The Effect of the Combination of Lactated Ringer^s Solution and Acetated Ringer^s Solution with Egg Yolk and Skim Milk as Cryoprotectants on Abnormalitas and Plasma Membrane Integrity of Chicken Spermatozoa
Fairus Vavang Fisabilillah1) Khaeruddin2) Sri Wahjuningsih3)

1 Student Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia
2 Lecturer Program of Animal Science, Faculty of Agriculture, Universitas Muhammadiyah Sinjai
3 Lecturer Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia

Abstract

This study aims to evaluate the effect of the combination of Ringer^s lactate and Ringer^s acetate diluents supplemented with egg yolk and skim milk as cryoprotectants on abnormalities and plasma membrane integrity of Gaga chicken spermatozoa post-cryopreservation. The study design used a Completely Randomised Design (CRD) with four treatments and ten replicates, including Ringer^s lactate + 10% egg yolk (P1), Ringer^s acetate + 10% egg yolk (P2), Ringer^s lactate + 6% skim milk (P3), and Ringer^s acetate + 6% skim milk (P4). The research procedure included semen collection, semen dilution, pre-freezing and freezing of semen, and thawing. The variables observed were the percentage of abnormalities and plasma membrane integrity of spermatozoa before and after freezing-thawing. Data were analysed using analysis of variance (ANOVA). Based on the results of this study, the use of Ringer^s lactate and Ringer^s acetate diluents combined with 10% egg yolk or 6% skim milk as cryoprotectants did not result in differences in sperm abnormalities and plasma membrane integrity in Gaga chickens during the freezing and thawing process.

Keywords: Gaga chicken, cryopreservation, abnormalities, plasma membrane integrity, Ringer^s lactate, Ringer^s acetate, egg yolk, skim milk.

Topic: Animal reproduction and breeding