Semen Quality and Bacterial Load in Frozen Semen of Simmental Bull Using Different Diluents Binti Umi Nurfadilah1, Trinil Susilawati1, Anny Amaliya2 and Aulia Puspita Anugra Yekti1*
1.Department of Animal Reproduction and Breeding, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
2.Singosari National Artificial Insemination Center, Malang 65153, Indonesia
*auliapay[at]ub.ac.id
Abstract
This study aims to determine the differences in bacterial content and quality of frozen Simmental bull semen with the addition of Tris aminomethane egg yolk and Trico egg yolk diluents. The research material consisted of frozen Simmental bull semen samples produced by BBIB Singosari with two types of diluents, namely Tris aminomethane egg yolk and Trico egg yolk. Tests were conducted on 10 straws to examine spermatozoa quality and 6 straws to examine bacterial load in each diluent. The study used a laboratory experimental method with 10 replicates for spermatozoa quality testing and 3 replicates for bacterial testing. The results showed that based on the parameters of individual motility, viability, abnormality, concentration, and total motile spermatozoa, there was no significant difference between the use of the two types of diluents (P>0.05). Bacterial testing showed that the Trico egg yolk diluent contained a higher number of Escherichia coli, compared to the Tris aminomethane egg yolk diluentl. Conversely, the number of Mycoplasma spp. was higher in the Tris aminomethane egg yolk diluent compared to the Trico egg yolk diluent, while Staphylococcus spp. and Pseudomonas spp. bacteria were not found in either diluent. Based on this identification, Mycoplasma spp. was the most dominant bacterium, followed by Escherichia coli, while the other two types of bacteria were not detected. Overall, the Trico egg yolk diluent produced better frozen semen quality in terms of motility, viability, abnormality, and total motile spermatozoa, while the Tris aminomethane egg yolk diluent produced higher spermatozoa concentrations. Therefore, the Trico egg yolk diluent can be an effective alternative in maintaining the quality of Simmental cattle semen during the freezing and storage process.
