The Effect of Different Types of Commercial Diluents on The Semen Quality of Polled Bali Bull
Andi Rezda Sri Mulyani(1), Ronny Markus Jami Riwu(2), Lutfiah Aminah(3), Siti Nuraisyah Hamsir(3), Sudirman Baco(3), Herry Sonjaya(3), and Hasbi Hasbi(3*)

(1)Student of Animal Science, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
(2)Department of Animal Science, Faculty of Animal Husbandry, Marine and Fisheries, Nusa Cendana University, Kupang 85228, Indonesia.
(3)Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia
*hasbi_fapetunhas[at]yahoo.com

Abstract

Polled Bali cattle are a variant of Bali bull characterized by the natural absence of horn growth. In artificial insemination programs, Andromed is a commonly used commercial semen diluent- however, limited studies have evaluated its performance compared to other diluents containing lecithin based components. This study aimed to assess the semen quality of polled Bali bull using three commercial diluents: Andromed, Steridyl, and Bovifree. The research was conducted at the UPT PIBPS, Livestock and Animal Health Service of South Sulawesi Province, and the In Vitro Embryo Production Laboratory, Hasanuddin University. Semen was collected from two polled Bali bulls aged 5 and 7 years. A completely randomized design (CRD) was employed with three treatment groups and seven replications. Semen quality was evaluated based on progressive motility, viability, abnormalities, and Intact Plasma Membrane (IPM). Data were analyzed using analysis of variance (ANOVA) followed by Duncans multiple range test. The results indicated that Andromed significantly improved sperm motility (P<0.01) compared to Steridyl and Bovifree. Viability did not differ significantly among the diluents (P>0.05). Sperm abnormalities were significantly higher in the Steridyl group (P<0.01), while Andromed showed significantly better plasma membrane integrity than Steridyl (P<0.05), but was not substantially different from Bovifree (P>0.05). In conclusion, Andromed demonstrated better performance in most semen quality parameters compared to Steridyl and Bovifree for polled Bali bulls semen cryopreservation.

Keywords: Commercial diluent, Frozen semen, Polled Bali bull, Semen quality

Topic: Animal reproduction and breeding