Adaptation Pattern of Trembesi (Samanea saman Jacq. Merr.) seedling to lead expose at nutrient culture based on the characteristics of organic acids Luluk Setyaningsih 1)*, Mohammad Alif Syahrizki2), Mamay Maslahat 2)
1)Faculty of Forestry, Nusa Bangsa University. Jl. KH Sholeh Iskandar km 4 Cimanggu Tanah Sereal, Bogor. Indonesia. 16166
2)Faculty of Math and Natural Science, Nusa Bangsa University. Jl. KH Sholeh Iskandar km 4 Cimanggu Tanah Sereal, Bogor. Indonesia. 16166
Abstract
The aim of this study was to determine the adaptation pattern of trembesi (Samanea saman Jacq. Merr) seedlings to lead (Pb) exposure based on the characteristics of organic acids content. Trembesi seedling was grown on nutrient culture with Pb treatment of 0.5, 1.5 and 2.5 mM for 7 days. Percentage of seedling life was counted and the organic acids content was observed using High Performance Liquid Chromatography (HPLC). The results showed that the life percentage of trembesi seedling exposed to lead up to 1.5mM reached 100%, but it decreased to 60% when it was exposed to 2.5 mM of lead. Trembesi seedlings produced all three organic acids investigated as oxalic, malic and citric acid. The increase of lead exposure from 0.5 to 1.5 and 2.5 mM, caused significantly increase of oxalic acid exudation from 0.346 to 0.545 and 0.668 μg / mL. At the same time it also induced accumulation of citric acid from 46.4481 to 63,668 and 83,516 μg /g. This shows that trembesi is adapted to lead by combination of external and internal mechanisms involving organic acids.
