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Comparison of sperm motility and capacitation status in egg-white sedimentation sexed frozen semen of Holstein-Friesian Bulls
Dhyta Amallia Miranti (1), Trinil Susilawati (1), Sri Wahjuningsih (1), Muhammad Ardhi Firdaus (1), Raysha Nasywana Viola (1), Galih Darmawan Kusuma (1), Arganto Rifqi Satrio (1), Anggita Dian Pramudhita (1), Habib Asshidiq Syah (1), Aidil Agam (1), Koko Wisnu Prihatin (2), Yeni Fitriantini (2), Deny Sulistyowati (2), Anny Amaliya (2), and Aulia Puspita Anugra Yekti (1*)
(1) Faculty of Animal Sciences, Universitas Brawijaya, Malang, 65145, Indonesia
(2) Singosari National Artificial Insemination Center (SNAIC), Singosari, Malang Regency, 65153, Indonesia
Abstract
This study evaluated sperm motility and capacitation status in frozen sexed semen produced using the egg-white sedimentation method in Holstein-Friesian (HF) bulls. Frozen semen was obtained from two HF bulls, VA Master (ID 322126) and GW Amish (ID 322123), across five production batches at the Singosari Artificial Insemination Center (SNAIC), Indonesia. The treatments consisted of non-sexed semen (T0), X-sexed semen (T1), and Y-sexed semen (T2). Post-thaw sperm motility was evaluated using a microscope at 400x magnification, while capacitation status was assessed using chlortetracycline (CTC) staining and classified into three categories: non-capacitated, capacitated, and acrosome-reacted sperm. Data were analyzed using analysis of variance (ANOVA), followed by Duncan^s Multiple Range Test. Sperm motility differed significantly among treatments (p = 0.0476), with values of 43.80 +/- 3.46%, 41.10 +/- 2.13%, and 35.70 +/- 12.12% for T0, T1, and T2, respectively. The proportion of non-capacitated sperm also differed significantly among treatments (p = 0.0179), with values of 25.46 +/- 13.92%, 20.28 +/- 12.03%, and 23.13 +/- 11.54%, respectively. In contrast, the proportions of capacitated and acrosome-reacted sperm did not differ significantly among treatments (p = 0.642 and p = 0.236, respectively). These findings indicate that sexing using the egg-white sedimentation method affected post-thaw sperm motility and the proportion of non-capacitated sperm, while the proportions of capacitated and acrosome-reacted sperm remained comparable among treatments.
Keywords: Holstein-Friesian- Frozen semen- Sexed semen- Motility- Capacitation status- Sperm
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