum infection, whereas chickens raised under CPU technology exhibited only a 6% positive rate. Both hematological and antibody test results corroborated these findings, allowing for effective disease evaluation. Notably, the free-range chickens displayed elevated Mean Corpuscular Volume (MCV) values compared to standard reference ranges. Hence, this technological application significantly reduced the incidence of Mycoplasma gallisepticum in native chickens, addressing a respiratory disease that leads to an alteration in erythrocyte size.

Keywords: Hematological Analysis, Mycoplasma gallisepticum, Philippine Native Chicken

Share Link | Plain Format | Corresponding Author (Allyssa Depasupil)


76 Animal welfare ABS-15

SURVIVAL RATE, FECALYSIS, HEMATOLOGICAL AND BLOOD CHEMISTRY OF PHILIPPINE NATIVE CHICKEN SUPPLEMENTED WITH ESSENTIAL OILS
Jaime C. Cabarles Jr., Marielle Faye M. Cajayon, and Allyssa M. Depasupil

Central Philippine University


Abstract

This systematic review evaluated the effects of various essential oils on the survival rate, fecalysis, hematology, and blood biochemistry of Philippine native chickens. A total of twelve experimental studies conducted by agriculture students from Central Philippine University in 2024 were included. The essential oils investigated-derived from Rosemary, Eucalyptus, Turmeric, Moringa, Hauili, Guava, Galangal, Jatropha, Lemon, Betel, Oregano, and Neem-were administered orally alongside virgin coconut oil. Across all treatments, essential oils were found to be safe, with no reported mortality or acute toxicity. Several oils, particularly Neem, Hauili, Guava, Jatropha, Lemon, and Galangal, demonstrated significant anthelmintic activity by reducing or eliminating parasitic eggs in fecal samples. Hematological analysis revealed improvements in immune response and erythropoiesis, with increased white and red blood cell parameters in many treatments. Biochemical findings showed varied responses, with Moringa and Turmeric essential oils notably improving liver function and metabolic markers, while Hauili, Galangal, and Jatropha raised potential concerns for liver and kidney stress at tested doses. Overall, the findings support the potential of plant-derived essential oils as sustainable alternatives to synthetic drugs in poultry production. Further research is recommended to refine dosage, ensure long-term safety, and evaluate economic viability for broader application in organic poultry farming.

Keywords: Fecalysis, Hematological analysis, Blood chemistry, Philippine Native Chicken, Supplements

Share Link | Plain Format | Corresponding Author (Allyssa Depasupil)


77 Animal welfare ABS-28

SENSITIVITY, SPECIFICITY, AND ACCURACY EVALUATION OF A COLORIMETRIC DETECTION KIT FOR Mycoplasma gallisepticum IN CHICKENS USING GREEN SYNTHESIZED GOLD NANOPARTICLES AND IGY CONJUGATES
Allyssa Depasupil, Neil Francis Vicentino, Lhyssa Marie Dobleo, Karen Jee Fernandez, and Jaime Cabarles, Jr.

Central Philippine University


Abstract

Mycoplasma gallisepticum is a significant pathogen affecting poultry production, causing chronic respiratory disease and substantial economic losses. This study evaluated a colorimetric detection kit for M. gallisepticum using green-synthesized gold nanoparticles (AuNPs) from cashew leaves conjugated with immunoglobulin Y (IgY) antibodies. The kit was field-tested on 20 chickens and compared with microbial culture results. The detection kit demonstrated 100% specificity and positive predictive value, indicating its ability to accurately identify true positive cases. However, it showed limited sensitivity (52.38%) and accuracy (54.55%), with a low negative predictive value (9.09%). The kit provided rapid results within 30 minutes compared to 24 hours for microbial testing and was cost-effective at 15.00 PHP per test. While the kit successfully detected subclinical infections, its performance could be improved through further optimization of antibody-antigen binding and larger-scale field testing. Integrating the rapid colorimetric test with confirmatory microbial testing is recommended for enhanced reliability in field settings. Future research should focus on adapting this technology for other poultry pathogens and assessing its economic impact on poultry farming operations.

Keywords: Mycoplasma gallisepticum, Poultry, Colorimetric Detection kit, Gold Nanoparticles, IgY Antibodies

Share Link | Plain Format | Corresponding Author (Allyssa Depasupil)


78 Animal welfare ABS-89

SEROLOGICAL, WHITE BLOOD CELLS, AND RED BLOOD CELLS ANALYSIS TO DETERMINE AVIAN REOVIRUS SEROPREVALENCE IN NATIVE CHICKENS IN ILOILO PROVINCE
Christel C. Biatan, Allyssa M. Depasupil, Miguel M. Casumpang, and Jaime C. Cabarles Jr.

Central Philippine University


Abstract

Avian reovirus (ARV) is a viral infection that significantly affects large populations of chickens globally. The most notable illnesses associated with this reovirus in chickens include tenosynovitis/arthritis, immunosuppression, gastrointestinal disease, and runting-stunting syndrome. The presence of ARV can undermine the productivity of chickens and adversely influence their overall health, mobility, and mortality rates. This study investigated the serological identification, hematological analysis, and determined the seroprevalence rate of avian reovirus in native chickens from selected barangays in two municipalities characterized by high native chicken populations. Out of the 72 samples tested, 54 were identified as positive for reovirus, with 20 of these cases being asymptomatic, underscoring the importance of early diagnosis. The notably high seroprevalence rate of 75% shows an urgent need for enhanced biosecurity measures, rapid detection techniques, and comprehensive vaccination campaigns to mitigate the detrimental effects of ARV infection on chicken health and the sustainability of the local economy.

Keywords: Avian Reovirus, Native Chicken, Serolical Analysis, Red blood cells, White blood cells, Serprevalence

Share Link | Plain Format | Corresponding Author (Allyssa Depasupil)


79 Integrated farming system ABS-66

Unveiling Food Insecurity Status of Cattle Farm Household Living Adjacent to Teak Forest Region in East Java, Indonesia: A Preliminary Study
Khofifa Dwi Hafsari, Priyo Sugeng Winarto*, Eko Nugroho

Universita Brawijaya


Abstract

This preliminary study investigates the food insecurity status and livelihood strategies of cattle farming households living in the buffer zones of Bojonegoro^s teak forests, East Java, Indonesia. A total of 100 respondents were surveyed to analyze household resilience in sustaining food security under socio-economic and ecological pressures. The Food Insecurity Experience Scale (FIES) was employed to classify households into four categories: food secure, mildly food insecure, moderately food insecure, and severely food insecure. The results show that food security status varies across households, reflecting differences in resource access, income diversification, and coping mechanisms. Strategies adopted include diversifying livelihoods, restructuring labor roles, and engaging with local institutions to strengthen adaptive capacity. Key coping mechanisms involve storing agricultural produce for food consumption and maintaining livestock as a form of savings, which enables households to meet their food needs during periods of uncertainty. The findings contribute to a deeper understanding of household-level resilience and provide insights for policy formulation and development interventions that are ecologically grounded, socially inclusive, and contextually relevant within forest livestock agriculture interface communities.

Keywords: cattle-based livelihoods- food security strategies- livestock household economy- rural resilience- sustainable farming practices

Share Link | Plain Format | Corresponding Author (Khofifa Dwi Hafsari)


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