Rapid Detection of Porcine Protein Content in School Kids Snacks using Immunochromatographic Strip Rosy Hutami (a*), Rico Fernando Theo (a), Endrianur Rahman Zain (b), Noli Novidahlia (a)
a) Study Program of Food Technology, Faculty of Halal Food Science, Djuanda University, Kabupaten Bogor 16720, West Java, Indonesia
*rosy.hutami[at]unida.ac.id
b)Study Program of Management, Muhammadiyyah Asahan Islamic Economics College, North Sumatera, Indonesia
Abstract
Recently, halal food authentication becomes one of the food security requirements. Papers concerning immunochromatography in halal topics were only including identification of pork protein in raw and cooked pork meat, pork gelatin, fresh beef, raw chicken meat, and meatballs. The limited research on the halalness of school kids snacks both inside and outside the country and the critical potential use of processed meat in the ingredients of the school kids snacks make this topic important to investigate. This research aim was to identify the porcine protein contents in the school kids snacks in Bogor City using a porcine detection kit. This kit based on protein testing with the principle of conjugating gold colloids as markers with pig IgG polyclonal antibodies. This conjugate placed on the conjugate pad in the immunochromatographic "test strip" system. It can detect pig protein content in about 10 minutes, with 0.5% of the detection limit. The sampling method was purposive sampling based on the location of school snack sales closest to the pork sales source in Bogor. Samples analyzed were 24 samples consist of meatballs, sausages, and minced meat inside fried starch from six districts in Bogor. As a positive control, pork corned was used. The results obtained were the test lines were not shown for all samples, but it has appeared in the positive control. It concluded that all of the School Kids Snacks samples were negative from pig protein contents, and the kit was accurate to detect the protein content in the samples.
