The Effect of Adding Moringa Leaf Extract to TCG Medium on the Post-Thawing Sperm Quality of Donggala Bull.
Yohan Rusiyantono1, Mohammad Ilyas Mumu1, Mobius Tanari1, Indriani1, Moh. Asril Adjis1

1Animal Husbandry Study Program, Faculty of Animal Husbandry and Fisheries, Tadulako University

Abstract

This study aimed to evaluate the effect of supplementing Moringa oleifera leaf extract to Tris-Citric Egg Yolk (TCG) diluent on Donggala bull post-thawing semen quality. Three bulls fresh semen were allocated into 4 treatments (P0: control, P1: 0.5 mg/mL, P2: 1.0 mg/mL, P3: 1.5 mg/mL Moringa leaf extract), frozen in liquid nitrogen, and evaluated for quality after thawing. ANOVA analysis results showed that the treatment with 1.0 mg/mL Moringa leaf extract (P2) was optimal in motility (58.7+-2.8%), viability (66.4+-3.0%), and normal morphology (82.3+-2.5%). Conclusion: The 1.0 mg/mL Moringa leaf extract treatment is effective to improve post-thawing sperm quality of Donggala bull.

Keywords: Donggala bull, Moringa Leaf Extract, TCG, Post-thawing, Sperm

Topic: Animal reproduction and breeding