Prevalence, virulence and antibiotic resistance of Listeria monocytogenes isolated from retail sushi in Indonesia Yusma Yennie (a*), Gunawan (a), Fairdiana Andayani (a), Arifah Kusmarwati (a)
a) Research Center for Marine and Fisheries Product and Biotechnology, Ministry of Marine Affairs and Fisheries
*yenni.yusma[at]gmail.com
Abstract
Listeria monocytogenes is a foodborne pathogen which cause listeriosis and associated with raw ready to eat food such as sushi. The objective of this research was to determine the prevalence of Listeria monocytogenes, their virulence and antibiotic resistance. A total 120 sushi samples were collected from 111 retail outlets (restaurant and supermarket) have been confirmed Listeria monocytogenes by PCR method using virulence genes (hlyA, actA, and iap). The antibiotic resistance of Listeria monocytogenes isolates was evaluated with disc diffusion method. The prevalence of Listeria monocytogenes isolated from sushi was 14.2% (17/120 samples) and the hlyA, actA, and iap virulence genes were detected in 17, 13, 5 Listeria monocytogenes isolates , respectively. The highest resistance Listeria monocytogenes isolates was observed against erythromycin (37.5%), vancomycin and streptomycin (31.3%), tetracyline and ampicillin (18.8%) , and chloramphenicol (6.3%). Six out of 16 Listeria monocytogenes isolates were resistant againts 2 or more antibiotic (multidrug resistance).
